UniProtKB - Q9QYB2 (DACH1_MOUSE)
Protein
Dachshund homolog 1
Gene
Dach1
Organism
Mus musculus (Mouse)
Status
Functioni
Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1 (By similarity). Binds to chromatin DNA via its DACHbox-N domain.By similarity3 Publications
GO - Molecular functioni
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
GO - Biological processi
- cell population proliferation Source: MGI
- development of primary female sexual characteristics Source: MGI
- negative regulation of cell migration Source: MGI
- negative regulation of cell proliferation involved in contact inhibition Source: MGI
- negative regulation of DNA biosynthetic process Source: MGI
- negative regulation of fibroblast proliferation Source: MGI
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by competitive promoter binding Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of nuclear cell cycle DNA replication Source: CACAO
- regulation of transcription, DNA-templated Source: MGI
- respiratory gaseous exchange by respiratory system Source: MGI
- suckling behavior Source: MGI
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Dachshund homolog 1Short name: Dach1 |
Gene namesi | Name:Dach1 Synonyms:Dach |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1277991, Dach1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095598 | 1 – 751 | Dachshund homolog 1Add BLAST | 751 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 484 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9QYB2 |
PRIDEi | Q9QYB2 |
PTM databases
iPTMneti | Q9QYB2 |
PhosphoSitePlusi | Q9QYB2 |
Expressioni
Tissue specificityi
Expressed at higher levels in adult kidney and lung, and at lower levels in brain and testis. Expressed in embryonal kidneys, eyes, cochleae and limb buds.2 Publications
Developmental stagei
Highest expression was found at 11.5 dpc, and expression rapidly declines at later stages of the development. Between 8.0 and 13.5 dpc is found in neural crest cells during their pre-migratory, migratory, and, in some cases post-migratory phase throughout the body axis. Also found in lateral mesenchyme of trunk and head. At 10 and 12 dpc found in mesonephric tubules. At 16 dpc found in epithalial cells of metanephric 'comma' and 'S'-shaped bodies, in mesenchymal cells of the medulla and the cortex. Colocalized to the nucleus of glomerular podocytes and epithelial cells lining many of the convoluted tubes. At 10.5 dpc found in both the anterior and posterior of the limb bud. At 11.5 dpc expression becomes increasingly peripheral, extending around the entire handplate in the mesenchymal cells underlying the apical ectodermal ridge. At 12.5 dpc expression is entirely peripheral an by 13.5 dpc is localized to the mesenchyme at the distal tips of the digits. At 10 dpc found in cells of the optic cup and in some cells surrounding the eye. At 12 dpc found in developing lens fibers, the ectoderm overlaying the developing eye and less intensely in the retina. At 12 dpc found in cells of the dorsomedial and dorsolateral epithelium of the otic vesicle. At later stages expressed in only a few specialized cell types of the cochlear duct, including the inner and outer hair cells, stria vascularis, and the mesenchymal cells directly underlying the organ of Corti. From 10.5 to 12.5 dpc expressed in the telencephalon including the olfactory bulbs, throughout the length of the neural tube and within the dorsal root ganglia, in cranial ganglia in the trigeminal ganglion and the glossopharyngeal-vagal ganglion complex. At 10.5 and 11.5 dpc expressed in punctate pattern on the ventral side of the embryo between the fore and hind limbs, the rib primordia; this expression disappears by 12.5 dpc. At 11.5 and 12.5 dpc found in genital eminence.4 Publications
Gene expression databases
Bgeei | ENSMUSG00000055639, Expressed in telencephalon and 320 other tissues |
Genevisiblei | Q9QYB2, MM |
Interactioni
Subunit structurei
Interacts with SIX1, SIX6 and EYA3.
Interacts with NCOR1 and HDAC3 through its N-terminus.
Interacts with SIN3A through its C-terminus.
Interacts with SMAD3 and SMAD4 (By similarity).
By similarityBinary interactionsi
Q9QYB2
With | #Exp. | IntAct |
---|---|---|
Ncor1 [Q60974] | 2 | EBI-348961,EBI-349004 |
Protein-protein interaction databases
BioGRIDi | 199044, 8 interactors |
CORUMi | Q9QYB2 |
IntActi | Q9QYB2, 6 interactors |
MINTi | Q9QYB2 |
STRINGi | 10090.ENSMUSP00000071464 |
Miscellaneous databases
RNActi | Q9QYB2, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 182 – 377 | Interaction with SIX6 and HDAC31 PublicationAdd BLAST | 196 | |
Regioni | 182 – 268 | DACHbox-NAdd BLAST | 87 | |
Regioni | 609 – 689 | DACHbox-CAdd BLAST | 81 | |
Regioni | 620 – 699 | Interaction with SIN3A1 PublicationAdd BLAST | 80 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 623 – 711 | Sequence analysisAdd BLAST | 89 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 76 – 96 | Poly-GlyAdd BLAST | 21 | |
Compositional biasi | 115 – 118 | Poly-Gly | 4 | |
Compositional biasi | 130 – 156 | Poly-SerAdd BLAST | 27 | |
Compositional biasi | 318 – 326 | Poly-Ala | 9 | |
Compositional biasi | 460 – 463 | Poly-Ala | 4 |
Domaini
The DACHbox-N/DD1 domain forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain.By similarity
Sequence similaritiesi
Belongs to the DACH/dachshund family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3915, Eukaryota |
GeneTreei | ENSGT00390000001134 |
HOGENOMi | CLU_027923_0_0_1 |
InParanoidi | Q9QYB2 |
OMAi | VSHPLNH |
PhylomeDBi | Q9QYB2 |
TreeFami | TF316697 |
Family and domain databases
Gene3Di | 3.10.260.20, 1 hit |
InterProi | View protein in InterPro IPR009061, DNA-bd_dom_put_sf IPR003380, SKI/SNO/DAC IPR037000, Ski_DNA-bd_sf |
Pfami | View protein in Pfam PF02437, Ski_Sno, 1 hit |
SUPFAMi | SSF46955, SSF46955, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9QYB2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVPAALIPP TQLVPPQPPI STSASSSGTT TSTSSATSSP APSIGPPASS
60 70 80 90 100
GPTLFRPEPI ASSASSSAAA TVTSPGGGGG GSGGGGGSGG NGGGGGSNCN
110 120 130 140 150
PSLAAGSSGG GVSAGGGGAS STPITASTGS SSSSSSSSSS SSSSSSSSSS
160 170 180 190 200
SSSSSSCGPL PGKPVYSTPS PVENTPQNNE CKMVDLRGAK VASFTVEGCE
210 220 230 240 250
LICLPQAFDL FLKHLVGGLH TVYTKLKRLE ITPVVCNVEQ VRILRGLGAI
260 270 280 290 300
QPGVNRCKLI SRKDFETLYN DCTNASSRPG RPPKRTQSVT SPENSHIMPH
310 320 330 340 350
SVPGLMSPGI IPPTGLTAAA AAAAAATNAA IAEAMKVKKI KLEAMSNYHA
360 370 380 390 400
SNNQHGADSE NGDMNSSVGS SGGSWDKETL HSPPSQGSQA PVAHARMPAA
410 420 430 440 450
FSLPVSHPLN HLQHSHLPPN GLELPFMMMP HPLIPVSLPP ASVTMAMSQM
460 470 480 490 500
NHLSTIANMA AAAQVQSPPS RVETSVIKER VPDSPSPAPS LEEGRRPGSH
510 520 530 540 550
PSSHRSSSVS SSPARTESSS DRIPVHQNGL SMNQMLMGLS PNVLPGPKEG
560 570 580 590 600
DLAGHDMGHE SKRIHIEKDE TPLSTPTARD SIDKLSLTGH GQPLPPGFPS
610 620 630 640 650
PFLFPDGLSS IETLLTNIQG LLKVAIDNAR AQEKQVQLEK TELKMDFLRE
660 670 680 690 700
RELRETLEKQ LAMEQKNRAI VQKRLKKEKK AKRKLQEALE FETKRREQAE
710 720 730 740 750
QTLKQAASAD SLRVLNDSLT PEIEADRSGG RADAERTIQD GRLYLKTTVM
Y
Sequence cautioni
The sequence CAA06665 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 62 | S → Y in AAD16097 (PubMed:9915577).Curated | 1 | |
Sequence conflicti | 62 | S → Y in AAD16098 (PubMed:9915577).Curated | 1 | |
Sequence conflicti | 227 | K → E in BAC33046 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 393 | A → T in CAA06665 (PubMed:9915577).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009489 | 370 – 421 | Missing in isoform 2. 4 PublicationsAdd BLAST | 52 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF102547 mRNA Translation: AAF04742.1 AF090436 mRNA Translation: AAD16097.1 AF090437 mRNA Translation: AAD16098.1 AF129510 mRNA Translation: AAF22955.1 BC141130 mRNA Translation: AAI41131.1 AK053594 mRNA Translation: BAC35441.1 AK047409 mRNA Translation: BAC33046.1 AJ005669 mRNA Translation: CAA06665.1 Different initiation. |
CCDSi | CCDS36993.1 [Q9QYB2-2] CCDS36994.1 [Q9QYB2-1] |
RefSeqi | NP_001033699.1, NM_001038610.2 [Q9QYB2-2] NP_031852.1, NM_007826.3 [Q9QYB2-1] |
Genome annotation databases
Ensembli | ENSMUST00000069334; ENSMUSP00000064970; ENSMUSG00000055639 [Q9QYB2-2] ENSMUST00000071533; ENSMUSP00000071464; ENSMUSG00000055639 [Q9QYB2-1] |
GeneIDi | 13134 |
KEGGi | mmu:13134 |
UCSCi | uc007uur.2, mouse [Q9QYB2-1] uc007uus.2, mouse [Q9QYB2-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF102547 mRNA Translation: AAF04742.1 AF090436 mRNA Translation: AAD16097.1 AF090437 mRNA Translation: AAD16098.1 AF129510 mRNA Translation: AAF22955.1 BC141130 mRNA Translation: AAI41131.1 AK053594 mRNA Translation: BAC35441.1 AK047409 mRNA Translation: BAC33046.1 AJ005669 mRNA Translation: CAA06665.1 Different initiation. |
CCDSi | CCDS36993.1 [Q9QYB2-2] CCDS36994.1 [Q9QYB2-1] |
RefSeqi | NP_001033699.1, NM_001038610.2 [Q9QYB2-2] NP_031852.1, NM_007826.3 [Q9QYB2-1] |
3D structure databases
SMRi | Q9QYB2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199044, 8 interactors |
CORUMi | Q9QYB2 |
IntActi | Q9QYB2, 6 interactors |
MINTi | Q9QYB2 |
STRINGi | 10090.ENSMUSP00000071464 |
PTM databases
iPTMneti | Q9QYB2 |
PhosphoSitePlusi | Q9QYB2 |
Proteomic databases
PaxDbi | Q9QYB2 |
PRIDEi | Q9QYB2 |
Protocols and materials databases
Antibodypediai | 72801, 283 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000069334; ENSMUSP00000064970; ENSMUSG00000055639 [Q9QYB2-2] ENSMUST00000071533; ENSMUSP00000071464; ENSMUSG00000055639 [Q9QYB2-1] |
GeneIDi | 13134 |
KEGGi | mmu:13134 |
UCSCi | uc007uur.2, mouse [Q9QYB2-1] uc007uus.2, mouse [Q9QYB2-2] |
Organism-specific databases
CTDi | 1602 |
MGIi | MGI:1277991, Dach1 |
Phylogenomic databases
eggNOGi | KOG3915, Eukaryota |
GeneTreei | ENSGT00390000001134 |
HOGENOMi | CLU_027923_0_0_1 |
InParanoidi | Q9QYB2 |
OMAi | VSHPLNH |
PhylomeDBi | Q9QYB2 |
TreeFami | TF316697 |
Miscellaneous databases
BioGRID-ORCSi | 13134, 0 hits in 17 CRISPR screens |
ChiTaRSi | Dach1, mouse |
PROi | PR:Q9QYB2 |
RNActi | Q9QYB2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000055639, Expressed in telencephalon and 320 other tissues |
Genevisiblei | Q9QYB2, MM |
Family and domain databases
Gene3Di | 3.10.260.20, 1 hit |
InterProi | View protein in InterPro IPR009061, DNA-bd_dom_put_sf IPR003380, SKI/SNO/DAC IPR037000, Ski_DNA-bd_sf |
Pfami | View protein in Pfam PF02437, Ski_Sno, 1 hit |
SUPFAMi | SSF46955, SSF46955, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DACH1_MOUSE | |
Accessioni | Q9QYB2Primary (citable) accession number: Q9QYB2 Secondary accession number(s): B2RUG1 Q9Z0Y5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2004 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families