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Protein

EH domain-containing protein 3

Gene

Ehd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes (By similarity). Binding to phosphatidic acid induces its membrane tubulation activity (PubMed:26896729). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology (By similarity). Involved in the recycling of internalized D1 dopamine receptor (By similarity). Plays a role in cardiac protein trafficking probably implicating ANK2. Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte excitability and cardiac conduction (PubMed:20489164, PubMed:24759929, PubMed:25825486). In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function (PubMed:21408024). Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance (By similarity). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing. Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with Ehd1 (PubMed:25686250).By similarity7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPBy similarity1
Binding sitei258ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi65 – 72ATPBy similarity8
Calcium bindingi489 – 500PROSITE-ProRule annotationBy similarityAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • cilium assembly Source: UniProtKB
  • early endosome to Golgi transport Source: MGI
  • endocytic recycling Source: UniProtKB
  • Golgi to lysosome transport Source: MGI
  • positive regulation of voltage-gated calcium channel activity Source: UniProtKB
  • protein homooligomerization Source: UniProtKB
  • protein localization to plasma membrane Source: MGI
  • protein transport Source: UniProtKB-KW
  • receptor recycling Source: MGI
  • regulation of cardiac conduction Source: UniProtKB
  • regulation of cardiac muscle cell membrane potential Source: MGI
  • regulation of cardiac muscle contraction Source: UniProtKB
  • regulation of Golgi organization Source: MGI

Keywordsi

Biological processCilium biogenesis/degradation, Protein transport, Transport
LigandATP-binding, Calcium, Lipid-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 3Curated
Gene namesi
Name:Ehd3Imported
Synonyms:Ehd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1928900 Ehd3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi327K → D: Loss of localization to membranes, reduced binding to phosphatidic acid. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001461131 – 535EH domain-containing protein 3Add BLAST535

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Cross-linki315Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei349PhosphoserineBy similarity1
Modified residuei456PhosphoserineCombined sources1
Cross-linki511Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9QXY6
MaxQBiQ9QXY6
PaxDbiQ9QXY6
PeptideAtlasiQ9QXY6
PRIDEiQ9QXY6

PTM databases

iPTMnetiQ9QXY6
PhosphoSitePlusiQ9QXY6

Expressioni

Tissue specificityi

Strong expression seen in the kidney, brain and liver. In the kidney, expressed exclusively by glomerular endothelial cells; at protein level. Expressed in skeletal muscle neuromuscular junction perisynaptic region; at protein level.3 Publications

Gene expression databases

BgeeiENSMUSG00000024065
CleanExiMM_EHD2
MM_EHD3
GenevisibleiQ9QXY6 MM

Interactioni

Subunit structurei

Homooligomer. Heterooligomer with EHD1 (PubMed:12121420). Heterooligomer with EHD2 and EHD4; ATP-binding is required for heterooligomerization (By similarity). Interacts with PACSIN1 (PubMed:15930129). Interacts with PACSIN2 (PubMed:15930129). Interacts (via EH domain) with MICALL1 (By similarity). Interacts (via EH domain) with RAB11FIP2 (By similarity). Interacts with ANK2 (By similarity). Interacts with CACNA1GG and CACNA1H (PubMed:25825486).By similarity3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi208297, 3 interactors
IntActiQ9QXY6, 13 interactors
MINTiQ9QXY6
STRINGi10090.ENSMUSP00000024860

Structurei

3D structure databases

ProteinModelPortaliQ9QXY6
SMRiQ9QXY6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 286Dynamin-type GPROSITE-ProRule annotationAdd BLAST232
Domaini444 – 532EHPROSITE-ProRule annotationAdd BLAST89
Domaini476 – 511EF-handPROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 72G1 motifPROSITE-ProRule annotation8
Regioni91 – 92G2 motifPROSITE-ProRule annotation2
Regioni153 – 156G3 motifPROSITE-ProRule annotation4
Regioni219 – 222G4 motifPROSITE-ProRule annotation4
Regioni243G5 motifPROSITE-ProRule annotation1

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili198 – 227Sequence analysisAdd BLAST30

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.By similarity

Sequence similaritiesi

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. EHD subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1954 Eukaryota
ENOG410XYGB LUCA
GeneTreeiENSGT00760000118985
HOGENOMiHOG000242040
HOVERGENiHBG018183
InParanoidiQ9QXY6
KOiK12476
OMAiTQRPVQM
OrthoDBiEOG091G04KA
PhylomeDBiQ9QXY6
TreeFamiTF314429

Family and domain databases

CDDicd00052 EH, 1 hit
InterProiView protein in InterPro
IPR022812 Dynamin_SF
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000261 EH_dom
IPR031692 EHD_N
IPR030381 G_DYNAMIN_dom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00350 Dynamin_N, 1 hit
PF12763 EF-hand_4, 1 hit
PF16880 EHD_N, 1 hit
SMARTiView protein in SMART
SM00027 EH, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 1 hit
PS50031 EH, 1 hit
PS51718 G_DYNAMIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QXY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSWLGNDDR RKKDPEVFQT VSDGLKKLYK TKLLPLEEYY RFHEFHSPAL
60 70 80 90 100
EDADFDNKPM VLLVGQYSTG KTTFIRYLLE QDFPGMRIGP EPTTDSFIAV
110 120 130 140 150
MQGDVEGIIP GNALVVDPKK PFRKLNAFGN AFLNRFVCAQ LPNAVLESIS
160 170 180 190 200
VIDTPGILSG EKQRISRGYD FAAVLEWFAE RVDRIILLFD AHKLDISDEF
210 220 230 240 250
SEVIKALKNH EDKMRVVLNK ADQIETQQLM RVYGALMWSL GKIVNTPEVI
260 270 280 290 300
RVYIGSFWSH PLLIPDNRKL FEAEEQDLFR DIQSLPRNAA LRKLNDLIKR
310 320 330 340 350
ARLAKVHAYI ISSLKKEMPS VFGKDTKKKE LVNNLAEIYG RIEREHQISP
360 370 380 390 400
GDFPNLKRMQ DQLQAQDFSK FQPLKSKLLE VVDDMLAHDI AQLMVLVRQE
410 420 430 440 450
ETQRPVQMVK GGAFEGTLQG PFGHGYGEGA GEGIDDAEWV VARDKPMYDE
460 470 480 490 500
IFYTLSPVDG KITGANAKKE MVRSKLPNSV LGKIWKLADI DKDGMLDDEE
510 520 530
FALANHLIKV KLEGHELPSE LPAHLLPPSK RKVSE
Length:535
Mass (Da):60,821
Last modified:March 18, 2008 - v2
Checksum:i80BF1F3B45CB87DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti358R → K in AAF19020 (PubMed:12121420).Curated1
Sequence conflicti529S → Y in AAF19020 (PubMed:12121420).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF155883 mRNA Translation: AAF19020.1
AF506002 mRNA Translation: AAM28633.1
AK141265 mRNA Translation: BAE24623.1
BC046596 mRNA Translation: AAH46596.1
CCDSiCCDS28966.1
RefSeqiNP_065603.2, NM_020578.3
UniGeneiMm.18526

Genome annotation databases

EnsembliENSMUST00000024860; ENSMUSP00000024860; ENSMUSG00000024065
GeneIDi57440
KEGGimmu:57440
UCSCiuc008dnn.2 mouse

Similar proteinsi

Entry informationi

Entry nameiEHD3_MOUSE
AccessioniPrimary (citable) accession number: Q9QXY6
Secondary accession number(s): Q8K590
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 18, 2008
Last modified: July 18, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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