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Protein

5'-AMP-activated protein kinase subunit beta-2

Gene

Prkab2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3) (By similarity).By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD
  • protein kinase binding Source: RGD

GO - Biological processi

Keywordsi

Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48

Names & Taxonomyi

Protein namesi
Recommended name:
5'-AMP-activated protein kinase subunit beta-2
Short name:
AMPK subunit beta-2
Gene namesi
Name:Prkab2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620905 Prkab2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002043701 – 2715'-AMP-activated protein kinase subunit beta-2Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38Phosphoserine; by ULK1Combined sources1 Publication1
Modified residuei39Phosphothreonine; by ULK11 Publication1
Modified residuei68Phosphoserine; by ULK11 Publication1
Modified residuei94PhosphoserineBy similarity1
Modified residuei107PhosphoserineBy similarity1
Modified residuei147PhosphothreonineBy similarity1
Modified residuei157PhosphoserineBy similarity1
Modified residuei169PhosphoserineBy similarity1
Modified residuei173Phosphoserine; by ULK11 Publication1
Modified residuei183Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QZH4
PRIDEiQ9QZH4

PTM databases

iPTMnetiQ9QZH4
PhosphoSitePlusiQ9QZH4

Interactioni

Subunit structurei

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062137

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9QZH4
SMRiQ9QZH4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1616 Eukaryota
ENOG410XRB3 LUCA
HOGENOMiHOG000230597
HOVERGENiHBG050430
InParanoidiQ9QZH4
KOiK07199
PhylomeDBiQ9QZH4

Family and domain databases

Gene3Di2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR032640 AMPK1_CBM
IPR039160 AMPKB
IPR006828 ASC_dom
IPR037256 ASC_dom_sf
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR10343:SF80 PTHR10343:SF80, 1 hit
PfamiView protein in Pfam
PF16561 AMPK1_CBM, 1 hit
PF04739 AMPKBI, 1 hit
SMARTiView protein in SMART
SM01010 AMPKBI, 1 hit
SUPFAMiSSF160219 SSF160219, 1 hit
SSF81296 SSF81296, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

Q9QZH4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGNTTSERVS GERHGAKAAR AEGGGHGPGK EHKIMVGSTD DPSVFSLPDS
60 70 80 90 100
KLPGDKEFVP WQQDLDDSVK PTQQARPTVI RWSEGGKEVF ISGSFNNWST
110 120 130 140 150
KIPLIKSHND FVAILDLPEG EHQYKFFVDG QWVHDPSEPV VTSQLGTINN
160 170 180 190 200
LIHVKKSDFE VFDALKLDSM ESSETSCRDL SSSPPGPYGQ EMYVFRSEER
210 220 230 240 250
FKSPPILPPH LLQVILNKDT NISCDPALLP EPNHVMLNHL YALSTKDSVM
260 270
VLSATHRYKK KYVTTLLYKP I
Length:271
Mass (Da):30,227
Last modified:May 1, 2000 - v1
Checksum:iD881451A03287BEA
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JVK3A0A0G2JVK3_RAT
5'-AMP-activated protein kinase sub...
Prkab2 rCG_51714
271Annotation score:
G3V9X3G3V9X3_RAT
5'-AMP-activated protein kinase sub...
Prkab2
179Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF182717 mRNA Translation: AAF01293.1
RefSeqiNP_072149.1, NM_022627.2
UniGeneiRn.207202

Genome annotation databases

GeneIDi64562
KEGGirno:64562

Similar proteinsi

Entry informationi

Entry nameiAAKB2_RAT
AccessioniPrimary (citable) accession number: Q9QZH4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 2000
Last modified: September 12, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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