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Protein

NFU1 iron-sulfur cluster scaffold homolog, mitochondrial

Gene

Nfu1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Iron-sulfur cluster scaffold protein which can assemble [4Fe-2S] clusters and deliver them to target proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi210Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1
Metal bindingi213Iron-sulfur (4Fe-4S); shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandIron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NFU1 iron-sulfur cluster scaffold homolog, mitochondrial
Alternative name(s):
HIRA-interacting protein 5
Short name:
mHIRIP5
Gene namesi
Name:Nfu1
Synonyms:Hirip5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1913290 Nfu1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionSequence analysisAdd BLAST29
ChainiPRO_000016619230 – 255NFU1 iron-sulfur cluster scaffold homolog, mitochondrialAdd BLAST226

Proteomic databases

EPDiQ9QZ23
MaxQBiQ9QZ23
PaxDbiQ9QZ23
PeptideAtlasiQ9QZ23
PRIDEiQ9QZ23

PTM databases

PhosphoSitePlusiQ9QZ23
SwissPalmiQ9QZ23

Expressioni

Gene expression databases

BgeeiENSMUSG00000029993 Expressed in 282 organ(s), highest expression level in brown adipose tissue
ExpressionAtlasiQ9QZ23 baseline and differential

Interactioni

Subunit structurei

Monomer and homohexamer; the apo-NFU1 is a monomer, while the holo-NFU1 is a hexamer composed of a trimer of dimer that is probably linked by some 4Fe-4S cluster. Interacts with HIRA and EPM2A/laforin. Interacts with BOLA3. Interacts with HSPA9.By similarity

Protein-protein interaction databases

IntActiQ9QZ23, 1 interactor
MINTiQ9QZ23
STRINGi10090.ENSMUSP00000032060

Structurei

Secondary structure

1255
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9QZ23
SMRiQ9QZ23
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QZ23

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni173 – 241NifUAdd BLAST69

Sequence similaritiesi

Belongs to the NifU family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2358 Eukaryota
COG0694 LUCA
GeneTreeiENSGT00390000011296
HOGENOMiHOG000023557
HOVERGENiHBG054438
InParanoidiQ9QZ23
KOiK22074
PhylomeDBiQ9QZ23
TreeFamiTF315076

Family and domain databases

Gene3Di3.30.1370.70, 1 hit
3.30.300.130, 1 hit
InterProiView protein in InterPro
IPR034904 FSCA_dom_sf
IPR014824 Nfu/NifU_N
IPR036498 Nfu/NifU_N_sf
IPR035433 NFU1-like
IPR001075 NIF_FeS_clus_asmbl_NifU_C
PfamiView protein in Pfam
PF08712 Nfu_N, 1 hit
PF01106 NifU, 1 hit
PIRSFiPIRSF036773 HIRIP5, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002830 NIF_FeS_clus_asmbl_NifU_C, 1 hit
SMARTiView protein in SMART
SM00932 Nfu_N, 1 hit
SUPFAMiSSF110836 SSF110836, 1 hit
SSF117916 SSF117916, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

Q9QZ23-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAAERAWGA AVGVVRLCRR FCHVATPHTF KKQPLHQYVR RPLFPLRAPL
60 70 80 90 100
CNTVRFMFIQ TQDTPNPNSL KFIPGKPVLE TRTMDFPTPA AAFRSPLARQ
110 120 130 140 150
LFRIEGVKSV FFGPDFITVT KENEELDWNL LKPDIYATIM DFFASGLPLV
160 170 180 190 200
TEETPPPPGE AGSEEDDEVV AMIKELLDTR IRPTVQEDGG DVIYRGFEDG
210 220 230 240 250
IVRLKLQGSC TSCPSSIITL KSGIQNMLQF YIPEVEGVEQ VMDDDESDEK

EANSS
Length:255
Mass (Da):28,568
Last modified:July 22, 2008 - v2
Checksum:iE7DA6A0333E2EB5E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0N4SUH8A0A0N4SUH8_MOUSE
NFU1 iron-sulfur cluster scaffold h...
Nfu1
256Annotation score:
D3Z285D3Z285_MOUSE
NFU1 iron-sulfur cluster scaffold h...
Nfu1
255Annotation score:

Sequence cautioni

The sequence AAH18355 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC24985 differs from that shown. Reason: Frameshift at positions 33 and 48.Curated
The sequence CAB57314 differs from that shown. Reason: Frameshift at positions 5, 8 and 50.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11 – 12AV → TS in AAQ73785 (PubMed:12915448).Curated2
Sequence conflicti19R → K in CAB57314 (PubMed:11342215).Curated1
Sequence conflicti39V → A in CAB57314 (PubMed:11342215).Curated1
Sequence conflicti39V → A in AAH18355 (PubMed:15489334).Curated1
Sequence conflicti39V → A in AAQ73785 (PubMed:12915448).Curated1
Sequence conflicti48A → H in CAB57314 (PubMed:11342215).Curated1
Sequence conflicti163S → SS in BAC24985 (PubMed:16141072).Curated1
Sequence conflicti163S → SS in BAE26508 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002244 mRNA Translation: BAC24985.1 Frameshift.
AK003730 mRNA Translation: BAB22965.1
AK145558 mRNA Translation: BAE26508.1
AJ132616 mRNA Translation: CAB57314.1 Frameshift.
BC018355 mRNA Translation: AAH18355.1 Different initiation.
AY335195 mRNA Translation: AAQ73785.1
CCDSiCCDS85088.1
RefSeqiNP_001164062.1, NM_001170591.1
NP_064429.2, NM_020045.3
XP_006506482.1, XM_006506419.1
XP_006506483.1, XM_006506420.1
UniGeneiMm.23809

Genome annotation databases

EnsembliENSMUST00000117583; ENSMUSP00000113332; ENSMUSG00000029993
GeneIDi56748
KEGGimmu:56748
UCSCiuc009csv.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNFU1_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ23
Secondary accession number(s): Q3ULE0
, Q6VNZ7, Q8CF80, Q9D1B7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: July 22, 2008
Last modified: September 12, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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