Bhlha15

Functionⁱ

Plays a role in controlling the transcriptional activity of MyoD, ensuring that expanding myoblast populations remain undifferentiated (PubMed:17612490). Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'.4 Publications

GO - Molecular functionⁱ

DNA binding
Source: MGI
Inferred from direct assayⁱ
- 15024058
DNA-binding transcription activator activity, RNA polymerase II-specific
Source: NTNU_SB
Inferred from direct assayⁱ
- 15024058
protein homodimerization activity
Source: MGI
Inferred from physical interactionⁱ
- 15024058
RNA polymerase II regulatory region sequence-specific DNA binding
Source: NTNU_SB
Inferred from direct assayⁱ
- 15024058

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

calcium-mediated signaling
Source: MGI
Inferred from mutant phenotypeⁱ
- 15665001
cell-cell signaling
Source: MGI
Inferred from mutant phenotypeⁱ
- 12829745
cell maturation
Source: MGI
Inferred from mutant phenotypeⁱ
- 15024058
- 16423820
cellular response to glucose starvation
Source: UniProtKB
Inferred from direct assayⁱ
- 17612490
endoplasmic reticulum unfolded protein response
Source: UniProtKB
Inferred from direct assayⁱ
- 17612490
glucose homeostasis
Source: MGI
Inferred from mutant phenotypeⁱ
- 16423820
Golgi organization
Source: MGI
Inferred from mutant phenotypeⁱ
- 15665001
G protein-coupled receptor signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 11696558
intracellular distribution of mitochondria
Source: MGI
Inferred from mutant phenotypeⁱ
- 15665001
mitochondrial calcium ion transmembrane transport
Source: MGI
Inferred from mutant phenotypeⁱ
- 15665001
negative regulation of myotube differentiation
Source: UniProtKB
Inferred from direct assayⁱ
- 17612490
positive regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 12829745
- 15024058
regulation of transcription, DNA-templated
Source: MGI
Traceable author statementⁱ
- 9073453

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Class A basic helix-loop-helix protein 15 Short name: bHLHa15 Alternative name(s): Class B basic helix-loop-helix protein 8 Short name: bHLHb8 Muscle, intestine and stomach expression 1 Short name: MIST-1
Gene namesⁱ	Name:Bhlha15 Synonyms:Bhlhb8, Mist1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 5

Organism-specific databases

MGIⁱ	MGI:891976 Bhlha15

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mice display incorrect granule organization in pancreatic acinar cells and other serous exocrine cells such as parotid acini and gastric chief cells. They also display mislocalization of mitochondria and Golgi apparatus and reduced Ca²⁺ uptake by mitochondria.2 Publications

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127151	1 – 197	Class A basic helix-loop-helix protein 15Add BLAST		197

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	12	PhosphothreonineCombined sources		1
Modified residueⁱ	25	PhosphothreonineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q9QYC3
PRIDEⁱ	Q9QYC3

PTM databases

iPTMnetⁱ	Q9QYC3
PhosphoSitePlusⁱ	Q9QYC3

Expressionⁱ

Tissue specificityⁱ

Expressed in pancreatic tissue only in acinar cells. There is a complete absence of expression in intra- or interlobular pancreatic ducts and in all islet cells.1 Publication

Developmental stageⁱ

First observed at E10.5 in the primitive gut and in the developing lung bud. Expression in the gut persists through E16.5 and remains restricted primarily to the epithelial lining of the esophagus, stomach and intestine. Expression in the lung is detected in the bronchial epithelium at E14.5 and at E15.5. Expressed specifically in acinar cells during pancreatic development. Detected in skeletal muscle tissues beginning at E12.5, persisting throughout all embryonic stages examined although, in older embryos expression becomes severely reduced.1 Publication

Inductionⁱ

Up-regulated by XBP1. Induced by chemical activators of the unfolded protein response (UPR) such as tunicamycin and thapsigargin, and also by glucose starvation (PubMed:17612490).

Gene expression databases

Bgeeⁱ	ENSMUSG00000052271 Expressed in 115 organ(s), highest expression level in dorsal pancreas
Genevisibleⁱ	Q9QYC3 MM

Interactionⁱ

Subunit structureⁱ

Forms homodimers or heterodimers with TCF3 gene products E12 and E47. These dimers bind to the E-box site, however, heterodimer with MYOD1 does not bind target DNA.

GO - Molecular functionⁱ

protein homodimerization activity
Source: MGI
Inferred from physical interactionⁱ
- 15024058

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	201426, 1 interactor

BioGridⁱ

201426, 1 interactor

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9QYC3
SMRⁱ	Q9QYC3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	72 – 124	bHLHPROSITE-ProRule annotationAdd BLAST		53

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	152 – 189	Gln-richAdd BLAST		38

Domainⁱ

Lacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators.

Phylogenomic databases

eggNOGⁱ	ENOG410KD2P Eukaryota ENOG4111ZMK LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00680000099860
HOGENOMⁱ	HOG000095227
InParanoidⁱ	Q9QYC3
KEGG Orthology (KO) More...KOⁱ	K08040
OMAⁱ	HRYSTQI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G15AI
PhylomeDBⁱ	Q9QYC3
TreeFamⁱ	TF315153

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9QYC3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKTKNRPPRR RTPMQDTEAT PGEQTPDRPQ SGSGGSELTK GLRSRTARAS 
        60         70         80         90        100
GGRGEVSRRR QGSGGRRENS VQRRLESNER ERQRMHKLNN AFQALREVIP 
       110        120        130        140        150
HVRADKKLSK IETLTLAKNY IKSLTATILT MSSSRLPGLE APGPAPGPKL 
       160        170        180        190 
YQHYHHQQQQ QQQQQQVAGA MLGVTEDQPQ GHLQRYSTQI HSFREGS

Length:197

Mass (Da):22,150

Last modified:May 1, 2000 - v1

Checksum:ⁱBB34F1EE76FEE787

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	107	Missing in AAF17706 (Ref. 2) Curated		1
Sequence conflictⁱ	166	Q → QV in AAF17706 (Ref. 2) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF091858 Genomic DNA Translation: AAD51766.1 AF049660 mRNA Translation: AAF17706.1 AK020643 mRNA Translation: BAB32160.1 BC011486 mRNA Translation: AAH11486.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS19847.1
NCBI Reference Sequences More...RefSeqⁱ	NP_034930.1, NM_010800.4
UniGeneⁱ	Mm.386767

Genome annotation databases

Ensemblⁱ	ENSMUST00000060747; ENSMUSP00000055493; ENSMUSG00000052271
GeneIDⁱ	17341
KEGGⁱ	mmu:17341
UCSC genome browser More...UCSCⁱ	uc009alh.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9QYC3	Class A basic helix-loop-helix protein 15		RAT		197	UniRef90_Q9QYC3
Class A basic helix-loop-helix protein 15		CRIGR		197
Basic helix-loop-helix family, member a15		MOUSE		197
UPI000A309A1A		MUSPA		198
UPI000A316C01		MUSCR		198
+5

Protein	Similar proteins		Species	Score	Length	Source
Q9QYC3	Class A basic helix-loop-helix protein 15		RAT		197	UniRef50_Q9QYC3
Class A basic helix-loop-helix protein 15		CRIGR		197
Basic helix-loop-helix family, member a15		MOUSE		197
Class A basic helix-loop-helix protein 15		HETGA		193
Uncharacterized protein		NEOLE		239
+19

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF091858 Genomic DNA Translation: AAD51766.1 AF049660 mRNA Translation: AAF17706.1 AK020643 mRNA Translation: BAB32160.1 BC011486 mRNA Translation: AAH11486.1
CCDSⁱ	CCDS19847.1
RefSeqⁱ	NP_034930.1, NM_010800.4
UniGeneⁱ	Mm.386767

3D structure databases

ProteinModelPortalⁱ	Q9QYC3
SMRⁱ	Q9QYC3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	201426, 1 interactor

BioGridⁱ

201426, 1 interactor

PTM databases

iPTMnetⁱ	Q9QYC3
PhosphoSitePlusⁱ	Q9QYC3

Proteomic databases

PaxDbⁱ	Q9QYC3
PRIDEⁱ	Q9QYC3

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000060747; ENSMUSP00000055493; ENSMUSG00000052271
GeneIDⁱ	17341
KEGGⁱ	mmu:17341
UCSCⁱ	uc009alh.1 mouse

Organism-specific databases

CTDⁱ	168620
MGIⁱ	MGI:891976 Bhlha15

Phylogenomic databases

eggNOGⁱ	ENOG410KD2P Eukaryota ENOG4111ZMK LUCA
GeneTreeⁱ	ENSGT00680000099860
HOGENOMⁱ	HOG000095227
InParanoidⁱ	Q9QYC3
KOⁱ	K08040
OMAⁱ	HRYSTQI
OrthoDBⁱ	EOG091G15AI
PhylomeDBⁱ	Q9QYC3
TreeFamⁱ	TF315153

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9QYC3
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000052271 Expressed in 115 organ(s), highest expression level in dorsal pancreas
Genevisibleⁱ	Q9QYC3 MM

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfamⁱ	View protein in Pfam PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BHA15_MOUSE
Accessionⁱ	Q9QYC3Primary (citable) accession number: Q9QYC3 Secondary accession number(s): Q9QYE4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
	Last sequence update:	May 1, 2000
	Last modified:	November 7, 2018
	This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9QYC3 (BHA15_MOUSE)

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Class A basic helix-loop-helix protein 15

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Compositional bias

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ