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Protein

Disintegrin and metalloproteinase domain-containing protein 18

Gene

Adam18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sperm surface membrane protein that may be involved in spermatogenesis and fertilization. This is a non catalytic metalloprotease-like protein.

GO - Molecular functioni

  • integrin binding Source: MGI
  • metalloendopeptidase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Spermatogenesis

Protein family/group databases

MEROPSiM12.958

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 18
Short name:
ADAM 18
Alternative name(s):
Disintegrin and metalloproteinase domain-containing protein 27
Short name:
ADAM 27
Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein III
Short name:
tMDC III
Gene namesi
Name:Adam18
Synonyms:Adam27, Dtgn3, Tmdc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:105986 Adam18

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini173 – 683ExtracellularSequence analysisAdd BLAST511
Transmembranei684 – 704HelicalSequence analysisAdd BLAST21
Topological domaini705 – 719CytoplasmicSequence analysisAdd BLAST15

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002910020 – 179Sequence analysisAdd BLAST160
ChainiPRO_0000029101180 – 719Disintegrin and metalloproteinase domain-containing protein 18Add BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi61N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi121N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi152N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi244N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi289 ↔ 373By similarity
Glycosylationi331N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi332 ↔ 357By similarity
Disulfide bondi334 ↔ 339By similarity
Glycosylationi356N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi405N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi447 ↔ 468By similarity
Glycosylationi607N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi614N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi620 ↔ 632By similarity
Glycosylationi621N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi626 ↔ 638By similarity
Disulfide bondi640 ↔ 649By similarity

Post-translational modificationi

The prodomain and the metalloprotease-like domain are cleaved during the epididymal maturation of the spermatozoa.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9R157
PRIDEiQ9R157

PTM databases

iPTMnetiQ9R157
PhosphoSitePlusiQ9R157

Expressioni

Tissue specificityi

Expressed specifically in testis.

Developmental stagei

Adult levels are reached by day 16 after birth.

Gene expression databases

BgeeiENSMUSG00000031552
CleanExiMM_ADAM18
ExpressionAtlasiQ9R157 baseline and differential
GenevisibleiQ9R157 MM

Interactioni

GO - Molecular functioni

  • integrin binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033957

Structurei

3D structure databases

ProteinModelPortaliQ9R157
SMRiQ9R157
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 378Peptidase M12BPROSITE-ProRule annotationAdd BLAST199
Domaini387 – 476DisintegrinPROSITE-ProRule annotationAdd BLAST90
Domaini616 – 650EGF-likePROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi477 – 615Cys-richAdd BLAST139

Domaini

A tripeptide motif (ECD) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3607 Eukaryota
ENOG410XX2M LUCA
GeneTreeiENSGT00910000144019
HOGENOMiHOG000230883
HOVERGENiHBG103628
InParanoidiQ9R157
KOiK16909
OMAiAGTPCRK
OrthoDBiEOG091G02F0
TreeFamiTF314733

Family and domain databases

CDDicd04269 ZnMc_adamalysin_II_like, 1 hit
Gene3Di3.40.390.10, 1 hit
4.10.70.10, 1 hit
InterProiView protein in InterPro
IPR006586 ADAM_Cys-rich
IPR001762 Disintegrin_dom
IPR036436 Disintegrin_dom_sf
IPR000742 EGF-like_dom
IPR024079 MetalloPept_cat_dom_sf
IPR001590 Peptidase_M12B
IPR002870 Peptidase_M12B_N
IPR034027 Reprolysin_adamalysin
PfamiView protein in Pfam
PF08516 ADAM_CR, 1 hit
PF00200 Disintegrin, 1 hit
PF01562 Pep_M12B_propep, 1 hit
PF01421 Reprolysin, 1 hit
SMARTiView protein in SMART
SM00608 ACR, 1 hit
SM00050 DISIN, 1 hit
SUPFAMiSSF57552 SSF57552, 1 hit
PROSITEiView protein in PROSITE
PS50215 ADAM_MEPRO, 1 hit
PS50214 DISINTEGRIN_2, 1 hit
PS50026 EGF_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLFILAEL AMLFARLDSE GICLHITVPQ KIEPRKGGDA EGKVTYVITI
60 70 80 90 100
DGKPYSLHLR NHSFLSQNFL VYTYNETGSL YSDSSHFLAH CHYRGYVDEV
110 120 130 140 150
PNSIVTLSIC SGLRGFLQLE NVSYGIEPLE SSARFEHIVY QVKSDSSMLA
160 170 180 190 200
GNDSHVWQID QLDKGHFNEQ DKNHSQLLPQ SLKLHIIVGK FLFDYMGSDI
210 220 230 240 250
MAITQKIFQI IGLVNAMLTQ LKLSVVLASL ELWSDKNHIS TDGNATDILQ
260 270 280 290 300
RLLDWKRDYL TLQSNEITHL LIYRRRPKYI GAASPGEICS KSYVAGVGMY
310 320 330 340 350
PEDIGLEGFS VVITQLIGLH IGLTYDDNIR NCSCPSAPCI MQQGALSSSG
360 370 380 390 400
KKTFSNCSLH DYMHYVSNFD TQCLGDLSNV HVLQPNQAVC GNGIMEAGEE
410 420 430 440 450
CDCGNETECQ FKECCDHETC RLKGSAQCGS GACCMPTCEL SASGTPCRKA
460 470 480 490 500
VDPECDFTEY CDGSSSHCVP DTFALNGHLC RLGSAYCYNG RCQALNDQCV
510 520 530 540 550
SLFGKGSQGA SYACFEKVNS PRENLANCDS KDSYSVPCGQ QDVLCGKLAC
560 570 580 590 600
FRPPKNYKSP SQSVVYSYVH DSVCLSILPG LSMRSDGRDS AYVADGTVCG
610 620 630 640 650
PQMYCINGTC KEVNFTGNDC NATKKCKGNG ICNNFGNCQC FPDYRPPDCN
660 670 680 690 700
LQIGSPGGSI DDGNTLRTES AFATKRLSKN EDSWVILGFF IFLPFIVTFL
710
VGIMKRNERK IVPQGEHKI
Length:719
Mass (Da):79,210
Last modified:July 27, 2011 - v2
Checksum:i9CD092E12625DA6D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145D → N in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti159I → T in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti169E → D in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti276R → H in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti386N → K in AAD48844 (PubMed:10395895).Curated1
Sequence conflicti577I → V in AAD48844 (PubMed:10395895).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF167405 mRNA Translation: AAD48844.1
BC051136 mRNA Translation: AAH51136.2
U06148 mRNA Translation: AAA18427.1
CCDSiCCDS22195.1
PIRiI48946
RefSeqiNP_034214.2, NM_010084.2
UniGeneiMm.89943

Genome annotation databases

EnsembliENSMUST00000033957; ENSMUSP00000033957; ENSMUSG00000031552
GeneIDi13524
KEGGimmu:13524
UCSCiuc009lfb.2 mouse

Similar proteinsi

Entry informationi

Entry nameiADA18_MOUSE
AccessioniPrimary (citable) accession number: Q9R157
Secondary accession number(s): Q60621, Q80Y08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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