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Protein

A-kinase anchor protein 8-like

Gene

Akap8l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export. Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis. May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8. May be involved in regulation of pre-mRNA splicing (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri392 – 414C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri485 – 508C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 8-like
Short name:
AKAP8-like protein
Alternative name(s):
Neighbor of A-kinase-anchoring protein 95
Short name:
Neighbor of AKAP95
Gene namesi
Name:Akap8l
Synonyms:Nakap, Nakap95
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1860606 Akap8l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000753851 – 642A-kinase anchor protein 8-likeAdd BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei209Asymmetric dimethylarginine; alternateBy similarity1
Modified residuei209Omega-N-methylarginine; alternateBy similarity1
Modified residuei218Omega-N-methylarginineBy similarity1
Modified residuei238Omega-N-methylarginineBy similarity1
Modified residuei248Omega-N-methylarginineBy similarity1
Modified residuei258N6-acetyllysineBy similarity1
Modified residuei268PhosphothreonineBy similarity1
Modified residuei284PhosphoserineBy similarity1
Modified residuei293PhosphothreonineBy similarity1
Modified residuei298PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on serine or threonine residues possibly by PKA.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9R0L7
MaxQBiQ9R0L7
PaxDbiQ9R0L7
PeptideAtlasiQ9R0L7
PRIDEiQ9R0L7

PTM databases

iPTMnetiQ9R0L7
PhosphoSitePlusiQ9R0L7

Expressioni

Gene expression databases

CleanExiMM_AKAP8L

Interactioni

Subunit structurei

Interacts (via N-terminus) with DHX9 (via RGG region). Interacts with TMPO isoform Beta, PRPF40A, RNF43, lamin-B. Interacts with HDAC3; increased during mitosis (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9R0L7, 2 interactors
STRINGi10090.ENSMUSP00000051389

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 269Sufficient for activation of CTE-mediated expressionBy similarityAdd BLAST269

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi275 – 280Nuclear localization signalSequence analysis6
Motifi281 – 297Nuclear export signal (NES)By similarityAdd BLAST17
Motifi363 – 365Nuclear localization signalSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 52Gly/Tyr-richAdd BLAST12
Compositional biasi587 – 595Pro-rich9
Compositional biasi600 – 606Pro-rich7

Sequence similaritiesi

Belongs to the AKAP95 family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri392 – 414C2H2 AKAP95-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri485 – 508C2H2 AKAP95-type 2PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IIFX Eukaryota
ENOG410YCRZ LUCA
HOGENOMiHOG000169111
HOVERGENiHBG104297
InParanoidiQ9R0L7
PhylomeDBiQ9R0L7

Family and domain databases

InterProiView protein in InterPro
IPR007071 AKAP95
IPR034736 ZF_C2H2_AKAP95
PANTHERiPTHR12190 PTHR12190, 1 hit
PfamiView protein in Pfam
PF04988 AKAP95, 1 hit
PROSITEiView protein in PROSITE
PS51799 ZF_C2H2_AKAP95, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9R0L7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSYTGFVQGS ETTLQSTYCD TSAQPTCDYG YGTWNSGTNR GYENYGYGYG
60 70 80 90 100
YGQDNTTNYG YGMATSHSWE MASSDTNANP SASGSASADS VLSRINQRLD
110 120 130 140 150
MMPHLETDMI QGGVYGSGGG ERYDSYEACD SRAILSERDL YRSSYDYGEL
160 170 180 190 200
DPEMEMAYEG QYDAYRDQFR MRGGDTFGPR AQGWARDARS GRPMASGYGR
210 220 230 240 250
MWEDPMGARG QCMPGASRLP SLFSQNIIPE YGMFQGMRGG GAFSGGSRFG
260 270 280 290 300
FGFGNGMKQM RRTWKTWTTA DFRTKKKKRK QGGSPDEPDS KATRTDCSDN
310 320 330 340 350
SDSDNDEGTE GEAAEGTESA EAMEKGSRVD GEDEEGKEDG REEGKEDPEK
360 370 380 390 400
GALTAQDESS QAKRKLQASK KSQDKQKKRQ RDRMVERIQF VCSLCKYRTF
410 420 430 440 450
YEDEMGSHLD SKFHKEHFKY VGTKLPKQTA DFLQEYVTNK TKKTEELRKT
460 470 480 490 500
VEDLDGLIQQ IYRDQDLTQE IAMEHFVKKV EAAHCAACDL FIPMQFGIIQ
510 520 530 540 550
KHLKTMDHNR NRRLMMEQSK KSSLMVARSI LNNKLISKKL ERYLKGENPF
560 570 580 590 600
TDNPEEEKEQ DEVEAGALDE GAPSEATELT EGVPAQPPVP LEPAPGTTTP
610 620 630 640
PPPPPPEEEE SPVPLLGGAL QCQIRGIPGL DMEDDEEGGG GP
Length:642
Mass (Da):71,454
Last modified:May 1, 2000 - v1
Checksum:i4CE8DFE66810A0FC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5RL57Q5RL57_MOUSE
A kinase (PRKA) anchor protein 8-li...
Akap8l mCG_14258
641Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028921 mRNA Translation: BAA84711.1
UniGeneiMm.281005
Mm.488380

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028921 mRNA Translation: BAA84711.1
UniGeneiMm.281005
Mm.488380

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9R0L7, 2 interactors
STRINGi10090.ENSMUSP00000051389

PTM databases

iPTMnetiQ9R0L7
PhosphoSitePlusiQ9R0L7

Proteomic databases

EPDiQ9R0L7
MaxQBiQ9R0L7
PaxDbiQ9R0L7
PeptideAtlasiQ9R0L7
PRIDEiQ9R0L7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1860606 Akap8l

Phylogenomic databases

eggNOGiENOG410IIFX Eukaryota
ENOG410YCRZ LUCA
HOGENOMiHOG000169111
HOVERGENiHBG104297
InParanoidiQ9R0L7
PhylomeDBiQ9R0L7

Miscellaneous databases

PROiPR:Q9R0L7
SOURCEiSearch...

Gene expression databases

CleanExiMM_AKAP8L

Family and domain databases

InterProiView protein in InterPro
IPR007071 AKAP95
IPR034736 ZF_C2H2_AKAP95
PANTHERiPTHR12190 PTHR12190, 1 hit
PfamiView protein in Pfam
PF04988 AKAP95, 1 hit
PROSITEiView protein in PROSITE
PS51799 ZF_C2H2_AKAP95, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiAKP8L_MOUSE
AccessioniPrimary (citable) accession number: Q9R0L7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: July 18, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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