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Protein

Calcium-binding mitochondrial carrier protein Aralar2

Gene

Slc25a13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial and calcium-binding carrier that catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.By similarity

Miscellaneous

Binds to one calcium ion with high affinity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi66CalciumBy similarity1
Metal bindingi70CalciumBy similarity1
Metal bindingi72Calcium; via carbonyl oxygenBy similarity1
Metal bindingi77CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi66 – 771By similarityAdd BLAST12
Calcium bindingi100 – 1112Add BLAST12
Calcium bindingi171 – 1823Add BLAST12

GO - Molecular functioni

GO - Biological processi

  • aspartate transmembrane transport Source: MGI
  • ATP biosynthetic process Source: MGI
  • cellular respiration Source: MGI
  • L-glutamate transmembrane transport Source: UniProtKB
  • malate-aspartate shuttle Source: MGI
  • response to calcium ion Source: UniProtKB

Keywordsi

Biological processTransport
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding mitochondrial carrier protein Aralar2
Alternative name(s):
Citrin
Mitochondrial aspartate glutamate carrier 2
Solute carrier family 25 member 13
Gene namesi
Name:Slc25a13
Synonyms:Aralar2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1354721 Slc25a13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei333 – 350Helical; Name=1By similarityAdd BLAST18
Transmembranei394 – 413Helical; Name=2By similarityAdd BLAST20
Transmembranei437 – 450Helical; Name=3By similarityAdd BLAST14
Transmembranei486 – 505Helical; Name=4By similarityAdd BLAST20
Transmembranei525 – 542Helical; Name=5By similarityAdd BLAST18
Transmembranei582 – 601Helical; Name=6By similarityAdd BLAST20

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000906022 – 676Calcium-binding mitochondrial carrier protein Aralar2Add BLAST675

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei18N6-acetyllysineCombined sources1
Modified residuei354N6-acetyllysineCombined sources1
Modified residuei373N6-acetyllysineCombined sources1
Modified residuei454N6-methyllysineBy similarity1
Modified residuei485N6-acetyllysine; alternateCombined sources1
Modified residuei485N6-succinyllysine; alternateCombined sources1
Modified residuei581N6-succinyllysineCombined sources1
Modified residuei663N6-acetyllysineCombined sources1
Modified residuei667PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9QXX4
MaxQBiQ9QXX4
PaxDbiQ9QXX4
PRIDEiQ9QXX4

PTM databases

iPTMnetiQ9QXX4
PhosphoSitePlusiQ9QXX4
SwissPalmiQ9QXX4

Expressioni

Tissue specificityi

At E10.5, expressed in branchial arches, a well as in the limb and tail buds. At E13.5 expression is predominant in epithelial structures and the forebrain, kidney and liver. Expression in liver is maintained into adulthood.1 Publication

Gene expression databases

BgeeiENSMUSG00000015112
GenevisibleiQ9QXX4 MM

Interactioni

Subunit structurei

Homodimer (via N-terminus).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206130, 6 interactors
IntActiQ9QXX4, 6 interactors
MINTiQ9QXX4
STRINGi10090.ENSMUSP00000015256

Structurei

3D structure databases

ProteinModelPortaliQ9QXX4
SMRiQ9QXX4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 86EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini87 – 122EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini123 – 157EF-hand 3PROSITE-ProRule annotationAdd BLAST35
Domaini158 – 193EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Repeati327 – 419Solcar 1PROSITE-ProRule annotationAdd BLAST93
Repeati427 – 511Solcar 2PROSITE-ProRule annotationAdd BLAST85
Repeati519 – 607Solcar 3PROSITE-ProRule annotationAdd BLAST89

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 295N-terminal domainBy similarityAdd BLAST294
Regioni296 – 311Linker loop domainBy similarityAdd BLAST16
Regioni322 – 613Carrier domainBy similarityAdd BLAST292
Regioni614 – 676C-terminal domainBy similarityAdd BLAST63

Domaini

Upon calcium binding, the EF-hand-containing regulatory N-terminal domain binds to the C-terminal domain, opening a vestibule which allows the substrates to be translocated through the carrier domain (By similarity). In the absence of calcium, the helix loop domain may close the vestibule, which may prevent substrates from entering the carrier domain (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0751 Eukaryota
ENOG410XNRM LUCA
GeneTreeiENSGT00530000062944
HOGENOMiHOG000180633
HOVERGENiHBG005350
InParanoidiQ9QXX4
KOiK15105
OMAiKNGEFFM
OrthoDBiEOG091G08GT
PhylomeDBiQ9QXX4
TreeFamiTF313209

Family and domain databases

Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR002048 EF_hand_dom
IPR002067 Mit_carrier
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF13833 EF-hand_8, 1 hit
PF00153 Mito_carr, 3 hits
PRINTSiPR00926 MITOCARRIER
SUPFAMiSSF103506 SSF103506, 1 hit
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS50222 EF_HAND_2, 3 hits
PS50920 SOLCAR, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QXX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAKVALTK RADPAELKAI FLKYASIEKN GEFFMSPHDF VTRYLNIFGE
60 70 80 90 100
SQPNPKTVEL LSGVVDQTKD GLISFQEFVA FESVLCAPDA LFMVAFQLFD
110 120 130 140 150
KAGKGEVTFE DVKQIFGQTT IHQHIPFNWD SEFVQLHFGK ERKRHLTYAE
160 170 180 190 200
FTQFLLEIQL EHAKQAFVQR DNAKTGKVSA IDFRDIMVTI RPHVLTPFVE
210 220 230 240 250
ECLVAAAGGT RSHQVSFSYF NGFNSLLNNM ELIRKIYSTL AGNRKDVEVT
260 270 280 290 300
KEEFALAAQK FGQVTPMEVD ILFQLADLYE PRGRMTLADI ERIAPLEEGM
310 320 330 340 350
LPFNLAEAQR QQKASGDAAR PFLLQLAESA YRFGLGSIAG AVGATAVYPI
360 370 380 390 400
DLVKTRMQNQ RSTGSFVGEL MYKNSFDCFK KVLRYEGFFG LYRGLLPQLL
410 420 430 440 450
GVAPEKAIKL TVNDFVRDKF MHKDGSVPLL AEIFAGGCAG GSQVIFTNPL
460 470 480 490 500
EIVKIRLQVA GEITTGPRVS ALSVVRDLGF FGIYKGAKAC FLRDIPFSAI
510 520 530 540 550
YFPCYAHVKA SFANEDGQVS PGSLLLAGAI AGMPAASLVT PADVIKTRLQ
560 570 580 590 600
VAARAGQTTY NGVTDCFRKI LREEGPKALW KGVAARVFRS SPQFGVTLLT
610 620 630 640 650
YELLQRWFYV DFGGVKPVGS EPVPKSRITL PAPNPDHVGG YKLAVATFAG
660 670
IENKFGLYLP LFKPSASTSK VTAGDS
Length:676
Mass (Da):74,467
Last modified:May 1, 2000 - v1
Checksum:i5E3952F08E5E58C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312Missing in BAB28397 (PubMed:16141072).Curated1
Sequence conflicti442S → F in BAB22390 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF164632 mRNA Translation: AAF21426.1
AK002829 mRNA Translation: BAB22390.1
AK012670 mRNA Translation: BAB28397.1
CCDSiCCDS39421.1
RefSeqiNP_001171043.1, NM_001177572.1
NP_056644.1, NM_015829.3
UniGeneiMm.24513

Genome annotation databases

EnsembliENSMUST00000015256; ENSMUSP00000015256; ENSMUSG00000015112
ENSMUST00000188414; ENSMUSP00000139571; ENSMUSG00000015112
GeneIDi50799
KEGGimmu:50799
UCSCiuc009awp.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCMC2_MOUSE
AccessioniPrimary (citable) accession number: Q9QXX4
Secondary accession number(s): Q9CZF6, Q9DCF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: June 20, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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