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Protein

5-azacytidine-induced protein 2

Gene

Azi2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.1 Publication

GO - Biological processi

  • cytokine production Source: MGI
  • dendritic cell differentiation Source: MGI
  • dendritic cell proliferation Source: MGI
  • I-kappaB kinase/NF-kappaB signaling Source: MGI
  • interferon-alpha production Source: MGI
  • interferon-gamma production Source: MGI
  • interleukin-6 production Source: MGI
  • mitotic cell cycle Source: MGI
  • T cell activation Source: MGI
  • tumor necrosis factor production Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
5-azacytidine-induced protein 2
Alternative name(s):
NF-kappa-B-activating kinase-associated protein 1
Short name:
Nak-associated protein 1
Gene namesi
Name:Azi2
Synonyms:Az2, Nap1, Tbkp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1351332 Azi2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi236Y → A: Abolishes interaction with TBK1 but not with IKBKE. 1 Publication1
Mutagenesisi237W → A: No effect on interaction with TBK1 and IKBKE. 1 Publication1
Mutagenesisi238E → A: No effect on interaction with TBK1 and IKBKE. 1 Publication1
Mutagenesisi253Q → A: Abolishes interaction with TBK1 and IKBKE. 1 Publication1
Mutagenesisi257L → A: Abolishes interaction with TBK1 and IKBKE. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002806041 – 4055-azacytidine-induced protein 2Add BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei331PhosphoserineBy similarity1
Modified residuei366PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QYP6
PeptideAtlasiQ9QYP6
PRIDEiQ9QYP6

PTM databases

iPTMnetiQ9QYP6
PhosphoSitePlusiQ9QYP6

Expressioni

Tissue specificityi

Testis, ovary, heart, lung, kidney and brain. Expressed mainly in the spermatocytes or spermatids in the testis.1 Publication

Inductioni

By 5-azacytidine.1 Publication

Gene expression databases

BgeeiENSMUSG00000039285 Expressed in 290 organ(s), highest expression level in testis
CleanExiMM_AZI2
ExpressionAtlasiQ9QYP6 baseline and differential
GenevisibleiQ9QYP6 MM

Interactioni

Subunit structurei

Homodimer. Interacts with IKBKE, TBK1 and TICAM1.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi205136, 1 interactor
IntActiQ9QYP6, 11 interactors
STRINGi10090.ENSMUSP00000044350

Structurei

3D structure databases

ProteinModelPortaliQ9QYP6
SMRiQ9QYP6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 198HomodimerizationAdd BLAST198
Regioni229 – 270Interaction with TBK1 and IKBKE1 PublicationAdd BLAST42

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili40 – 197Sequence analysisAdd BLAST158

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG4Z Eukaryota
ENOG4111IT0 LUCA
GeneTreeiENSGT00390000001279
HOGENOMiHOG000013059
HOVERGENiHBG067551
InParanoidiQ9QYP6
KOiK12651
OMAiANSELQI
OrthoDBiEOG091G09UX
PhylomeDBiQ9QYP6
TreeFamiTF331289

Family and domain databases

InterProiView protein in InterPro
IPR024581 TBD
PfamiView protein in Pfam
PF12845 TBD, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9QYP6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDTLVEDDIC ILNHEKAHRR EAVTPLSAYP GDESVASHFA LVTAYEDIKK
60 70 80 90 100
RLKDSEKENS FLKKRIRALE ERLVGARADE ETSSVGREQV NKAYHAYREV
110 120 130 140 150
CIDRDNLKNQ LEKINKDNSE SLKMLNEQLQ SKEVELLQLR TEVETQQVMR
160 170 180 190 200
NLNPPSSSWE VEKLSCDLKI HGLEQELGLL RKECSDLRTE LQKARQTGPP
210 220 230 240 250
QEDILQGRDV IRPSLSREEH VPHQGLHHSD NMQHAYWELK REMSNLHLVT
260 270 280 290 300
QVQAELLRKL KTSAAVKKAC TPVGCVEDLG RDSTKLHLTN FTATYKRHPS
310 320 330 340 350
LSPNGKAPCY APSSPLPGDR KVFSDKAVLQ SWTDNERLVP NDGADFPEHS
360 370 380 390 400
SYGRNSLEDN SWVFPSPPKS SETAFGENKS KILPLSNLPP LHYLDQQNQN

CLYKS
Length:405
Mass (Da):46,091
Last modified:May 1, 2000 - v1
Checksum:i5EBD91369CBCB89F
GO
Isoform 2 (identifier: Q9QYP6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     269-283: ACTPVGCVEDLGRDS → GKSLLAANADSSQTA
     284-405: Missing.

Show »
Length:283
Mass (Da):32,389
Checksum:iB51C5A60133C9236
GO
Isoform 3 (identifier: Q9QYP6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-405: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:114
Mass (Da):13,130
Checksum:iB80F9AF6016B8487
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z7S3D3Z7S3_MOUSE
5-azacytidine-induced protein 2
Azi2
178Annotation score:
F7A092F7A092_MOUSE
5-azacytidine-induced protein 2
Azi2
199Annotation score:
F6SY96F6SY96_MOUSE
5-azacytidine-induced protein 2
Azi2
67Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023822115 – 405Missing in isoform 3. 1 PublicationAdd BLAST291
Alternative sequenceiVSP_023823269 – 283ACTPV…LGRDS → GKSLLAANADSSQTA in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_023824284 – 405Missing in isoform 2. 1 PublicationAdd BLAST122

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007141 mRNA Translation: BAA88213.1
AK004992 mRNA Translation: BAB23727.1
AK015942 mRNA Translation: BAB30044.1
AK030076 mRNA Translation: BAC26770.1
AK036102 mRNA Translation: BAE43294.1
AK153477 mRNA Translation: BAE32027.1
AK165549 mRNA Translation: BAE38249.1
AK167783 mRNA Translation: BAE39815.1
BC006851 mRNA Translation: AAH06851.1
CCDSiCCDS40799.1 [Q9QYP6-1]
CCDS52959.1 [Q9QYP6-2]
RefSeqiNP_001041611.1, NM_001048146.2 [Q9QYP6-2]
NP_001273436.1, NM_001286507.1
NP_001273437.1, NM_001286508.1 [Q9QYP6-1]
NP_038755.1, NM_013727.4 [Q9QYP6-1]
UniGeneiMm.92705

Genome annotation databases

EnsembliENSMUST00000044454; ENSMUSP00000044350; ENSMUSG00000039285 [Q9QYP6-1]
ENSMUST00000133580; ENSMUSP00000118765; ENSMUSG00000039285 [Q9QYP6-1]
ENSMUST00000134433; ENSMUSP00000114980; ENSMUSG00000039285 [Q9QYP6-2]
ENSMUST00000154583; ENSMUSP00000122063; ENSMUSG00000039285 [Q9QYP6-3]
GeneIDi27215
KEGGimmu:27215
UCSCiuc009rzi.2 mouse [Q9QYP6-2]
uc009rzj.2 mouse [Q9QYP6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007141 mRNA Translation: BAA88213.1
AK004992 mRNA Translation: BAB23727.1
AK015942 mRNA Translation: BAB30044.1
AK030076 mRNA Translation: BAC26770.1
AK036102 mRNA Translation: BAE43294.1
AK153477 mRNA Translation: BAE32027.1
AK165549 mRNA Translation: BAE38249.1
AK167783 mRNA Translation: BAE39815.1
BC006851 mRNA Translation: AAH06851.1
CCDSiCCDS40799.1 [Q9QYP6-1]
CCDS52959.1 [Q9QYP6-2]
RefSeqiNP_001041611.1, NM_001048146.2 [Q9QYP6-2]
NP_001273436.1, NM_001286507.1
NP_001273437.1, NM_001286508.1 [Q9QYP6-1]
NP_038755.1, NM_013727.4 [Q9QYP6-1]
UniGeneiMm.92705

3D structure databases

ProteinModelPortaliQ9QYP6
SMRiQ9QYP6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205136, 1 interactor
IntActiQ9QYP6, 11 interactors
STRINGi10090.ENSMUSP00000044350

PTM databases

iPTMnetiQ9QYP6
PhosphoSitePlusiQ9QYP6

Proteomic databases

PaxDbiQ9QYP6
PeptideAtlasiQ9QYP6
PRIDEiQ9QYP6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044454; ENSMUSP00000044350; ENSMUSG00000039285 [Q9QYP6-1]
ENSMUST00000133580; ENSMUSP00000118765; ENSMUSG00000039285 [Q9QYP6-1]
ENSMUST00000134433; ENSMUSP00000114980; ENSMUSG00000039285 [Q9QYP6-2]
ENSMUST00000154583; ENSMUSP00000122063; ENSMUSG00000039285 [Q9QYP6-3]
GeneIDi27215
KEGGimmu:27215
UCSCiuc009rzi.2 mouse [Q9QYP6-2]
uc009rzj.2 mouse [Q9QYP6-1]

Organism-specific databases

CTDi64343
MGIiMGI:1351332 Azi2

Phylogenomic databases

eggNOGiENOG410IG4Z Eukaryota
ENOG4111IT0 LUCA
GeneTreeiENSGT00390000001279
HOGENOMiHOG000013059
HOVERGENiHBG067551
InParanoidiQ9QYP6
KOiK12651
OMAiANSELQI
OrthoDBiEOG091G09UX
PhylomeDBiQ9QYP6
TreeFamiTF331289

Miscellaneous databases

ChiTaRSiAzi2 mouse
PROiPR:Q9QYP6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039285 Expressed in 290 organ(s), highest expression level in testis
CleanExiMM_AZI2
ExpressionAtlasiQ9QYP6 baseline and differential
GenevisibleiQ9QYP6 MM

Family and domain databases

InterProiView protein in InterPro
IPR024581 TBD
PfamiView protein in Pfam
PF12845 TBD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAZI2_MOUSE
AccessioniPrimary (citable) accession number: Q9QYP6
Secondary accession number(s): Q3TIN1, Q3V3R2, Q9CUI5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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