UniProtKB - Q9QZK2 (BCAR3_MOUSE)
Breast cancer anti-estrogen resistance protein 3 homolog
Bcar3
Functioni
Caution
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
- kinase binding Source: MGI
- phosphotyrosine residue binding Source: UniProtKB
GO - Biological processi
- endothelin receptor signaling pathway Source: UniProtKB
- insulin receptor signaling pathway Source: UniProtKB
- lens morphogenesis in camera-type eye Source: MGI
- positive regulation of DNA replication Source: UniProtKB
- positive regulation of epidermal growth factor receptor signaling pathway Source: UniProtKB
- positive regulation of GTPase activity Source: UniProtKB
- positive regulation of MAPK cascade Source: UniProtKB
- positive regulation of peptidyl-serine phosphorylation Source: MGI
- small GTPase mediated signal transduction Source: InterPro
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Names & Taxonomyi
Protein namesi | Recommended name: Breast cancer anti-estrogen resistance protein 3 homologAlternative name(s): p130Cas-binding protein AND-34 |
Gene namesi | Name:Bcar3 Synonyms:And34 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1352501, Bcar3 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
- focal adhesion 1 Publication
Note: Localization to focal adhesions depends on interaction with PTPRA.1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
- focal adhesion Source: UniProtKB
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cell junction, CytoplasmPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000230286 | 2 – 820 | Breast cancer anti-estrogen resistance protein 3 homologAdd BLAST | 819 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 32 | PhosphoserineBy similarity | 1 | |
Modified residuei | 72 | PhosphoserineBy similarity | 1 | |
Modified residuei | 77 | PhosphoserineBy similarity | 1 | |
Modified residuei | 176 | PhosphoserineBy similarity | 1 | |
Modified residuei | 284 | PhosphoserineBy similarity | 1 | |
Modified residuei | 329 | N6-methyllysineBy similarity | 1 | |
Modified residuei | 353 | PhosphoserineBy similarity | 1 | |
Modified residuei | 358 | PhosphoserineBy similarity | 1 | |
Modified residuei | 370 | PhosphoserineCombined sources | 1 | |
Modified residuei | 437 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 466 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
PaxDbi | Q9QZK2 |
PeptideAtlasi | Q9QZK2 |
PRIDEi | Q9QZK2 |
PTM databases
iPTMneti | Q9QZK2 |
PhosphoSitePlusi | Q9QZK2 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000028121, Expressed in primary oocyte and 282 other tissues |
Genevisiblei | Q9QZK2, MM |
Interactioni
Subunit structurei
Part of a complex comprised of PTPRA, BCAR1, BCAR3 (via SH2 domain) and SRC; the formation of the complex is dependent on integrin mediated-tyrosine phosphorylation of PTPRA (PubMed:22801373). Within the complex, interacts (via SH2 domain) with PTPRA (when phosphorylated on 'Tyr-825') (PubMed:22801373).
Interacts (via Ras-GEF domain) with BCAR1 (PubMed:10438950, PubMed:10896938, PubMed:12517963).
Interacts with (via Ras-GEF domain) NEDD9 (PubMed:12517963).
Interacts with PTK2B/FAK1 (PubMed:10896938).
Interacts with PTPN1.
Interacts (via SH2 domain) with EGFR (when tyrosine-phosphorylated) (By similarity).
By similarity4 PublicationsGO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
- kinase binding Source: MGI
- phosphotyrosine residue binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 205895, 1 interactor |
STRINGi | 10090.ENSMUSP00000029766 |
Miscellaneous databases
RNActi | Q9QZK2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 148 – 247 | SH2PROSITE-ProRule annotationAdd BLAST | 100 | |
Domaini | 543 – 813 | Ras-GEFPROSITE-ProRule annotationAdd BLAST | 271 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 739 – 743 | Mediates the interaction with BCAR1/p130CASBy similarity | 5 |
Domaini
Keywords - Domaini
SH2 domainPhylogenomic databases
eggNOGi | ENOG502QPX3, Eukaryota |
GeneTreei | ENSGT00940000154130 |
HOGENOMi | CLU_015281_0_0_1 |
InParanoidi | Q9QZK2 |
OMAi | CSAFHAF |
OrthoDBi | 138275at2759 |
PhylomeDBi | Q9QZK2 |
TreeFami | TF323756 |
Family and domain databases
CDDi | cd10337, SH2_BCAR3, 1 hit |
Gene3Di | 1.10.840.10, 1 hit 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR028849, BCAR3 IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR036964, RASGEF_cat_dom_sf IPR000980, SH2 IPR036860, SH2_dom_sf IPR044102, SH2_SHEP1/BCAR3/NSP1 |
PANTHERi | PTHR14247:SF10, PTHR14247:SF10, 1 hit |
Pfami | View protein in Pfam PF00617, RasGEF, 1 hit PF00017, SH2, 1 hit |
SMARTi | View protein in SMART SM00147, RasGEF, 1 hit SM00252, SH2, 1 hit |
SUPFAMi | SSF48366, SSF48366, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50009, RASGEF_CAT, 1 hit PS50001, SH2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAGKFASLP RNMPVNHQFP LASSMDLLSS KSPLAERRTD AYQDVSIHGT
60 70 80 90 100
LPRKKKGPPS IRSCDNAGHS KSPRQSSPLT QDIIQENPLQ DRKGENFIFR
110 120 130 140 150
DPYLLDPTLE YVKFSKERHI MDRTPERLKK ELEEELLLSS EDLRSHAWYH
160 170 180 190 200
GRIPRQVSEN LVQRDGDFLV RDSLSSPGNF VLTCQWKNLA QHFKINRTVL
210 220 230 240 250
RLSEAYSRVQ YQFEMESFDS IPGLVRCYVG NRRPISQQSG AIIFQPINRT
260 270 280 290 300
VPLWCLEERY GTSPGRGREG SLAEGRPDVV KRLSLTTGSS IQAREHSLPR
310 320 330 340 350
GNLLRNKEKS GSQPACLDHV QDRKALTLKA HQSESHLPIG CKLPPQSPSM
360 370 380 390 400
DTSPCPSSPV FRTGSEPTLS PALVRRFSSD ARTGEALRGS DSQLCPKPPP
410 420 430 440 450
KPCKVPFLKT PPSPSPWLTS EANYCELNPA FAVGCDRGAK LPMQAHDSHE
460 470 480 490 500
MLLTAKQNGP SGPRNSGINY MILDGDDQAR HWDPLAVQTD EGQEDKTKFV
510 520 530 540 550
PPLMETVSSF RPNDFESKLL PPENKPLETA MLKHAKELFT NHDARVIAQH
560 570 580 590 600
MLSVDCKVAR ILEVSEDRKR SMGVSSGLEL ITLPHGRQLR LDIIERHNTM
610 620 630 640 650
AIGIAVDILG CTGTLENRAG TLNKIIQVAV ELKDAMGDLY AFSAIMKALE
660 670 680 690 700
MPQITRLEKT WTALRHHYTQ TAILYEKQLK PFSKILHEGR ESTYVPASNV
710 720 730 740 750
SVPLLMPLVT LMERQAVTFE GTDMWENNDE SCEILLNHLA TARFMAEASE
760 770 780 790 800
SYRMNAERIL ADFQPDEEMT EILRTEFQMR LLWGSKGAEV NQNERYDKFN
810 820
QILTALSRKL EPPSGKQAEL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JGV2 | A0A0G2JGV2_MOUSE | Breast cancer anti-estrogen resista... | Bcar3 | 260 | Annotation score: | ||
A0A0G2JFC0 | A0A0G2JFC0_MOUSE | Breast cancer anti-estrogen resista... | Bcar3 | 186 | Annotation score: | ||
A0A0G2JDE7 | A0A0G2JDE7_MOUSE | Breast cancer anti-estrogen resista... | Bcar3 | 144 | Annotation score: | ||
A0A0G2JDR4 | A0A0G2JDR4_MOUSE | Breast cancer anti-estrogen resista... | Bcar3 | 178 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 | E → G in BAE25587 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 525 | K → E in BAE25587 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 795 | R → G in BAE38160 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_017815 | 1 – 120 | Missing in isoform 2. 1 PublicationAdd BLAST | 120 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF179566 mRNA Translation: AAD53182.1 AK143894 mRNA Translation: BAE25587.1 AK165396 mRNA Translation: BAE38160.1 BC023930 mRNA Translation: AAH23930.1 |
CCDSi | CCDS17810.1 [Q9QZK2-1] |
RefSeqi | NP_038895.1, NM_013867.2 [Q9QZK2-1] |
Genome annotation databases
Ensembli | ENSMUST00000029766; ENSMUSP00000029766; ENSMUSG00000028121 [Q9QZK2-1] |
GeneIDi | 29815 |
KEGGi | mmu:29815 |
UCSCi | uc008req.2, mouse [Q9QZK2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF179566 mRNA Translation: AAD53182.1 AK143894 mRNA Translation: BAE25587.1 AK165396 mRNA Translation: BAE38160.1 BC023930 mRNA Translation: AAH23930.1 |
CCDSi | CCDS17810.1 [Q9QZK2-1] |
RefSeqi | NP_038895.1, NM_013867.2 [Q9QZK2-1] |
3D structure databases
SMRi | Q9QZK2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205895, 1 interactor |
STRINGi | 10090.ENSMUSP00000029766 |
PTM databases
iPTMneti | Q9QZK2 |
PhosphoSitePlusi | Q9QZK2 |
Proteomic databases
PaxDbi | Q9QZK2 |
PeptideAtlasi | Q9QZK2 |
PRIDEi | Q9QZK2 |
Protocols and materials databases
Antibodypediai | 2973, 153 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000029766; ENSMUSP00000029766; ENSMUSG00000028121 [Q9QZK2-1] |
GeneIDi | 29815 |
KEGGi | mmu:29815 |
UCSCi | uc008req.2, mouse [Q9QZK2-1] |
Organism-specific databases
CTDi | 8412 |
MGIi | MGI:1352501, Bcar3 |
Phylogenomic databases
eggNOGi | ENOG502QPX3, Eukaryota |
GeneTreei | ENSGT00940000154130 |
HOGENOMi | CLU_015281_0_0_1 |
InParanoidi | Q9QZK2 |
OMAi | CSAFHAF |
OrthoDBi | 138275at2759 |
PhylomeDBi | Q9QZK2 |
TreeFami | TF323756 |
Miscellaneous databases
BioGRID-ORCSi | 29815, 0 hits in 17 CRISPR screens |
ChiTaRSi | Bcar3, mouse |
PROi | PR:Q9QZK2 |
RNActi | Q9QZK2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028121, Expressed in primary oocyte and 282 other tissues |
Genevisiblei | Q9QZK2, MM |
Family and domain databases
CDDi | cd10337, SH2_BCAR3, 1 hit |
Gene3Di | 1.10.840.10, 1 hit 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR028849, BCAR3 IPR023578, Ras_GEF_dom_sf IPR001895, RASGEF_cat_dom IPR036964, RASGEF_cat_dom_sf IPR000980, SH2 IPR036860, SH2_dom_sf IPR044102, SH2_SHEP1/BCAR3/NSP1 |
PANTHERi | PTHR14247:SF10, PTHR14247:SF10, 1 hit |
Pfami | View protein in Pfam PF00617, RasGEF, 1 hit PF00017, SH2, 1 hit |
SMARTi | View protein in SMART SM00147, RasGEF, 1 hit SM00252, SH2, 1 hit |
SUPFAMi | SSF48366, SSF48366, 1 hit SSF55550, SSF55550, 1 hit |
PROSITEi | View protein in PROSITE PS50009, RASGEF_CAT, 1 hit PS50001, SH2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BCAR3_MOUSE | |
Accessioni | Q9QZK2Primary (citable) accession number: Q9QZK2 Secondary accession number(s): Q3TNC9, Q3UP10 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 4, 2006 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot