UniProtKB - Q9R1C7 (PR40A_MOUSE)
Pre-mRNA-processing factor 40 homolog A
Prpf40a
Functioni
Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing.
1 PublicationGO - Molecular functioni
- proline-rich region binding Source: MGI
- RNA binding Source: GO_Central
GO - Biological processi
- cell migration Source: UniProtKB
- cytoskeleton organization Source: UniProtKB
- mRNA cis splicing, via spliceosome Source: InterPro
- mRNA splicing, via spliceosome Source: GO_Central
- regulation of cell shape Source: UniProtKB
- regulation of cytokinesis Source: UniProtKB
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-processing factor 40 homolog AAlternative name(s): Formin-binding protein 11 Short name: FBP-11 Formin-binding protein 3 |
Gene namesi | Name:Prpf40a Synonyms:Fbp11, Fnbp3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1860512, Prpf40a |
VEuPathDBi | HostDB:ENSMUSG00000061136 |
Subcellular locationi
Nucleus
Note: Colocalizes with AKAP8L in the nuclear matrix.
Nucleus
- nuclear matrix Source: UniProtKB
- nuclear speck Source: MGI
- nucleus Source: MGI
- U1 snRNP Source: GO_Central
- U2-type prespliceosome Source: GO_Central
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076086 | 1 – 953 | Pre-mRNA-processing factor 40 homolog AAdd BLAST | 953 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 34 | PhosphoserineCombined sources | 1 | |
Modified residuei | 151 | PhosphoserineBy similarity | 1 | |
Cross-linki | 191 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 196 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 241 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 341 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 369 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 371 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 372 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 719 | PhosphoserineBy similarity | 1 | |
Modified residuei | 783 | PhosphoserineBy similarity | 1 | |
Modified residuei | 879 | PhosphoserineBy similarity | 1 | |
Modified residuei | 881 | PhosphoserineBy similarity | 1 | |
Modified residuei | 884 | PhosphoserineBy similarity | 1 | |
Modified residuei | 928 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 929 | PhosphoserineCombined sources | 1 | |
Modified residuei | 931 | PhosphoserineCombined sources | 1 | |
Modified residuei | 934 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9R1C7 |
jPOSTi | Q9R1C7 |
MaxQBi | Q9R1C7 |
PaxDbi | Q9R1C7 |
PeptideAtlasi | Q9R1C7 |
PRIDEi | Q9R1C7 |
ProteomicsDBi | 289391 [Q9R1C7-1] 289392 [Q9R1C7-2] |
PTM databases
iPTMneti | Q9R1C7 |
PhosphoSitePlusi | Q9R1C7 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000061136, Expressed in forelimb bud and 318 other tissues |
ExpressionAtlasi | Q9R1C7, baseline and differential |
Genevisiblei | Q9R1C7, MM |
Interactioni
Subunit structurei
Interacts with the N-terminus of HTT and with the phosphorylated C-terminal domain of POLR2A (By similarity).
Interacts with AKAP8L, SF1, SRPK1, ENAH, ATBF1 and MECP2.
Interacts through the WW domains with formin proline-rich regions and with WASL/N-WASP.
By similarity4 PublicationsBinary interactionsi
Q9R1C7
With | #Exp. | IntAct |
---|---|---|
AKAP8L [Q9ULX6] from Homo sapiens. | 3 | EBI-645554,EBI-357530 |
GO - Molecular functioni
- proline-rich region binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207833, 28 interactors |
ELMi | Q9R1C7 |
IntActi | Q9R1C7, 5 interactors |
MINTi | Q9R1C7 |
STRINGi | 10090.ENSMUSP00000075655 |
Miscellaneous databases
RNActi | Q9R1C7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 140 – 173 | WW 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 181 – 214 | WW 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 389 – 443 | FF 1Add BLAST | 55 | |
Domaini | 456 – 510 | FF 2Add BLAST | 55 | |
Domaini | 523 – 583 | FF 3Add BLAST | 61 | |
Domaini | 603 – 663 | FF 4Add BLAST | 61 | |
Domaini | 668 – 723 | FF 5Add BLAST | 56 | |
Domaini | 738 – 795 | FF 6Add BLAST | 58 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 40 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 348 – 382 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 799 – 953 | DisorderedSequence analysisAdd BLAST | 155 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 348 – 362 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 364 – 382 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 803 – 823 | Basic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 824 – 862 | Basic and acidic residuesSequence analysisAdd BLAST | 39 | |
Compositional biasi | 863 – 877 | Basic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 878 – 923 | Basic and acidic residuesSequence analysisAdd BLAST | 46 | |
Compositional biasi | 933 – 953 | Basic and acidic residuesSequence analysisAdd BLAST | 21 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0152, Eukaryota |
GeneTreei | ENSGT00930000150980 |
HOGENOMi | CLU_005825_0_0_1 |
InParanoidi | Q9R1C7 |
OMAi | HNVTTKE |
OrthoDBi | 1112854at2759 |
PhylomeDBi | Q9R1C7 |
TreeFami | TF318732 |
Family and domain databases
CDDi | cd00201, WW, 2 hits |
Gene3Di | 1.10.10.440, 5 hits |
InterProi | View protein in InterPro IPR002713, FF_domain IPR036517, FF_domain_sf IPR039726, Prp40-like IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR11864, PTHR11864, 1 hit |
Pfami | View protein in Pfam PF01846, FF, 5 hits PF00397, WW, 2 hits |
SMARTi | View protein in SMART SM00441, FF, 5 hits SM00456, WW, 2 hits |
SUPFAMi | SSF51045, SSF51045, 2 hits SSF81698, SSF81698, 5 hits |
PROSITEi | View protein in PROSITE PS51676, FF, 6 hits PS01159, WW_DOMAIN_1, 2 hits PS50020, WW_DOMAIN_2, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRPGTGAERG GLMVSEMESQ PPSRGPGDGE RRLSGSNLCS SSWVSADGFL
60 70 80 90 100
RRRPSMGHPG MHYAPMGMHP MGQRANMPPV PHGMMPQMMP PMGGPPMGQM
110 120 130 140 150
PGMMSSVMSG MMMSHMSQAS MQPALPPGVN SMDVAAGAAS GAKSMWTEHK
160 170 180 190 200
SPDGRTYYYN TETKQSTWEK PDDLKTPAEQ LLSKCPWKEY KSDSGKPYYY
210 220 230 240 250
NSQTKESRWA KPKELEDLEG YQNTIVAGGL ITKSNLHAMI KAEESSKQEE
260 270 280 290 300
CTTASTAPVP TTEIPTTMST MAAAEAAAAV VAAAAAAAAA ANANTSTTPT
310 320 330 340 350
NTVGSVPVAP EPEVTSIVAT AVDNENTVTV STEEQAQLAN TTAIQDLSGD
360 370 380 390 400
ISSNTGEEPA KQETVSDFTP KKEEEESQPA KKTYTWNTKE EAKQAFKELL
410 420 430 440 450
KEKRVPSNAS WEQAMKMIIN DPRYSALAKL SEKKQAFNAY KVQTEKEEKE
460 470 480 490 500
EARSKYKEAK ESFQRFLENH EKMTSTTRYK KAEQMFGEME VWNAISERDR
510 520 530 540 550
LEIYEDVLFF LSKKEKEQAK QLRKRNWEAL KNILDNMANV TYSTTWSEAQ
560 570 580 590 600
QYLMDNPTFA EDEELQNMDK EDALICFEEH IRALEKEEEE EKQKTLLRER
610 620 630 640 650
RRQRKNRESF QIFLDELHEH GQLHSMSSWM ELYPTISSDI RFTNMLGQPG
660 670 680 690 700
STALDLFKFY VEDLKARYHD EKKIIKDILK DKGFVVEVNT TFEDFVAIIS
710 720 730 740 750
STKRSTTLDA GNIKLAFNSL LEKAEARERE REKEEARKMK RKESAFKSML
760 770 780 790 800
KQATPPIELD AVWEDIRERF VKEPAFEDIT LESERKRIFK DFMHVLEHEC
810 820 830 840 850
QHHHSKNKKH SKKSKKHHRK RSRSRSGSES DDDDSHSKKK RQRSESHSAS
860 870 880 890 900
ERSSSAESER SYKKSKKHKK KSKKRRHKSD SPESDTEREK DKKEKDRDSE
910 920 930 940 950
KDRSRQRSES KHKSPKKKTG KDSGNWDTSG SELSEGELEK RRRTLLEQLD
DDQ
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GRU8 | A0A1B0GRU8_MOUSE | Pre-mRNA-processing factor 40 homol... | Prpf40a | 926 | Annotation score: | ||
A0A6Q6PUT5 | A0A6Q6PUT5_MOUSE | Pre-mRNA-processing factor 40 homol... | Prpf40a | 966 | Annotation score: | ||
A0A1B0GSF1 | A0A1B0GSF1_MOUSE | Pre-mRNA-processing factor 40 homol... | Prpf40a | 144 | Annotation score: | ||
A0A1B0GSH6 | A0A1B0GSH6_MOUSE | Pre-mRNA-processing factor 40 homol... | Prpf40a | 102 | Annotation score: | ||
A0A1B0GT17 | A0A1B0GT17_MOUSE | Pre-mRNA-processing factor 40 homol... | Prpf40a | 84 | Annotation score: | ||
A2AQW6 | A2AQW6_MOUSE | Pre-mRNA-processing factor 40 homol... | Prpf40a | 100 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 334 | E → A in BAC40061 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 355 | T → I in BAC40061 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008049 | 14 – 55 | Missing in isoform 2. 1 PublicationAdd BLAST | 42 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF135439 mRNA Translation: AAD39463.1 AK041205 mRNA Translation: BAC30863.1 AK051375 mRNA Translation: BAC34617.1 AK087963 mRNA Translation: BAC40061.2 U40747 mRNA Translation: AAC52475.1 |
PIRi | S64713 |
RefSeqi | NP_061255.1, NM_018785.2 |
Genome annotation databases
Ensembli | ENSMUST00000239398; ENSMUSP00000159339; ENSMUSG00000061136 [Q9R1C7-1] |
GeneIDi | 56194 |
KEGGi | mmu:56194 |
UCSCi | uc008jrk.2, mouse [Q9R1C7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF135439 mRNA Translation: AAD39463.1 AK041205 mRNA Translation: BAC30863.1 AK051375 mRNA Translation: BAC34617.1 AK087963 mRNA Translation: BAC40061.2 U40747 mRNA Translation: AAC52475.1 |
PIRi | S64713 |
RefSeqi | NP_061255.1, NM_018785.2 |
3D structure databases
BMRBi | Q9R1C7 |
SMRi | Q9R1C7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207833, 28 interactors |
ELMi | Q9R1C7 |
IntActi | Q9R1C7, 5 interactors |
MINTi | Q9R1C7 |
STRINGi | 10090.ENSMUSP00000075655 |
PTM databases
iPTMneti | Q9R1C7 |
PhosphoSitePlusi | Q9R1C7 |
Proteomic databases
EPDi | Q9R1C7 |
jPOSTi | Q9R1C7 |
MaxQBi | Q9R1C7 |
PaxDbi | Q9R1C7 |
PeptideAtlasi | Q9R1C7 |
PRIDEi | Q9R1C7 |
ProteomicsDBi | 289391 [Q9R1C7-1] 289392 [Q9R1C7-2] |
Protocols and materials databases
Antibodypediai | 33687, 91 antibodies from 18 providers |
DNASUi | 56194 |
Genome annotation databases
Ensembli | ENSMUST00000239398; ENSMUSP00000159339; ENSMUSG00000061136 [Q9R1C7-1] |
GeneIDi | 56194 |
KEGGi | mmu:56194 |
UCSCi | uc008jrk.2, mouse [Q9R1C7-1] |
Organism-specific databases
CTDi | 55660 |
MGIi | MGI:1860512, Prpf40a |
VEuPathDBi | HostDB:ENSMUSG00000061136 |
Phylogenomic databases
eggNOGi | KOG0152, Eukaryota |
GeneTreei | ENSGT00930000150980 |
HOGENOMi | CLU_005825_0_0_1 |
InParanoidi | Q9R1C7 |
OMAi | HNVTTKE |
OrthoDBi | 1112854at2759 |
PhylomeDBi | Q9R1C7 |
TreeFami | TF318732 |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 56194, 20 hits in 65 CRISPR screens |
ChiTaRSi | Prpf40a, mouse |
PROi | PR:Q9R1C7 |
RNActi | Q9R1C7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000061136, Expressed in forelimb bud and 318 other tissues |
ExpressionAtlasi | Q9R1C7, baseline and differential |
Genevisiblei | Q9R1C7, MM |
Family and domain databases
CDDi | cd00201, WW, 2 hits |
Gene3Di | 1.10.10.440, 5 hits |
InterProi | View protein in InterPro IPR002713, FF_domain IPR036517, FF_domain_sf IPR039726, Prp40-like IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR11864, PTHR11864, 1 hit |
Pfami | View protein in Pfam PF01846, FF, 5 hits PF00397, WW, 2 hits |
SMARTi | View protein in SMART SM00441, FF, 5 hits SM00456, WW, 2 hits |
SUPFAMi | SSF51045, SSF51045, 2 hits SSF81698, SSF81698, 5 hits |
PROSITEi | View protein in PROSITE PS51676, FF, 6 hits PS01159, WW_DOMAIN_1, 2 hits PS50020, WW_DOMAIN_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PR40A_MOUSE | |
Accessioni | Q9R1C7Primary (citable) accession number: Q9R1C7 Secondary accession number(s): Q61049 Q8C2U1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 22, 2003 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 172 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families