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Protein

Lymphoid enhancer-binding factor 1

Gene

Lef1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi297 – 365HMG boxPROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphoid enhancer-binding factor 1
Short name:
LEF-1
Gene namesi
Name:Lef1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620241 Lef1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000485971 – 397Lymphoid enhancer-binding factor 1Add BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki25Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei130PhosphoserineBy similarity1
Modified residuei153Phosphothreonine; by NLKBy similarity1
Modified residuei164Phosphoserine; by NLKBy similarity1
Cross-linki267Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Phosphorylated at Thr-153 and/or Ser-164 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9QXN1
PRIDEiQ9QXN1

PTM databases

iPTMnetiQ9QXN1
PhosphoSitePlusiQ9QXN1

Interactioni

Subunit structurei

Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with TLE1, PIASG, ALYREF/THOC4, EP300, MDFI and MDFIC (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250906, 1 interactor
IntActiQ9QXN1, 1 interactor
MINTiQ9QXN1
STRINGi10116.ENSRNOP00000013694

Structurei

3D structure databases

ProteinModelPortaliQ9QXN1
SMRiQ9QXN1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 60CTNNB1-bindingBy similarityAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 11Poly-Gly6
Compositional biasi12 – 50Asp/Glu-rich (acidic)Add BLAST39
Compositional biasi75 – 271Pro-richAdd BLAST197
Compositional biasi372 – 377Poly-Lys6

Domaini

Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.

Sequence similaritiesi

Belongs to the TCF/LEF family.Curated

Phylogenomic databases

eggNOGiKOG3248 Eukaryota
ENOG41109RU LUCA
HOGENOMiHOG000116032
HOVERGENiHBG000419
InParanoidiQ9QXN1
KOiK04492
PhylomeDBiQ9QXN1

Family and domain databases

Gene3Di1.10.30.10, 1 hit
4.10.900.10, 1 hit
InterProiView protein in InterPro
IPR027397 Catenin_binding_dom_sf
IPR013558 CTNNB1-bd_N
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR028769 LEF1
IPR024940 TCF/LEF
PANTHERiPTHR10373 PTHR10373, 1 hit
PTHR10373:SF11 PTHR10373:SF11, 1 hit
PfamiView protein in Pfam
PF08347 CTNNB1_binding, 1 hit
PF00505 HMG_box, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 1 hit
SUPFAMiSSF47095 SSF47095, 1 hit
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9QXN1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPQLSGGGGG GDPELCATDE MIPFKDEGDP QKEKIFAEIS HPEEEGDLAD
60 70 80 90 100
IKSSLVNESE IIPASNGHEV VGQTQSSQEP YHDKAREHPD DGKHPDGGLY
110 120 130 140 150
NKGPSYSSYS GYIMMPNMNS DPYMSNGSLS PPIPRTSNKV PVVQPSHAVH
160 170 180 190 200
PLTPLITYSD EHFSPGSHPS HIPSEVNPKQ GMSRHPPAPE MPTFYPLSPG
210 220 230 240 250
GVGQITPPLG WQGQPVYPIT GGFRQAYPSS LSGDTSMSRF SHHMIPGPPG
260 270 280 290 300
PHTTGIPHPA IVTPQVKQEH PHTDSDLMHV KPEHEQRKEQ EPKRPHIKKP
310 320 330 340 350
LNAFMLYMKE MRANVVAECT LKESAAINQI LGRRWHALSR EEQAKYYELA
360 370 380 390
RKERQLHMQL YPGWSARDNY GKKKKRKREK LQESTSGTGP RMTAAYI
Length:397
Mass (Da):44,023
Last modified:May 1, 2000 - v1
Checksum:iD6BEFF805CB526EA
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V7C1G3V7C1_RAT
Lymphoid enhancer binding factor 1,...
Lef1 rCG_28787
397Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198533 mRNA Translation: AAF15601.1
RefSeqiNP_569113.1, NM_130429.1
UniGeneiRn.228611

Genome annotation databases

GeneIDi161452
KEGGirno:161452

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198533 mRNA Translation: AAF15601.1
RefSeqiNP_569113.1, NM_130429.1
UniGeneiRn.228611

3D structure databases

ProteinModelPortaliQ9QXN1
SMRiQ9QXN1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250906, 1 interactor
IntActiQ9QXN1, 1 interactor
MINTiQ9QXN1
STRINGi10116.ENSRNOP00000013694

PTM databases

iPTMnetiQ9QXN1
PhosphoSitePlusiQ9QXN1

Proteomic databases

PaxDbiQ9QXN1
PRIDEiQ9QXN1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi161452
KEGGirno:161452

Organism-specific databases

CTDi51176
RGDi620241 Lef1

Phylogenomic databases

eggNOGiKOG3248 Eukaryota
ENOG41109RU LUCA
HOGENOMiHOG000116032
HOVERGENiHBG000419
InParanoidiQ9QXN1
KOiK04492
PhylomeDBiQ9QXN1

Miscellaneous databases

PROiPR:Q9QXN1

Family and domain databases

Gene3Di1.10.30.10, 1 hit
4.10.900.10, 1 hit
InterProiView protein in InterPro
IPR027397 Catenin_binding_dom_sf
IPR013558 CTNNB1-bd_N
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR028769 LEF1
IPR024940 TCF/LEF
PANTHERiPTHR10373 PTHR10373, 1 hit
PTHR10373:SF11 PTHR10373:SF11, 1 hit
PfamiView protein in Pfam
PF08347 CTNNB1_binding, 1 hit
PF00505 HMG_box, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 1 hit
SUPFAMiSSF47095 SSF47095, 1 hit
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLEF1_RAT
AccessioniPrimary (citable) accession number: Q9QXN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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