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Protein

C-type lectin domain family 4 member A

Gene

Clec4a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in regulating immune reactivity. May play a role in modulating dendritic cells (DC) differentiation and/or maturation (By similarity). May be involved in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation.By similarity1 Publication
C-type lectin receptor that binds carbohydrates mannose and fucose but also weakly interacts with N-acetylglucosamine (GlcNAc) in a Ca2+-dependent manner. Involved in regulating immune reactivity. Once triggered by antigen, it is internalized by clathrin-dependent endocytosis and delivers its antigenic cargo into the antigen presentation pathway resulting in cross-priming of CD8+ T cells. This cross-presentation and cross-priming are enhanced by TLR7 and TLR8 agonists with increased expansion of the CD8+ T cells, high production of IFNG and TNF with reduced levels of IL4, IL5 and IL13. In plasmacytoid dendritic cells, inhibits TLR9-mediated IFNA and TNF production (By similarity). May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation (PubMed:11841542).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi146Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi152Calcium 1By similarity1
Metal bindingi197Calcium 2By similarity1
Metal bindingi199Calcium 2By similarity1
Metal bindingi203Calcium 2By similarity1
Binding sitei203MannoseBy similarity1
Metal bindingi219Calcium 2By similarity1
Metal bindingi220Calcium 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity, Innate immunity
LigandCalcium, Lectin, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
C-type lectin domain family 4 member A
Alternative name(s):
C-type lectin superfamily member 6
Dendritic cell immunoreceptor
CD_antigen: CD367
Gene namesi
Name:Clec4a
Synonyms:Clec4a2, Clecsf6, Dcir
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1349412 Clec4a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicSequence analysisAdd BLAST48
Transmembranei49 – 69Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini70 – 238ExtracellularSequence analysisAdd BLAST169

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7Y → F: Loss of inhibition of B-cell calcium mobilization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000466131 – 238C-type lectin domain family 4 member AAdd BLAST238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi91N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi107 ↔ 118PROSITE-ProRule annotation
Glycosylationi131N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi136N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi137 ↔ 231PROSITE-ProRule annotation
Disulfide bondi205 ↔ 223PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9QZ15
PRIDEiQ9QZ15

PTM databases

iPTMnetiQ9QZ15
PhosphoSitePlusiQ9QZ15

Expressioni

Tissue specificityi

Expressed in splenic antigen-presenting cells including B-cells, monocytes/macrophages, and dendritic cells (at protein level). Expressed in spleen and lymph node and slightly increased with dendritic cell maturation.1 Publication

Gene expression databases

BgeeiENSMUSG00000030148 Expressed in 111 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_CLEC4A2
ExpressionAtlasiQ9QZ15 baseline and differential
GenevisibleiQ9QZ15 MM

Interactioni

Subunit structurei

May interact with PTPN6 via its ITIM site.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9QZ15
SMRiQ9QZ15
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 233C-type lectinPROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 199Mannose bindingBy similarity3
Regioni209 – 211N-acetyl-D-glucosamine bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi5 – 10ITIM motif6

Domaini

Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.1 Publication

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00930000150891
HOGENOMiHOG000059556
HOVERGENiHBG050992
InParanoidiQ9QZ15
KOiK10057
OMAiAPHKSNT
PhylomeDBiQ9QZ15

Family and domain databases

CDDicd03590 CLECT_DC-SIGN_like, 1 hit
Gene3Di3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR018378 C-type_lectin_CS
IPR033989 CD209-like_CTLD
IPR016187 CTDL_fold
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
SMARTiView protein in SMART
SM00034 CLECT, 1 hit
SUPFAMiSSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS00615 C_TYPE_LECTIN_1, 1 hit
PS50041 C_TYPE_LECTIN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9QZ15-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASEITYAEV KFKNESNSLH TYSESPAAPR EKPIRDLRKP GSPSLLLTSL
60 70 80 90 100
MLLLLLLAIT FLVAFIIYFQ KYSQLLEEKK AAKNIMHNEL NCTKSVSPME
110 120 130 140 150
DKVWSCCPKD WRLFGSHCYL VPTVSSSASW NKSEENCSRM GAHLVVIQSQ
160 170 180 190 200
EEQDFITGIL DTHAAYFIGL WDTGHRQWQW VDQTPYEESI TFWHNGEPSS
210 220 230
GNEKCATIIY RWKTGWGWND ISCSLKQKSV CQMKKINL
Length:238
Mass (Da):27,323
Last modified:May 1, 2000 - v1
Checksum:i2DB8AE0E11B18A56
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q923C7Q923C7_MOUSE
C-type lectin domain family 4, memb...
Clec4a2 Clecsf6, mCG_141420
262Annotation score:
E0CYQ6E0CYQ6_MOUSE
C-type lectin domain family 4, memb...
Clec4a2
74Annotation score:
E0CY25E0CY25_MOUSE
C-type lectin domain family 4, memb...
Clec4a2
27Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133533 mRNA Translation: CAB57870.1
AF387099 mRNA Translation: AAM22402.1
AK049002 mRNA Translation: BAC33509.1
BC075729 mRNA Translation: AAH75729.1
BC096565 mRNA Translation: AAH96565.1
CCDSiCCDS20510.1
RefSeqiNP_001163803.1, NM_001170332.1
NP_001163804.1, NM_001170333.1
NP_036129.1, NM_011999.4
UniGeneiMm.47384

Genome annotation databases

EnsembliENSMUST00000041779; ENSMUSP00000045781; ENSMUSG00000030148
ENSMUST00000161365; ENSMUSP00000124615; ENSMUSG00000030148
GeneIDi26888
KEGGimmu:26888
UCSCiuc009dqa.1 mouse

Similar proteinsi

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

DCIR1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133533 mRNA Translation: CAB57870.1
AF387099 mRNA Translation: AAM22402.1
AK049002 mRNA Translation: BAC33509.1
BC075729 mRNA Translation: AAH75729.1
BC096565 mRNA Translation: AAH96565.1
CCDSiCCDS20510.1
RefSeqiNP_001163803.1, NM_001170332.1
NP_001163804.1, NM_001170333.1
NP_036129.1, NM_011999.4
UniGeneiMm.47384

3D structure databases

ProteinModelPortaliQ9QZ15
SMRiQ9QZ15
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ9QZ15
PhosphoSitePlusiQ9QZ15

Proteomic databases

MaxQBiQ9QZ15
PRIDEiQ9QZ15

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041779; ENSMUSP00000045781; ENSMUSG00000030148
ENSMUST00000161365; ENSMUSP00000124615; ENSMUSG00000030148
GeneIDi26888
KEGGimmu:26888
UCSCiuc009dqa.1 mouse

Organism-specific databases

CTDi26888
MGIiMGI:1349412 Clec4a2

Phylogenomic databases

GeneTreeiENSGT00930000150891
HOGENOMiHOG000059556
HOVERGENiHBG050992
InParanoidiQ9QZ15
KOiK10057
OMAiAPHKSNT
PhylomeDBiQ9QZ15

Miscellaneous databases

PROiPR:Q9QZ15
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030148 Expressed in 111 organ(s), highest expression level in bone marrow macrophage
CleanExiMM_CLEC4A2
ExpressionAtlasiQ9QZ15 baseline and differential
GenevisibleiQ9QZ15 MM

Family and domain databases

CDDicd03590 CLECT_DC-SIGN_like, 1 hit
Gene3Di3.10.100.10, 1 hit
InterProiView protein in InterPro
IPR001304 C-type_lectin-like
IPR016186 C-type_lectin-like/link_sf
IPR018378 C-type_lectin_CS
IPR033989 CD209-like_CTLD
IPR016187 CTDL_fold
PfamiView protein in Pfam
PF00059 Lectin_C, 1 hit
SMARTiView protein in SMART
SM00034 CLECT, 1 hit
SUPFAMiSSF56436 SSF56436, 1 hit
PROSITEiView protein in PROSITE
PS00615 C_TYPE_LECTIN_1, 1 hit
PS50041 C_TYPE_LECTIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLC4A_MOUSE
AccessioniPrimary (citable) accession number: Q9QZ15
Secondary accession number(s): Q4VA33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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