UniProtKB - Q9QZX7 (SRR_MOUSE)
Protein
Serine racemase
Gene
Srr
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.5 Publications
Catalytic activityi
Cofactori
pyridoxal 5'-phosphate2 Publications
Activity regulationi
Allosterically activated by magnesium, and possibly also other divalent metal cations. Allosterically activated by ATP, ADP or GTP.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 51 | ATPCurated | 1 | |
Active sitei | 56 | Proton acceptorBy similarity | 1 | |
Active sitei | 84 | Proton acceptorBy similarity | 1 | |
Binding sitei | 121 | ATPBy similarity | 1 | |
Binding sitei | 135 | SubstrateBy similarity | 1 | |
Metal bindingi | 210 | MagnesiumBy similarity | 1 | |
Metal bindingi | 214 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 216 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: MGI
- calcium ion binding Source: MGI
- D-serine ammonia-lyase activity Source: UniProtKB-EC
- glycine binding Source: MGI
- identical protein binding Source: MGI
- L-serine ammonia-lyase activity Source: MGI
- magnesium ion binding Source: MGI
- PDZ domain binding Source: MGI
- protein homodimerization activity Source: MGI
- pyridoxal phosphate binding Source: MGI
- serine racemase activity Source: MGI
- threonine racemase activity Source: MGI
GO - Biological processi
- aging Source: Ensembl
- brain development Source: Ensembl
- D-serine biosynthetic process Source: MGI
- D-serine metabolic process Source: MGI
- L-serine metabolic process Source: MGI
- pyruvate biosynthetic process Source: MGI
- response to drug Source: Ensembl
- response to lipopolysaccharide Source: Ensembl
- response to morphine Source: Ensembl
- serine family amino acid metabolic process Source: MGI
Keywordsi
Molecular function | Allosteric enzyme, Isomerase, Lyase |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 4.3.1.17, 3474 5.1.1.18, 3474 |
Reactomei | R-MMU-977347, Serine biosynthesis |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Srr |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351636, Srr |
Subcellular locationi
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- apical part of cell Source: MGI
- cytoplasm Source: MGI
- neuronal cell body Source: MGI
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 51 | K → A: Impairs ATP-binding inducing a 80% decrease in enzyme activity. | 1 | |
Mutagenesisi | 113 | C → S: Abolishes S-nitrosylation. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL1075306 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185651 | 1 – 339 | Serine racemaseAdd BLAST | 339 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 56 | N6-(pyridoxal phosphate)lysineBy similarity | 1 | |
Modified residuei | 71 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 113 | S-nitrosocysteine1 Publication | 1 |
Post-translational modificationi
S-nitrosylated, leading to decrease the enzyme activity.1 Publication
Keywords - PTMi
Phosphoprotein, S-nitrosylationProteomic databases
jPOSTi | Q9QZX7 |
PaxDbi | Q9QZX7 |
PeptideAtlasi | Q9QZX7 |
PRIDEi | Q9QZX7 |
PTM databases
iPTMneti | Q9QZX7 |
PhosphoSitePlusi | Q9QZX7 |
SwissPalmi | Q9QZX7 |
Expressioni
Tissue specificityi
Detected in brain (at protein level). Brain.
Gene expression databases
Bgeei | ENSMUSG00000001323, Expressed in liver and 286 other tissues |
ExpressionAtlasi | Q9QZX7, baseline and differential |
Genevisiblei | Q9QZX7, MM |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
- PDZ domain binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 205172, 9 interactors |
IntActi | Q9QZX7, 2 interactors |
MINTi | Q9QZX7 |
STRINGi | 10090.ENSMUSP00000113372 |
Chemistry databases
BindingDBi | Q9QZX7 |
Miscellaneous databases
RNActi | Q9QZX7, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 238 – 239 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the serine/threonine dehydratase family.Curated
Phylogenomic databases
eggNOGi | KOG1251, Eukaryota |
GeneTreei | ENSGT00550000075026 |
HOGENOMi | CLU_021152_4_2_1 |
InParanoidi | Q9QZX7 |
OMAi | LIHPFDH |
OrthoDBi | 943371at2759 |
PhylomeDBi | Q9QZX7 |
TreeFami | TF313346 |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits |
InterProi | View protein in InterPro IPR001926, PLP-dep IPR000634, Ser/Thr_deHydtase_PyrdxlP-BS IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00291, PALP, 1 hit |
SUPFAMi | SSF53686, SSF53686, 1 hit |
PROSITEi | View protein in PROSITE PS00165, DEHYDRATASE_SER_THR, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9QZX7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MCAQYCISFA DVEKAHINIQ DSIHLTPVLT SSILNQIAGR NLFFKCELFQ
60 70 80 90 100
KTGSFKIRGA LNAIRGLIPD TPEEKPKAVV THSSGNHGQA LTYAAKLEGI
110 120 130 140 150
PAYIVVPQTA PNCKKLAIQA YGASIVYCDP SDESREKVTQ RIMQETEGIL
160 170 180 190 200
VHPNQEPAVI AGQGTIALEV LNQVPLVDAL VVPVGGGGMV AGIAITIKAL
210 220 230 240 250
KPSVKVYAAE PSNADDCYQS KLKGELTPNL HPPETIADGV KSSIGLNTWP
260 270 280 290 300
IIRDLVDDVF TVTEDEIKYA TQLVWGRMKL LIEPTAGVAL AAVLSQHFQT
310 320 330
VSPEVKNVCI VLSGGNVDLT SLNWVGQAER PAPYQTVSV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA8Y5D6 | A8Y5D6_MOUSE | L-serine ammonia-lyase | Srr | 113 | Annotation score: | ||
Z4YMF3 | Z4YMF3_MOUSE | L-serine ammonia-lyase | Srr | 166 | Annotation score: |
Sequence cautioni
The sequence CAI24255 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 111 | P → T in BAC25712 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_025013 | 174 – 198 | Missing in isoform 2. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_025014 | 183 – 339 | Missing in isoform 3. CuratedAdd BLAST | 157 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF148321 mRNA Translation: AAF08701.1 AK031687 mRNA Translation: BAC27514.1 AK028034 mRNA Translation: BAC25712.1 AK043738 mRNA Translation: BAC31637.1 AK157122 mRNA Translation: BAE33968.1 AK170096 mRNA Translation: BAE41561.1 AL604066 Genomic DNA Translation: CAI24252.1 AL604066 Genomic DNA Translation: CAI24253.1 AL604066 Genomic DNA Translation: CAI24254.1 AL604066 Genomic DNA Translation: CAI24255.1 Sequence problems. BC011164 mRNA Translation: AAH11164.1 AB232340 mRNA Translation: BAE19920.1 AB232341 mRNA Translation: BAE19921.1 AB232342 mRNA Translation: BAE19922.1 AB232343 mRNA Translation: BAE19923.1 AB235396 mRNA Translation: BAE44529.1 |
CCDSi | CCDS25038.1 [Q9QZX7-1] CCDS88190.1 [Q9QZX7-2] |
RefSeqi | NP_001156783.1, NM_001163311.1 [Q9QZX7-1] NP_038789.1, NM_013761.4 [Q9QZX7-1] XP_006533520.1, XM_006533457.3 [Q9QZX7-1] XP_017170058.1, XM_017314569.1 [Q9QZX7-1] XP_017170059.1, XM_017314570.1 XP_017170060.1, XM_017314571.1 [Q9QZX7-1] |
Genome annotation databases
Ensembli | ENSMUST00000065211; ENSMUSP00000067552; ENSMUSG00000001323 [Q9QZX7-1] ENSMUST00000108447; ENSMUSP00000104086; ENSMUSG00000001323 [Q9QZX7-2] ENSMUST00000108448; ENSMUSP00000104087; ENSMUSG00000001323 [Q9QZX7-1] ENSMUST00000121738; ENSMUSP00000113372; ENSMUSG00000001323 [Q9QZX7-1] ENSMUST00000128556; ENSMUSP00000120012; ENSMUSG00000001323 [Q9QZX7-3] |
GeneIDi | 27364 |
KEGGi | mmu:27364 |
UCSCi | uc007kcr.2, mouse [Q9QZX7-1] uc011xzd.1, mouse [Q9QZX7-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF148321 mRNA Translation: AAF08701.1 AK031687 mRNA Translation: BAC27514.1 AK028034 mRNA Translation: BAC25712.1 AK043738 mRNA Translation: BAC31637.1 AK157122 mRNA Translation: BAE33968.1 AK170096 mRNA Translation: BAE41561.1 AL604066 Genomic DNA Translation: CAI24252.1 AL604066 Genomic DNA Translation: CAI24253.1 AL604066 Genomic DNA Translation: CAI24254.1 AL604066 Genomic DNA Translation: CAI24255.1 Sequence problems. BC011164 mRNA Translation: AAH11164.1 AB232340 mRNA Translation: BAE19920.1 AB232341 mRNA Translation: BAE19921.1 AB232342 mRNA Translation: BAE19922.1 AB232343 mRNA Translation: BAE19923.1 AB235396 mRNA Translation: BAE44529.1 |
CCDSi | CCDS25038.1 [Q9QZX7-1] CCDS88190.1 [Q9QZX7-2] |
RefSeqi | NP_001156783.1, NM_001163311.1 [Q9QZX7-1] NP_038789.1, NM_013761.4 [Q9QZX7-1] XP_006533520.1, XM_006533457.3 [Q9QZX7-1] XP_017170058.1, XM_017314569.1 [Q9QZX7-1] XP_017170059.1, XM_017314570.1 XP_017170060.1, XM_017314571.1 [Q9QZX7-1] |
3D structure databases
SMRi | Q9QZX7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205172, 9 interactors |
IntActi | Q9QZX7, 2 interactors |
MINTi | Q9QZX7 |
STRINGi | 10090.ENSMUSP00000113372 |
Chemistry databases
BindingDBi | Q9QZX7 |
ChEMBLi | CHEMBL1075306 |
PTM databases
iPTMneti | Q9QZX7 |
PhosphoSitePlusi | Q9QZX7 |
SwissPalmi | Q9QZX7 |
Proteomic databases
jPOSTi | Q9QZX7 |
PaxDbi | Q9QZX7 |
PeptideAtlasi | Q9QZX7 |
PRIDEi | Q9QZX7 |
Protocols and materials databases
Antibodypediai | 4201, 298 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000065211; ENSMUSP00000067552; ENSMUSG00000001323 [Q9QZX7-1] ENSMUST00000108447; ENSMUSP00000104086; ENSMUSG00000001323 [Q9QZX7-2] ENSMUST00000108448; ENSMUSP00000104087; ENSMUSG00000001323 [Q9QZX7-1] ENSMUST00000121738; ENSMUSP00000113372; ENSMUSG00000001323 [Q9QZX7-1] ENSMUST00000128556; ENSMUSP00000120012; ENSMUSG00000001323 [Q9QZX7-3] |
GeneIDi | 27364 |
KEGGi | mmu:27364 |
UCSCi | uc007kcr.2, mouse [Q9QZX7-1] uc011xzd.1, mouse [Q9QZX7-2] |
Organism-specific databases
CTDi | 63826 |
MGIi | MGI:1351636, Srr |
Phylogenomic databases
eggNOGi | KOG1251, Eukaryota |
GeneTreei | ENSGT00550000075026 |
HOGENOMi | CLU_021152_4_2_1 |
InParanoidi | Q9QZX7 |
OMAi | LIHPFDH |
OrthoDBi | 943371at2759 |
PhylomeDBi | Q9QZX7 |
TreeFami | TF313346 |
Enzyme and pathway databases
BRENDAi | 4.3.1.17, 3474 5.1.1.18, 3474 |
Reactomei | R-MMU-977347, Serine biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 27364, 1 hit in 17 CRISPR screens |
PROi | PR:Q9QZX7 |
RNActi | Q9QZX7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001323, Expressed in liver and 286 other tissues |
ExpressionAtlasi | Q9QZX7, baseline and differential |
Genevisiblei | Q9QZX7, MM |
Family and domain databases
Gene3Di | 3.40.50.1100, 2 hits |
InterProi | View protein in InterPro IPR001926, PLP-dep IPR000634, Ser/Thr_deHydtase_PyrdxlP-BS IPR036052, Trypto_synt_PLP_dependent |
Pfami | View protein in Pfam PF00291, PALP, 1 hit |
SUPFAMi | SSF53686, SSF53686, 1 hit |
PROSITEi | View protein in PROSITE PS00165, DEHYDRATASE_SER_THR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SRR_MOUSE | |
Accessioni | Q9QZX7Primary (citable) accession number: Q9QZX7 Secondary accession number(s): Q401M7 Q8CD11 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 31, 2002 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families