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Protein

Podocalyxin

Gene

Podxl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.2 Publications

GO - Biological processi

Keywordsi

Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Podocalyxin
Alternative name(s):
Podocalyxin-like protein 1
Short name:
PC
Short name:
PCLP-1
Gene namesi
Name:Podxl
Synonyms:Pclp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1351317 Podxl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 404ExtracellularSequence analysisAdd BLAST383
Transmembranei405 – 425HelicalSequence analysisAdd BLAST21
Topological domaini426 – 503CytoplasmicSequence analysisAdd BLAST78

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

Die within the first 24 h of postnatal life from profound defects in kidney and/or gut formation. They are anuric (no measurable urine in the bladder), and fail to generate the extensive interdigitated foot process and instead retain cell junctions between immature podocytes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000002475522 – 503PodocalyxinAdd BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi94N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi154N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi206N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei463PhosphothreonineCombined sources1
Modified residuei482PhosphoserineBy similarity1
Modified residuei501PhosphothreonineBy similarity1

Post-translational modificationi

N- and O-linked glycosylated. Sialoglycoprotein (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9R0M4
PaxDbiQ9R0M4
PRIDEiQ9R0M4

PTM databases

iPTMnetiQ9R0M4
PhosphoSitePlusiQ9R0M4
SwissPalmiQ9R0M4

Expressioni

Tissue specificityi

Expressed in liver cells and hematopoietic cells (at protein level). Glomerular epithelium cell (podocyte).1 Publication

Gene expression databases

BgeeiENSMUSG00000025608 Expressed in 314 organ(s), highest expression level in metanephric S-shaped body
CleanExiMM_PODXL
ExpressionAtlasiQ9R0M4 baseline and differential
GenevisibleiQ9R0M4 MM

Interactioni

Subunit structurei

Monomer; when associated with the membrane raft. Oligomer; when integrated in the apical membrane. Found in a complex with EZR, PODXL and SLC9A3R2. Associates with the actin cytoskeleton through complex formation with EZR and SLC9A3R2. Interacts (via the C-terminal PDZ-binding motif DTHL) with SLC9A3R1 (via the PDZ domains); interaction is not detected in glomerular epithelium cells, take place early in the secretory pathway and is necessary for its apical membrane sorting. Interacts (via the C-terminal PDZ-binding motif DTHL) with SLC9A3R2 (via the PDZ 1 domain); interaction is detected in glomerular epithelium cells. Interacts with EZR (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9R0M4, 3 interactors
MINTiQ9R0M4
STRINGi10090.ENSMUSP00000026698

Structurei

3D structure databases

ProteinModelPortaliQ9R0M4
SMRiQ9R0M4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi27 – 259Ser/Thr-richAdd BLAST233

Domaini

Both the O-glycan-rich domain of the extracellular domain and the C-terminus PDZ-binding motif (DTHL) in the cytoplasmic tail harbor an apical sorting signal. The cytoplasmic domain is necessary for the apical membrane targeting and renal tubulogenesis. The large highly anionic extracellular domain allows to maintain open filtration pathways between neighboring podocyte foot processes. The cytoplasmic C-terminus PDZ-binding motif (DTHL) is essential for interaction with SLC9A3R1 and for targeting SLC9A3R1 to the apical cell membrane. The extracellular domain is necessary for microvillus formation (By similarity).By similarity

Sequence similaritiesi

Belongs to the podocalyxin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFKB Eukaryota
ENOG410YCG4 LUCA
GeneTreeiENSGT00730000111314
HOGENOMiHOG000115601
HOVERGENiHBG053629
InParanoidiQ9R0M4
KOiK06817
OMAiLFFHSGW
OrthoDBiEOG091G0RL9
PhylomeDBiQ9R0M4
TreeFamiTF333564

Family and domain databases

InterProiView protein in InterPro
IPR013836 CD34/Podocalyxin
IPR017403 PODXL
PANTHERiPTHR12067 PTHR12067, 1 hit
PfamiView protein in Pfam
PF06365 CD34_antigen, 1 hit
PIRSFiPIRSF038143 Podocalyxin-like_p1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R0M4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPPTTALSAL LLLLLSPASH SHNGNETSTS AIKSSTVQSH QSATTSTEVT
60 70 80 90 100
TGHPVASTLA STQPSNPTPF TTSTQSPSMP TSTPNPTSNQ SGGNLTSSVS
110 120 130 140 150
EVDKTKTSSP SSTAFTSSSG QTASSGGKSG DSFTTAPTTT LGLINVSSQP
160 170 180 190 200
TDLNTTSKLL STPTTDNTTS PQQPVDSSPS TASHPVGQHT PAAVPSSSGS
210 220 230 240 250
TPSTDNSTLT WKPTTHKPLG TSEATQPLTS QTPGITTLPV STLQQSMAST
260 270 280 290 300
VGTTTEEFTH LISNGTPVAP PGPSTPSPIW AFGNYQLNCE PPIRPDEELL
310 320 330 340 350
ILNLTRASLC ERSPLDEKEK LVELLCHSVK ASFKPAEDLC TLHVAPILDN
360 370 380 390 400
QAVAVKRIII ETKLSPKAVY ELLKDRWDDL TEAGVSDMKL GKEGPPEVNE
410 420 430 440 450
DRFSLPLIIT IVCMASFLLL VAALYGCCHQ RISQRKDQQR LTEELQTVEN
460 470 480 490 500
GYHDNPTLEV METPSEMQEK KVVNLNGELG DSWIVPLDNL TKDDLDEEED

THL
Length:503
Mass (Da):53,389
Last modified:November 16, 2001 - v2
Checksum:i786A1ECF65484D1F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti78S → F. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028048 mRNA Translation: BAA86912.1
AF290209 mRNA Translation: AAG02458.1
BC052442 mRNA Translation: AAH52442.1
BC054530 mRNA Translation: AAH54530.1
CCDSiCCDS19983.1
RefSeqiNP_038751.2, NM_013723.3
UniGeneiMm.89918

Genome annotation databases

EnsembliENSMUST00000026698; ENSMUSP00000026698; ENSMUSG00000025608
GeneIDi27205
KEGGimmu:27205
UCSCiuc009bgh.2 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPODXL_MOUSE
AccessioniPrimary (citable) accession number: Q9R0M4
Secondary accession number(s): Q9ESZ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 16, 2001
Last modified: September 12, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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