UniProtKB - Q9R1L5 (MAST1_MOUSE)
Protein
Microtubule-associated serine/threonine-protein kinase 1
Gene
Mast1
Organism
Mus musculus (Mouse)
Status
Functioni
Microtubule-associated protein essential for correct brain development (PubMed:30449657). Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins.1 Publication2 Publications
Catalytic activityi
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 404 | ATPPROSITE-ProRule annotationBy similarity | 1 | |
Active sitei | 498 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 381 – 389 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- magnesium ion binding Source: UniProtKB
- microtubule binding Source: UniProtKB
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- brain development Source: UniProtKB
- cytoskeleton organization Source: UniProtKB
- intracellular signal transduction Source: UniProtKB
- peptidyl-serine phosphorylation Source: GO_Central
- protein phosphorylation Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 3474 |
Names & Taxonomyi
Protein namesi | Recommended name: Microtubule-associated serine/threonine-protein kinase 1 (EC:2.7.11.1)Alternative name(s): Syntrophin-associated serine/threonine-protein kinase |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1861901, Mast1 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Plasma membrane
- Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Other locations
Note: Also localized in the soma of neurons (PubMed:30449657). Observed as punctate clusters in the processes of interneurons and along the cell body periphery. Colocalizes with syntrophins at the cell membrane.1 Publication
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- axon Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB-KW
- dendrite Source: UniProtKB-SubCell
- membrane Source: UniProtKB
- neuron projection Source: UniProtKB
- neuronal cell body Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Disruption phenotypei
Knockout mice do not show morphological defects.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 279 | Missing : Mutant heterozygous mice have a thicker corpus callosum, hypoplastic cortex and cerebellum, increased neuronal apoptosis, and decreased protein levels. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086310 | 1 – 1570 | Microtubule-associated serine/threonine-protein kinase 1Add BLAST | 1570 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 90 | PhosphoserineCombined sources | 1 | |
Modified residuei | 139 | PhosphoserineCombined sources | 1 | |
Modified residuei | 167 | PhosphoserineCombined sources | 1 | |
Modified residuei | 346 | PhosphoserineBy similarity | 1 | |
Modified residuei | 351 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 689 | PhosphoserineBy similarity | 1 | |
Modified residuei | 895 | PhosphoserineCombined sources | 1 | |
Modified residuei | 954 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1414 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | Q9R1L5 |
PaxDbi | Q9R1L5 |
PeptideAtlasi | Q9R1L5 |
PRIDEi | Q9R1L5 |
ProteomicsDBi | 292091 |
PTM databases
iPTMneti | Q9R1L5 |
PhosphoSitePlusi | Q9R1L5 |
SwissPalmi | Q9R1L5 |
Expressioni
Tissue specificityi
Expressed in the brain (PubMed:30449657). Expressed in the developing cortical plate, the intermediate zone and corpus callosal fibers that cross the midline (PubMed:30449657). Detected at low levels in the testis, liver and spleen (PubMed:30449657). Expressed in proximity to neuronal nuclei throughout the cortex and cerebellum, and in the vascular endothelium. Also detected in ependymal cells, the choroid plexus, and in developing spermatid acrosomes.2 Publications
Developmental stagei
Expression in the brain begins at 12.5 dpc, peaks at 16.5 dpc, and decreases postnatally.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000053693, Expressed in cerebellar cortex and 225 other tissues |
ExpressionAtlasi | Q9R1L5, baseline and differential |
Genevisiblei | Q9R1L5, MM |
Interactioni
Subunit structurei
Interacts with the microtubules (PubMed:30449657).
Part of a low affinity complex that associates with, but is distinct from, the postsynaptic density.
Interacts with SNTB2.
2 PublicationsBinary interactionsi
Q9R1L5
With | #Exp. | IntAct |
---|---|---|
PTEN [P60484] from Homo sapiens. | 3 | EBI-491771,EBI-696162 |
GO - Molecular functioni
- microtubule binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 208038, 6 interactors |
IntActi | Q9R1L5, 3 interactors |
MINTi | Q9R1L5 |
STRINGi | 10090.ENSMUSP00000105363 |
Miscellaneous databases
RNActi | Q9R1L5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 375 – 648 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 274 | |
Domaini | 650 – 721 | AGC-kinase C-terminalPROSITE-ProRule annotationAdd BLAST | 72 | |
Domaini | 968 – 1056 | PDZPROSITE-ProRule annotationAdd BLAST | 89 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0605, Eukaryota KOG0606, Eukaryota |
GeneTreei | ENSGT00940000157700 |
HOGENOMi | CLU_000288_9_0_1 |
InParanoidi | Q9R1L5 |
OMAi | HIIWHVE |
OrthoDBi | 323328at2759 |
PhylomeDBi | Q9R1L5 |
TreeFami | TF313149 |
Family and domain databases
CDDi | cd05609, STKc_MAST, 1 hit |
Gene3Di | 1.20.1480.20, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR015022, MA_Ser/Thr_Kinase_dom IPR037711, MAST IPR028777, MAST1 IPR023142, MAST_pre-PK_dom_sf IPR001478, PDZ IPR041489, PDZ_6 IPR036034, PDZ_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR24356:SF150, PTHR24356:SF150, 1 hit |
Pfami | View protein in Pfam PF08926, DUF1908, 1 hit PF17820, PDZ_6, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF140482, SSF140482, 1 hit SSF50156, SSF50156, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS50106, PDZ, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9R1L5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDSLWTALS NFSMPSFPGG SMFRRTKSCR TSNRKSLILT STSPTLPRPH
60 70 80 90 100
SPLPGHLGSS PLDSPRNFSP NTPAHFSFAS SRRADGRRWS LASLPSSGYG
110 120 130 140 150
TNTPSSTVSS SCSSQERLHQ LPYQPTVDEL HFLSKHFGST ESITDEDGGR
160 170 180 190 200
RSPAVRPRSR SLSPGRSPSS YDNEIVMMNH VYKERFPKAT AQMEEKLRDF
210 220 230 240 250
ARAYEPDSVL PLADGVLSFI HHQIIELARD CLTKSRDGLI TTVYFYELQE
260 270 280 290 300
NLEKLLQDAY ERSESLEVAF VTQLVKKLLI IISRPARLLE CLEFNPEEFY
310 320 330 340 350
HLLEAAEGHA KEGHLVKTDI PRYIIRQLGL TRDPFPDVVR LEEQDSGGSN
360 370 380 390 400
TPEQDDTSEG RSSTSKAKKP PGESDFDTIK LISNGAYGAV YLVRHRDTRQ
410 420 430 440 450
RFAMKKINKQ NLILRNQIQQ AFVERDILTF AENPFVVGMF CSFETRRHLC
460 470 480 490 500
MVMEYVEGGD CATLLKNIGA LPVEMARMYF AETVLALEYL HNYGIVHRDL
510 520 530 540 550
KPDNLLITSM GHIKLTDFGL SKMGLMSLTT NLYEGHIEKD AREFLDKQVC
560 570 580 590 600
GTPEYIAPEV ILRQGYGKPV DWWAMGIILY EFLVGCVPFF GDTPEELFGQ
610 620 630 640 650
VISDDILWPE GDEALPTDAQ LLISSLLQTN PLVRLGAGGA FEVKQHSFFR
660 670 680 690 700
DLDWTGLLRQ KAEFIPHLES EDDTSYFDTR SDRYHHVNSY DEDDTTEEEP
710 720 730 740 750
VEIRQFSSCS PRFSKVYSSM EQLSQHEPKT PVSASGASKR DPSAKGPEEK
760 770 780 790 800
VAGKREGLGG LTLREKTWRG GSPEIKRFSA SEASFLEGEA SPPLGARRRF
810 820 830 840 850
SALLEPSRFT APQEDEDEAR LRRPPRPSSD PPSSLDTRVP KEAVQGEGTS
860 870 880 890 900
TPGEPEATER SHPGDLGPPS KDGDPSGPRA TNDLVLRRAR HQQLSGDLAV
910 920 930 940 950
EKRPSRTGGK VIKSASATAL SVMIPAVDPH GGSPLASPMS PRSLSSNPSS
960 970 980 990 1000
RDSSPSRDYS PAVSGLRSPI TIQRSGKKYG FTLRAIRVYM GDSDVYSVHH
1010 1020 1030 1040 1050
IVWHVEEGGP AQEAGLCAGD LITHVNGEPV HGMVHPEVVE LILKSGNKVA
1060 1070 1080 1090 1100
VTTTPFENTS IRIGPARRSS YKAKMARRNK RPSAKDGQES KKRSSLFRKI
1110 1120 1130 1140 1150
TKQSNLLHTS RSLSSLNRSL SSSDSLPGSP THGLPARSPT HSYRSTPDSA
1160 1170 1180 1190 1200
YLGASSQSSS PASSTPNSPA SSASHHIRPS TLHGLSPKLH RQYRSARCKS
1210 1220 1230 1240 1250
AGNIPLSPLA HTPSPTQASP PPLPGHTVGS SHTTQSFPAK LHSSPPVVRP
1260 1270 1280 1290 1300
RPKSAEPPRS PLLKRVQSAE KLGASLGADK KGALRKHSLE VGHPDFRKDF
1310 1320 1330 1340 1350
HGELALHSLA ESDGETPPIE GPGATRQVAV RRLGRQESPL SLGADPLLPD
1360 1370 1380 1390 1400
GVQRPMASSK EDSAGGTEAC TPPRATTPGS RTLERDSGCT RHQSVQTEDG
1410 1420 1430 1440 1450
PGGVARALAK AALSPVQEHE TGRRSSSGEA GTPPVPIVVE PARPGVKTQA
1460 1470 1480 1490 1500
PQPLGTDSKG LKEPVAQMPL MPDAPRGRER WVLEEVEERT TLSGLRSKPA
1510 1520 1530 1540 1550
SPKLSPDPQT PTLVPTKNVP RSAAPSVPPA SLMVPGTKPE AGLNSRCPAE
1560 1570
AVTPAGLTKT GAPSPASLGP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9Q6Q5 | E9Q6Q5_MOUSE | Microtubule-associated serine/threo... | Mast1 | 658 | Annotation score: |
Sequence cautioni
The sequence AAD50548 differs from that shown. Reason: Frameshift.Curated
The sequence BAC65693 differs from that shown. The sequence differs from that shown because it seems to be derived from a pre-mRNA.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 158 | R → P in AAD50548 (PubMed:10404183).Curated | 1 | |
Sequence conflicti | 197 – 198 | LR → P in AAD50548 (PubMed:10404183).Curated | 2 | |
Sequence conflicti | 647 | S → T in AAD50548 (PubMed:10404183).Curated | 1 | |
Sequence conflicti | 672 | D → E in AAD50548 (PubMed:10404183).Curated | 1 | |
Sequence conflicti | 1190 | H → L in AAD50548 (PubMed:10404183).Curated | 1 | |
Sequence conflicti | 1219 – 1250 | SPPPL…PVVRP → ARRRCQATRWAAHIHAELPC QTTYIATCVRR in AAD50548 (PubMed:10404183).CuratedAdd BLAST | 32 | |
Sequence conflicti | 1454 | L → V in AAD50548 (PubMed:10404183).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF077818 mRNA Translation: AAD50548.1 Frameshift. BC054524 mRNA Translation: AAH54524.1 AK122411 Transcribed RNA Translation: BAC65693.3 Sequence problems. |
CCDSi | CCDS40415.1 |
RefSeqi | NP_064329.2, NM_019945.2 |
Genome annotation databases
Ensembli | ENSMUST00000109741; ENSMUSP00000105363; ENSMUSG00000053693 |
GeneIDi | 56527 |
KEGGi | mmu:56527 |
UCSCi | uc009mof.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF077818 mRNA Translation: AAD50548.1 Frameshift. BC054524 mRNA Translation: AAH54524.1 AK122411 Transcribed RNA Translation: BAC65693.3 Sequence problems. |
CCDSi | CCDS40415.1 |
RefSeqi | NP_064329.2, NM_019945.2 |
3D structure databases
SMRi | Q9R1L5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208038, 6 interactors |
IntActi | Q9R1L5, 3 interactors |
MINTi | Q9R1L5 |
STRINGi | 10090.ENSMUSP00000105363 |
PTM databases
iPTMneti | Q9R1L5 |
PhosphoSitePlusi | Q9R1L5 |
SwissPalmi | Q9R1L5 |
Proteomic databases
MaxQBi | Q9R1L5 |
PaxDbi | Q9R1L5 |
PeptideAtlasi | Q9R1L5 |
PRIDEi | Q9R1L5 |
ProteomicsDBi | 292091 |
Protocols and materials databases
Antibodypediai | 26218, 93 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000109741; ENSMUSP00000105363; ENSMUSG00000053693 |
GeneIDi | 56527 |
KEGGi | mmu:56527 |
UCSCi | uc009mof.1, mouse |
Organism-specific databases
CTDi | 22983 |
MGIi | MGI:1861901, Mast1 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0605, Eukaryota KOG0606, Eukaryota |
GeneTreei | ENSGT00940000157700 |
HOGENOMi | CLU_000288_9_0_1 |
InParanoidi | Q9R1L5 |
OMAi | HIIWHVE |
OrthoDBi | 323328at2759 |
PhylomeDBi | Q9R1L5 |
TreeFami | TF313149 |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 56527, 4 hits in 53 CRISPR screens |
ChiTaRSi | Mast1, mouse |
PROi | PR:Q9R1L5 |
RNActi | Q9R1L5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000053693, Expressed in cerebellar cortex and 225 other tissues |
ExpressionAtlasi | Q9R1L5, baseline and differential |
Genevisiblei | Q9R1L5, MM |
Family and domain databases
CDDi | cd05609, STKc_MAST, 1 hit |
Gene3Di | 1.20.1480.20, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR000961, AGC-kinase_C IPR011009, Kinase-like_dom_sf IPR015022, MA_Ser/Thr_Kinase_dom IPR037711, MAST IPR028777, MAST1 IPR023142, MAST_pre-PK_dom_sf IPR001478, PDZ IPR041489, PDZ_6 IPR036034, PDZ_sf IPR000719, Prot_kinase_dom IPR008271, Ser/Thr_kinase_AS |
PANTHERi | PTHR24356:SF150, PTHR24356:SF150, 1 hit |
Pfami | View protein in Pfam PF08926, DUF1908, 1 hit PF17820, PDZ_6, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit SM00220, S_TKc, 1 hit |
SUPFAMi | SSF140482, SSF140482, 1 hit SSF50156, SSF50156, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51285, AGC_KINASE_CTER, 1 hit PS50106, PDZ, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAST1_MOUSE | |
Accessioni | Q9R1L5Primary (citable) accession number: Q9R1L5 Secondary accession number(s): Q7TQG9, Q80TN0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | September 11, 2007 | |
Last modified: | April 7, 2021 | |
This is version 170 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families