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Protein

Synaptotagmin-9

Gene

Syt9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi251Calcium 1By similarity1
Metal bindingi251Calcium 2By similarity1
Metal bindingi257Calcium 1By similarity1
Metal bindingi309Calcium 1By similarity1
Metal bindingi309Calcium 2By similarity1
Metal bindingi310Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi311Calcium 1By similarity1
Metal bindingi311Calcium 2By similarity1
Metal bindingi311Calcium 3By similarity1
Metal bindingi314Calcium 3By similarity1
Metal bindingi317Calcium 2By similarity1
Metal bindingi317Calcium 3By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: GO_Central
  • calcium ion binding Source: GO_Central
  • clathrin binding Source: GO_Central
  • identical protein binding Source: IntAct
  • phosphatidylinositol-4,5-bisphosphate binding Source: ParkinsonsUK-UCL
  • phosphatidylserine binding Source: ParkinsonsUK-UCL
  • SNARE binding Source: ParkinsonsUK-UCL
  • syntaxin binding Source: GO_Central

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-9
Alternative name(s):
Synaptotagmin IX
Short name:
SytIX
Synaptotagmin V1 Publication
Gene namesi
Name:Syt9
Synonyms:Syt51 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1926373 Syt9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 52VesicularSequence analysisAdd BLAST52
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 491CytoplasmicSequence analysisAdd BLAST418

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839631 – 491Synaptotagmin-9Add BLAST491

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiQ9R0N9
PaxDbiQ9R0N9
PeptideAtlasiQ9R0N9
PRIDEiQ9R0N9

PTM databases

iPTMnetiQ9R0N9
PhosphoSitePlusiQ9R0N9

Expressioni

Gene expression databases

BgeeiENSMUSG00000062542 Expressed in 169 organ(s), highest expression level in habenula
CleanExiMM_SYT5
MM_SYT9
ExpressionAtlasiQ9R0N9 baseline and differential
GenevisibleiQ9R0N9 MM

Interactioni

Subunit structurei

Homodimer; disulfide-linked via the cysteine motif (PubMed:10531343). Can also form heterodimers with SYT3, SYT6, SYT7 and SYT10 (PubMed:10531343, PubMed:10531344, PubMed:10871604). Interacts with DNAJC5 and SNAP25, but not with HSC70 (PubMed:20847230). The interaction with DNAJC5 is stimulated tenfold in presence of calcium while the interaction with SNAP25 is inhibited (PubMed:20847230).4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9R0N9, 4 interactors
STRINGi10090.ENSMUSP00000073164

Structurei

3D structure databases

ProteinModelPortaliQ9R0N9
SMRiQ9R0N9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini222 – 323C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini354 – 457C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 31Cysteine motifBy similarityAdd BLAST23

Domaini

The cysteine motif mediates homo- or heterodimer formation via formation of disulfide bonds.By similarity

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1028 Eukaryota
ENOG410XQZ9 LUCA
GeneTreeiENSGT00760000118973
HOGENOMiHOG000232128
HOVERGENiHBG005010
InParanoidiQ9R0N9
KOiK19909
OMAiFNVAQFQ
OrthoDBiEOG091G0XMQ
TreeFamiTF315600

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR028691 SYT9
PANTHERiPTHR10024:SF180 PTHR10024:SF180, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9R0N9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGARDALCH QALQLLAELC ARGALEHDSC QDFIYHLRDR ARPRLRDPDI
60 70 80 90 100
SVSLLTLVVT ACGLALFGVS LFVSWKLCWV PWRERGLFSG SKDNNQEPLN
110 120 130 140 150
YTDTETNEQE NSEDFLDPPT PCPDSSMKIS HTSPDIPLST QPGGQENCAH
160 170 180 190 200
AVRVQRQVTE PTPSARHNSI RRQLNLSNPD FNIQQLQRQE QLTGIGRIKP
210 220 230 240 250
ELYKQRSLDN DDGRRSNSKA CGKLNFILKY DCDLEQLIVK IHKAVNLPAK
260 270 280 290 300
DFSGTSDPYV KIYLLPDRKT KHQTKVHRKT LNPVFDEVFL FPVHYNDLEA
310 320 330 340 350
RKLHFSVYDF DRFSRHDLIG QVVVDHFFDL ADFPRECILW KDIEYVTNDN
360 370 380 390 400
VDLGELMFSL CYLPTAGRLT ITIIKARNLK AMDITGASDP YVKVSLMCDG
410 420 430 440 450
RRLKKRKTST KRNTLNPVYN EAIVFDVPPE SIDQIHLSIA VMDYDRVGHN
460 470 480 490
EVIGVCQVGN EAERLGRDHW SEMLSYPRKP IAHWHSLMEK R
Length:491
Mass (Da):56,265
Last modified:July 27, 2011 - v2
Checksum:iE08ECFD9F4D6EE14
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RGS5D6RGS5_MOUSE
Synaptotagmin-9
Syt9
186Annotation score:
D3YVX0D3YVX0_MOUSE
Synaptotagmin-9
Syt9
69Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti488M → L in BAA85774 (PubMed:10531343).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026802 mRNA Translation: BAA85774.1
AK089115 mRNA Translation: BAC40759.1
AK156631 mRNA Translation: BAE33784.1
CH466531 Genomic DNA Translation: EDL16861.1
BC132495 mRNA Translation: AAI32496.1
BC137904 mRNA Translation: AAI37905.1
CCDSiCCDS40078.1
RefSeqiNP_068689.2, NM_021889.4
UniGeneiMm.302793

Genome annotation databases

EnsembliENSMUST00000073459; ENSMUSP00000073164; ENSMUSG00000062542
GeneIDi60510
KEGGimmu:60510
UCSCiuc009jba.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026802 mRNA Translation: BAA85774.1
AK089115 mRNA Translation: BAC40759.1
AK156631 mRNA Translation: BAE33784.1
CH466531 Genomic DNA Translation: EDL16861.1
BC132495 mRNA Translation: AAI32496.1
BC137904 mRNA Translation: AAI37905.1
CCDSiCCDS40078.1
RefSeqiNP_068689.2, NM_021889.4
UniGeneiMm.302793

3D structure databases

ProteinModelPortaliQ9R0N9
SMRiQ9R0N9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R0N9, 4 interactors
STRINGi10090.ENSMUSP00000073164

PTM databases

iPTMnetiQ9R0N9
PhosphoSitePlusiQ9R0N9

Proteomic databases

MaxQBiQ9R0N9
PaxDbiQ9R0N9
PeptideAtlasiQ9R0N9
PRIDEiQ9R0N9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073459; ENSMUSP00000073164; ENSMUSG00000062542
GeneIDi60510
KEGGimmu:60510
UCSCiuc009jba.1 mouse

Organism-specific databases

CTDi143425
MGIiMGI:1926373 Syt9

Phylogenomic databases

eggNOGiKOG1028 Eukaryota
ENOG410XQZ9 LUCA
GeneTreeiENSGT00760000118973
HOGENOMiHOG000232128
HOVERGENiHBG005010
InParanoidiQ9R0N9
KOiK19909
OMAiFNVAQFQ
OrthoDBiEOG091G0XMQ
TreeFamiTF315600

Enzyme and pathway databases

ReactomeiR-MMU-8856825 Cargo recognition for clathrin-mediated endocytosis
R-MMU-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

PROiPR:Q9R0N9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000062542 Expressed in 169 organ(s), highest expression level in habenula
CleanExiMM_SYT5
MM_SYT9
ExpressionAtlasiQ9R0N9 baseline and differential
GenevisibleiQ9R0N9 MM

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR028691 SYT9
PANTHERiPTHR10024:SF180 PTHR10024:SF180, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSYT9_MOUSE
AccessioniPrimary (citable) accession number: Q9R0N9
Secondary accession number(s): Q3U0R7, Q8C280
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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