UniProtKB - Q9QYC0 (ADDA_MOUSE)
Protein
Alpha-adducin
Gene
Add1
Organism
Mus musculus (Mouse)
Status
Functioni
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
GO - Molecular functioni
- actin filament binding Source: MGI
- calmodulin binding Source: UniProtKB-KW
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- spectrin binding Source: MGI
- structural constituent of cytoskeleton Source: MGI
- T cell receptor binding Source: MGI
- transcription factor binding Source: MGI
GO - Biological processi
- actin filament bundle assembly Source: MGI
- barbed-end actin filament capping Source: MGI
- cell morphogenesis Source: MGI
- cellular response to calcium ion Source: CAFA
- cellular response to retinoic acid Source: MGI
- cell volume homeostasis Source: MGI
- erythrocyte differentiation Source: MGI
- hemoglobin metabolic process Source: MGI
- homeostasis of number of cells within a tissue Source: MGI
- in utero embryonic development Source: MGI
- multicellular organism growth Source: MGI
- negative regulation of actin filament polymerization Source: MGI
- positive regulation of adherens junction organization Source: CAFA
- positive regulation of angiogenesis Source: MGI
- positive regulation of endocytosis Source: MGI
- positive regulation of establishment of endothelial barrier Source: CAFA
- positive regulation of protein binding Source: MGI
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding |
Enzyme and pathway databases
Reactomei | R-MMU-264870 Caspase-mediated cleavage of cytoskeletal proteins R-MMU-5223345 Miscellaneous transport and binding events |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-adducinAlternative name(s): Erythrocyte adducin subunit alpha |
Gene namesi | Name:Add1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:87918 Add1 |
Subcellular locationi
Plasma membrane
Cytoskeleton
Cytoskeleton
- cytoskeleton Source: MGI
- F-actin capping protein complex Source: MGI
Nucleus
- nuclear body Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
- plasma membrane raft Source: GO_Central
Other locations
- cell Source: GOC
- cell-cell adherens junction Source: CAFA
- cytoplasm Source: MGI
- dendrite Source: MGI
- dendritic spine Source: MGI
- membrane Source: MGI
- perinuclear region of cytoplasm Source: MGI
- postsynaptic density Source: MGI
- synapse Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218531 | 1 – 735 | Alpha-adducinAdd BLAST | 735 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | ||
Modified residuei | 12 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 59 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 64 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 331 | PhosphothreonineBy similarity | 1 | ||
Modified residuei | 334 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 353 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 355 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 358 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 364 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 366 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 408 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 427 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 429 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 431 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 436 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 445 | Phosphothreonine; by ROCK2By similarity | 1 | ||
Modified residuei | 464 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 465 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 480 | Phosphothreonine; by ROCK2By similarity | 1 | ||
Modified residuei | 481 | Phosphoserine; by PKABy similarity | 1 | ||
Modified residuei | 586 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 600 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 605 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 610 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 613 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 614 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 705 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 708 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 712 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 714 | Phosphoserine; by PKCBy similarity | 1 | ||
Modified residuei | 724 | Phosphoserine; by PKA and PKCBy similarity | 1 | ||
Isoform 2 (identifier: Q9QYC0-2) | |||||
Modified residuei | 600 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 605 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 610 | PhosphothreonineCombined sources | 1 | ||
Modified residuei | 613 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 614 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9QYC0 |
jPOSTi | Q9QYC0 |
PaxDbi | Q9QYC0 |
PeptideAtlasi | Q9QYC0 |
PRIDEi | Q9QYC0 |
PTM databases
iPTMneti | Q9QYC0 |
PhosphoSitePlusi | Q9QYC0 |
SwissPalmi | Q9QYC0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029106 Expressed in 287 organ(s), highest expression level in blood |
ExpressionAtlasi | Q9QYC0 baseline and differential |
Genevisiblei | Q9QYC0 MM |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.
GO - Molecular functioni
- actin filament binding Source: MGI
- calmodulin binding Source: UniProtKB-KW
- protein heterodimerization activity Source: MGI
- protein homodimerization activity Source: MGI
- spectrin binding Source: MGI
- T cell receptor binding Source: MGI
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGridi | 197981, 10 interactors |
IntActi | Q9QYC0, 7 interactors |
MINTi | Q9QYC0 |
STRINGi | 10090.ENSMUSP00000109979 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 715 – 732 | Interaction with calmodulinSequence analysisAdd BLAST | 18 |
Domaini
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3699 Eukaryota COG0235 LUCA |
GeneTreei | ENSGT00940000158581 |
HOGENOMi | HOG000116349 |
InParanoidi | Q9QYC0 |
KOi | K18622 |
OMAi | FMLHSAI |
PhylomeDBi | Q9QYC0 |
TreeFami | TF313003 |
Family and domain databases
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR027766 ADD1 IPR001303 Aldolase_II/adducin_N IPR036409 Aldolase_II/adducin_N_sf |
PANTHERi | PTHR10672:SF4 PTHR10672:SF4, 1 hit |
Pfami | View protein in Pfam PF00596 Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007 Aldolase_II, 1 hit |
SUPFAMi | SSF53639 SSF53639, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
This entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9QYC0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNGDTRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN
60 70 80 90 100
MMEQKKRVSM ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTA
110 120 130 140 150
SVPNVYPAAP QGGMAALNMS LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA
160 170 180 190 200
AFYRLADLFG WSQLIYNHIT TRVNSEQEHF LIVPFGLLYS EVTASSLVKV
210 220 230 240 250
NLQGDIVDRG STNLGVNQAG FTLHSAVYAA RPDAKCIVHI HTPAGAAVSA
260 270 280 290 300
MKCGLLPISP EALSLGDVAY HDYHGILVDE EEKILIQKNL GPKSKVLILR
310 320 330 340 350
NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLDPGKYK
360 370 380 390 400
AKSRSPGTPA GEGSGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL
410 420 430 440 450
RERSKKYSDV EVPASVTGHS FASDGDSGTC SPLRHSFQKQ QREKTRWLHS
460 470 480 490 500
GRGDDASEEG QNGSSPKSKT KWTKEDGHRT STSAVPNLFV PLNTNPKEVQ
510 520 530 540 550
EMRNKIREQN LQDIKTAGPQ SQVLCGVMMD RSLVQGELVT ASKAIIEKEY
560 570 580 590 600
QPHVIVSTTG PNPFNTLTDR ELEEYRREVE RKQKGSEENL DETREQKEKS
610 620 630 640 650
PPDQSAVPNT PPSTPVKLEE DLPQEPTSRD DSDATTFKPT PPDLSPDEPS
660 670 680 690 700
EALAFPAVEE EAHASPDPTQ PPAEADPEPA SAPTPGAEEV ASPATEEGSP
710 720 730
MDPGSDGSPG KSPSKKKKKF RTPSFLKKSK KKSDS
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q1K3 | E9Q1K3_MOUSE | Alpha-adducin | Add1 | 660 | Annotation score: | ||
F8WGR0 | F8WGR0_MOUSE | Alpha-adducin | Add1 | 663 | Annotation score: | ||
F8WHZ9 | F8WHZ9_MOUSE | Alpha-adducin | Add1 | 662 | Annotation score: | ||
F6RDR0 | F6RDR0_MOUSE | Alpha-adducin | Add1 | 160 | Annotation score: | ||
F6V4G5 | F6V4G5_MOUSE | Alpha-adducin | Add1 | 135 | Annotation score: | ||
A0A0J9YVI4 | A0A0J9YVI4_MOUSE | Alpha-adducin | Add1 | 248 | Annotation score: | ||
D3Z0T1 | D3Z0T1_MOUSE | Alpha-adducin | Add1 | 141 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 234 | A → T in AAF29504 (PubMed:10845937).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000177 | 621 – 632 | DLPQE…SRDDS → AGDGCAKEYLLP in isoform 2. CuratedAdd BLAST | 12 | |
Alternative sequenceiVSP_000178 | 633 – 735 | Missing in isoform 2. CuratedAdd BLAST | 103 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF096839 mRNA Translation: AAF24971.1 AF189771 mRNA Translation: AAF29504.1 |
CCDSi | CCDS19216.1 [Q9QYC0-1] CCDS39069.1 [Q9QYC0-2] |
RefSeqi | NP_001019629.2, NM_001024458.4 [Q9QYC0-1] NP_001095914.1, NM_001102444.2 NP_001318009.1, NM_001331080.1 [Q9QYC0-1] NP_001318016.1, NM_001331087.1 [Q9QYC0-2] NP_038485.1, NM_013457.4 [Q9QYC0-2] XP_017176104.1, XM_017320615.1 [Q9QYC0-2] |
Genome annotation databases
Ensembli | ENSMUST00000114338; ENSMUSP00000109977; ENSMUSG00000029106 [Q9QYC0-2] ENSMUST00000114340; ENSMUSP00000109979; ENSMUSG00000029106 [Q9QYC0-1] |
GeneIDi | 11518 |
KEGGi | mmu:11518 |
UCSCi | uc008xcp.1 mouse [Q9QYC0-2] uc008xcr.1 mouse [Q9QYC0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF096839 mRNA Translation: AAF24971.1 AF189771 mRNA Translation: AAF29504.1 |
CCDSi | CCDS19216.1 [Q9QYC0-1] CCDS39069.1 [Q9QYC0-2] |
RefSeqi | NP_001019629.2, NM_001024458.4 [Q9QYC0-1] NP_001095914.1, NM_001102444.2 NP_001318009.1, NM_001331080.1 [Q9QYC0-1] NP_001318016.1, NM_001331087.1 [Q9QYC0-2] NP_038485.1, NM_013457.4 [Q9QYC0-2] XP_017176104.1, XM_017320615.1 [Q9QYC0-2] |
3D structure databases
SMRi | Q9QYC0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 197981, 10 interactors |
IntActi | Q9QYC0, 7 interactors |
MINTi | Q9QYC0 |
STRINGi | 10090.ENSMUSP00000109979 |
PTM databases
iPTMneti | Q9QYC0 |
PhosphoSitePlusi | Q9QYC0 |
SwissPalmi | Q9QYC0 |
Proteomic databases
EPDi | Q9QYC0 |
jPOSTi | Q9QYC0 |
PaxDbi | Q9QYC0 |
PeptideAtlasi | Q9QYC0 |
PRIDEi | Q9QYC0 |
Genome annotation databases
Ensembli | ENSMUST00000114338; ENSMUSP00000109977; ENSMUSG00000029106 [Q9QYC0-2] ENSMUST00000114340; ENSMUSP00000109979; ENSMUSG00000029106 [Q9QYC0-1] |
GeneIDi | 11518 |
KEGGi | mmu:11518 |
UCSCi | uc008xcp.1 mouse [Q9QYC0-2] uc008xcr.1 mouse [Q9QYC0-1] |
Organism-specific databases
CTDi | 118 |
MGIi | MGI:87918 Add1 |
Phylogenomic databases
eggNOGi | KOG3699 Eukaryota COG0235 LUCA |
GeneTreei | ENSGT00940000158581 |
HOGENOMi | HOG000116349 |
InParanoidi | Q9QYC0 |
KOi | K18622 |
OMAi | FMLHSAI |
PhylomeDBi | Q9QYC0 |
TreeFami | TF313003 |
Enzyme and pathway databases
Reactomei | R-MMU-264870 Caspase-mediated cleavage of cytoskeletal proteins R-MMU-5223345 Miscellaneous transport and binding events |
Miscellaneous databases
ChiTaRSi | Add1 mouse |
PROi | PR:Q9QYC0 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029106 Expressed in 287 organ(s), highest expression level in blood |
ExpressionAtlasi | Q9QYC0 baseline and differential |
Genevisiblei | Q9QYC0 MM |
Family and domain databases
Gene3Di | 3.40.225.10, 1 hit |
InterProi | View protein in InterPro IPR027766 ADD1 IPR001303 Aldolase_II/adducin_N IPR036409 Aldolase_II/adducin_N_sf |
PANTHERi | PTHR10672:SF4 PTHR10672:SF4, 1 hit |
Pfami | View protein in Pfam PF00596 Aldolase_II, 1 hit |
SMARTi | View protein in SMART SM01007 Aldolase_II, 1 hit |
SUPFAMi | SSF53639 SSF53639, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADDA_MOUSE | |
Accessioni | Q9QYC0Primary (citable) accession number: Q9QYC0 Secondary accession number(s): Q9JLE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | November 13, 2019 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot