Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acyl-coenzyme A thioesterase 2, mitochondrial

Gene

Acot2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Most active on substrates with chain lengths ranging from C14-C20. The enzyme is involved in enhancing the hepatic fatty acid oxidation in meitochondria.1 Publication

Catalytic activityi

Palmitoyl-CoA + H2O = CoA + palmitate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei273Charge relay systemBy similarity1
Active sitei365Charge relay systemBy similarity1
Active sitei399Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase

Enzyme and pathway databases

ReactomeiR-MMU-77289 Mitochondrial Fatty Acid Beta-Oxidation
R-MMU-9033241 Peroxisomal protein import

Protein family/group databases

ESTHERimouse-acot2 Acyl-CoA_Thioesterase
MEROPSiS09.A52

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 2, mitochondrial (EC:3.1.2.21 Publication)
Short name:
Acyl-CoA thioesterase 2
Alternative name(s):
Acyl coenzyme A thioester hydrolase
MTE-I
Very-long-chain acyl-CoA thioesterase
Gene namesi
Name:Acot2
Synonyms:Mte1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2159605 Acot2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3259488

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionSequence analysisAdd BLAST42
ChainiPRO_000003406543 – 453Acyl-coenzyme A thioesterase 2, mitochondrialAdd BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei83N6-acetyllysineCombined sources1
Modified residuei447N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9QYR9
MaxQBiQ9QYR9
PaxDbiQ9QYR9
PeptideAtlasiQ9QYR9
PRIDEiQ9QYR9

PTM databases

iPTMnetiQ9QYR9
PhosphoSitePlusiQ9QYR9

Expressioni

Tissue specificityi

Highly expressed in brown and white adipose tissue, muscle, heart, kidney, lung, adrenal gland and spleen; weakly expressed in intestine, testis and brain.2 Publications

Inductioni

In the liver, by peroxisome proliferator (Clofibrate) treatment, via the peroxisome proliferator-activated receptors (PPARs) or fasting for 24 hours.1 Publication

Gene expression databases

BgeeiENSMUSG00000021226 Expressed in 273 organ(s), highest expression level in cardiac ventricle
GenevisibleiQ9QYR9 MM

Interactioni

Subunit structurei

Monomer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9QYR9, 3 interactors
MINTiQ9QYR9
STRINGi10090.ENSMUSP00000021649

Structurei

3D structure databases

ProteinModelPortaliQ9QYR9
SMRiQ9QYR9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the C/M/P thioester hydrolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410II3X Eukaryota
COG1073 LUCA
GeneTreeiENSGT00390000001046
HOGENOMiHOG000116219
HOVERGENiHBG000331
InParanoidiQ9QYR9
KOiK01068
OMAiGTYASFC
OrthoDBiEOG091G08KU
TreeFamiTF314911

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR016662 Acyl-CoA_thioEstase_long-chain
IPR014940 BAAT_C
IPR006862 Thio_Ohase/aa_AcTrfase
PfamiView protein in Pfam
PF08840 BAAT_C, 1 hit
PF04775 Bile_Hydr_Trans, 1 hit
PIRSFiPIRSF016521 Acyl-CoA_hydro, 1 hit
SUPFAMiSSF53474 SSF53474, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QYR9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVASSFAVLR ASRLCQQDWK SWARLFVPPP LSTGGRTTWA RTNATLSVEP
60 70 80 90 100
EGRSCWDEPL SIAVRGLAPE QPVTLRSALR DEKGALFRAH ARYRADAGGE
110 120 130 140 150
LNLARAPALG GSFSGLEPMG LLWAMEPERP LWRLIKRDVQ TPFLVELEVL
160 170 180 190 200
DGHEPDGGQR LAQAVHERHF LAPGVRRVPV REGRVRATLF LPPEPGPFPG
210 220 230 240 250
IIDLFGVGGG LLEYRASLLA GKGFAVMALA YYNYDDLPKS IETMHMEYFE
260 270 280 290 300
EAVNYLRSHP EVKGPGIGLL GISKGGELGL AMASFLKGIT AAVVINGSVA
310 320 330 340 350
AVGNTISYKD ETIPPVSLLR NQVKMTKDGL LDVVEALQSP LVDKKSFIPV
360 370 380 390 400
ERSDTTFLFL VGQDDHNWKS EFYADEISKR LQAHGKEKPQ IICYPAAGHY
410 420 430 440 450
IEPPYFPLCS AGMHLLVGAN ITFGGEPRAH AVAQVDAWQQ LQTFFHKQLG

SKS
Length:453
Mass (Da):49,657
Last modified:July 27, 2011 - v2
Checksum:i5687F2363B16284B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163Q → H in AAF13871 (PubMed:10567408).Curated1
Sequence conflicti319L → V in AAF13871 (PubMed:10567408).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180798, AF180796, AF180797 Genomic DNA Translation: AAF13871.1
AK172617 mRNA Translation: BAE43095.1
CH466590 Genomic DNA Translation: EDL02755.1
CCDSiCCDS26034.1
RefSeqiNP_598949.3, NM_134188.3
UniGeneiMm.371675

Genome annotation databases

EnsembliENSMUST00000021649; ENSMUSP00000021649; ENSMUSG00000021226
GeneIDi171210
KEGGimmu:171210
UCSCiuc011yor.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180798, AF180796, AF180797 Genomic DNA Translation: AAF13871.1
AK172617 mRNA Translation: BAE43095.1
CH466590 Genomic DNA Translation: EDL02755.1
CCDSiCCDS26034.1
RefSeqiNP_598949.3, NM_134188.3
UniGeneiMm.371675

3D structure databases

ProteinModelPortaliQ9QYR9
SMRiQ9QYR9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QYR9, 3 interactors
MINTiQ9QYR9
STRINGi10090.ENSMUSP00000021649

Chemistry databases

ChEMBLiCHEMBL3259488

Protein family/group databases

ESTHERimouse-acot2 Acyl-CoA_Thioesterase
MEROPSiS09.A52

PTM databases

iPTMnetiQ9QYR9
PhosphoSitePlusiQ9QYR9

Proteomic databases

EPDiQ9QYR9
MaxQBiQ9QYR9
PaxDbiQ9QYR9
PeptideAtlasiQ9QYR9
PRIDEiQ9QYR9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021649; ENSMUSP00000021649; ENSMUSG00000021226
GeneIDi171210
KEGGimmu:171210
UCSCiuc011yor.1 mouse

Organism-specific databases

CTDi10965
MGIiMGI:2159605 Acot2

Phylogenomic databases

eggNOGiENOG410II3X Eukaryota
COG1073 LUCA
GeneTreeiENSGT00390000001046
HOGENOMiHOG000116219
HOVERGENiHBG000331
InParanoidiQ9QYR9
KOiK01068
OMAiGTYASFC
OrthoDBiEOG091G08KU
TreeFamiTF314911

Enzyme and pathway databases

ReactomeiR-MMU-77289 Mitochondrial Fatty Acid Beta-Oxidation
R-MMU-9033241 Peroxisomal protein import

Miscellaneous databases

PROiPR:Q9QYR9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021226 Expressed in 273 organ(s), highest expression level in cardiac ventricle
GenevisibleiQ9QYR9 MM

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR016662 Acyl-CoA_thioEstase_long-chain
IPR014940 BAAT_C
IPR006862 Thio_Ohase/aa_AcTrfase
PfamiView protein in Pfam
PF08840 BAAT_C, 1 hit
PF04775 Bile_Hydr_Trans, 1 hit
PIRSFiPIRSF016521 Acyl-CoA_hydro, 1 hit
SUPFAMiSSF53474 SSF53474, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiACOT2_MOUSE
AccessioniPrimary (citable) accession number: Q9QYR9
Secondary accession number(s): Q3T9C9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again