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Protein

Pre-mRNA-processing factor 40 homolog A

Gene

Prpf40a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to WASL/N-WASP and suppresses its translocation from the nucleus to the cytoplasm, thereby inhibiting its cytoplasmic function. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape and migration. May play a role in cytokinesis. May be involved in pre-mRNA splicing.1 Publication

GO - Molecular functioni

  • proline-rich region binding Source: MGI
  • RNA binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 40 homolog A
Alternative name(s):
Formin-binding protein 11
Short name:
FBP-11
Formin-binding protein 3
Gene namesi
Name:Prpf40a
Synonyms:Fbp11, Fnbp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1860512 Prpf40a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760861 – 953Pre-mRNA-processing factor 40 homolog AAdd BLAST953

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9Omega-N-methylarginineCombined sources1
Modified residuei34PhosphoserineCombined sources1
Modified residuei151PhosphoserineBy similarity1
Cross-linki191Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei196N6-acetyllysineCombined sources1
Cross-linki241Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei341PhosphothreonineBy similarity1
Modified residuei369PhosphothreonineBy similarity1
Cross-linki371Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki372Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei719PhosphoserineBy similarity1
Modified residuei783PhosphoserineBy similarity1
Modified residuei879PhosphoserineBy similarity1
Modified residuei881PhosphoserineBy similarity1
Modified residuei884PhosphoserineBy similarity1
Modified residuei928PhosphothreonineCombined sources1
Modified residuei929PhosphoserineCombined sources1
Modified residuei931PhosphoserineCombined sources1
Modified residuei934PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9R1C7
PaxDbiQ9R1C7
PeptideAtlasiQ9R1C7
PRIDEiQ9R1C7

PTM databases

iPTMnetiQ9R1C7
PhosphoSitePlusiQ9R1C7

Expressioni

Gene expression databases

BgeeiENSMUSG00000061136 Expressed in 298 organ(s), highest expression level in testis
ExpressionAtlasiQ9R1C7 baseline and differential
GenevisibleiQ9R1C7 MM

Interactioni

Subunit structurei

Interacts with the N-terminus of HTT and with the phosphorylated C-terminal domain of POLR2A (By similarity). Interacts with AKAP8L, SF1, SRPK1, CARD8, ATBF1 and MECP2. Interacts through the WW domains with formin proline-rich regions and with WASL/N-WASP.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AKAP8LQ9ULX63EBI-645554,EBI-357530From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207833, 7 interactors
ELMiQ9R1C7
IntActiQ9R1C7, 6 interactors
MINTiQ9R1C7
STRINGi10090.ENSMUSP00000075655

Structurei

3D structure databases

ProteinModelPortaliQ9R1C7
SMRiQ9R1C7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 173WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini181 – 214WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini389 – 443FF 1Add BLAST55
Domaini456 – 510FF 2Add BLAST55
Domaini523 – 583FF 3Add BLAST61
Domaini603 – 663FF 4Add BLAST61
Domaini668 – 723FF 5Add BLAST56
Domaini738 – 795FF 6Add BLAST58

Domaini

The WW domains are essential for localization to nuclear speckles.

Sequence similaritiesi

Belongs to the PRPF40 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0152 Eukaryota
COG5104 LUCA
GeneTreeiENSGT00930000150980
HOGENOMiHOG000231802
HOVERGENiHBG059634
InParanoidiQ9R1C7
KOiK12821
OMAiIWEDFIK
OrthoDBiEOG091G06F8
PhylomeDBiQ9R1C7
TreeFamiTF318732

Family and domain databases

CDDicd00201 WW, 2 hits
Gene3Di1.10.10.440, 5 hits
InterProiView protein in InterPro
IPR002713 FF_domain
IPR036517 FF_domain_sf
IPR039726 Prp40/Urn1
IPR001202 WW_dom
IPR036020 WW_dom_sf
PANTHERiPTHR11864 PTHR11864, 1 hit
PfamiView protein in Pfam
PF01846 FF, 5 hits
PF00397 WW, 2 hits
SMARTiView protein in SMART
SM00441 FF, 5 hits
SM00456 WW, 2 hits
SUPFAMiSSF51045 SSF51045, 2 hits
SSF81698 SSF81698, 5 hits
PROSITEiView protein in PROSITE
PS51676 FF, 6 hits
PS01159 WW_DOMAIN_1, 2 hits
PS50020 WW_DOMAIN_2, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9R1C7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRPGTGAERG GLMVSEMESQ PPSRGPGDGE RRLSGSNLCS SSWVSADGFL
60 70 80 90 100
RRRPSMGHPG MHYAPMGMHP MGQRANMPPV PHGMMPQMMP PMGGPPMGQM
110 120 130 140 150
PGMMSSVMSG MMMSHMSQAS MQPALPPGVN SMDVAAGAAS GAKSMWTEHK
160 170 180 190 200
SPDGRTYYYN TETKQSTWEK PDDLKTPAEQ LLSKCPWKEY KSDSGKPYYY
210 220 230 240 250
NSQTKESRWA KPKELEDLEG YQNTIVAGGL ITKSNLHAMI KAEESSKQEE
260 270 280 290 300
CTTASTAPVP TTEIPTTMST MAAAEAAAAV VAAAAAAAAA ANANTSTTPT
310 320 330 340 350
NTVGSVPVAP EPEVTSIVAT AVDNENTVTV STEEQAQLAN TTAIQDLSGD
360 370 380 390 400
ISSNTGEEPA KQETVSDFTP KKEEEESQPA KKTYTWNTKE EAKQAFKELL
410 420 430 440 450
KEKRVPSNAS WEQAMKMIIN DPRYSALAKL SEKKQAFNAY KVQTEKEEKE
460 470 480 490 500
EARSKYKEAK ESFQRFLENH EKMTSTTRYK KAEQMFGEME VWNAISERDR
510 520 530 540 550
LEIYEDVLFF LSKKEKEQAK QLRKRNWEAL KNILDNMANV TYSTTWSEAQ
560 570 580 590 600
QYLMDNPTFA EDEELQNMDK EDALICFEEH IRALEKEEEE EKQKTLLRER
610 620 630 640 650
RRQRKNRESF QIFLDELHEH GQLHSMSSWM ELYPTISSDI RFTNMLGQPG
660 670 680 690 700
STALDLFKFY VEDLKARYHD EKKIIKDILK DKGFVVEVNT TFEDFVAIIS
710 720 730 740 750
STKRSTTLDA GNIKLAFNSL LEKAEARERE REKEEARKMK RKESAFKSML
760 770 780 790 800
KQATPPIELD AVWEDIRERF VKEPAFEDIT LESERKRIFK DFMHVLEHEC
810 820 830 840 850
QHHHSKNKKH SKKSKKHHRK RSRSRSGSES DDDDSHSKKK RQRSESHSAS
860 870 880 890 900
ERSSSAESER SYKKSKKHKK KSKKRRHKSD SPESDTEREK DKKEKDRDSE
910 920 930 940 950
KDRSRQRSES KHKSPKKKTG KDSGNWDTSG SELSEGELEK RRRTLLEQLD

DDQ
Length:953
Mass (Da):108,481
Last modified:May 1, 2000 - v1
Checksum:i3C627AB7404D2285
GO
Isoform 2 (identifier: Q9R1C7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     14-55: Missing.

Note: No experimental confirmation available.
Show »
Length:911
Mass (Da):103,963
Checksum:i7BE2E454CC5365CA
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AQW6A2AQW6_MOUSE
Pre-mRNA-processing factor 40 homol...
Prpf40a
100Annotation score:
A0A1B0GRU8A0A1B0GRU8_MOUSE
Pre-mRNA-processing factor 40 homol...
Prpf40a
926Annotation score:
A0A1B0GSF1A0A1B0GSF1_MOUSE
Pre-mRNA-processing factor 40 homol...
Prpf40a
129Annotation score:
A0A1B0GT17A0A1B0GT17_MOUSE
Pre-mRNA-processing factor 40 homol...
Prpf40a
84Annotation score:
A0A1B0GSH6A0A1B0GSH6_MOUSE
Pre-mRNA-processing factor 40 homol...
Prpf40a
87Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti334E → A in BAC40061 (PubMed:16141072).Curated1
Sequence conflicti355T → I in BAC40061 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00804914 – 55Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135439 mRNA Translation: AAD39463.1
AK041205 mRNA Translation: BAC30863.1
AK051375 mRNA Translation: BAC34617.1
AK087963 mRNA Translation: BAC40061.2
U40747 mRNA Translation: AAC52475.1
CCDSiCCDS16038.1 [Q9R1C7-1]
PIRiS64713
RefSeqiNP_061255.1, NM_018785.2 [Q9R1C7-1]
UniGeneiMm.257474
Mm.392945
Mm.393219

Genome annotation databases

EnsembliENSMUST00000076313; ENSMUSP00000075655; ENSMUSG00000061136 [Q9R1C7-1]
ENSMUST00000210789; ENSMUSP00000147599; ENSMUSG00000061136 [Q9R1C7-2]
GeneIDi56194
KEGGimmu:56194
UCSCiuc008jrk.2 mouse [Q9R1C7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135439 mRNA Translation: AAD39463.1
AK041205 mRNA Translation: BAC30863.1
AK051375 mRNA Translation: BAC34617.1
AK087963 mRNA Translation: BAC40061.2
U40747 mRNA Translation: AAC52475.1
CCDSiCCDS16038.1 [Q9R1C7-1]
PIRiS64713
RefSeqiNP_061255.1, NM_018785.2 [Q9R1C7-1]
UniGeneiMm.257474
Mm.392945
Mm.393219

3D structure databases

ProteinModelPortaliQ9R1C7
SMRiQ9R1C7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207833, 7 interactors
ELMiQ9R1C7
IntActiQ9R1C7, 6 interactors
MINTiQ9R1C7
STRINGi10090.ENSMUSP00000075655

PTM databases

iPTMnetiQ9R1C7
PhosphoSitePlusiQ9R1C7

Proteomic databases

EPDiQ9R1C7
PaxDbiQ9R1C7
PeptideAtlasiQ9R1C7
PRIDEiQ9R1C7

Protocols and materials databases

DNASUi56194
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076313; ENSMUSP00000075655; ENSMUSG00000061136 [Q9R1C7-1]
ENSMUST00000210789; ENSMUSP00000147599; ENSMUSG00000061136 [Q9R1C7-2]
GeneIDi56194
KEGGimmu:56194
UCSCiuc008jrk.2 mouse [Q9R1C7-1]

Organism-specific databases

CTDi55660
MGIiMGI:1860512 Prpf40a

Phylogenomic databases

eggNOGiKOG0152 Eukaryota
COG5104 LUCA
GeneTreeiENSGT00930000150980
HOGENOMiHOG000231802
HOVERGENiHBG059634
InParanoidiQ9R1C7
KOiK12821
OMAiIWEDFIK
OrthoDBiEOG091G06F8
PhylomeDBiQ9R1C7
TreeFamiTF318732

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway

Miscellaneous databases

PROiPR:Q9R1C7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061136 Expressed in 298 organ(s), highest expression level in testis
ExpressionAtlasiQ9R1C7 baseline and differential
GenevisibleiQ9R1C7 MM

Family and domain databases

CDDicd00201 WW, 2 hits
Gene3Di1.10.10.440, 5 hits
InterProiView protein in InterPro
IPR002713 FF_domain
IPR036517 FF_domain_sf
IPR039726 Prp40/Urn1
IPR001202 WW_dom
IPR036020 WW_dom_sf
PANTHERiPTHR11864 PTHR11864, 1 hit
PfamiView protein in Pfam
PF01846 FF, 5 hits
PF00397 WW, 2 hits
SMARTiView protein in SMART
SM00441 FF, 5 hits
SM00456 WW, 2 hits
SUPFAMiSSF51045 SSF51045, 2 hits
SSF81698 SSF81698, 5 hits
PROSITEiView protein in PROSITE
PS51676 FF, 6 hits
PS01159 WW_DOMAIN_1, 2 hits
PS50020 WW_DOMAIN_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPR40A_MOUSE
AccessioniPrimary (citable) accession number: Q9R1C7
Secondary accession number(s): Q61049
, Q8BQ76, Q8BRW4, Q8C2U1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 22, 2003
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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