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Protein

ATP-dependent RNA helicase DDX25

Gene

Ddx25

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi143 – 150ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Helicase, Hydrolase, RNA-binding
Biological processDifferentiation, mRNA transport, Spermatogenesis, Translation regulation, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.12 5301
3.6.4.13 5301

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX25 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 25
Gonadotropin-regulated testicular RNA helicase
Gene namesi
Name:Ddx25
Synonyms:Grth
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68381 Ddx25

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi66L → A: Enhances nuclear expression; when associated with A-67 and A-70. 1 Publication1
Mutagenesisi67L → A: Enhances nuclear expression; when associated with A-66 and A-70. 1 Publication1
Mutagenesisi70L → A: Enhances nuclear expression; when associated with A-66 and A-67. 1 Publication1
Mutagenesisi103R → A: Abolishes nuclear expression. 1 Publication1
Mutagenesisi104L → A: Decreases nuclear expression; when associated with A-108 and A-109. 1 Publication1
Mutagenesisi105K → A: Abolishes nuclear expression. 1 Publication1
Mutagenesisi108L → A: Decreases nuclear expression; when associated with A-104 and A-109. 1 Publication1
Mutagenesisi109L → A: Decreases nuclear expression; when associated with A-104 and A-108. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000308171 – 483ATP-dependent RNA helicase DDX25Add BLAST483

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QY16
PRIDEiQ9QY16

PTM databases

iPTMnetiQ9QY16
PhosphoSitePlusiQ9QY16

Expressioni

Tissue specificityi

Isoform 1 is expressed in germ cells. Isoform 2 is highly expressed in Leydig cells and weakly expressed in the pituitary and hypothalamus. Isoform 3 is weakly expressed only in germ cells.1 Publication

Developmental stagei

Expressed in pubertal and adult animals but not in immature animals.1 Publication

Inductioni

By gonadotropin in Leydig cells. Inhibited by flutamine.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017307

Structurei

3D structure databases

ProteinModelPortaliQ9QY16
SMRiQ9QY16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini130 – 300Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini311 – 478Helicase C-terminalPROSITE-ProRule annotationAdd BLAST168

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi61 – 74Nuclear export signalAdd BLAST14
Motifi97 – 125Q motifAdd BLAST29
Motifi100 – 114Nuclear localization signal1 PublicationAdd BLAST15
Motifi247 – 250DEAD box4

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated

Phylogenomic databases

eggNOGiKOG0332 Eukaryota
ENOG410XRGX LUCA
HOGENOMiHOG000268797
HOVERGENiHBG107989
InParanoidiQ9QY16
KOiK18656
PhylomeDBiQ9QY16

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: Q9QY16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASLLWGGDA GAAESERLNS HFSNLVHPRK NLRGIRSTTV PNIDGSLNTE
60 70 80 90 100
EDDDEDDVVD LAANSLLNKL IRQSLVESSH RVEVLQKDPS SPLYSVKTFE
110 120 130 140 150
ELRLKEELLK GIYAMGFNRP SKIQEMALPM MLAHPPQNLI AQSQSGTGKT
160 170 180 190 200
AAFVLAMLNR VNALELFPQC LCLAPTYELA LQTGRVVERM GKFCVDVEVM
210 220 230 240 250
YAIRGNRIPR GTDVTKQIVI GTPGTVLDWC FKRKLIDLTK IRVFVLDEAD
260 270 280 290 300
VMIDTQGFSD QSIRIQRALP SECQMLLFSA TFEDSVWQFA ERIIPDPNVI
310 320 330 340 350
KLRKEELTLN NIRQYYVLCE NRKDKYQALC NIYGGITIGQ AIIFCQTRRN
360 370 380 390 400
AKWLTVEMMQ DGHQVSLLSG ELTVEQRASI IQRFRDGKEK VLITTNVCAR
410 420 430 440 450
GIDVKQVTIV VNFDLPVNQS EEPDYETYLH RIGRTGRFGK KGLAFNMIEV
460 470 480
DKLPLLMKIQ DHFNSSIKQL DPEDMDEIEK IEY
Length:483
Mass (Da):54,791
Last modified:October 10, 2003 - v2
Checksum:i17F596DAD9E529FC
GO
Isoform 2 (identifier: Q9QY16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Show »
Length:369
Mass (Da):42,134
Checksum:iB9E4E3F63BC2B4E8
GO
Isoform 3 (identifier: Q9QY16-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-189: Missing.

Note: May start at Met-200 rather than Met-190.
Show »
Length:294
Mass (Da):33,923
Checksum:iBC141CC66E7D0613
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q68G14Q68G14_RAT
ATP-dependent RNA helicase DDX25
Ddx25 rCG_22714
483Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188781 – 189Missing in isoform 3. CuratedAdd BLAST189
Alternative sequenceiVSP_0188771 – 114Missing in isoform 2. CuratedAdd BLAST114

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142629 mRNA Translation: AAF21360.2
RefSeqiNP_113818.2, NM_031630.2 [Q9QY16-1]
UniGeneiRn.224821

Genome annotation databases

GeneIDi58856
KEGGirno:58856
UCSCiRGD:68381 rat [Q9QY16-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142629 mRNA Translation: AAF21360.2
RefSeqiNP_113818.2, NM_031630.2 [Q9QY16-1]
UniGeneiRn.224821

3D structure databases

ProteinModelPortaliQ9QY16
SMRiQ9QY16
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017307

PTM databases

iPTMnetiQ9QY16
PhosphoSitePlusiQ9QY16

Proteomic databases

PaxDbiQ9QY16
PRIDEiQ9QY16

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi58856
KEGGirno:58856
UCSCiRGD:68381 rat [Q9QY16-1]

Organism-specific databases

CTDi29118
RGDi68381 Ddx25

Phylogenomic databases

eggNOGiKOG0332 Eukaryota
ENOG410XRGX LUCA
HOGENOMiHOG000268797
HOVERGENiHBG107989
InParanoidiQ9QY16
KOiK18656
PhylomeDBiQ9QY16

Enzyme and pathway databases

BRENDAi3.6.4.12 5301
3.6.4.13 5301

Miscellaneous databases

PROiPR:Q9QY16

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDDX25_RAT
AccessioniPrimary (citable) accession number: Q9QY16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: October 10, 2003
Last modified: October 25, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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