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Protein

Zinc finger protein PLAG1

Gene

Plag1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Cooperates with CBFB-MYH11.3 Publications

Miscellaneous

Mice overexpressing Plag1 develop normally with high Plag1 transgene expression in salivary glands, spleen and weak mammary gland detection. They develop salivary gland tumors with major pathological features of human pleimorphic adenomas at the age of 1-5 months.
Mice with Plag1 overexpression targeted to salivary and mammary gland develop multifocal salivary gland tumors with pathological features of human pleimorphic adenomas at the age of 5 weeks, as well as mammary gland tumors at the age of 1 year. Plag1 overexpression in salivary gland are accompanied by increased IGFII expression. Besides features characteristic of benign pleimorphic adenomas, malignant characteristics are also observed in salivary gland tumors as well as metastasis to the lungs, reinforcing the tumorigenic role of Plag1.
Neonate mice carrying a human myeloid leukemia translocation-associated fusion gene CBFB-MYH11, injected with a retrovirus (4070A), develop the pathology within 2-5 months due to few viral insertions in some genes, such as PLAG1. Insertions near the transcription start site of PLAG1 are predominant in the related tumors and probably participate in the transformation process. Plag1 is one candidate gene that can cooperate with CBFB-MYH11 for leukemogenesis in this mouse model.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri34 – 56C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri62 – 86C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri92 – 114C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri121 – 143C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri150 – 172C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri185 – 207C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri213 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein PLAG1
Alternative name(s):
Pleiomorphic adenoma gene 1 protein
Gene namesi
Name:Plag1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1891916 Plag1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice exhibit growth retardation with lower birth weight and disproportionally small seminal vesicles and ventral prostate as well as decreased fertility in both male and female. However, IGFII expression is normal in embryos.1 Publication

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002951081 – 499Zinc finger protein PLAG1Add BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki244Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki263Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Sumoylated with SUMO1; which inhibits transcriptional activity, but does not affect nuclear localization. Blockers of sumoylation pathway such as SENP3 and inactive UBE2I increases transcriptional capacity. Sumoylation is increased in the presence of PIAS1 (By similarity).By similarity
Acetylated by lysine acetyltransferase EP300; which activates transcriptional capacity. Lysine residues that are sumoylated also seem to be target for acetylation (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ9QYE0
PaxDbiQ9QYE0
PRIDEiQ9QYE0

PTM databases

PhosphoSitePlusiQ9QYE0

Expressioni

Tissue specificityi

Expressed in heart, spleen, lung, kidney, brain, testis and epididymis but not in salivary glands.1 Publication

Developmental stagei

High fetal expression with a strong decline after birth. Expressed at E9.5 and E10.5 in nervous system with highest level in telencephalon, diencephalon, and midbrain, as well as regionalized expression in the neural tube, which is characteristic of genes involved in the specification of neuronal fates.2 Publications

Gene expression databases

BgeeiENSMUSG00000003282 Expressed in 92 organ(s), highest expression level in secondary oocyte
CleanExiMM_PLAG1
ExpressionAtlasiQ9QYE0 baseline and differential
GenevisibleiQ9QYE0 MM

Interactioni

Subunit structurei

Interacts with KPNA2, which escorts protein to the nucleus via interaction with nuclear localization signal. Interacts with E3 SUMO-protein ligase PIAS1, PIAS2 and PIAS4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003369

Structurei

3D structure databases

ProteinModelPortaliQ9QYE0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 84Interaction with KPNA2By similarityAdd BLAST83
Regioni41 – 242Decreased nuclear import with localization in the nucleus but also in the cytoplasmBy similarityAdd BLAST202
Regioni243 – 499Activates transcription; Inhibition of nuclear import due to lack of NLS and KPNA2 interactionBy similarityAdd BLAST257
Regioni243 – 383Repression domain; contains 3 sumoylation motifs and massively decrease transcription activityBy similarityAdd BLAST141
Regioni384 – 499Massively activates transcriptionBy similarityAdd BLAST116

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi22 – 25Nuclear localization signalBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi369 – 488Ser-richAdd BLAST120

Domaini

C2H2-type zinc fingers 3 interacts with DNA-binding site G-clusterinc fingers. C2H2-type zinc fingers 6 and 7 interact with DNA-binding site core sequence (By similarity).By similarity

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri34 – 56C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri62 – 86C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri92 – 114C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri121 – 143C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri150 – 172C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri185 – 207C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri213 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00390000013640
HOGENOMiHOG000294191
HOVERGENiHBG053608
InParanoidiQ9QYE0
KOiK19484
OMAiIDMDAVH
OrthoDBiEOG091G061O
TreeFamiTF332024

Family and domain databases

InterProiView protein in InterPro
IPR027765 PLAG1
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR44197:SF2 PTHR44197:SF2, 1 hit
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 7 hits
SUPFAMiSSF57667 SSF57667, 4 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 7 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9QYE0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATVIPGDLS EVRDTQKAPS GKRKRGESKP RKNFPCQLCD KAFNSVEKLK
60 70 80 90 100
VHSFSHTGER PYKCTHQDCT KAFVSKYKLQ RHMATHSPEK THKCNYCEKM
110 120 130 140 150
FHRKDHLKNH LHTHDPNKET FKCEECGKSY NTKLGFKRHL ALHAATSGDL
160 170 180 190 200
TCKVCLQNFE STGVLLEHLK SHAGKSSGGV KEKKHQCEHC ERRFYTRKDV
210 220 230 240 250
RRHMVVHTGR KDFLCQYCAQ RFGRKDHLTR HMKKSHNQEL LKVKTEPVDF
260 270 280 290 300
LDPFTCNMSV PIKDELLPVM SLPSSELLSK PFTNTLQLNL YNTPFQSMQS
310 320 330 340 350
SGSAHQMITT LPLGMTCPID MDAVHPSHHL AFKCPFSSTS YAISIPEKEQ
360 370 380 390 400
PLKGEIESYL MELQGGAPSS SQDSPASSSK LGLEPQSGSP DDGAGDLSLS
410 420 430 440 450
KSSISISDPL STPALDFSQL FNFIPLNGPP YNPLSVGSLG MSYSQEEAHS
460 470 480 490
SVSQLPTQTQ DLQDPANTVG LSSLHSLSAA FTSSLSSSTT LPRFHQAFQ
Length:499
Mass (Da):55,579
Last modified:July 27, 2011 - v2
Checksum:iA3493D2DADCB83B2
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RES7D6RES7_MOUSE
Zinc finger protein PLAG1
Plag1
43Annotation score:

Sequence cautioni

The sequence BAE22643 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti433P → T in AAS86161 (PubMed:15585652).Curated1
Sequence conflicti433P → T in AAF21762 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY574219 mRNA Translation: AAS86161.1
AF057366 mRNA Translation: AAF21762.1
BC139283 mRNA Translation: AAI39284.1
BC139285 mRNA Translation: AAI39286.1
AL807387 Genomic DNA Translation: CAM18646.1
CH466538 Genomic DNA Translation: EDL05712.1
AK135759 mRNA Translation: BAE22643.1 Different initiation.
CCDSiCCDS17941.1
RefSeqiNP_064353.2, NM_019969.3
XP_006538179.1, XM_006538116.3
XP_011248381.1, XM_011250079.2
XP_017175819.1, XM_017320330.1
XP_017175820.1, XM_017320331.1
UniGeneiMm.331467

Genome annotation databases

EnsembliENSMUST00000003369; ENSMUSP00000003369; ENSMUSG00000003282
GeneIDi56711
KEGGimmu:56711
UCSCiuc008rwp.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY574219 mRNA Translation: AAS86161.1
AF057366 mRNA Translation: AAF21762.1
BC139283 mRNA Translation: AAI39284.1
BC139285 mRNA Translation: AAI39286.1
AL807387 Genomic DNA Translation: CAM18646.1
CH466538 Genomic DNA Translation: EDL05712.1
AK135759 mRNA Translation: BAE22643.1 Different initiation.
CCDSiCCDS17941.1
RefSeqiNP_064353.2, NM_019969.3
XP_006538179.1, XM_006538116.3
XP_011248381.1, XM_011250079.2
XP_017175819.1, XM_017320330.1
XP_017175820.1, XM_017320331.1
UniGeneiMm.331467

3D structure databases

ProteinModelPortaliQ9QYE0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003369

PTM databases

PhosphoSitePlusiQ9QYE0

Proteomic databases

MaxQBiQ9QYE0
PaxDbiQ9QYE0
PRIDEiQ9QYE0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003369; ENSMUSP00000003369; ENSMUSG00000003282
GeneIDi56711
KEGGimmu:56711
UCSCiuc008rwp.2 mouse

Organism-specific databases

CTDi5324
MGIiMGI:1891916 Plag1

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00390000013640
HOGENOMiHOG000294191
HOVERGENiHBG053608
InParanoidiQ9QYE0
KOiK19484
OMAiIDMDAVH
OrthoDBiEOG091G061O
TreeFamiTF332024

Miscellaneous databases

PROiPR:Q9QYE0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003282 Expressed in 92 organ(s), highest expression level in secondary oocyte
CleanExiMM_PLAG1
ExpressionAtlasiQ9QYE0 baseline and differential
GenevisibleiQ9QYE0 MM

Family and domain databases

InterProiView protein in InterPro
IPR027765 PLAG1
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR44197:SF2 PTHR44197:SF2, 1 hit
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 7 hits
SUPFAMiSSF57667 SSF57667, 4 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 7 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPLAG1_MOUSE
AccessioniPrimary (citable) accession number: Q9QYE0
Secondary accession number(s): B1AXC4, Q3UXC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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