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Protein

Class A basic helix-loop-helix protein 15

Gene

Bhlha15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in controlling the transcriptional activity of MyoD, ensuring that expanding myoblast populations remain undifferentiated (PubMed:17612490). Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Class A basic helix-loop-helix protein 15
Short name:
bHLHa15
Alternative name(s):
Class B basic helix-loop-helix protein 8
Short name:
bHLHb8
Muscle, intestine and stomach expression 1
Short name:
MIST-1
Gene namesi
Name:Bhlha15
Synonyms:Bhlhb8, Mist1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:891976 Bhlha15

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display incorrect granule organization in pancreatic acinar cells and other serous exocrine cells such as parotid acini and gastric chief cells. They also display mislocalization of mitochondria and Golgi apparatus and reduced Ca2+ uptake by mitochondria.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271511 – 197Class A basic helix-loop-helix protein 15Add BLAST197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphothreonineCombined sources1
Modified residuei25PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QYC3
PRIDEiQ9QYC3

PTM databases

iPTMnetiQ9QYC3
PhosphoSitePlusiQ9QYC3

Expressioni

Tissue specificityi

Expressed in pancreatic tissue only in acinar cells. There is a complete absence of expression in intra- or interlobular pancreatic ducts and in all islet cells.1 Publication

Developmental stagei

First observed at E10.5 in the primitive gut and in the developing lung bud. Expression in the gut persists through E16.5 and remains restricted primarily to the epithelial lining of the esophagus, stomach and intestine. Expression in the lung is detected in the bronchial epithelium at E14.5 and at E15.5. Expressed specifically in acinar cells during pancreatic development. Detected in skeletal muscle tissues beginning at E12.5, persisting throughout all embryonic stages examined although, in older embryos expression becomes severely reduced.1 Publication

Inductioni

Up-regulated by XBP1. Induced by chemical activators of the unfolded protein response (UPR) such as tunicamycin and thapsigargin, and also by glucose starvation (PubMed:17612490).

Gene expression databases

BgeeiENSMUSG00000052271 Expressed in 115 organ(s), highest expression level in dorsal pancreas
GenevisibleiQ9QYC3 MM

Interactioni

Subunit structurei

Forms homodimers or heterodimers with TCF3 gene products E12 and E47. These dimers bind to the E-box site, however, heterodimer with MYOD1 does not bind target DNA.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201426, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliQ9QYC3
SMRiQ9QYC3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 124bHLHPROSITE-ProRule annotationAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi152 – 189Gln-richAdd BLAST38

Domaini

Lacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators.

Phylogenomic databases

eggNOGiENOG410KD2P Eukaryota
ENOG4111ZMK LUCA
GeneTreeiENSGT00680000099860
HOGENOMiHOG000095227
InParanoidiQ9QYC3
KOiK08040
OMAiHRYSTQI
OrthoDBiEOG091G15AI
PhylomeDBiQ9QYC3
TreeFamiTF315153

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QYC3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKTKNRPPRR RTPMQDTEAT PGEQTPDRPQ SGSGGSELTK GLRSRTARAS
60 70 80 90 100
GGRGEVSRRR QGSGGRRENS VQRRLESNER ERQRMHKLNN AFQALREVIP
110 120 130 140 150
HVRADKKLSK IETLTLAKNY IKSLTATILT MSSSRLPGLE APGPAPGPKL
160 170 180 190
YQHYHHQQQQ QQQQQQVAGA MLGVTEDQPQ GHLQRYSTQI HSFREGS
Length:197
Mass (Da):22,150
Last modified:May 1, 2000 - v1
Checksum:iBB34F1EE76FEE787
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107Missing in AAF17706 (Ref. 2) Curated1
Sequence conflicti166Q → QV in AAF17706 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091858 Genomic DNA Translation: AAD51766.1
AF049660 mRNA Translation: AAF17706.1
AK020643 mRNA Translation: BAB32160.1
BC011486 mRNA Translation: AAH11486.1
CCDSiCCDS19847.1
RefSeqiNP_034930.1, NM_010800.4
UniGeneiMm.386767

Genome annotation databases

EnsembliENSMUST00000060747; ENSMUSP00000055493; ENSMUSG00000052271
GeneIDi17341
KEGGimmu:17341
UCSCiuc009alh.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091858 Genomic DNA Translation: AAD51766.1
AF049660 mRNA Translation: AAF17706.1
AK020643 mRNA Translation: BAB32160.1
BC011486 mRNA Translation: AAH11486.1
CCDSiCCDS19847.1
RefSeqiNP_034930.1, NM_010800.4
UniGeneiMm.386767

3D structure databases

ProteinModelPortaliQ9QYC3
SMRiQ9QYC3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201426, 1 interactor

PTM databases

iPTMnetiQ9QYC3
PhosphoSitePlusiQ9QYC3

Proteomic databases

PaxDbiQ9QYC3
PRIDEiQ9QYC3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060747; ENSMUSP00000055493; ENSMUSG00000052271
GeneIDi17341
KEGGimmu:17341
UCSCiuc009alh.1 mouse

Organism-specific databases

CTDi168620
MGIiMGI:891976 Bhlha15

Phylogenomic databases

eggNOGiENOG410KD2P Eukaryota
ENOG4111ZMK LUCA
GeneTreeiENSGT00680000099860
HOGENOMiHOG000095227
InParanoidiQ9QYC3
KOiK08040
OMAiHRYSTQI
OrthoDBiEOG091G15AI
PhylomeDBiQ9QYC3
TreeFamiTF315153

Miscellaneous databases

PROiPR:Q9QYC3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052271 Expressed in 115 organ(s), highest expression level in dorsal pancreas
GenevisibleiQ9QYC3 MM

Family and domain databases

CDDicd00083 HLH, 1 hit
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
PfamiView protein in Pfam
PF00010 HLH, 1 hit
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SUPFAMiSSF47459 SSF47459, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBHA15_MOUSE
AccessioniPrimary (citable) accession number: Q9QYC3
Secondary accession number(s): Q9QYE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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