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Protein

Eukaryotic translation initiation factor 3 subunit I

Gene

Eif3i

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit IUniRule annotation
Short name:
eIF3iUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 2UniRule annotation
TGF-beta receptor-interacting protein 1
Short name:
TRIP-1
eIF-3-betaUniRule annotation
eIF3 p36UniRule annotation
Gene namesi
Name:Eif3i
Synonyms:Eif3s2, Trip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1860763 Eif3i

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000510371 – 325Eukaryotic translation initiation factor 3 subunit IAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219PhosphothreonineBy similarity1
Modified residuei264N6-acetyllysineBy similarity1
Cross-linki282Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei308PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated by TGF-beta type II receptor.UniRule annotation

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9QZD9
MaxQBiQ9QZD9
PaxDbiQ9QZD9
PeptideAtlasiQ9QZD9
PRIDEiQ9QZD9

2D gel databases

REPRODUCTION-2DPAGEiQ9QZD9

PTM databases

iPTMnetiQ9QZD9
PhosphoSitePlusiQ9QZD9
SwissPalmiQ9QZD9

Expressioni

Gene expression databases

BgeeiENSMUSG00000028798 Expressed in 273 organ(s), highest expression level in floor plate of midbrain
ExpressionAtlasiQ9QZD9 baseline and differential
GenevisibleiQ9QZD9 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Eif4g1Q6NZJ63EBI-7466616,EBI-8175606

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207720, 56 interactors
IntActiQ9QZD9, 60 interactors
MINTiQ9QZD9
STRINGi10090.ENSMUSP00000099653

Structurei

3D structure databases

ProteinModelPortaliQ9QZD9
SMRiQ9QZD9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati8 – 47WD 1Add BLAST40
Repeati50 – 91WD 2Add BLAST42
Repeati144 – 183WD 3Add BLAST40
Repeati186 – 225WD 4Add BLAST40
Repeati283 – 324WD 5Add BLAST42

Sequence similaritiesi

Belongs to the eIF-3 subunit I family.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0643 Eukaryota
ENOG410XQ3E LUCA
GeneTreeiENSGT00630000089849
HOGENOMiHOG000231322
HOVERGENiHBG000900
InParanoidiQ9QZD9
KOiK03246
OMAiFETRFWH
OrthoDBiEOG091G0AIZ
PhylomeDBiQ9QZD9
TreeFamiTF101515

Family and domain databases

Gene3Di2.130.10.10, 1 hit
HAMAPiMF_03008 eIF3i, 1 hit
InterProiView protein in InterPro
IPR027525 eIF3i
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19877 PTHR19877, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 3 hits
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9QZD9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG
60 70 80 90 100
HTGAVWCVDA DWDTKHVLTG SADNSCRLWD CETGKQLALL KTNSAVRTCG
110 120 130 140 150
FDFGGNIIMF STDKQMGYQC FVSFFDLRDP SQIDSNEPYM KIPCNDSKIT
160 170 180 190 200
SAVWGPLGEC VIAGHESGEL NQYSAKSGEV LVNVKEHSRQ INDIQLSRDM
210 220 230 240 250
TMFVTASKDN TAKLFDSTTL EHQKTFRTER PVNSAALSPN YDHVVLGGGQ
260 270 280 290 300
EAMDVTTTST RIGKFEARFF HLAFEEEFGR VKGHFGPINS VAFHPDGKSY
310 320
SSGGEDGYVR IHYFDPQYFE FEFEA
Length:325
Mass (Da):36,461
Last modified:May 1, 2000 - v1
Checksum:i2B29405772675CC0
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2AE03A2AE03_MOUSE
Eukaryotic translation initiation f...
Eif3i
165Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF188297 mRNA Translation: AAF01455.1
AF271072 mRNA Translation: AAF76199.1
AK012375 mRNA Translation: BAB28197.1
AK002896 mRNA Translation: BAB22440.1
AK010781 mRNA Translation: BAB27177.1
BC029625 mRNA Translation: AAH29625.1
CCDSiCCDS18698.1
RefSeqiNP_061269.1, NM_018799.2
UniGeneiMm.250874

Genome annotation databases

EnsembliENSMUST00000102593; ENSMUSP00000099653; ENSMUSG00000028798
GeneIDi54709
KEGGimmu:54709
UCSCiuc008uxm.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF188297 mRNA Translation: AAF01455.1
AF271072 mRNA Translation: AAF76199.1
AK012375 mRNA Translation: BAB28197.1
AK002896 mRNA Translation: BAB22440.1
AK010781 mRNA Translation: BAB27177.1
BC029625 mRNA Translation: AAH29625.1
CCDSiCCDS18698.1
RefSeqiNP_061269.1, NM_018799.2
UniGeneiMm.250874

3D structure databases

ProteinModelPortaliQ9QZD9
SMRiQ9QZD9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207720, 56 interactors
IntActiQ9QZD9, 60 interactors
MINTiQ9QZD9
STRINGi10090.ENSMUSP00000099653

PTM databases

iPTMnetiQ9QZD9
PhosphoSitePlusiQ9QZD9
SwissPalmiQ9QZD9

2D gel databases

REPRODUCTION-2DPAGEiQ9QZD9

Proteomic databases

EPDiQ9QZD9
MaxQBiQ9QZD9
PaxDbiQ9QZD9
PeptideAtlasiQ9QZD9
PRIDEiQ9QZD9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102593; ENSMUSP00000099653; ENSMUSG00000028798
GeneIDi54709
KEGGimmu:54709
UCSCiuc008uxm.2 mouse

Organism-specific databases

CTDi8668
MGIiMGI:1860763 Eif3i

Phylogenomic databases

eggNOGiKOG0643 Eukaryota
ENOG410XQ3E LUCA
GeneTreeiENSGT00630000089849
HOGENOMiHOG000231322
HOVERGENiHBG000900
InParanoidiQ9QZD9
KOiK03246
OMAiFETRFWH
OrthoDBiEOG091G0AIZ
PhylomeDBiQ9QZD9
TreeFamiTF101515

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Miscellaneous databases

ChiTaRSiEif3i mouse
PROiPR:Q9QZD9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028798 Expressed in 273 organ(s), highest expression level in floor plate of midbrain
ExpressionAtlasiQ9QZD9 baseline and differential
GenevisibleiQ9QZD9 MM

Family and domain databases

Gene3Di2.130.10.10, 1 hit
HAMAPiMF_03008 eIF3i, 1 hit
InterProiView protein in InterPro
IPR027525 eIF3i
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19877 PTHR19877, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 3 hits
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3I_MOUSE
AccessioniPrimary (citable) accession number: Q9QZD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: May 1, 2000
Last modified: October 10, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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