UniProtKB - Q9QZQ8 (H2AY_MOUSE)
Core histone macro-H2A.1
Macroh2a1
Functioni
GO - Molecular functioni
- chromatin binding Source: MGI
- chromatin DNA binding Source: MGI
- DNA binding Source: GO_Central
- double-stranded methylated DNA binding Source: MGI
- enzyme binding Source: MGI
- nucleosomal DNA binding Source: UniProtKB
- promoter-specific chromatin binding Source: UniProtKB
- protein heterodimerization activity Source: InterPro
- protein kinase binding Source: MGI
- protein serine/threonine kinase inhibitor activity Source: MGI
- rDNA binding Source: MGI
- RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- chromatin organization Source: GO_Central
- chromatin silencing Source: GO_Central
- dosage compensation Source: MGI
- establishment of protein localization to chromatin Source: MGI
- negative regulation of cell cycle G2/M phase transition Source: MGI
- negative regulation of gene expression, epigenetic Source: MGI
- negative regulation of histone H3-K27 methylation Source: MGI
- negative regulation of histone H3-K4 methylation Source: MGI
- negative regulation of histone phosphorylation Source: MGI
- negative regulation of protein localization to chromosome, telomeric region Source: MGI
- negative regulation of protein serine/threonine kinase activity Source: MGI
- negative regulation of response to oxidative stress Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- negative regulation of transcription of nucleolar large rRNA by RNA polymerase I Source: UniProtKB
- nucleosome assembly Source: InterPro
- positive regulation of endodermal cell differentiation Source: MGI
- positive regulation of gene expression, epigenetic Source: MGI
- positive regulation of keratinocyte differentiation Source: MGI
- positive regulation of maintenance of mitotic sister chromatid cohesion Source: MGI
- positive regulation of response to oxidative stress Source: UniProtKB
- regulation of gene expression, epigenetic Source: MGI
- regulation of lipid metabolic process Source: MGI
- regulation of response to oxidative stress Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator, DNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Core histone macro-H2A.1Short name: Histone macroH2A1 Short name: mH2A1 Alternative name(s): H2A.y H2A/y |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1349392, Macroh2a1 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Other locations
- Chromosome 2 Publications
Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin (PubMed:9634239). In quiescent lymphocytes, associated with centromeric constitutive heterochromatin (PubMed:15564378).2 Publications
Cytoskeleton
- centrosome Source: MGI
Nucleus
- Barr body Source: MGI
- nuclear chromatin Source: MGI
- nuclear chromosome Source: MGI
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- chromatin Source: MGI
- condensed chromosome Source: MGI
- nucleosome Source: MGI
- pericentric heterochromatin Source: MGI
Keywords - Cellular componenti
Chromosome, Nucleosome core, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000227904 | 1 – 372 | Core histone macro-H2A.1Add BLAST | 372 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 18 | N6-methyllysineBy similarity | 1 | |
Modified residuei | 116 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 116 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Cross-linki | 117 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 123 | N6,N6-dimethyllysine; alternateBy similarity | 1 | |
Modified residuei | 123 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 123 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 129 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 167 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 170 | PhosphoserineCombined sources | 1 | |
Modified residuei | 173 | PhosphoserineCombined sources | 1 | |
Modified residuei | 178 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 189 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 323 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9QZQ8 |
jPOSTi | Q9QZQ8 |
PaxDbi | Q9QZQ8 |
PeptideAtlasi | Q9QZQ8 |
PRIDEi | Q9QZQ8 |
PTM databases
iPTMneti | Q9QZQ8 |
PhosphoSitePlusi | Q9QZQ8 |
SwissPalmi | Q9QZQ8 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000015937, Expressed in cochlea and 340 other tissues |
Genevisiblei | Q9QZQ8, MM |
Interactioni
Subunit structurei
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.
Interacts with SPOP.
Part of a complex consisting of MACROH2A1, CUL3 and SPOP.
Interacts with HDAC1 and HDAC2 (By similarity).
By similarityGO - Molecular functioni
- enzyme binding Source: MGI
- protein heterodimerization activity Source: InterPro
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 205061, 8 interactors |
IntActi | Q9QZQ8, 8 interactors |
MINTi | Q9QZQ8 |
STRINGi | 10090.ENSMUSP00000016081 |
Miscellaneous databases
RNActi | Q9QZQ8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 117 | Histone H2AAdd BLAST | 116 | |
Domaini | 184 – 370 | MacroPROSITE-ProRule annotationAdd BLAST | 187 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 116 – 161 | Lys-richAdd BLAST | 46 |
Phylogenomic databases
eggNOGi | KOG1756, Eukaryota KOG2633, Eukaryota |
GeneTreei | ENSGT00940000159541 |
HOGENOMi | CLU_062828_0_0_1 |
InParanoidi | Q9QZQ8 |
OMAi | AVIHPTN |
PhylomeDBi | Q9QZQ8 |
TreeFami | TF332276 |
Family and domain databases
CDDi | cd00074, H2A, 1 hit cd02904, Macro_H2A_like, 1 hit |
Gene3Di | 1.10.20.10, 1 hit 3.40.220.10, 1 hit |
InterProi | View protein in InterPro IPR021171, Core_histone_macro-H2A IPR009072, Histone-fold IPR002119, Histone_H2A IPR007125, Histone_H2A/H2B/H3 IPR032454, Histone_H2A_C IPR002589, Macro_dom IPR043472, Macro_dom-like IPR035796, Macro_H2A |
Pfami | View protein in Pfam PF00125, Histone, 1 hit PF16211, Histone_H2A_C, 1 hit PF01661, Macro, 1 hit |
PIRSFi | PIRSF037942, Core_histone_macro-H2A, 1 hit |
PRINTSi | PR00620, HISTONEH2A |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit SM00414, H2A, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51154, MACRO, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSRGGKKKS TKTSRSAKAG VIFPVGRMLR YIKKGHPKYR IGVGAPVYMA
60 70 80 90 100
AVLEYLTAEI LELAGNAARD NKKGRVTPRH ILLAVANDEE LNQLLKGVTI
110 120 130 140 150
ASGGVLPNIH PELLAKKRGS KGKLEAIITP PPAKKAKSPS QKKPVAKKTG
160 170 180 190 200
GKKGARKSKK KQGEVSKAAS ADSTTEGTPT DGFTVLSTKS LFLGQKLNLI
210 220 230 240 250
HSEISNLAGF EVEAIINPTN ADIDLKDDLG NTLEKKGGKE FVEAVLELRK
260 270 280 290 300
KNGPLEVAGA AISAGHGLPA KFVIHCNSPV WGADKCEELL EKTVKNCLAL
310 320 330 340 350
ADDRKLKSIA FPSIGSGRNG FPKQTAAQLI LKAISSYFVS TMSSSIKTVY
360 370
FMLFDSESIG IYVQEMAKLD AN
The sequence of this isoform differs from the canonical sequence as follows:
198-217: NLIHSEISNLAGFEVEAIIN → QVVQADIASIDSDAVVH
221-229: ADIDLKDDL → TDFYTGGEV
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017596 | 198 – 217 | NLIHS…EAIIN → QVVQADIASIDSDAVVH in isoform 1. 2 PublicationsAdd BLAST | 20 | |
Alternative sequenceiVSP_017597 | 221 – 229 | ADIDLKDDL → TDFYTGGEV in isoform 1. 2 Publications | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF171080 mRNA Translation: AAD53745.1 AF171081 mRNA Translation: AAD53746.1 AB071988 mRNA Translation: BAB68541.1 BC006955 mRNA Translation: AAH06955.1 |
CCDSi | CCDS26557.1 [Q9QZQ8-1] CCDS49278.1 [Q9QZQ8-2] |
RefSeqi | NP_001152985.1, NM_001159513.1 NP_001152986.1, NM_001159514.1 [Q9QZQ8-2] NP_001152987.1, NM_001159515.1 NP_036145.1, NM_012015.2 [Q9QZQ8-1] |
Genome annotation databases
Ensembli | ENSMUST00000016081; ENSMUSP00000016081; ENSMUSG00000015937 [Q9QZQ8-1] ENSMUST00000045788; ENSMUSP00000038221; ENSMUSG00000015937 [Q9QZQ8-2] |
GeneIDi | 26914 |
KEGGi | mmu:26914 |
UCSCi | uc007qsf.2, mouse [Q9QZQ8-1] uc007qsg.2, mouse [Q9QZQ8-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF171080 mRNA Translation: AAD53745.1 AF171081 mRNA Translation: AAD53746.1 AB071988 mRNA Translation: BAB68541.1 BC006955 mRNA Translation: AAH06955.1 |
CCDSi | CCDS26557.1 [Q9QZQ8-1] CCDS49278.1 [Q9QZQ8-2] |
RefSeqi | NP_001152985.1, NM_001159513.1 NP_001152986.1, NM_001159514.1 [Q9QZQ8-2] NP_001152987.1, NM_001159515.1 NP_036145.1, NM_012015.2 [Q9QZQ8-1] |
3D structure databases
SMRi | Q9QZQ8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205061, 8 interactors |
IntActi | Q9QZQ8, 8 interactors |
MINTi | Q9QZQ8 |
STRINGi | 10090.ENSMUSP00000016081 |
PTM databases
iPTMneti | Q9QZQ8 |
PhosphoSitePlusi | Q9QZQ8 |
SwissPalmi | Q9QZQ8 |
Proteomic databases
EPDi | Q9QZQ8 |
jPOSTi | Q9QZQ8 |
PaxDbi | Q9QZQ8 |
PeptideAtlasi | Q9QZQ8 |
PRIDEi | Q9QZQ8 |
Protocols and materials databases
Antibodypediai | 26391, 188 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000016081; ENSMUSP00000016081; ENSMUSG00000015937 [Q9QZQ8-1] ENSMUST00000045788; ENSMUSP00000038221; ENSMUSG00000015937 [Q9QZQ8-2] |
GeneIDi | 26914 |
KEGGi | mmu:26914 |
UCSCi | uc007qsf.2, mouse [Q9QZQ8-1] uc007qsg.2, mouse [Q9QZQ8-2] |
Organism-specific databases
CTDi | 9555 |
MGIi | MGI:1349392, Macroh2a1 |
Phylogenomic databases
eggNOGi | KOG1756, Eukaryota KOG2633, Eukaryota |
GeneTreei | ENSGT00940000159541 |
HOGENOMi | CLU_062828_0_0_1 |
InParanoidi | Q9QZQ8 |
OMAi | AVIHPTN |
PhylomeDBi | Q9QZQ8 |
TreeFami | TF332276 |
Miscellaneous databases
BioGRID-ORCSi | 26914, 1 hit in 18 CRISPR screens |
ChiTaRSi | H2afy, mouse |
PROi | PR:Q9QZQ8 |
RNActi | Q9QZQ8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000015937, Expressed in cochlea and 340 other tissues |
Genevisiblei | Q9QZQ8, MM |
Family and domain databases
CDDi | cd00074, H2A, 1 hit cd02904, Macro_H2A_like, 1 hit |
Gene3Di | 1.10.20.10, 1 hit 3.40.220.10, 1 hit |
InterProi | View protein in InterPro IPR021171, Core_histone_macro-H2A IPR009072, Histone-fold IPR002119, Histone_H2A IPR007125, Histone_H2A/H2B/H3 IPR032454, Histone_H2A_C IPR002589, Macro_dom IPR043472, Macro_dom-like IPR035796, Macro_H2A |
Pfami | View protein in Pfam PF00125, Histone, 1 hit PF16211, Histone_H2A_C, 1 hit PF01661, Macro, 1 hit |
PIRSFi | PIRSF037942, Core_histone_macro-H2A, 1 hit |
PRINTSi | PR00620, HISTONEH2A |
SMARTi | View protein in SMART SM00506, A1pp, 1 hit SM00414, H2A, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS51154, MACRO, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | H2AY_MOUSE | |
Accessioni | Q9QZQ8Primary (citable) accession number: Q9QZQ8 Secondary accession number(s): Q91VZ2, Q9QZQ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot