UniProtKB - Q9R078 (AAKB1_MOUSE)
5'-AMP-activated protein kinase subunit beta-1
Prkab1
Functioni
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) (By similarity).
By similarityGO - Molecular functioni
- AMP-activated protein kinase activity Source: MGI
- protein kinase activity Source: MGI
- protein kinase binding Source: MGI
GO - Biological processi
- cellular response to nutrient levels Source: MGI
- fatty acid biosynthetic process Source: UniProtKB-KW
- nail development Source: MGI
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of gene expression Source: MGI
- protein phosphorylation Source: MGI
- regulation of catalytic activity Source: InterPro
- signal transduction Source: GO_Central
Keywordsi
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy R-MMU-380972, Energy dependent regulation of mTOR by LKB1-AMPK R-MMU-5628897, TP53 Regulates Metabolic Genes R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation |
Names & Taxonomyi
Protein namesi | Recommended name: 5'-AMP-activated protein kinase subunit beta-1Short name: AMPK subunit beta-1 Short name: AMPKb |
Gene namesi | Name:Prkab1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1336167, Prkab1 |
VEuPathDBi | HostDB:ENSMUSG00000029513 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: GO_Central
- nucleotide-activated protein kinase complex Source: MGI
- protein-containing complex Source: MGI
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000204364 | 2 – 270 | 5'-AMP-activated protein kinase subunit beta-1Add BLAST | 269 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 4 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 5 | PhosphoserineBy similarity | 1 | |
Modified residuei | 6 | PhosphoserineBy similarity | 1 | |
Modified residuei | 19 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 24 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Modified residuei | 25 | Phosphoserine; by autocatalysisBy similarity | 1 | |
Modified residuei | 40 | PhosphoserineBy similarity | 1 | |
Modified residuei | 96 | PhosphoserineCombined sources | 1 | |
Modified residuei | 101 | PhosphoserineBy similarity | 1 | |
Modified residuei | 108 | Phosphoserine; by autocatalysisCombined sources1 Publication | 1 | |
Modified residuei | 148 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 182 | PhosphoserineCombined sources | 1 | |
Modified residuei | 201 | N6-succinyllysineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
EPDi | Q9R078 |
jPOSTi | Q9R078 |
MaxQBi | Q9R078 |
PaxDbi | Q9R078 |
PRIDEi | Q9R078 |
ProteomicsDBi | 285894 |
PTM databases
CarbonylDBi | Q9R078 |
iPTMneti | Q9R078 |
PhosphoSitePlusi | Q9R078 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029513, Expressed in granulocyte and 312 other tissues |
Genevisiblei | Q9R078, MM |
Interactioni
Subunit structurei
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3).
Interacts with FNIP1 and FNIP2 (By similarity).
By similarityGO - Molecular functioni
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202363, 4 interactors |
ComplexPortali | CPX-5698, AMPK complex, alpha1-beta1-gamma1 variant CPX-5849, AMPK complex, alpha1-beta1-gamma2 variant CPX-5852, AMPK complex, alpha2-beta1-gamma1 variant CPX-5856, AMPK complex, alpha1-beta1-gamma3 variant CPX-5857, AMPK complex, alpha2-beta1-gamma3 variant CPX-5858, AMPK complex, alpha2-beta1-gamma2 variant |
CORUMi | Q9R078 |
IntActi | Q9R078, 7 interactors |
MINTi | Q9R078 |
STRINGi | 10090.ENSMUSP00000031486 |
Chemistry databases
BindingDBi | Q9R078 |
Miscellaneous databases
RNActi | Q9R078, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 44 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 68 – 163 | Glycogen-binding domainBy similarityAdd BLAST | 96 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 11 – 44 | Basic and acidic residuesSequence analysisAdd BLAST | 34 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1616, Eukaryota |
GeneTreei | ENSGT00940000155307 |
HOGENOMi | CLU_070949_2_0_1 |
InParanoidi | Q9R078 |
OMAi | NDRAPTQ |
OrthoDBi | 956412at2759 |
PhylomeDBi | Q9R078 |
TreeFami | TF313827 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR032640, AMPK1_CBM IPR039160, AMPKB IPR006828, ASC_dom IPR037256, ASC_dom_sf IPR013783, Ig-like_fold IPR014756, Ig_E-set |
PANTHERi | PTHR10343:SF89, PTHR10343:SF89, 1 hit |
Pfami | View protein in Pfam PF16561, AMPK1_CBM, 1 hit PF04739, AMPKBI, 1 hit |
SMARTi | View protein in SMART SM01010, AMPKBI, 1 hit |
SUPFAMi | SSF160219, SSF160219, 1 hit SSF81296, SSF81296, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGNTSSERAA LERQAGHKTP RRDSSGGAKD GDRPKILMDS PEDADIFHSE
60 70 80 90 100
EIKAPEKEEF LAWQHDLEAN DKAPAQARPT VFRWTGGGKE VYLSGSFNNW
110 120 130 140 150
SKLPLTRSQN NFVAILDLPE GEHQYKFFVD GQWTHDPSEP IVTSQLGTVN
160 170 180 190 200
NIIQVKKTDF EVFDALMVDS QKCSDVSELS SSPPGPYHQE PYMSKPEERF
210 220 230 240 250
KAPPILPPHL LQVILNKDTG ISCDPALLPE PNHVMLNHLY ALSIKDGVMV
260 270
LSATHRYKKK YVTTLLYKPI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketS4R2R1 | S4R2R1_MOUSE | 5'-AMP-activated protein kinase sub... | Prkab1 | 176 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF108215 mRNA Translation: AAF14222.1 BC016398 mRNA Translation: AAH16398.1 |
CCDSi | CCDS19598.1 |
RefSeqi | NP_114075.1, NM_031869.2 XP_006530264.1, XM_006530201.1 |
Genome annotation databases
Ensembli | ENSMUST00000031486; ENSMUSP00000031486; ENSMUSG00000029513 ENSMUST00000111999; ENSMUSP00000107630; ENSMUSG00000029513 |
GeneIDi | 19079 |
KEGGi | mmu:19079 |
UCSCi | uc008zew.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF108215 mRNA Translation: AAF14222.1 BC016398 mRNA Translation: AAH16398.1 |
CCDSi | CCDS19598.1 |
RefSeqi | NP_114075.1, NM_031869.2 XP_006530264.1, XM_006530201.1 |
3D structure databases
AlphaFoldDBi | Q9R078 |
SMRi | Q9R078 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202363, 4 interactors |
ComplexPortali | CPX-5698, AMPK complex, alpha1-beta1-gamma1 variant CPX-5849, AMPK complex, alpha1-beta1-gamma2 variant CPX-5852, AMPK complex, alpha2-beta1-gamma1 variant CPX-5856, AMPK complex, alpha1-beta1-gamma3 variant CPX-5857, AMPK complex, alpha2-beta1-gamma3 variant CPX-5858, AMPK complex, alpha2-beta1-gamma2 variant |
CORUMi | Q9R078 |
IntActi | Q9R078, 7 interactors |
MINTi | Q9R078 |
STRINGi | 10090.ENSMUSP00000031486 |
Chemistry databases
BindingDBi | Q9R078 |
ChEMBLi | CHEMBL4524004 |
PTM databases
CarbonylDBi | Q9R078 |
iPTMneti | Q9R078 |
PhosphoSitePlusi | Q9R078 |
Proteomic databases
EPDi | Q9R078 |
jPOSTi | Q9R078 |
MaxQBi | Q9R078 |
PaxDbi | Q9R078 |
PRIDEi | Q9R078 |
ProteomicsDBi | 285894 |
Protocols and materials databases
Antibodypediai | 1328, 829 antibodies from 42 providers |
DNASUi | 19079 |
Genome annotation databases
Ensembli | ENSMUST00000031486; ENSMUSP00000031486; ENSMUSG00000029513 ENSMUST00000111999; ENSMUSP00000107630; ENSMUSG00000029513 |
GeneIDi | 19079 |
KEGGi | mmu:19079 |
UCSCi | uc008zew.1, mouse |
Organism-specific databases
CTDi | 5564 |
MGIi | MGI:1336167, Prkab1 |
VEuPathDBi | HostDB:ENSMUSG00000029513 |
Phylogenomic databases
eggNOGi | KOG1616, Eukaryota |
GeneTreei | ENSGT00940000155307 |
HOGENOMi | CLU_070949_2_0_1 |
InParanoidi | Q9R078 |
OMAi | NDRAPTQ |
OrthoDBi | 956412at2759 |
PhylomeDBi | Q9R078 |
TreeFami | TF313827 |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy R-MMU-380972, Energy dependent regulation of mTOR by LKB1-AMPK R-MMU-5628897, TP53 Regulates Metabolic Genes R-MMU-6804756, Regulation of TP53 Activity through Phosphorylation |
Miscellaneous databases
BioGRID-ORCSi | 19079, 1 hit in 75 CRISPR screens |
ChiTaRSi | Prkab1, mouse |
PROi | PR:Q9R078 |
RNActi | Q9R078, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029513, Expressed in granulocyte and 312 other tissues |
Genevisiblei | Q9R078, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR032640, AMPK1_CBM IPR039160, AMPKB IPR006828, ASC_dom IPR037256, ASC_dom_sf IPR013783, Ig-like_fold IPR014756, Ig_E-set |
PANTHERi | PTHR10343:SF89, PTHR10343:SF89, 1 hit |
Pfami | View protein in Pfam PF16561, AMPK1_CBM, 1 hit PF04739, AMPKBI, 1 hit |
SMARTi | View protein in SMART SM01010, AMPKBI, 1 hit |
SUPFAMi | SSF160219, SSF160219, 1 hit SSF81296, SSF81296, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AAKB1_MOUSE | |
Accessioni | Q9R078Primary (citable) accession number: Q9R078 Secondary accession number(s): Q91YN6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families