UniProtKB - Q9QYC3 (BHA15_MOUSE)
Class A basic helix-loop-helix protein 15
Bhlha15
Functioni
Plays a role in controlling the transcriptional activity of MyoD, ensuring that expanding myoblast populations remain undifferentiated (PubMed:17612490).
Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'.
4 PublicationsGO - Molecular functioni
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- identical protein binding Source: MGI
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: NTNU_SB
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- calcium-mediated signaling Source: MGI
- cell-cell signaling Source: MGI
- cell maturation Source: MGI
- cellular response to glucose starvation Source: UniProtKB
- endoplasmic reticulum unfolded protein response Source: UniProtKB
- glucose homeostasis Source: MGI
- Golgi organization Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- intracellular distribution of mitochondria Source: MGI
- mitochondrial calcium ion transmembrane transport Source: MGI
- negative regulation of myotube differentiation Source: UniProtKB
- neuron differentiation Source: GO_Central
- positive regulation of transcription by RNA polymerase II Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Class A basic helix-loop-helix protein 15Short name: bHLHa15 Alternative name(s): Class B basic helix-loop-helix protein 8 Short name: bHLHb8 Muscle, intestine and stomach expression 1 Short name: MIST-1 |
Gene namesi | Name:Bhlha15 Synonyms:Bhlhb8, Mist1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:891976, Bhlha15 |
VEuPathDBi | HostDB:ENSMUSG00000052271 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127151 | 1 – 197 | Class A basic helix-loop-helix protein 15Add BLAST | 197 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 25 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9QYC3 |
PRIDEi | Q9QYC3 |
ProteomicsDBi | 265211 |
PTM databases
iPTMneti | Q9QYC3 |
PhosphoSitePlusi | Q9QYC3 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000052271, Expressed in dorsal pancreas and 144 other tissues |
Genevisiblei | Q9QYC3, MM |
Interactioni
Subunit structurei
Forms homodimers or heterodimers with TCF3 gene products E12 and E47. These dimers bind to the E-box site, however, heterodimer with MYOD1 does not bind target DNA.
GO - Molecular functioni
- identical protein binding Source: MGI
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 201426, 1 interactor |
STRINGi | 10090.ENSMUSP00000055493 |
Miscellaneous databases
RNActi | Q9QYC3, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 72 – 124 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 82 | DisorderedSequence analysisAdd BLAST | 82 | |
Regioni | 178 – 197 | DisorderedSequence analysisAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 17 – 40 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 52 – 82 | Basic and acidic residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 180 – 197 | Polar residuesSequence analysisAdd BLAST | 18 |
Domaini
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000161824 |
HOGENOMi | CLU_097977_2_0_1 |
InParanoidi | Q9QYC3 |
OMAi | HRYSTQI |
OrthoDBi | 1528350at2759 |
PhylomeDBi | Q9QYC3 |
TreeFami | TF315153 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKTKNRPPRR RTPMQDTEAT PGEQTPDRPQ SGSGGSELTK GLRSRTARAS
60 70 80 90 100
GGRGEVSRRR QGSGGRRENS VQRRLESNER ERQRMHKLNN AFQALREVIP
110 120 130 140 150
HVRADKKLSK IETLTLAKNY IKSLTATILT MSSSRLPGLE APGPAPGPKL
160 170 180 190
YQHYHHQQQQ QQQQQQVAGA MLGVTEDQPQ GHLQRYSTQI HSFREGS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 107 | Missing in AAF17706 (Ref. 2) Curated | 1 | |
Sequence conflicti | 166 | Q → QV in AAF17706 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF091858 Genomic DNA Translation: AAD51766.1 AF049660 mRNA Translation: AAF17706.1 AK020643 mRNA Translation: BAB32160.1 BC011486 mRNA Translation: AAH11486.1 |
CCDSi | CCDS19847.1 |
RefSeqi | NP_034930.1, NM_010800.4 |
Genome annotation databases
Ensembli | ENSMUST00000060747; ENSMUSP00000055493; ENSMUSG00000052271 |
GeneIDi | 17341 |
KEGGi | mmu:17341 |
UCSCi | uc009alh.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF091858 Genomic DNA Translation: AAD51766.1 AF049660 mRNA Translation: AAF17706.1 AK020643 mRNA Translation: BAB32160.1 BC011486 mRNA Translation: AAH11486.1 |
CCDSi | CCDS19847.1 |
RefSeqi | NP_034930.1, NM_010800.4 |
3D structure databases
SMRi | Q9QYC3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201426, 1 interactor |
STRINGi | 10090.ENSMUSP00000055493 |
PTM databases
iPTMneti | Q9QYC3 |
PhosphoSitePlusi | Q9QYC3 |
Proteomic databases
PaxDbi | Q9QYC3 |
PRIDEi | Q9QYC3 |
ProteomicsDBi | 265211 |
Protocols and materials databases
Antibodypediai | 30223, 123 antibodies from 25 providers |
DNASUi | 17341 |
Genome annotation databases
Ensembli | ENSMUST00000060747; ENSMUSP00000055493; ENSMUSG00000052271 |
GeneIDi | 17341 |
KEGGi | mmu:17341 |
UCSCi | uc009alh.1, mouse |
Organism-specific databases
CTDi | 168620 |
MGIi | MGI:891976, Bhlha15 |
VEuPathDBi | HostDB:ENSMUSG00000052271 |
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000161824 |
HOGENOMi | CLU_097977_2_0_1 |
InParanoidi | Q9QYC3 |
OMAi | HRYSTQI |
OrthoDBi | 1528350at2759 |
PhylomeDBi | Q9QYC3 |
TreeFami | TF315153 |
Miscellaneous databases
BioGRID-ORCSi | 17341, 2 hits in 67 CRISPR screens |
ChiTaRSi | Bhlha15, mouse |
PROi | PR:Q9QYC3 |
RNActi | Q9QYC3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000052271, Expressed in dorsal pancreas and 144 other tissues |
Genevisiblei | Q9QYC3, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BHA15_MOUSE | |
Accessioni | Q9QYC3Primary (citable) accession number: Q9QYC3 Secondary accession number(s): Q9QYE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot