UniProtKB - Q9R044 (NPHN_RAT)
Nephrin
Nphs1
Functioni
GO - Molecular functioni
- alpha-actinin binding Source: RGD
- cell adhesion molecule binding Source: GO_Central
- protein domain specific binding Source: RGD
- spectrin binding Source: RGD
GO - Biological processi
- cell adhesion Source: RGD
- cell-cell adhesion Source: GO_Central
- glomerular filtration Source: UniProtKB
- JNK cascade Source: RGD
- MAPK cascade Source: RGD
- myoblast fusion Source: RGD
- positive regulation of actin filament polymerization Source: RGD
- regulation of excretion Source: RGD
- skeletal muscle tissue development Source: RGD
Keywordsi
Molecular function | Developmental protein |
Biological process | Cell adhesion, Myogenesis |
Enzyme and pathway databases
Reactomei | R-RNO-373753, Nephrin family interactions |
Names & Taxonomyi
Protein namesi | Recommended name: NephrinAlternative name(s): Renal glomerulus-specific cell adhesion receptor |
Gene namesi | Name:Nphs1 Synonyms:Nphn |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620460, Nphs1 |
Subcellular locationi
Plasma membrane
- Cell membrane Curated; Single-pass type I membrane protein Curated
Note: Located at podocyte slit diaphragm between podocyte foot processes.1 Publication
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: UniProtKB
Other locations
- basement membrane Source: RGD
- cell projection Source: RGD
- cell-cell junction Source: GO_Central
- cytoplasm Source: RGD
- focal adhesion Source: RGD
- membrane Source: RGD
- membrane raft Source: RGD
- protein-containing complex Source: RGD
- slit diaphragm Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 36 – 1078 | ExtracellularSequence analysisAdd BLAST | 1043 | |
Transmembranei | 1079 – 1099 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1100 – 1252 | CytoplasmicSequence analysisAdd BLAST | 153 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 35 | Sequence analysisAdd BLAST | 35 | |
ChainiPRO_0000015054 | 36 – 1252 | NephrinAdd BLAST | 1217 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 54 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 67 ↔ 125 | PROSITE-ProRule annotation | ||
Disulfide bondi | 174 ↔ 231 | PROSITE-ProRule annotation | ||
Disulfide bondi | 279 ↔ 331 | PROSITE-ProRule annotation | ||
Glycosylationi | 370 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 375 ↔ 431 | PROSITE-ProRule annotation | ||
Modified residuei | 446 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 479 ↔ 542 | PROSITE-ProRule annotation | ||
Glycosylationi | 561 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 578 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 581 ↔ 637 | PROSITE-ProRule annotation | ||
Glycosylationi | 591 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 722 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 775 ↔ 830 | PROSITE-ProRule annotation | ||
Disulfide bondi | 877 ↔ 934 | PROSITE-ProRule annotation | ||
Modified residuei | 1112 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1115 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 1119 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1204 | Phosphotyrosine; by FYN1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q9R044 |
PRIDEi | Q9R044 |
PTM databases
GlyGeni | Q9R044, 6 sites |
iPTMneti | Q9R044 |
PhosphoSitePlusi | Q9R044 |
Expressioni
Tissue specificityi
Inductioni
Interactioni
Subunit structurei
Interacts with NPHS2 and with CD2AP (via C-terminal domain). Self-associates (via the Ig-like domains).
Also interacts (via the Ig-like domains) with KIRREL1/NEPH1 and KIRREL2; the interaction with KIRREL1 is dependent on KIRREL1 glycosylation.
Interacts with KIRREL3 (By similarity).
Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4.
Interacts with DDN; the interaction is direct.
Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1.
By similarity3 PublicationsBinary interactionsi
Q9R044
With | #Exp. | IntAct |
---|---|---|
Sirpa [P97710] | 2 | EBI-7945021,EBI-7945080 |
GO - Molecular functioni
- alpha-actinin binding Source: RGD
- cell adhesion molecule binding Source: GO_Central
- protein domain specific binding Source: RGD
- spectrin binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249144, 8 interactors |
CORUMi | Q9R044 |
IntActi | Q9R044, 1 interactor |
MINTi | Q9R044 |
STRINGi | 10116.ENSRNOP00000049922 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 144 | Ig-like C2-type 1Add BLAST | 106 | |
Domaini | 149 – 247 | Ig-like C2-type 2Add BLAST | 99 | |
Domaini | 256 – 347 | Ig-like C2-type 3Add BLAST | 92 | |
Domaini | 354 – 448 | Ig-like C2-type 4Add BLAST | 95 | |
Domaini | 454 – 554 | Ig-like C2-type 5Add BLAST | 101 | |
Domaini | 558 – 649 | Ig-like C2-type 6Add BLAST | 92 | |
Domaini | 754 – 846 | Ig-like C2-type 7Add BLAST | 93 | |
Domaini | 852 – 953 | Ig-like C2-type 8Add BLAST | 102 | |
Domaini | 957 – 1052 | Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST | 96 |
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3515, Eukaryota |
InParanoidi | Q9R044 |
OrthoDBi | 269917at2759 |
PhylomeDBi | Q9R044 |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 10 hits |
InterProi | View protein in InterPro IPR013162, CD80_C2-set IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 |
Pfami | View protein in Pfam PF08205, C2-set_2, 5 hits PF00041, fn3, 1 hit PF07679, I-set, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 1 hit SM00409, IG, 8 hits SM00408, IGc2, 7 hits |
SUPFAMi | SSF48726, SSF48726, 9 hits SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit PS50835, IG_LIKE, 7 hits |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGAKRVTVRG ARTSPIHRMS SLTPLLLMGM LTSGLAESPV PTSAPRGFWA
60 70 80 90 100
LSENLTAVEG TTVKLWCGVR APGSVVQWAK DGLLLGPNPK MPGFPRYSLE
110 120 130 140 150
GDRAKGEFHL LIEACDLSDD AEYECQVGRS ELGPELVSPK VILSILVSPK
160 170 180 190 200
VLLLTPEAGS TVTWVAGQEY VVTCVSGDAK PAPDITFIQS GRTILDVSSN
210 220 230 240 250
VNEGSEEKLC ITEAEARVIP QSSDNGQLLV CEGSNPALDT PIKASFTMNI
260 270 280 290 300
LFPPGPPVID WPGLNEGHVR AGENLELPCT ARGGNPPATL QWLKNGKPVS
310 320 330 340 350
TAWGTEHAQA VAHSVLVMTV RPEDHGARLS CQSYNSVSAG TQERSITLQV
360 370 380 390 400
TFPPSAITIL GSVSQSENKN VTLCCLTKSS RPRVLLRWWL GGRQLLPTDE
410 420 430 440 450
TVMDGLHGGH ISMSNLTFLV RREDNGLPLT CEAFSDAFSK ETFKKSLTLN
460 470 480 490 500
VKYPAQKLWI EGPPEGQYIR TGTRVRLVCL AIGGNPDPSL IWFKDSRPVS
510 520 530 540 550
EPRQPQEPRR VQLGSVEKSG STFSRELVLI IGPPDNRAKF SCKAGQLSAS
560 570 580 590 600
TQLVVQFPPT NLTILANSSA LRPGDALNLT CVSISSNPPV NLSWDKEGER
610 620 630 640 650
LEDVAAKPQS APFKGSAASR SVFLRVSSRD HGQRVTCRAH SEALRETVSS
660 670 680 690 700
FYRFNVLYPP EFLGEQVRAV TVVEQGQVLL PVSVSANPAP EAFNWTFRGY
710 720 730 740 750
RLSPAGGPRH RILSGGALQL WNVTRADDGF YQLHCQNSEG TAEALLKLDV
760 770 780 790 800
HYAPTIRALR DPTEVNVGGS VDIVCTVDAN PILPEMFSWE RLGEEEEDLN
810 820 830 840 850
LDDMEKVSKG STGRLRIRQA KLSQAGAYQC IVDNGVAPAA RGLVRLVVRF
860 870 880 890 900
APQVDQPTPL TKVAAAGDST SSATLHCRAR GVPNIDFTWT KNGVPLDLQD
910 920 930 940 950
PRYTEHRYHQ GVVHSSLLTI ANVSAAQDYA LFKCTATNAL GSDHTNIQLV
960 970 980 990 1000
SISRPDPPLG LKVVSISPHS VGLEWKPGFD GGLPQRFQIR YEALETPGFL
1010 1020 1030 1040 1050
HVDVLPTQAT TFTLTGLKPS TRYRIWLLAS NALGDSGLTD KGIQVSVTTP
1060 1070 1080 1090 1100
GPDQAPEDTD HQLPTELPPG PPRLPLLPVL FAVGGLLLLS NASCVGGLLW
1110 1120 1130 1140 1150
RRRLRRLAEE ISEKTEAGSE DRIRNEYEES QWTGDRDTRS STVSTAEVDP
1160 1170 1180 1190 1200
NYYSMRDFSP QLPPTLEEVL YHQGAEGEDM AFPGHLHDEV ERAYGPPGAW
1210 1220 1230 1240 1250
GPLYDEVRMD PYDLRWPEVQ CEDPRGIYDQ VAADMDAVEA SSLPFELRGH
LV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A140TAG4 | A0A140TAG4_RAT | Nephrin | Nphs1 | 1,252 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | M → T in AAF14884 (PubMed:10792613).Curated | 1 | |
Sequence conflicti | 46 | R → Q in AAF14884 (PubMed:10792613).Curated | 1 | |
Sequence conflicti | 1229 | D → N in AAF12734 (PubMed:10487848).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002600 | 1070 – 1155 | Missing in isoform 3. 1 PublicationAdd BLAST | 86 | |
Alternative sequenceiVSP_002599 | 1070 – 1082 | Missing in isoform 2. 1 PublicationAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF172255 mRNA Translation: AAF91086.1 AF161715 mRNA Translation: AAF14884.1 AF125521 mRNA Translation: AAF12734.1 |
RefSeqi | NP_072150.1, NM_022628.1 |
Genome annotation databases
GeneIDi | 64563 |
KEGGi | rno:64563 |
UCSCi | RGD:620460, rat [Q9R044-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF172255 mRNA Translation: AAF91086.1 AF161715 mRNA Translation: AAF14884.1 AF125521 mRNA Translation: AAF12734.1 |
RefSeqi | NP_072150.1, NM_022628.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 249144, 8 interactors |
CORUMi | Q9R044 |
IntActi | Q9R044, 1 interactor |
MINTi | Q9R044 |
STRINGi | 10116.ENSRNOP00000049922 |
PTM databases
GlyGeni | Q9R044, 6 sites |
iPTMneti | Q9R044 |
PhosphoSitePlusi | Q9R044 |
Proteomic databases
PaxDbi | Q9R044 |
PRIDEi | Q9R044 |
Genome annotation databases
GeneIDi | 64563 |
KEGGi | rno:64563 |
UCSCi | RGD:620460, rat [Q9R044-1] |
Organism-specific databases
CTDi | 4868 |
RGDi | 620460, Nphs1 |
Phylogenomic databases
eggNOGi | KOG3515, Eukaryota |
InParanoidi | Q9R044 |
OrthoDBi | 269917at2759 |
PhylomeDBi | Q9R044 |
Enzyme and pathway databases
Reactomei | R-RNO-373753, Nephrin family interactions |
Miscellaneous databases
PROi | PR:Q9R044 |
Family and domain databases
CDDi | cd00063, FN3, 1 hit |
Gene3Di | 2.60.40.10, 10 hits |
InterProi | View protein in InterPro IPR013162, CD80_C2-set IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 |
Pfami | View protein in Pfam PF08205, C2-set_2, 5 hits PF00041, fn3, 1 hit PF07679, I-set, 1 hit |
SMARTi | View protein in SMART SM00060, FN3, 1 hit SM00409, IG, 8 hits SM00408, IGc2, 7 hits |
SUPFAMi | SSF48726, SSF48726, 9 hits SSF49265, SSF49265, 1 hit |
PROSITEi | View protein in PROSITE PS50853, FN3, 1 hit PS50835, IG_LIKE, 7 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NPHN_RAT | |
Accessioni | Q9R044Primary (citable) accession number: Q9R044 Secondary accession number(s): Q9JIX2, Q9QXX7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2002 |
Last sequence update: | March 8, 2011 | |
Last modified: | April 7, 2021 | |
This is version 162 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families