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Protein

Heat shock factor protein 4

Gene

Hsf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE). The HSF4A isoform represses transcription while the HSF4B isoform activates transcription.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi17 – 122By similarityAdd BLAST106

GO - Molecular functioni

  • DNA-binding transcription factor activity Source: GO_Central
  • protein phosphatase binding Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: GO_Central
  • sequence-specific DNA binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processStress response, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein 4
Short name:
HSF 4
Short name:
mHSF4
Alternative name(s):
Heat shock transcription factor 4
Short name:
HSTF 4
Gene namesi
Name:Hsf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1347058 Hsf4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001245721 – 492Heat shock factor protein 4Add BLAST492

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki293Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei298PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated mainly on serine residues. Phosphorylation on Ser-298 promotes sumoylation on Lys-293 (By similarity).By similarity
Isoform HSF4B is constitutively sumoylated. Sumoylation represses the transcriptional activity and is promoted by phosphorylation on Ser-298. HSFA is not sumoylated (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9R0L1
PRIDEiQ9R0L1

PTM databases

iPTMnetiQ9R0L1
PhosphoSitePlusiQ9R0L1

Expressioni

Tissue specificityi

Preferentially expressed in brain and lung. Also found in the eye. Slightly detected in liver and skeletal muscle. Isoform B is the major species in various tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000033249 Expressed in 124 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_HSF4
ExpressionAtlasiQ9R0L1 baseline and differential
GenevisibleiQ9R0L1 MM

Interactioni

Subunit structurei

Homotrimer. Exhibits constitutive DNA binding and forms trimers even in the absence of stress. Interacts with ALKBH4, DUSP26, MAPK1, MAPK2 and MAP kinase p38 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048904

Structurei

3D structure databases

ProteinModelPortaliQ9R0L1
SMRiQ9R0L1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 203Hydrophobic repeat HR-A/BAdd BLAST75
Regioni245 – 322Interactions with DUSP26, MAPK1 and MAPK2By similarityAdd BLAST78
Regioni364 – 389Hydrophobic repeat HR-CAdd BLAST26

Sequence similaritiesi

Belongs to the HSF family.Curated

Phylogenomic databases

eggNOGiKOG0627 Eukaryota
COG5169 LUCA
GeneTreeiENSGT00390000001182
HOGENOMiHOG000253917
HOVERGENiHBG005999
InParanoidiQ9R0L1
KOiK09417
OMAiMLIKEEP
OrthoDBiEOG091G087O
PhylomeDBiQ9R0L1
TreeFamiTF330401

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000232 HSF_DNA-bd
IPR027725 HSF_fam
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10015 PTHR10015, 1 hit
PfamiView protein in Pfam
PF00447 HSF_DNA-bind, 1 hit
PRINTSiPR00056 HSFDOMAIN
SMARTiView protein in SMART
SM00415 HSF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00434 HSF_DOMAIN, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform HSF4B (identifier: Q9R0L1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MQEAPAALPT EPGPSPVPAF LGKLWALVGD PGTDHLIRWS PSGTSFLVSD
60 70 80 90 100
QSRFAKEVLP QYFKHSNMAS FVRQLNMYGF RKVVSIEQGG LLRPERDHVE
110 120 130 140 150
FQHPSFVRGR EQLLERVRRK VPALRGDDSR WRPEDLSRLL GEVQALRGVQ
160 170 180 190 200
ESTEARLQEL RQQNEILWRE VVTLRQSHSQ QHRVIGKLIQ CLFGPLQTGP
210 220 230 240 250
SSTGAKRKLS LMLDEGSACS ASAKFNACPV SGALLQDPYF IQSPLPETTL
260 270 280 290 300
GLSPHRARGP IISDIPEDSP SPEGHRLSPS GGCRRVKGLA LLKEEPASPG
310 320 330 340 350
GDGEAGLALA PNECDFCVTA PPPLPVAVVQ AILEGKGSYS PEGPRSVQQP
360 370 380 390 400
EPRGPREVPD RGTLGLDRGN RSPESLLPPM LLRPAPETLE PVAPVDVLGP
410 420 430 440 450
SLHGREWTLM DLDMELSLMQ PLAPETDEAE LTVKELNSSG VGKDHTLGTP
460 470 480 490
LMLDVQADLE GAALSVPGAL TLYNVTESNA SYLDPGASPS SP
Length:492
Mass (Da):53,255
Last modified:February 21, 2001 - v2
Checksum:i952AB78255AD3CA0
GO
Isoform HSF4A (identifier: Q9R0L1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     245-319: LPETTLGLSP...APNECDFCVT → SPCSPSQRPR...TGFLPPVVAG

Show »
Length:462
Mass (Da):50,239
Checksum:iD2237C6323F1376D
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3UG93Q3UG93_MOUSE
Heat shock factor protein 4
Hsf4
432Annotation score:
G3UX32G3UX32_MOUSE
Heat shock factor protein 4
Hsf4
306Annotation score:
G3UYS8G3UYS8_MOUSE
Heat shock factor protein 4
Hsf4
332Annotation score:
G3UZF5G3UZF5_MOUSE
Heat shock factor protein 4
Hsf4
416Annotation score:
G3UXZ1G3UXZ1_MOUSE
Heat shock factor protein 4
Hsf4
47Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002419245 – 319LPETT…DFCVT → SPCSPSQRPRWASALTGPEG PSSLTSQKILHLLKDTGFLP PVVAG in isoform HSF4A. 1 PublicationAdd BLAST75

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029349 mRNA Translation: BAA84583.1
AB029350 mRNA Translation: BAA84584.1
CCDSiCCDS57638.1 [Q9R0L1-2]
CCDS57639.1 [Q9R0L1-1]
RefSeqiNP_001242971.1, NM_001256042.1 [Q9R0L1-1]
NP_036069.1, NM_011939.3 [Q9R0L1-2]
UniGeneiMm.89204

Genome annotation databases

EnsembliENSMUST00000036127; ENSMUSP00000048904; ENSMUSG00000033249 [Q9R0L1-1]
ENSMUST00000173859; ENSMUSP00000134213; ENSMUSG00000033249 [Q9R0L1-2]
GeneIDi26386
KEGGimmu:26386
UCSCiuc009nca.2 mouse [Q9R0L1-2]
uc009ncb.2 mouse [Q9R0L1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029349 mRNA Translation: BAA84583.1
AB029350 mRNA Translation: BAA84584.1
CCDSiCCDS57638.1 [Q9R0L1-2]
CCDS57639.1 [Q9R0L1-1]
RefSeqiNP_001242971.1, NM_001256042.1 [Q9R0L1-1]
NP_036069.1, NM_011939.3 [Q9R0L1-2]
UniGeneiMm.89204

3D structure databases

ProteinModelPortaliQ9R0L1
SMRiQ9R0L1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048904

PTM databases

iPTMnetiQ9R0L1
PhosphoSitePlusiQ9R0L1

Proteomic databases

PaxDbiQ9R0L1
PRIDEiQ9R0L1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036127; ENSMUSP00000048904; ENSMUSG00000033249 [Q9R0L1-1]
ENSMUST00000173859; ENSMUSP00000134213; ENSMUSG00000033249 [Q9R0L1-2]
GeneIDi26386
KEGGimmu:26386
UCSCiuc009nca.2 mouse [Q9R0L1-2]
uc009ncb.2 mouse [Q9R0L1-1]

Organism-specific databases

CTDi3299
MGIiMGI:1347058 Hsf4

Phylogenomic databases

eggNOGiKOG0627 Eukaryota
COG5169 LUCA
GeneTreeiENSGT00390000001182
HOGENOMiHOG000253917
HOVERGENiHBG005999
InParanoidiQ9R0L1
KOiK09417
OMAiMLIKEEP
OrthoDBiEOG091G087O
PhylomeDBiQ9R0L1
TreeFamiTF330401

Miscellaneous databases

PROiPR:Q9R0L1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033249 Expressed in 124 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_HSF4
ExpressionAtlasiQ9R0L1 baseline and differential
GenevisibleiQ9R0L1 MM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000232 HSF_DNA-bd
IPR027725 HSF_fam
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10015 PTHR10015, 1 hit
PfamiView protein in Pfam
PF00447 HSF_DNA-bind, 1 hit
PRINTSiPR00056 HSFDOMAIN
SMARTiView protein in SMART
SM00415 HSF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00434 HSF_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHSF4_MOUSE
AccessioniPrimary (citable) accession number: Q9R0L1
Secondary accession number(s): Q9R0L2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: November 7, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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