UniProtKB - Q9QZH3 (PPIE_MOUSE)
Protein
Peptidyl-prolyl cis-trans isomerase E
Gene
Ppie
Organism
Mus musculus (Mouse)
Status
Functioni
Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.By similarity
Catalytic activityi
- EC:5.2.1.8By similarity
GO - Molecular functioni
- cyclosporin A binding Source: UniProtKB
- mRNA binding Source: UniProtKB
- peptidyl-prolyl cis-trans isomerase activity Source: UniProtKB
- poly(A) binding Source: UniProtKB
GO - Biological processi
- mRNA splicing, via spliceosome Source: UniProtKB
- positive regulation of viral genome replication Source: MGI
- protein folding Source: GO_Central
- protein peptidyl-prolyl isomerization Source: UniProtKB
- regulation of transcription, DNA-templated Source: MGI
Keywordsi
Molecular function | Isomerase, RNA-binding, Rotamase |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
Reactomei | R-MMU-6781823, Formation of TC-NER Pre-Incision Complex R-MMU-6782135, Dual incision in TC-NER R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-6798695, Neutrophil degranulation R-MMU-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Peptidyl-prolyl cis-trans isomerase E (EC:5.2.1.8By similarity)Short name: PPIase E Alternative name(s): Cyclophilin E Cyclophilin-33 Rotamase E |
Gene namesi | Name:Ppie Synonyms:Cyp331 Publication |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917118, Ppie |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- catalytic step 2 spliceosome Source: MGI
- nuclear speck Source: MGI
- nucleus Source: UniProtKB
- U2-type catalytic step 2 spliceosome Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064158 | 1 – 301 | Peptidyl-prolyl cis-trans isomerase EAdd BLAST | 301 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 91 | PhosphoserineBy similarity | 1 | |
Modified residuei | 97 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9QZH3 |
MaxQBi | Q9QZH3 |
PaxDbi | Q9QZH3 |
PeptideAtlasi | Q9QZH3 |
PRIDEi | Q9QZH3 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00111343 |
PTM databases
iPTMneti | Q9QZH3 |
PhosphoSitePlusi | Q9QZH3 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028651, Expressed in forelimb bud and 251 other tissues |
Genevisiblei | Q9QZH3, MM |
Interactioni
Subunit structurei
Identified in the spliceosome C complex.
Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE.
Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex. The IB complex does not contain PRPF19.
Interacts (via RNA-binding domain) with KMT2A (via the third PHD-type zinc-finger).
By similarityProtein-protein interaction databases
BioGRIDi | 207784, 19 interactors |
STRINGi | 10090.ENSMUSP00000030404 |
Miscellaneous databases
RNActi | Q9QZH3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 83 | RRMPROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 143 – 299 | PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST | 157 |
Domaini
The RRM domain mediates both interaction with RNA and with KMT2A (via the third PHD-type zinc-finger), but has much higher affinity for the KMT2A PHD-type zinc-finger.By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0111, Eukaryota |
GeneTreei | ENSGT00940000158790 |
HOGENOMi | CLU_012062_27_0_1 |
InParanoidi | Q9QZH3 |
OMAi | KIVIYAC |
OrthoDBi | 1484092at2759 |
PhylomeDBi | Q9QZH3 |
TreeFami | TF313817 |
Family and domain databases
CDDi | cd12347, RRM_PPIE, 1 hit |
Gene3Di | 2.40.100.10, 1 hit 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom IPR012677, Nucleotide-bd_a/b_plait_sf IPR016304, PPIE IPR034168, PPIE_RRM IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF001475, PPI_cyclophilin_E, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF50891, SSF50891, 1 hit SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit PS50102, RRM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9QZH3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATTKRVLYV GGLAEEVDDK VLHAAFIPFG DITDIQIPLD YETEKHRGFA
60 70 80 90 100
FVEFELAEDA AAAIDNMNES ELFGRTIRVN LAKPMRIKEG SSRPVWSDDD
110 120 130 140 150
WLKKFSGKTL EENKEEEGPE PPKAEAQEGE PTAKKARSNP QVYMDIKIGN
160 170 180 190 200
KPAGRIQMLL RSDVVPMTAE NFRCLCTHEK GFGFKGSSFH RIIPQFMCQG
210 220 230 240 250
GDFTNHNGTG GKSIYGKKFD DENFILKHTG PGLLSMANSG PNTNGSQFFL
260 270 280 290 300
TCDKTDWLDG KHVVFGEVTE GLDVLRQIEA QGSKDGKPKQ KVMIADCGEY
M
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 62 | A → P in AAF01030 (Ref. 3) Curated | 1 | |
Sequence conflicti | 241 | P → S in AAF01030 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK008177 mRNA Translation: BAB25512.1 BC045154 mRNA Translation: AAH45154.1 AF182825 mRNA Translation: AAF01030.1 |
CCDSi | CCDS18610.1 |
RefSeqi | NP_062362.1, NM_019489.5 |
Genome annotation databases
Ensembli | ENSMUST00000030404; ENSMUSP00000030404; ENSMUSG00000028651 |
GeneIDi | 56031 |
KEGGi | mmu:56031 |
UCSCi | uc008uow.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK008177 mRNA Translation: BAB25512.1 BC045154 mRNA Translation: AAH45154.1 AF182825 mRNA Translation: AAF01030.1 |
CCDSi | CCDS18610.1 |
RefSeqi | NP_062362.1, NM_019489.5 |
3D structure databases
BMRBi | Q9QZH3 |
SMRi | Q9QZH3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207784, 19 interactors |
STRINGi | 10090.ENSMUSP00000030404 |
PTM databases
iPTMneti | Q9QZH3 |
PhosphoSitePlusi | Q9QZH3 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00111343 |
Proteomic databases
EPDi | Q9QZH3 |
MaxQBi | Q9QZH3 |
PaxDbi | Q9QZH3 |
PeptideAtlasi | Q9QZH3 |
PRIDEi | Q9QZH3 |
Protocols and materials databases
Antibodypediai | 17854, 263 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000030404; ENSMUSP00000030404; ENSMUSG00000028651 |
GeneIDi | 56031 |
KEGGi | mmu:56031 |
UCSCi | uc008uow.1, mouse |
Organism-specific databases
CTDi | 10450 |
MGIi | MGI:1917118, Ppie |
Phylogenomic databases
eggNOGi | KOG0111, Eukaryota |
GeneTreei | ENSGT00940000158790 |
HOGENOMi | CLU_012062_27_0_1 |
InParanoidi | Q9QZH3 |
OMAi | KIVIYAC |
OrthoDBi | 1484092at2759 |
PhylomeDBi | Q9QZH3 |
TreeFami | TF313817 |
Enzyme and pathway databases
Reactomei | R-MMU-6781823, Formation of TC-NER Pre-Incision Complex R-MMU-6782135, Dual incision in TC-NER R-MMU-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-MMU-6798695, Neutrophil degranulation R-MMU-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 56031, 8 hits in 18 CRISPR screens |
ChiTaRSi | Ppie, mouse |
PROi | PR:Q9QZH3 |
RNActi | Q9QZH3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028651, Expressed in forelimb bud and 251 other tissues |
Genevisiblei | Q9QZH3, MM |
Family and domain databases
CDDi | cd12347, RRM_PPIE, 1 hit |
Gene3Di | 2.40.100.10, 1 hit 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR029000, Cyclophilin-like_dom_sf IPR020892, Cyclophilin-type_PPIase_CS IPR002130, Cyclophilin-type_PPIase_dom IPR012677, Nucleotide-bd_a/b_plait_sf IPR016304, PPIE IPR034168, PPIE_RRM IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00160, Pro_isomerase, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF001475, PPI_cyclophilin_E, 1 hit |
PRINTSi | PR00153, CSAPPISMRASE |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF50891, SSF50891, 1 hit SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS00170, CSA_PPIASE_1, 1 hit PS50072, CSA_PPIASE_2, 1 hit PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PPIE_MOUSE | |
Accessioni | Q9QZH3Primary (citable) accession number: Q9QZH3 Secondary accession number(s): Q9D8C0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | September 26, 2003 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families