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Protein

Kunitz-type protease inhibitor 1

Gene

Spint1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitor of HGF activator.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei254 – 255Reactive bondBy similarity2
Sitei379 – 380Reactive bondBy similarity2

GO - Molecular functioni

GO - Biological processi

  • branching involved in labyrinthine layer morphogenesis Source: MGI
  • cellular response to BMP stimulus Source: Ensembl
  • embryonic placenta development Source: MGI
  • extracellular matrix organization Source: MGI
  • negative regulation of neural precursor cell proliferation Source: MGI
  • neural tube closure Source: MGI
  • placenta blood vessel development Source: MGI
  • positive regulation of glial cell differentiation Source: MGI

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-MMU-6806942 MET Receptor Activation

Protein family/group databases

MEROPSiI02.007

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type protease inhibitor 1
Alternative name(s):
Hepatocyte growth factor activator inhibitor type 1
Short name:
HAI-1
Gene namesi
Name:Spint1
Synonyms:Hai1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1338033 Spint1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001688430 – 507Kunitz-type protease inhibitor 1Add BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi229N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi244 ↔ 294By similarity
Disulfide bondi253 ↔ 277By similarity
Disulfide bondi269 ↔ 290By similarity
Disulfide bondi320 ↔ 338By similarity
Disulfide bondi332 ↔ 347By similarity
Disulfide bondi369 ↔ 419By similarity
Disulfide bondi378 ↔ 402By similarity
Disulfide bondi394 ↔ 415By similarity
Glycosylationi501N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9R097
PaxDbiQ9R097
PeptideAtlasiQ9R097
PRIDEiQ9R097

PTM databases

iPTMnetiQ9R097
PhosphoSitePlusiQ9R097

Expressioni

Gene expression databases

BgeeiENSMUSG00000027315
CleanExiMM_SPINT1
GenevisibleiQ9R097 MM

Interactioni

Subunit structurei

Interacts with HGFAC.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028783

Structurei

3D structure databases

ProteinModelPortaliQ9R097
SMRiQ9R097
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 134MANSCPROSITE-ProRule annotationAdd BLAST84
Domaini244 – 294BPTI/Kunitz inhibitor 1PROSITE-ProRule annotationAdd BLAST51
Domaini312 – 348LDL-receptor class APROSITE-ProRule annotationAdd BLAST37
Domaini369 – 419BPTI/Kunitz inhibitor 2PROSITE-ProRule annotationAdd BLAST51

Domaini

This inhibitor contains two inhibitory domains.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4295 Eukaryota
ENOG410XQNP LUCA
GeneTreeiENSGT00740000114929
HOGENOMiHOG000128104
HOVERGENiHBG061664
InParanoidiQ9R097
KOiK15619
OMAiECKLACR
OrthoDBiEOG091G0612
TreeFamiTF325867

Family and domain databases

CDDicd00109 KU, 2 hits
cd00112 LDLa, 1 hit
Gene3Di2.60.40.10, 1 hit
4.10.410.10, 2 hits
InterProiView protein in InterPro
IPR013783 Ig-like_fold
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR013980 MANSC_dom
IPR011106 MANSC_N
IPR020901 Prtase_inh_Kunz-CS
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 2 hits
PF00057 Ldl_recept_a, 1 hit
PF07502 MANEC, 1 hit
PRINTSiPR00759 BASICPTASE
SMARTiView protein in SMART
SM00131 KU, 2 hits
SM00192 LDLa, 1 hit
SM00765 MANEC, 1 hit
SUPFAMiSSF57362 SSF57362, 2 hits
SSF57424 SSF57424, 1 hit
PROSITEiView protein in PROSITE
PS00280 BPTI_KUNITZ_1, 2 hits
PS50279 BPTI_KUNITZ_2, 2 hits
PS01209 LDLRA_1, 1 hit
PS50068 LDLRA_2, 1 hit
PS50986 MANSC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9R097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRRLARAS ISAVGVWLLC ALGLQATEAE LPSAPAELPG GAACLSRFTS
60 70 80 90 100
GVPSFVLDTE ASVSNGATFL GSPTARRGWD CVRSCCTTQN CNLALVELQP
110 120 130 140 150
DGGEDAISAC FLMNCLYEQN FVCKFAPKEG FINYLTQELY RSYRELRTRG
160 170 180 190 200
FGGSRIPRIW MGIDLKVQLQ KPLVLNEADN TDWHLLQGDS DVRVERKRPE
210 220 230 240 250
EVELWGLKEG TYLFQLTRTD SDQPEETANL TITVLTAKQT EDYCLASYKV
260 270 280 290 300
GRCRGSFPRW YYDPKEQICK SFTFGGCLGN KNNYLREEEC MLACKDVQGI
310 320 330 340 350
SPKRHHPVCS GSCHATQFRC SNGCCIDGFL ECDDTPDCPD GSDEATCEKY
360 370 380 390 400
TSGFDELQNI HFLSDKGYCA ELPDTGFCKE NIPRWYYNPF SERCARFTYG
410 420 430 440 450
GCYGNKNNFE EEQQCLESCR GISKKDVFGL RREGSIPTVG SAEVAIAVFL
460 470 480 490 500
VICIIVVLTI LGYCFFKNQR KEFHSPLHHP PPTPASSTVS TTEDTEHLVY

NHTTQPL
Length:507
Mass (Da):56,591
Last modified:July 27, 2011 - v2
Checksum:iF7F9CCF2693D1F8D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102G → R in AAF02490 (PubMed:11406276).Curated1
Sequence conflicti238K → M in AAF02490 (PubMed:11406276).Curated1
Sequence conflicti325C → S in AAF02490 (PubMed:11406276).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099018 mRNA Translation: AAF02490.1
AL929318 Genomic DNA Translation: CAM25337.1
CH466519 Genomic DNA Translation: EDL27946.1
BC005769 mRNA Translation: AAH05769.1
CCDSiCCDS16597.1
RefSeqiNP_058603.2, NM_016907.3
XP_006499125.1, XM_006499062.3
UniGeneiMm.104955

Genome annotation databases

EnsembliENSMUST00000028783; ENSMUSP00000028783; ENSMUSG00000027315
ENSMUST00000110816; ENSMUSP00000106440; ENSMUSG00000027315
ENSMUST00000110817; ENSMUSP00000106441; ENSMUSG00000027315
GeneIDi20732
KEGGimmu:20732
UCSCiuc008ltk.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSPIT1_MOUSE
AccessioniPrimary (citable) accession number: Q9R097
Secondary accession number(s): Q99J04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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