UniProtKB - Q9QXV8 (SPY2_MOUSE)
Protein sprouty homolog 2
Spry2
Functioni
GO - Molecular functioni
- protein kinase binding Source: MGI
- protein serine/threonine kinase activator activity Source: MGI
GO - Biological processi
- animal organ development Source: GO_Central
- branching morphogenesis of an epithelial tube Source: MGI
- bud elongation involved in lung branching Source: MGI
- cell fate commitment Source: MGI
- cellular response to leukemia inhibitory factor Source: MGI
- cellular response to vascular endothelial growth factor stimulus Source: MGI
- establishment of mitotic spindle orientation Source: MGI
- inner ear morphogenesis Source: MGI
- lung development Source: MGI
- lung growth Source: MGI
- lung morphogenesis Source: MGI
- negative regulation of angiogenesis Source: MGI
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of cell population proliferation Source: MGI
- negative regulation of cell projection organization Source: BHF-UCL
- negative regulation of epithelial to mesenchymal transition Source: UniProtKB
- negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
- negative regulation of fibroblast growth factor receptor signaling pathway Source: MGI
- negative regulation of GTPase activity Source: MGI
- negative regulation of lens fiber cell differentiation Source: UniProtKB
- negative regulation of MAP kinase activity Source: MGI
- negative regulation of neurotrophin TRK receptor signaling pathway Source: MGI
- negative regulation of peptidyl-threonine phosphorylation Source: MGI
- negative regulation of protein ubiquitination Source: UniProtKB
- negative regulation of Ras protein signal transduction Source: MGI
- negative regulation of transforming growth factor beta receptor signaling pathway Source: UniProtKB
- negative regulation of vascular endothelial growth factor signaling pathway Source: MGI
- positive regulation of cell migration Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of peptidyl-serine phosphorylation Source: MGI
- positive regulation of protein kinase B signaling Source: MGI
- respiratory system development Source: MGI
- sensory perception of sound Source: MGI
Keywordsi
Molecular function | Developmental protein |
Enzyme and pathway databases
Reactomei | R-MMU-1295596, Spry regulation of FGF signaling R-MMU-182971, EGFR downregulation |
Names & Taxonomyi
Protein namesi | Recommended name: Protein sprouty homolog 2Short name: Spry-2 |
Gene namesi | Name:Spry2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1345138, Spry2 |
Subcellular locationi
Plasma membrane
- ruffle membrane By similarity
Cytoskeleton
- cytoskeleton By similarity
Note: Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).By similarity
Cytoskeleton
- actin cytoskeleton Source: MGI
- cytoskeleton Source: BHF-UCL
- microtubule cytoskeleton Source: MGI
- microtubule end Source: MGI
Cytosol
- cytosol Source: BHF-UCL
Nucleus
- nucleus Source: BHF-UCL
Plasma Membrane
- ruffle membrane Source: UniProtKB-SubCell
Other locations
- membrane Source: BHF-UCL
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, MicrotubulePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 55 | Y → A: Abolishes FGF2-induced lens fiber differentiation via inhibition of FGF-mediated ERK1/2 phosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076902 | 1 – 315 | Protein sprouty homolog 2Add BLAST | 315 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 143 – 144 | Cleavage; by FAPBy similarity | 2 |
Proteomic databases
MaxQBi | Q9QXV8 |
PaxDbi | Q9QXV8 |
PRIDEi | Q9QXV8 |
PTM databases
iPTMneti | Q9QXV8 |
PhosphoSitePlusi | Q9QXV8 |
SwissPalmi | Q9QXV8 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000022114, Expressed in cardiac ventricle and 344 other tissues |
ExpressionAtlasi | Q9QXV8, baseline and differential |
Genevisiblei | Q9QXV8, MM |
Interactioni
Subunit structurei
Forms heterodimers with SPRY1 (PubMed:16877379).
Part of a tripartite complex containing GAB1, METTL13 and SPRY2 (By similarity).
Interacts with METTL13 (By similarity).
Interacts with RAF1 (By similarity).
Interacts (via C-terminus) with TESK1 (via C-terminus); the interaction disrupts SPRY2 interaction with GRB2, potentially via disruption of SPRY2 serine dephosphorylation (PubMed:17974561).
Interacts with PPP2R1A/PP2A-A and PPP2CA/PP2A-C; the interaction with PPP2CA/PP2A-C is inhibited by interaction with TESK1, possibly by vesicular sequestration of SPRY2 (By similarity). Inhibition of the interaction with the serine/threonine-protein phosphatase 2A (PP2A) holoenzyme results in loss of PP2A-mediated dephosphorylation, resulting in the loss of SPRY2 interaction with GRB2 (By similarity).
Interacts with GRB2 (By similarity).
Interacts with CBL/C-CBL; the interaction inhibits CBL-mediated ubiquitination of EGFR (By similarity).
Interacts (via C-terminus) with CAV1 (via C-terminus) (PubMed:16877379).
By similarity2 PublicationsGO - Molecular functioni
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204877, 14 interactors |
IntActi | Q9QXV8, 1 interactor |
STRINGi | 10090.ENSMUSP00000022709 |
Miscellaneous databases
RNActi | Q9QXV8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 177 – 291 | SPRPROSITE-ProRule annotationAdd BLAST | 115 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 117 – 315 | Required for interaction with CAV11 PublicationAdd BLAST | 199 | |
Regioni | 178 – 315 | Required for interaction with TESK1By similarityAdd BLAST | 138 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 124 – 130 | Poly-Ser | 7 | |
Compositional biasi | 178 – 301 | Cys-richAdd BLAST | 124 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG502QTG8, Eukaryota |
GeneTreei | ENSGT00950000183055 |
HOGENOMi | CLU_077696_0_0_1 |
InParanoidi | Q9QXV8 |
OMAi | VDYGTCV |
OrthoDBi | 1157681at2759 |
PhylomeDBi | Q9QXV8 |
TreeFami | TF325070 |
Family and domain databases
InterProi | View protein in InterPro IPR007875, Sprouty IPR030780, SPRY2 |
PANTHERi | PTHR12365:SF8, PTHR12365:SF8, 1 hit |
Pfami | View protein in Pfam PF05210, Sprouty, 1 hit |
PROSITEi | View protein in PROSITE PS51227, SPR, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEARAQSGNG SQPLLQTAHD SGRQRGEPDP RDALTQQVHV LSLDQIRAIR
60 70 80 90 100
NTNEYTEGPT VVPRPGLKPA PRPSTQHKHE RLHGLPEHRQ PPRLQPSQVH
110 120 130 140 150
SSRAPLSRSI STVSSGSRSS TRTSTSSSSS EQRLLGPSFS HGPAAADGII
160 170 180 190 200
RVQPKSELKP GDVKPLSKDD LGLHAYRCED CGKCKCKECT YPRPLPSDWI
210 220 230 240 250
CDKQCLCSAQ NVIDYGTCVC CVKGLFYHCS NDDEDNCADN PCSCSQSHCC
260 270 280 290 300
TRWSAMGVMS LFLPCLWCYL PAKGCLKLCQ GCYDRVNRPG CRCKNSNTVC
310
CKVPTVPPRN FEKPT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 163 | V → I in AAD34167 (PubMed:10074434).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF176905 mRNA Translation: AAD56006.1 AF153084 mRNA Translation: AAD34167.1 |
CCDSi | CCDS27321.1 |
RefSeqi | NP_036027.1, NM_011897.3 XP_006519079.1, XM_006519016.3 |
Genome annotation databases
Ensembli | ENSMUST00000022709; ENSMUSP00000022709; ENSMUSG00000022114 |
GeneIDi | 24064 |
KEGGi | mmu:24064 |
UCSCi | uc007uxy.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF176905 mRNA Translation: AAD56006.1 AF153084 mRNA Translation: AAD34167.1 |
CCDSi | CCDS27321.1 |
RefSeqi | NP_036027.1, NM_011897.3 XP_006519079.1, XM_006519016.3 |
3D structure databases
SMRi | Q9QXV8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204877, 14 interactors |
IntActi | Q9QXV8, 1 interactor |
STRINGi | 10090.ENSMUSP00000022709 |
PTM databases
iPTMneti | Q9QXV8 |
PhosphoSitePlusi | Q9QXV8 |
SwissPalmi | Q9QXV8 |
Proteomic databases
MaxQBi | Q9QXV8 |
PaxDbi | Q9QXV8 |
PRIDEi | Q9QXV8 |
Protocols and materials databases
Antibodypediai | 10392, 294 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000022709; ENSMUSP00000022709; ENSMUSG00000022114 |
GeneIDi | 24064 |
KEGGi | mmu:24064 |
UCSCi | uc007uxy.2, mouse |
Organism-specific databases
CTDi | 10253 |
MGIi | MGI:1345138, Spry2 |
Phylogenomic databases
eggNOGi | ENOG502QTG8, Eukaryota |
GeneTreei | ENSGT00950000183055 |
HOGENOMi | CLU_077696_0_0_1 |
InParanoidi | Q9QXV8 |
OMAi | VDYGTCV |
OrthoDBi | 1157681at2759 |
PhylomeDBi | Q9QXV8 |
TreeFami | TF325070 |
Enzyme and pathway databases
Reactomei | R-MMU-1295596, Spry regulation of FGF signaling R-MMU-182971, EGFR downregulation |
Miscellaneous databases
BioGRID-ORCSi | 24064, 0 hits in 17 CRISPR screens |
ChiTaRSi | Spry2, mouse |
PROi | PR:Q9QXV8 |
RNActi | Q9QXV8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022114, Expressed in cardiac ventricle and 344 other tissues |
ExpressionAtlasi | Q9QXV8, baseline and differential |
Genevisiblei | Q9QXV8, MM |
Family and domain databases
InterProi | View protein in InterPro IPR007875, Sprouty IPR030780, SPRY2 |
PANTHERi | PTHR12365:SF8, PTHR12365:SF8, 1 hit |
Pfami | View protein in Pfam PF05210, Sprouty, 1 hit |
PROSITEi | View protein in PROSITE PS51227, SPR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPY2_MOUSE | |
Accessioni | Q9QXV8Primary (citable) accession number: Q9QXV8 Secondary accession number(s): Q9WUQ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families