Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transmembrane emp24 domain-containing protein 2

Gene

Tmed2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase-activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing (By similarity). Required for morphogenesis of embryo and placenta.By similarity1 Publication

Miscellaneous

Embryonic lethal for homozygotes with Glu-15 in the signal peptide. Animals do not show Tmed2 protein expression and also have reduced protein levels of Tmed7 and Tmed10. Prior to death at mid-gestation, embryos exhibit developmental delay, abnormal rostral-caudal elongation, randomized heart looping, and absence of the labyrinth layer of the placenta.

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1912420 Pre-NOTCH Processing in Golgi
R-MMU-204005 COPII-mediated vesicle transport
R-MMU-5694530 Cargo concentration in the ER
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 2
Alternative name(s):
COPI-coated vesicle membrane protein p24
Membrane protein p24A
Sid 394
p24 family protein beta-1
Short name:
p24beta1
Gene namesi
Name:Tmed2
Synonyms:Rnp24, Sid394
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1929269 Tmed2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 168LumenalSequence analysisAdd BLAST148
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 201CytoplasmicSequence analysisAdd BLAST12

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15L → E: Embryonic lethal. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001038221 – 201Transmembrane emp24 domain-containing protein 2Add BLAST181

Proteomic databases

EPDiQ9R0Q3
MaxQBiQ9R0Q3
PaxDbiQ9R0Q3
PeptideAtlasiQ9R0Q3
PRIDEiQ9R0Q3

PTM databases

iPTMnetiQ9R0Q3
PhosphoSitePlusiQ9R0Q3

Expressioni

Developmental stagei

Expressed during embryonal and placental development.1 Publication

Gene expression databases

BgeeiENSMUSG00000029390 Expressed in 300 organ(s), highest expression level in urothelium
CleanExiMM_TMED2
ExpressionAtlasiQ9R0Q3 baseline and differential
GenevisibleiQ9R0Q3 MM

Interactioni

Subunit structurei

Monomer and homodimer in the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment and Golgi. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED7, TMED9 and TMED10. Interacts (via GOLD domain) with TMED10 (via GOLD domain). Associates with the COPI vesicle coat (coatomer); TMED10:TMED2 heterotetramers are proposed to be involved in coatomer association. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED2. Interacts with SEC23A; indicative for an association of TMED2 with the COPII vesicle coat. Interacts with ARF1 and ARFGAP1. Interacts with CD59, SEC24A, SEC24B, SEC24C, SEC24D and ATLA1. Interacts with KDELR1; the interaction is decreased by KDEL ligand. Interacts with F2RL1; the interaction occurs at the Golgi apparatus. Interacts with CASR (immaturely glycosylated form); the interaction occurs in the endoplasmic reticulum-Golgi intermediate compartment or cis-Golgi. Interacts with F2RL1; the interaction occurs at the Golgi apparatus. Interacts with GORASP1 and GORASP2. Found in a complex composed at least of SURF4, TMED2 and TMED10 (By similarity).By similarity

Protein-protein interaction databases

BioGridi207909, 116 interactors
IntActiQ9R0Q3, 120 interactors
STRINGi10090.ENSMUSP00000054834

Structurei

3D structure databases

ProteinModelPortaliQ9R0Q3
SMRiQ9R0Q3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 112GOLDPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 181Interaction with F2RL1By similarityAdd BLAST181
Regioni118 – 157Required for TMED10 and TMED2 cis-Golgi network localizationBy similarityAdd BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili117 – 167Sequence analysisAdd BLAST51

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi194 – 201COPI vesicle coat-bindingSequence analysis8
Motifi194 – 195COPII vesicle coat-bindingSequence analysis2

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1692 Eukaryota
ENOG410XQCK LUCA
GeneTreeiENSGT00670000097656
HOGENOMiHOG000160229
HOVERGENiHBG105106
InParanoidiQ9R0Q3
KOiK20347
OMAiGRHEYCF
OrthoDBiEOG091G0L5D
PhylomeDBiQ9R0Q3
TreeFamiTF313000

Family and domain databases

InterProiView protein in InterPro
IPR009038 GOLD_dom
IPR036598 GOLD_dom_sf
IPR015720 TMP21-related
PANTHERiPTHR22811 PTHR22811, 1 hit
PfamiView protein in Pfam
PF01105 EMP24_GP25L, 1 hit
SMARTiView protein in SMART
SM01190 EMP24_GP25L, 1 hit
SUPFAMiSSF101576 SSF101576, 1 hit
PROSITEiView protein in PROSITE
PS50866 GOLD, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9R0Q3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVTLAELLAL LAALLATASG YFVSIDAHAE ECFFERVTSG TKMGLIFEVA
60 70 80 90 100
EGGFLDIDVE ITGPDNKGIY KGDRESSGKY TFAAHMDGTY KFCFSNRMST
110 120 130 140 150
MTPKIVMFTI DIGEAPKGQD METEAHQNKL EEMINELAVA MTAVKHEQEY
160 170 180 190 200
MEVRERIHRA INDNTNSRVV LWSFFEALVL VAMTLGQIYY LKRFFEVRRV

V
Length:201
Mass (Da):22,705
Last modified:May 1, 2000 - v1
Checksum:iF5DE259FC419CF85
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6V6T4F6V6T4_MOUSE
Transmembrane emp24 domain-containi...
Tmed2
176Annotation score:
Q8BPI2Q8BPI2_MOUSE
Transmembrane emp24 domain-containi...
Tmed2
112Annotation score:
A0A0G2JEB1A0A0G2JEB1_MOUSE
Transmembrane emp24 domain-containi...
Tmed2
55Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025218 mRNA Translation: BAA84689.1
BC083140 mRNA Translation: AAH83140.1
CCDSiCCDS19679.1
RefSeqiNP_062744.1, NM_019770.2
XP_003945495.1, XM_003945446.4
UniGeneiMm.325307
Mm.425279

Genome annotation databases

EnsembliENSMUST00000060226; ENSMUSP00000054834; ENSMUSG00000029390
GeneIDi100862175
56334
KEGGimmu:100862175
mmu:56334
UCSCiuc008zqa.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB025218 mRNA Translation: BAA84689.1
BC083140 mRNA Translation: AAH83140.1
CCDSiCCDS19679.1
RefSeqiNP_062744.1, NM_019770.2
XP_003945495.1, XM_003945446.4
UniGeneiMm.325307
Mm.425279

3D structure databases

ProteinModelPortaliQ9R0Q3
SMRiQ9R0Q3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207909, 116 interactors
IntActiQ9R0Q3, 120 interactors
STRINGi10090.ENSMUSP00000054834

PTM databases

iPTMnetiQ9R0Q3
PhosphoSitePlusiQ9R0Q3

Proteomic databases

EPDiQ9R0Q3
MaxQBiQ9R0Q3
PaxDbiQ9R0Q3
PeptideAtlasiQ9R0Q3
PRIDEiQ9R0Q3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060226; ENSMUSP00000054834; ENSMUSG00000029390
GeneIDi100862175
56334
KEGGimmu:100862175
mmu:56334
UCSCiuc008zqa.1 mouse

Organism-specific databases

CTDi10959
MGIiMGI:1929269 Tmed2

Phylogenomic databases

eggNOGiKOG1692 Eukaryota
ENOG410XQCK LUCA
GeneTreeiENSGT00670000097656
HOGENOMiHOG000160229
HOVERGENiHBG105106
InParanoidiQ9R0Q3
KOiK20347
OMAiGRHEYCF
OrthoDBiEOG091G0L5D
PhylomeDBiQ9R0Q3
TreeFamiTF313000

Enzyme and pathway databases

ReactomeiR-MMU-1912420 Pre-NOTCH Processing in Golgi
R-MMU-204005 COPII-mediated vesicle transport
R-MMU-5694530 Cargo concentration in the ER
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic

Miscellaneous databases

PROiPR:Q9R0Q3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029390 Expressed in 300 organ(s), highest expression level in urothelium
CleanExiMM_TMED2
ExpressionAtlasiQ9R0Q3 baseline and differential
GenevisibleiQ9R0Q3 MM

Family and domain databases

InterProiView protein in InterPro
IPR009038 GOLD_dom
IPR036598 GOLD_dom_sf
IPR015720 TMP21-related
PANTHERiPTHR22811 PTHR22811, 1 hit
PfamiView protein in Pfam
PF01105 EMP24_GP25L, 1 hit
SMARTiView protein in SMART
SM01190 EMP24_GP25L, 1 hit
SUPFAMiSSF101576 SSF101576, 1 hit
PROSITEiView protein in PROSITE
PS50866 GOLD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTMED2_MOUSE
AccessioniPrimary (citable) accession number: Q9R0Q3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again