UniProtKB - Q9QYM0 (MRP5_RAT)
ATP-binding cassette sub-family C member 5
Abcc5
Functioni
ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Mediates ATP-dependent transport of endogenous metabolites such as cAMP and cGMP, folic acid and N-lactoyl-amino acids (in vitro). Acts also as a general glutamate conjugate and analog transporter that can limit the brain levels of endogenous metabolites, drugs, and toxins. Confers resistance to the antiviral agent PMEA. Able to transport several anticancer drugs including methotrexate, and nucleotide analogs in vitro, however it does with low affinity, thus the exact role of ABCC5 in mediating resistance still needs to be elucidated. Acts as a heme transporter required for the translocation of cytosolic heme to the secretory pathway (By similarity).
May play a role in energy metabolism by regulating the glucagon-like peptide 1 (GLP-1) secretion from enteroendocrine cells (By similarity).
By similarityMiscellaneous
Catalytic activityi
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- ATP + H2O + N-acetyl-L-aspartyl-L-glutamate(in) = ADP + H+ + N-acetyl-L-aspartyl-L-glutamate(out) + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H2O + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate(in) = ADP + H+ + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate(out) + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H2O + N-acetyl-L-glutamate(in) = ADP + H+ + N-acetyl-L-glutamate(out) + phosphateBy similarity
- ATP + H2O + N-acetyl-L-aspartate(in) = ADP + H+ + N-acetyl-L-aspartate(out) + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- (2S)-2-[5-amino-1-(β-D-ribosyl)imidazole-4-carboxamido]succinate(in) + ATP + H2O = (2S)-2-[5-amino-1-(β-D-ribosyl)imidazole-4-carboxamido]succinate(out) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- ATP + H2O + N-[(S)-lactoyl]-L-phenylalanine(in) = ADP + H+ + N-[(S)-lactoyl]-L-phenylalanine(out) + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2).By similarity EC:7.6.2.2
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 595 – 602 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 1226 – 1233 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type xenobiotic transporter activity Source: UniProtKB
- ATPase-coupled transmembrane transporter activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- efflux transmembrane transporter activity Source: RGD
- glutathione transmembrane transporter activity Source: RGD
- heme transmembrane transporter activity Source: UniProtKB
- purine nucleotide transmembrane transporter activity Source: RGD
- xenobiotic transmembrane transporter activity Source: RGD
GO - Biological processi
- cAMP transport Source: RGD
- cGMP transport Source: RGD
- export across plasma membrane Source: RGD
- folate transmembrane transport Source: UniProtKB
- glutathione transmembrane transport Source: RGD
- heme transmembrane transport Source: UniProtKB
- purine nucleotide transport Source: RGD
- response to insulin Source: RGD
- response to lipopolysaccharide Source: RGD
- transmembrane transport Source: GO_Central
- xenobiotic transport Source: RGD
Keywordsi
Molecular function | Translocase |
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-2142850, Hyaluronan biosynthesis and export R-RNO-382556, ABC-family proteins mediated transport |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 70913, Abcc5 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus lumen By similarity
Endosome
- Endosome membrane By similarity
Plasma membrane
- Basolateral cell membrane By similarity; Multi-pass membrane protein Sequence analysis
- Apical cell membrane By similarity; Multi-pass membrane protein Sequence analysis
Other locations
- Cytoplasmic granule By similarity
Note: In most cells, routes to the basolateral plasma membrane, but in the brain capillary endothelial cells that form the blood-brain barrier, resides in the apical membrane.By similarity
Endosome
- endosome membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi lumen Source: UniProtKB-SubCell
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- basolateral plasma membrane Source: ARUK-UCL
- plasma membrane Source: RGD
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: RGD
- perinuclear region of cytoplasm Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 179 – 199 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 219 – 239 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 296 – 316 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 317 – 337 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 400 – 420 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 426 – 446 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 608 – 628 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 847 – 867 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 916 – 936 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 996 – 1016 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1017 – 1037 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1101 – 1121 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1126 – 1146 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, Endosome, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093365 | 1 – 1436 | ATP-binding cassette sub-family C member 5Add BLAST | 1436 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | PhosphoserineBy similarity | 1 | |
Modified residuei | 19 | PhosphoserineBy similarity | 1 | |
Modified residuei | 43 | PhosphoserineBy similarity | 1 | |
Modified residuei | 60 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 494 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 505 | PhosphoserineBy similarity | 1 | |
Modified residuei | 509 | PhosphoserineBy similarity | 1 | |
Modified residuei | 513 | PhosphothreonineBy similarity | 1 | |
Glycosylationi | 636 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 684 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 889 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 896 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1043 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1328 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1416 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | Q9QYM0 |
PRIDEi | Q9QYM0 |
PTM databases
GlyGeni | Q9QYM0, 8 sites |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000029178, Expressed in stomach and 20 other tissues |
ExpressionAtlasi | Q9QYM0, baseline and differential |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 179 – 459 | ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST | 281 | |
Domaini | 562 – 783 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 222 | |
Domaini | 858 – 1154 | ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST | 297 | |
Domaini | 1192 – 1426 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 235 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 12 – 40 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 499 – 569 | DisorderedSequence analysisAdd BLAST | 71 | |
Regioni | 795 – 822 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 25 – 39 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 548 – 569 | Basic and acidic residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 796 – 810 | Polar residuesSequence analysisAdd BLAST | 15 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0054, Eukaryota |
InParanoidi | Q9QYM0 |
OMAi | FVKFFGW |
OrthoDBi | 138195at2759 |
PhylomeDBi | Q9QYM0 |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR030238, ABCC5 IPR027417, P-loop_NTPase |
PANTHERi | PTHR24223:SF196, PTHR24223:SF196, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MKDIDMGKEY IIPSPGYRSV RDRSTIPGTH GDREDPSSGE QISLECQDAL
60 70 80 90 100
ETAARVEGLS LDISVHSHLQ ILDEEHTKGK YHHGLSALKP FRTTTKHQHP
110 120 130 140 150
VDNAGLFSYM TFSWLSPLAQ VVHKKGELLM EDVWPLSKYE SSDVNCRRLE
160 170 180 190 200
RLWQEELNEV GPDAASLRRV VWIFCRTRLI LSIVCLMITQ LAGFSGPAFV
210 220 230 240 250
VKHLLEYTQA TESNLQYSLL LVLGLLLTEV VRSWSLALTW ALNYRTGVRL
260 270 280 290 300
RGAVLTMAFK KILKLKNIKE KSLGELINIC SNDGQRMFEA AAVGSLLAGG
310 320 330 340 350
PVVAILGMIY NVIILGPTGF LGSAVFILFY PAMMFVSRLT AYFRRKCVAA
360 370 380 390 400
TDDRVQKMNE VLTYIKFIKM YAWVKAFSQC VQKIREEERR ILEKAGYFQS
410 420 430 440 450
ITVGVAPIVV VIASVVTFSV HMTLGFDLTA AQAFTVVTVF NSMTFALKVT
460 470 480 490 500
PFSVKSLSEA SVAVDRFKSL FLMEEVHMIK NKPASPHIKI EMKNATLAWD
510 520 530 540 550
SSHSSTQSSP KLTPKVKKDK RAPKGKKEKS RQLQHTEHQA VLAEQKGHLL
560 570 580 590 600
LDSDERPSPE EEEGKQIHAG SMRLQRTLYN IDLEIEEGKL VGICGSVGSG
610 620 630 640 650
KTSLISAILG QMTLLEGSIA VSGTFAYVAQ QAWILNATLR DNILFGKEFD
660 670 680 690 700
EERYNSVLNS CCLRPDLAIL PNSDLTEIGE RGANLSGGQR QRISLARALY
710 720 730 740 750
SDRSIYILDD PLSALDAHVG NHIFNSAIRK RLKSKTVLFV THQLQYLVDC
760 770 780 790 800
DEVIFMKEGC ITERGTHEEL MNLNGDYATI FNNLLLGETP PVEINSKKEA
810 820 830 840 850
SGSQKSQDKG PKPGSVKKEK AVKSEEGQLV QVEEKGQGSV PWSVYWVYIQ
860 870 880 890 900
AAGGPLAFLV IMVLFMLNVG STAFSTWWLS YWIKQGSGNS TVFEGNRSSV
910 920 930 940 950
SDSMRDNPFL QYYASIYALS MAVMLILKAI RGVVFVKGTL RASSRLHDEL
960 970 980 990 1000
FRRILRSPMK FFDTTPTGRI LNRFSKDMDE VDVRLPFQAE MFIQNVILVF
1010 1020 1030 1040 1050
FCVGMIAGVF PWFLVAVGPL LILFSVLHIV SRVLIRELKR LDNITQSPFL
1060 1070 1080 1090 1100
SHITSSIQGL ATIHAYNKRQ EFLHRYQELL DDNQAPFFLF TCAMRWLAVR
1110 1120 1130 1140 1150
LDLISIALIT TTGLMIVLMH GQIPSAYAGL AISYAVQLTG LFQFTVRLAS
1160 1170 1180 1190 1200
ETEARFTSVE RINHYIKTLS LEAPARIKNK APPHDWPQEG EITFENAEMR
1210 1220 1230 1240 1250
YRENLPLVLK KVSFTIKPKE KIGIVGRTGS GKSSLGMALF RLVELSGGCI
1260 1270 1280 1290 1300
KIDGVRISDI GLADLRSKLA IIPQEPVLFS GTVRSNLDPF NQYTEEQIWD
1310 1320 1330 1340 1350
ALERTHMKEC IAQLPLKLES EVMENGDNFS VGERQLLCIA RALLRHCKIL
1360 1370 1380 1390 1400
ILDEATAAMD TETDLLIQET IREAFADCTM LTIAHRLHTV LGSDRIMVLA
1410 1420 1430
QGQVVEFDTP SVLLSNDSSR FYAMCAAAEN KVAVKG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V676 | G3V676_RAT | Multidrug resistance-associated pro... | Abcc5 | 1,434 | Annotation score: | ||
F7EV50 | F7EV50_RAT | Multidrug resistance-associated pro... | Abcc5 | 222 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 29 | T → Q in BAA88897 (Ref. 1) | 1 | |
Sequence conflicti | 35 – 42 | DPSSGEQI → EPRFRRTR in BAA88897 (Ref. 1) | 8 | |
Sequence conflicti | 1270 | A → T in BAA88897 (Ref. 1) | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB020209 mRNA Translation: BAA88897.1 AABR07034695 Genomic DNA No translation available. AABR07034696 Genomic DNA No translation available. |
RefSeqi | NP_446376.1, NM_053924.1 |
Genome annotation databases
GeneIDi | 116721 |
KEGGi | rno:116721 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB020209 mRNA Translation: BAA88897.1 AABR07034695 Genomic DNA No translation available. AABR07034696 Genomic DNA No translation available. |
RefSeqi | NP_446376.1, NM_053924.1 |
3D structure databases
SMRi | Q9QYM0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000002316 |
PTM databases
GlyGeni | Q9QYM0, 8 sites |
Proteomic databases
PaxDbi | Q9QYM0 |
PRIDEi | Q9QYM0 |
Genome annotation databases
GeneIDi | 116721 |
KEGGi | rno:116721 |
Organism-specific databases
CTDi | 10057 |
RGDi | 70913, Abcc5 |
Phylogenomic databases
eggNOGi | KOG0054, Eukaryota |
InParanoidi | Q9QYM0 |
OMAi | FVKFFGW |
OrthoDBi | 138195at2759 |
PhylomeDBi | Q9QYM0 |
Enzyme and pathway databases
Reactomei | R-RNO-2142850, Hyaluronan biosynthesis and export R-RNO-382556, ABC-family proteins mediated transport |
Miscellaneous databases
PROi | PR:Q9QYM0 |
Gene expression databases
Bgeei | ENSRNOG00000029178, Expressed in stomach and 20 other tissues |
ExpressionAtlasi | Q9QYM0, baseline and differential |
Family and domain databases
Gene3Di | 1.20.1560.10, 2 hits 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR030238, ABCC5 IPR027417, P-loop_NTPase |
PANTHERi | PTHR24223:SF196, PTHR24223:SF196, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 2 hits PS50893, ABC_TRANSPORTER_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | MRP5_RAT | |
Accessioni | Q9QYM0Primary (citable) accession number: Q9QYM0 Secondary accession number(s): A0A0G2K6R4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | September 29, 2021 | |
Last modified: | February 23, 2022 | |
This is version 132 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families