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Protein

Synaptotagmin-6

Gene

Syt6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis (By similarity). May mediate Ca2+-regulation of exocytosis in acrosomal reaction in sperm (PubMed:15774481).By similarity1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi261Calcium 1By similarity1
Metal bindingi261Calcium 2By similarity1
Metal bindingi267Calcium 1By similarity1
Metal bindingi319Calcium 1By similarity1
Metal bindingi319Calcium 2By similarity1
Metal bindingi320Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi321Calcium 1By similarity1
Metal bindingi321Calcium 2By similarity1
Metal bindingi321Calcium 3By similarity1
Metal bindingi324Calcium 3By similarity1
Metal bindingi327Calcium 2By similarity1
Metal bindingi327Calcium 3By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: ParkinsonsUK-UCL
  • calcium-dependent protein binding Source: UniProtKB
  • calcium ion binding Source: GO_Central
  • clathrin binding Source: MGI
  • identical protein binding Source: IntAct
  • phosphatidylserine binding Source: ParkinsonsUK-UCL
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: BHF-UCL
  • SNARE binding Source: GO_Central
  • syntaxin binding Source: MGI

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-6
Alternative name(s):
Synaptotagmin VI
Short name:
SytVI
Gene namesi
Name:Syt6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1859544 Syt6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 59VesicularSequence analysisAdd BLAST59
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 511CytoplasmicSequence analysisAdd BLAST431

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839551 – 511Synaptotagmin-6Add BLAST511

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei217PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9R0N8
PRIDEiQ9R0N8

PTM databases

iPTMnetiQ9R0N8
PhosphoSitePlusiQ9R0N8

Expressioni

Tissue specificityi

Isoform 1 is expressed in the olfactory bulb. Isoform 2 is expressed in the brain (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000027849 Expressed in 77 organ(s), highest expression level in retina
CleanExiMM_SYT6
ExpressionAtlasiQ9R0N8 baseline and differential
GenevisibleiQ9R0N8 MM

Interactioni

Subunit structurei

Isoform 1: Homodimer; disulfide-linked via the cysteine motif (PubMed:10531343, PubMed:10531344). Isoform 1: Can also form heterodimers with SYT3, SYT7, SYT9 and SYT10 (PubMed:10531343, PubMed:10531344, PubMed:10871604). Isoform 1: Interacts with STX1A, STX1B and STX2; the interaction is Ca2+-dependent (PubMed:15774481). Isoform 2: Is not able to form homodimer and heterodimers (PubMed:10531344).4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9R0N8, 3 interactors
STRINGi10090.ENSMUSP00000088196

Structurei

3D structure databases

ProteinModelPortaliQ9R0N8
SMRiQ9R0N8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini232 – 333C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini364 – 467C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 38Cysteine motifBy similarityAdd BLAST27
Regioni483 – 511Necessary for cell membrane association (isoform 2)1 PublicationAdd BLAST29

Domaini

The cysteine motif mediates homo- or heterodimer formation via formation of disulfide bonds.By similarity

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IT3F Eukaryota
ENOG410YBMV LUCA
GeneTreeiENSGT00760000118973
HOGENOMiHOG000232128
HOVERGENiHBG005010
InParanoidiQ9R0N8
KOiK19906
OMAiTRHISFK
OrthoDBiEOG091G0XMQ
TreeFamiTF315600

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR028701 SYT6
PANTHERiPTHR10024:SF45 PTHR10024:SF45, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9R0N8-1) [UniParc]FASTAAdd to basket
Also known as: SytVI

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGVWGAGGP RCQAALAVLA SLCRARPPPL GLDVETCRSF ELQSPEQSPS
60 70 80 90 100
AADSGTSVSL LAVVVIVCGV ALVAVFLFLF WKLCWMPWRK KEASSPSSAN
110 120 130 140 150
PASETLQSPS SRGNMADKLK DPSALGFLEA AVKISHTSPD IPAEVQMSVK
160 170 180 190 200
EHIMRHTKLQ RQTTEPASST RHTSFKRHLP RQMHVSSVDY GNELPPAAAE
210 220 230 240 250
QPTSIGRIKP ELYKQKSVDG DDAKSEAAKS CGKINFSLRY DYESETLIVR
260 270 280 290 300
ILKAFDLPAK DFCGSSDPYV KIYLLPDRKC KLQTRVHRKT LNPTFDENFH
310 320 330 340 350
FPVPYEELAD RKLHLSVFDF DRFSRHDMIG EVILDNLFEA SDLSRETSIW
360 370 380 390 400
KDIQYATSES VDLGEIMFSL CYLPTAGRLT LTVIKCRNLK AMDITGYSDP
410 420 430 440 450
YVKVSLLCDG RRLKKKKTTI KKNTLNPIYN EAIIFDIPPE NMDQVSLLIS
460 470 480 490 500
VMDYDRVGHN EIIGVCRVGI NAEGLGRDHW NEMLAYPRKP IAHWHSLVEV
510
KKSFKEGTPR L
Length:511
Mass (Da):57,204
Last modified:July 27, 2011 - v2
Checksum:iC37C9305E3D05572
GO
Isoform 2 (identifier: Q9R0N8-2) [UniParc]FASTAAdd to basket
Also known as: SytVIdeltaTM1

The sequence of this isoform differs from the canonical sequence as follows:
     1-85: Missing.

Show »
Length:426
Mass (Da):48,343
Checksum:i699B22C7B06C3179
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UY13Q3UY13_MOUSE
Synaptotagmin-6
Syt6
342Annotation score:
Q8C8S6Q8C8S6_MOUSE
Synaptotagmin-6
Syt6
438Annotation score:
D6RIM4D6RIM4_MOUSE
Synaptotagmin-6
Syt6
93Annotation score:
V9GWX4V9GWX4_MOUSE
Synaptotagmin-6
Syt6
88Annotation score:
D6RHQ5D6RHQ5_MOUSE
Synaptotagmin-6
Syt6
71Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti428I → V in BAA85775 (PubMed:10531343).Curated1
Sequence conflicti428I → V in BAA85781 (PubMed:10531344).Curated1
Sequence conflicti428I → V in BAA85782 (PubMed:10531344).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0417291 – 85Missing in isoform 2. 1 PublicationAdd BLAST85

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026803 mRNA Translation: BAA85775.1
AB026809 mRNA Translation: BAA85781.1
AB026810 mRNA Translation: BAA85782.1
AC123057 Genomic DNA No translation available.
CCDSiCCDS38575.1 [Q9R0N8-1]
CCDS71296.1 [Q9R0N8-2]
RefSeqiNP_061270.2, NM_018800.4 [Q9R0N8-1]
UniGeneiMm.488213
Mm.88818

Genome annotation databases

EnsembliENSMUST00000090697; ENSMUSP00000088196; ENSMUSG00000027849 [Q9R0N8-1]
ENSMUST00000117221; ENSMUSP00000113373; ENSMUSG00000027849 [Q9R0N8-2]
ENSMUST00000121834; ENSMUSP00000112997; ENSMUSG00000027849 [Q9R0N8-1]
GeneIDi54524
KEGGimmu:54524
UCSCiuc008qsy.2 mouse [Q9R0N8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026803 mRNA Translation: BAA85775.1
AB026809 mRNA Translation: BAA85781.1
AB026810 mRNA Translation: BAA85782.1
AC123057 Genomic DNA No translation available.
CCDSiCCDS38575.1 [Q9R0N8-1]
CCDS71296.1 [Q9R0N8-2]
RefSeqiNP_061270.2, NM_018800.4 [Q9R0N8-1]
UniGeneiMm.488213
Mm.88818

3D structure databases

ProteinModelPortaliQ9R0N8
SMRiQ9R0N8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R0N8, 3 interactors
STRINGi10090.ENSMUSP00000088196

PTM databases

iPTMnetiQ9R0N8
PhosphoSitePlusiQ9R0N8

Proteomic databases

PaxDbiQ9R0N8
PRIDEiQ9R0N8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090697; ENSMUSP00000088196; ENSMUSG00000027849 [Q9R0N8-1]
ENSMUST00000117221; ENSMUSP00000113373; ENSMUSG00000027849 [Q9R0N8-2]
ENSMUST00000121834; ENSMUSP00000112997; ENSMUSG00000027849 [Q9R0N8-1]
GeneIDi54524
KEGGimmu:54524
UCSCiuc008qsy.2 mouse [Q9R0N8-1]

Organism-specific databases

CTDi148281
MGIiMGI:1859544 Syt6

Phylogenomic databases

eggNOGiENOG410IT3F Eukaryota
ENOG410YBMV LUCA
GeneTreeiENSGT00760000118973
HOGENOMiHOG000232128
HOVERGENiHBG005010
InParanoidiQ9R0N8
KOiK19906
OMAiTRHISFK
OrthoDBiEOG091G0XMQ
TreeFamiTF315600

Miscellaneous databases

ChiTaRSiSyt6 mouse
PROiPR:Q9R0N8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027849 Expressed in 77 organ(s), highest expression level in retina
CleanExiMM_SYT6
ExpressionAtlasiQ9R0N8 baseline and differential
GenevisibleiQ9R0N8 MM

Family and domain databases

Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR001565 Synaptotagmin
IPR028701 SYT6
PANTHERiPTHR10024:SF45 PTHR10024:SF45, 1 hit
PfamiView protein in Pfam
PF00168 C2, 2 hits
PRINTSiPR00360 C2DOMAIN
PR00399 SYNAPTOTAGMN
SMARTiView protein in SMART
SM00239 C2, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSYT6_MOUSE
AccessioniPrimary (citable) accession number: Q9R0N8
Secondary accession number(s): E9QJV2, Q9QUK7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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