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Protein

Protein kinase C and casein kinase substrate in neurons 2 protein

Gene

Pacsin2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.1 Publication

GO - Molecular functioni

  • cytoskeletal protein binding Source: GO_Central
  • identical protein binding Source: Ensembl
  • phosphatidic acid binding Source: UniProtKB
  • phospholipid binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-RNO-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons 2 protein
Alternative name(s):
Synaptic dynamin-associated protein II
Syndapin-2
Syndapin-II
Short name:
SdpII
Gene namesi
Name:Pacsin2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi69411 Pacsin2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001617971 – 488Protein kinase C and casein kinase substrate in neurons 2 proteinAdd BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-acetyllysineBy similarity1
Modified residuei273PhosphoserineBy similarity1
Modified residuei401PhosphoserineCombined sources1
Modified residuei448PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QY17
PRIDEiQ9QY17

PTM databases

iPTMnetiQ9QY17
PhosphoSitePlusiQ9QY17

Expressioni

Tissue specificityi

Widely expressed (at protein level). Isoforms 1/3 are predominantly expressed in heart and in PC-12 cells, a pheochromocytoma cell line (at protein level). Isoforms 2/4 are widely expressed with highest levels in muscle, testis and brain (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000009756 Expressed in 10 organ(s), highest expression level in heart
ExpressionAtlasiQ9QY17 baseline and differential
GenevisibleiQ9QY17 RN

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250783, 1 interactor
ELMiQ9QY17
IntActiQ9QY17, 5 interactors
MINTiQ9QY17
STRINGi10116.ENSRNOP00000060074

Structurei

3D structure databases

ProteinModelPortaliQ9QY17
SMRiQ9QY17
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 282F-BARPROSITE-ProRule annotationAdd BLAST272
Domaini428 – 488SH3PROSITE-ProRule annotationAdd BLAST61

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili25 – 274By similarityAdd BLAST250

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi364 – 366NPF13
Motifi407 – 409NPF23
Motifi419 – 421NPF33

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain (By similarity). Endocytic vesicle-like distribution.By similarity

Sequence similaritiesi

Belongs to the PACSIN family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2856 Eukaryota
ENOG410XRX2 LUCA
GeneTreeiENSGT00510000046376
HOGENOMiHOG000007245
HOVERGENiHBG053486
InParanoidiQ9QY17
KOiK20123
OMAiSYKNIYR
PhylomeDBiQ9QY17

Family and domain databases

CDDicd07679 F-BAR_PACSIN2, 1 hit
cd11998 SH3_PACSIN1-2, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR035743 PACSIN1/PACSIN2_SH3
IPR028521 PACSIN2
IPR037453 PACSIN2_F-BAR
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR23065:SF14 PTHR23065:SF14, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50002 SH3, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9QY17-1) [UniParc]FASTAAdd to basket
Also known as: Aa, SdpII-l

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCGD LMNCLHERAR
60 70 80 90 100
IEKAYAQQLT EWARRWRQLV EKGPQYGTVE KAWMAVMSEA ERVSELHLEV
110 120 130 140 150
KASLMNEDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK
160 170 180 190 200
EVDAAKKAHH TACKEEKLAV SREANSKADP SLNPEQLKKL QDKIEKCKQD
210 220 230 240 250
VLKTKDKYEK ALKELDQTTP QYMENMEQVF EQCQQFEEKR LRFFREVLLE
260 270 280 290 300
VQKHLDLSNV ASYKGIYREL EQSIKAADAV EDLRWFRANH GPGMAMNWPQ
310 320 330 340 350
FEDEEWSADL NRTLSRREKK KAADGVTLTG INQTGDQSGQ NKPSSNLSVP
360 370 380 390 400
SNPAQSTQLQ SSYNPFEDED DTGSSVSEKE DIKAKNVSSY EKTQNYPADW
410 420 430 440 450
SDDESNNPFS STDANGDSNP FDEDTTSGTE VRVRALYDYE GQEHDELSFK
460 470 480
AGDELTKIED EDEQGWCKGR LDSGQVGLYP ANYVEAIQ
Length:488
Mass (Da):55,978
Last modified:August 13, 2002 - v2
Checksum:iB2975012EF0FDF56
GO
Isoform 2 (identifier: Q9QY17-2) [UniParc]FASTAAdd to basket
Also known as: Ab

The sequence of this isoform differs from the canonical sequence as follows:
     346-386: Missing.

Show »
Length:447
Mass (Da):51,538
Checksum:iB202B81D7D48E6D1
GO
Isoform 3 (identifier: Q9QY17-3) [UniParc]FASTAAdd to basket
Also known as: Ba

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: Missing.

Show »
Length:486
Mass (Da):55,734
Checksum:iB140F324EF11E29B
GO
Isoform 4 (identifier: Q9QY17-4) [UniParc]FASTAAdd to basket
Also known as: Bb, SdpII-s

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: Missing.
     346-386: Missing.

Show »
Length:445
Mass (Da):51,294
Checksum:i99512F063951F4A6
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
R9PXU3R9PXU3_RAT
Protein kinase C and casein kinase ...
Pacsin2
484Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004518302 – 303Missing in isoform 3 and isoform 4. 1 Publication2
Alternative sequenceiVSP_004519346 – 386Missing in isoform 2 and isoform 4. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139492 mRNA Translation: AAF22211.1
AF139493 mRNA Translation: AAF22212.1
AF139494 mRNA Translation: AAF22213.1
AF139495 mRNA Translation: AAF22214.1
RefSeqiNP_570096.2, NM_130740.2 [Q9QY17-3]
XP_006242112.1, XM_006242050.3 [Q9QY17-1]
XP_006242113.1, XM_006242051.2 [Q9QY17-1]
XP_006242114.1, XM_006242052.3 [Q9QY17-1]
XP_006242115.1, XM_006242053.2 [Q9QY17-1]
XP_006242117.1, XM_006242055.2 [Q9QY17-3]
XP_006242120.1, XM_006242058.2 [Q9QY17-2]
XP_006242122.1, XM_006242060.2 [Q9QY17-4]
XP_017450116.1, XM_017594627.1 [Q9QY17-1]
UniGeneiRn.17106

Genome annotation databases

EnsembliENSRNOT00000013398; ENSRNOP00000013398; ENSRNOG00000009756 [Q9QY17-3]
ENSRNOT00000067913; ENSRNOP00000060074; ENSRNOG00000009756 [Q9QY17-1]
ENSRNOT00000088125; ENSRNOP00000072822; ENSRNOG00000009756 [Q9QY17-3]
GeneIDi124461
KEGGirno:124461

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139492 mRNA Translation: AAF22211.1
AF139493 mRNA Translation: AAF22212.1
AF139494 mRNA Translation: AAF22213.1
AF139495 mRNA Translation: AAF22214.1
RefSeqiNP_570096.2, NM_130740.2 [Q9QY17-3]
XP_006242112.1, XM_006242050.3 [Q9QY17-1]
XP_006242113.1, XM_006242051.2 [Q9QY17-1]
XP_006242114.1, XM_006242052.3 [Q9QY17-1]
XP_006242115.1, XM_006242053.2 [Q9QY17-1]
XP_006242117.1, XM_006242055.2 [Q9QY17-3]
XP_006242120.1, XM_006242058.2 [Q9QY17-2]
XP_006242122.1, XM_006242060.2 [Q9QY17-4]
XP_017450116.1, XM_017594627.1 [Q9QY17-1]
UniGeneiRn.17106

3D structure databases

ProteinModelPortaliQ9QY17
SMRiQ9QY17
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250783, 1 interactor
ELMiQ9QY17
IntActiQ9QY17, 5 interactors
MINTiQ9QY17
STRINGi10116.ENSRNOP00000060074

PTM databases

iPTMnetiQ9QY17
PhosphoSitePlusiQ9QY17

Proteomic databases

PaxDbiQ9QY17
PRIDEiQ9QY17

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013398; ENSRNOP00000013398; ENSRNOG00000009756 [Q9QY17-3]
ENSRNOT00000067913; ENSRNOP00000060074; ENSRNOG00000009756 [Q9QY17-1]
ENSRNOT00000088125; ENSRNOP00000072822; ENSRNOG00000009756 [Q9QY17-3]
GeneIDi124461
KEGGirno:124461

Organism-specific databases

CTDi11252
RGDi69411 Pacsin2

Phylogenomic databases

eggNOGiKOG2856 Eukaryota
ENOG410XRX2 LUCA
GeneTreeiENSGT00510000046376
HOGENOMiHOG000007245
HOVERGENiHBG053486
InParanoidiQ9QY17
KOiK20123
OMAiSYKNIYR
PhylomeDBiQ9QY17

Enzyme and pathway databases

ReactomeiR-RNO-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

PROiPR:Q9QY17

Gene expression databases

BgeeiENSRNOG00000009756 Expressed in 10 organ(s), highest expression level in heart
ExpressionAtlasiQ9QY17 baseline and differential
GenevisibleiQ9QY17 RN

Family and domain databases

CDDicd07679 F-BAR_PACSIN2, 1 hit
cd11998 SH3_PACSIN1-2, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR035743 PACSIN1/PACSIN2_SH3
IPR028521 PACSIN2
IPR037453 PACSIN2_F-BAR
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR23065:SF14 PTHR23065:SF14, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPACN2_RAT
AccessioniPrimary (citable) accession number: Q9QY17
Secondary accession number(s): Q9QY18, Q9QY19, Q9QY20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: August 13, 2002
Last modified: November 7, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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