UniProtKB - Q9QY30 (ABCBB_MOUSE)
Bile salt export pump
Abcb11
Functioni
Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed:14570929, PubMed:11172067, PubMed:23764895, PubMed:22619174, PubMed:19228692).
Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (By similarity).
Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (By similarity).
By similarity5 PublicationsCatalytic activityi
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- ATP + glycochenodeoxycholate(in) + H2O = ADP + glycochenodeoxycholate(out) + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H2O + taurochenodeoxycholate(in) = ADP + H+ + phosphate + taurochenodeoxycholate(out)By similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + glycoursodeoxycholate(in) + H2O = ADP + glycoursodeoxycholate(out) + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H2O + tauroursodeoxycholate(in) = ADP + H+ + phosphate + tauroursodeoxycholate(out)1 PublicationThis reaction proceeds in the forward1 Publication direction.
- ATP + H2O + taurolithocholate 3-sulfate(in) = ADP + H+ + phosphate + taurolithocholate 3-sulfate(out)By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 455 – 462 | ATP 1PROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 1113 – 1120 | ATP 2PROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ABC-type bile acid transporter activity Source: UniProtKB
- ABC-type xenobiotic transporter activity Source: UniProtKB
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- bile acid transmembrane transporter activity Source: UniProtKB
- canalicular bile acid transmembrane transporter activity Source: UniProtKB
- carbohydrate transmembrane transporter activity Source: MGI
GO - Biological processi
- bile acid and bile salt transport Source: UniProtKB
- bile acid metabolic process Source: UniProtKB
- bile acid signaling pathway Source: Ensembl
- canalicular bile acid transport Source: UniProtKB
- cholesterol homeostasis Source: UniProtKB
- fatty acid metabolic process Source: UniProtKB
- lipid homeostasis Source: UniProtKB
- phospholipid homeostasis Source: UniProtKB
- positive regulation of bile acid secretion Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- regulation of bile acid metabolic process Source: UniProtKB
- regulation of fatty acid beta-oxidation Source: UniProtKB
- regulation of gene expression Source: Ensembl
- response to 17alpha-ethynylestradiol Source: Ensembl
- response to estrogen Source: Ensembl
- response to ethanol Source: Ensembl
- response to oxidative stress Source: Ensembl
- response to xenobiotic stimulus Source: MGI
- transmembrane transport Source: MGI
- xenobiotic export Source: UniProtKB
- xenobiotic metabolic process Source: UniProtKB
- xenobiotic transmembrane transport Source: UniProtKB
Keywordsi
Molecular function | Translocase |
Biological process | Lipid transport, Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-159418, Recycling of bile acids and salts R-MMU-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol |
Names & Taxonomyi
Protein namesi | Recommended name: Bile salt export pump1 Publication (EC:7.6.2.-1 Publication)Alternative name(s): ATP-binding cassette sub-family B member 11 Sister of P-glycoproteinBy similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351619, Abcb11 |
VEuPathDBi | HostDB:ENSMUSG00000027048 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Multi-pass membrane protein By similarity
- Cell membrane By similarity; Multi-pass membrane protein By similarity
Endosome
- Recycling endosome membrane By similarity; Multi-pass membrane protein By similarity
- Endosome By similarity
Note: Internalized at the canalicular membrane through interaction with the adapter protein complex 2 (AP-2). At steady state, localizes in the canalicular membrane but is also present in recycling endosomes. ABCB11 constantly and rapidly exchanges between the two sites through tubulo-vesicles carriers that move along microtubules. Microtubule-dependent trafficking of ABCB11 is enhanced by taurocholate and cAMP and regulated by STK11 through a PKA-mediated pathway. Trafficking of newly synthesized ABCB11 through endosomal compartment to the bile canalicular membrane is accelerated by cAMP but not by taurocholate (By similarity). Cell membrane expression is up-regulated by short- and medium-chain fatty acids (By similarity).By similarity
Endosome
- endosome Source: UniProtKB
- recycling endosome Source: UniProtKB
- recycling endosome membrane Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: MGI
- Golgi membrane Source: MGI
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- intracellular canaliculus Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- apical part of cell Source: MGI
- cell surface Source: UniProtKB
- integral component of membrane Source: GO_Central
- intercellular canaliculus Source: MGI
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 62 | CytoplasmicSequence analysisAdd BLAST | 62 | |
Transmembranei | 63 – 83 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 84 – 147 | ExtracellularSequence analysisAdd BLAST | 64 | |
Transmembranei | 148 – 168 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 169 – 215 | CytoplasmicSequence analysisAdd BLAST | 47 | |
Transmembranei | 216 – 236 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 237 – 240 | ExtracellularSequence analysis | 4 | |
Transmembranei | 241 – 261 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 262 – 319 | CytoplasmicSequence analysisAdd BLAST | 58 | |
Transmembranei | 320 – 340 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 341 – 353 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 354 – 374 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 375 – 755 | CytoplasmicSequence analysisAdd BLAST | 381 | |
Transmembranei | 756 – 776 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 777 – 794 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 795 – 815 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 816 – 869 | CytoplasmicSequence analysisAdd BLAST | 54 | |
Transmembranei | 870 – 890 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 891 – 911 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 912 – 979 | CytoplasmicSequence analysisAdd BLAST | 68 | |
Transmembranei | 980 – 1000 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1001 – 1011 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 1012 – 1032 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 1033 – 1321 | CytoplasmicSequence analysisAdd BLAST | 289 |
Keywords - Cellular componenti
Cell membrane, Endosome, MembranePathology & Biotechi
Disruption phenotypei
Chemistry databases
ChEMBLi | CHEMBL2073695 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093297 | 1 – 1321 | Bile salt export pumpAdd BLAST | 1321 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 109 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 116 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 122 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 125 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 586 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 587 | PhosphoserineBy similarity | 1 | |
Modified residuei | 692 | PhosphoserineCombined sources | 1 | |
Modified residuei | 703 | PhosphoserineBy similarity | 1 | |
Modified residuei | 706 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1321 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Glycoprotein, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q9QY30 |
MaxQBi | Q9QY30 |
PaxDbi | Q9QY30 |
PRIDEi | Q9QY30 |
ProteomicsDBi | 296470 |
PTM databases
GlyGeni | Q9QY30, 4 sites |
iPTMneti | Q9QY30 |
PhosphoSitePlusi | Q9QY30 |
SwissPalmi | Q9QY30 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000027048, Expressed in liver and 61 other tissues |
ExpressionAtlasi | Q9QY30, baseline and differential |
Genevisiblei | Q9QY30, MM |
Interactioni
Subunit structurei
Interacts with HAX1 (By similarity).
Interacts with the adapter protein complex 2 (AP-2) throught AP2A2 or AP2A1; this interaction regulates cell membrane expression of ABCB11 through its internalization in a clathrin-dependent manner and its subsequent degradation (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000099771 |
Miscellaneous databases
RNActi | Q9QY30, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 62 – 385 | ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST | 324 | |
Domaini | 420 – 656 | ABC transporter 1PROSITE-ProRule annotationAdd BLAST | 237 | |
Domaini | 755 – 1043 | ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST | 289 | |
Domaini | 1078 – 1316 | ABC transporter 2PROSITE-ProRule annotationAdd BLAST | 239 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 651 – 674 | Interaction with HAX1By similarityAdd BLAST | 24 | |
Regioni | 662 – 722 | DisorderedSequence analysisAdd BLAST | 61 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 663 – 680 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 681 – 708 | Polar residuesSequence analysisAdd BLAST | 28 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0055, Eukaryota |
GeneTreei | ENSGT00940000157564 |
HOGENOMi | CLU_000604_17_2_1 |
InParanoidi | Q9QY30 |
OMAi | LFMLPMT |
OrthoDBi | 186078at2759 |
PhylomeDBi | Q9QY30 |
TreeFami | TF105193 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR030278, BSEP IPR027417, P-loop_NTPase IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit PTHR24221:SF165, PTHR24221:SF165, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSDSVILRSV KKFGEENHAF ESDGFHNNDK KSRLQDKKKG EGARVGFFEL
60 70 80 90 100
FRFSSSKDNW LMFMGSVCAL LHGMAQPGMI IVFGILTDIF VEYDIERQEL
110 120 130 140 150
SIPEKVCMNN TIVWINSSFN QNMTNGTSCG LVDINSEVIK FSGIYAGVGV
160 170 180 190 200
AVLILGYFQI RLWVITGARQ IRKMRKFYFR RIMRMEIGWF DCTSVGELNS
210 220 230 240 250
RFSDDINKID EAIADQMALF LQRLSTALSG LLLGFYRGWK LTLVILAVSP
260 270 280 290 300
LIGIGAAVIG LSVAKFTELE LKAYAKAGSI ADEVLSSIRT VAAFGGENKE
310 320 330 340 350
VERYEKNLMF AQRWGIWKGM VMGFFTGYMW CLIFFCYALA FWYGSRLVLD
360 370 380 390 400
EGEYTPGTLI QIFLCVIIAA MNIGNASSCL EIFSTGCSAA SSIFQTIDRQ
410 420 430 440 450
PVMDCMSGDG YKLDRIKGEI EFHNVTFHYP SRPEVKILNN LSMVIKPGET
460 470 480 490 500
TAFVGSSGAG KSTALQLIQR FYDPCEGMVT LDGHDIRSLN IRWLRDQIGI
510 520 530 540 550
VEQEPVLFST TIAENIRLGR EEATMEDIVQ AAKDANAYNF IMALPQQFDT
560 570 580 590 600
LVGEGGGQMS GGQKQRVAIA RALIRKPKIL LLDMATSALD NESEAKVQGA
610 620 630 640 650
LNKIQHGHTI ISVAHRLSTV RSADVIIGFE HGTAVERGTH EELLERKGVY
660 670 680 690 700
FMLVTLQSQE DNTHKETGIK GKDTTEGDTP ERTFSRGSYQ DSLRASIRQR
710 720 730 740 750
SKSQLSHLSH EPPLAIGDHK SSYEDRKDND VLVEEVEPAP VRRILKYNIS
760 770 780 790 800
EWPYILVGAL CAAINGAVTP IYSLLFSQIL KTFSLVDKEQ QRSEIYSMCL
810 820 830 840 850
FFVILGCVSL FTQFLQGYNF AKSGELLTKR LRKFGFKAML RQDIGWFDDL
860 870 880 890 900
KNNPGVLTTR LATDASQVQG ATGSQVGMMV NSFTNIFVAV LIAFLFNWKL
910 920 930 940 950
SLVISVFFPF LALSGAVQTK MLTGFASQDK EILEKAGQIT NEALSNIRTV
960 970 980 990 1000
AGIGVEGRFI KAFEVELEKS YKTAIRKANV YGLCYAFSQG ISFLANSAAY
1010 1020 1030 1040 1050
RYGGYLIVYE DLNFSYVFRV VSSIAMSATA VGRTFSYTPS YAKAKISAAR
1060 1070 1080 1090 1100
FFQLLDRKPP IDVYSGAGEK WDNFQGKIDF IDCKFTYPSR PDIQVLNGLS
1110 1120 1130 1140 1150
VSVDPGQTLA FVGSSGCGKS TSIQLLERFY DPDQGTVMID GHDSKKVNVQ
1160 1170 1180 1190 1200
FLRSNIGIVS QEPVLFDCSI MDNIKYGDNT KEISVERAIA AAKQAQLHDF
1210 1220 1230 1240 1250
VMSLPEKYET NVGIQGSQLS RGEKQRIAIA RAIVRDPKIL LLDEATSALD
1260 1270 1280 1290 1300
TESEKTVQLA LDKAREGRTC IVIAHRLSTI QNSDIIAVMS QGVVIEKGTH
1310 1320
KKLMDQKGAY YKLVITGAPI S
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketJ3QNY6 | J3QNY6_MOUSE | Bile salt export pump | Abcb11 | 1,321 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 104 | E → G in AAF14372 (PubMed:10607905).Curated | 1 | |
Sequence conflicti | 481 | L → P in AAD56419 (Ref. 3) Curated | 1 | |
Sequence conflicti | 633 | T → V in AAD56419 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF133903 mRNA Translation: AAF14372.1 AL929170 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL27032.1 AF186585 mRNA Translation: AAD56419.1 |
CCDSi | CCDS16090.1 |
RefSeqi | NP_066302.2, NM_021022.3 XP_006499732.1, XM_006499669.3 |
Genome annotation databases
Ensembli | ENSMUST00000102709; ENSMUSP00000099770; ENSMUSG00000027048 ENSMUST00000102710; ENSMUSP00000099771; ENSMUSG00000027048 |
GeneIDi | 27413 |
KEGGi | mmu:27413 |
UCSCi | uc008jya.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF133903 mRNA Translation: AAF14372.1 AL929170 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL27032.1 AF186585 mRNA Translation: AAD56419.1 |
CCDSi | CCDS16090.1 |
RefSeqi | NP_066302.2, NM_021022.3 XP_006499732.1, XM_006499669.3 |
3D structure databases
SMRi | Q9QY30 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000099771 |
Chemistry databases
ChEMBLi | CHEMBL2073695 |
PTM databases
GlyGeni | Q9QY30, 4 sites |
iPTMneti | Q9QY30 |
PhosphoSitePlusi | Q9QY30 |
SwissPalmi | Q9QY30 |
Proteomic databases
jPOSTi | Q9QY30 |
MaxQBi | Q9QY30 |
PaxDbi | Q9QY30 |
PRIDEi | Q9QY30 |
ProteomicsDBi | 296470 |
Protocols and materials databases
Antibodypediai | 1033, 245 antibodies from 33 providers |
DNASUi | 27413 |
Genome annotation databases
Ensembli | ENSMUST00000102709; ENSMUSP00000099770; ENSMUSG00000027048 ENSMUST00000102710; ENSMUSP00000099771; ENSMUSG00000027048 |
GeneIDi | 27413 |
KEGGi | mmu:27413 |
UCSCi | uc008jya.1, mouse |
Organism-specific databases
CTDi | 8647 |
MGIi | MGI:1351619, Abcb11 |
VEuPathDBi | HostDB:ENSMUSG00000027048 |
Phylogenomic databases
eggNOGi | KOG0055, Eukaryota |
GeneTreei | ENSGT00940000157564 |
HOGENOMi | CLU_000604_17_2_1 |
InParanoidi | Q9QY30 |
OMAi | LFMLPMT |
OrthoDBi | 186078at2759 |
PhylomeDBi | Q9QY30 |
TreeFami | TF105193 |
Enzyme and pathway databases
Reactomei | R-MMU-159418, Recycling of bile acids and salts R-MMU-193368, Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol |
Miscellaneous databases
BioGRID-ORCSi | 27413, 1 hit in 66 CRISPR screens |
PROi | PR:Q9QY30 |
RNActi | Q9QY30, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027048, Expressed in liver and 61 other tissues |
ExpressionAtlasi | Q9QY30, baseline and differential |
Genevisiblei | Q9QY30, MM |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like_ATP-bd IPR017871, ABC_transporter-like_CS IPR030278, BSEP IPR027417, P-loop_NTPase IPR039421, Type_1_exporter |
PANTHERi | PTHR24221, PTHR24221, 1 hit PTHR24221:SF165, PTHR24221:SF165, 1 hit |
Pfami | View protein in Pfam PF00664, ABC_membrane, 2 hits PF00005, ABC_tran, 2 hits |
SMARTi | View protein in SMART SM00382, AAA, 2 hits |
SUPFAMi | SSF52540, SSF52540, 2 hits SSF90123, SSF90123, 2 hits |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 2 hits PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ABCBB_MOUSE | |
Accessioni | Q9QY30Primary (citable) accession number: Q9QY30 Secondary accession number(s): A2AUN4, Q9QZE8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | June 28, 2011 | |
Last modified: | February 23, 2022 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families