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Protein

Protein Shroom3

Gene

Shroom3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.4 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Developmental protein
Biological processCell shape

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Shroom3
Gene namesi
Name:Shroom3
Synonyms:Kiaa1481, Shrm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1351655 Shroom3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Death at birth due to defects in neural tube closure causing exencephaly, acrania, facial clefting and spina bifida.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002860671 – 1986Protein Shroom3Add BLAST1986

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei212PhosphoserineCombined sources1
Modified residuei439PhosphoserineCombined sources1
Modified residuei443PhosphoserineCombined sources1
Modified residuei888PhosphoserineBy similarity1
Modified residuei909PhosphothreonineCombined sources1
Modified residuei912PhosphoserineCombined sources1
Modified residuei969PhosphoserineBy similarity1
Modified residuei1063PhosphoserineCombined sources1
Modified residuei1066PhosphoserineCombined sources1
Modified residuei1219PhosphoserineCombined sources1
Isoform 4 (identifier: Q9QXN0-4)
Modified residuei1175PhosphoserineCombined sources1
Modified residuei1179PhosphoserineCombined sources1
Isoform 3 (identifier: Q9QXN0-3)
Modified residuei1350PhosphoserineCombined sources1
Modified residuei1354PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QXN0
PeptideAtlasiQ9QXN0
PRIDEiQ9QXN0

PTM databases

iPTMnetiQ9QXN0
PhosphoSitePlusiQ9QXN0

Expressioni

Developmental stagei

At E8.75, strongly expressed in the cranial neuroepithelium, and also expressed in neural tube, paraxial mesoderm and gut. At E10.5, expressed in neural tube, forebrain, somites, ventral body wall, heart and gut. At E14.5, expression is restricted to skeletal muscle, tips of the digits and forebrain.1 Publication

Gene expression databases

CleanExiMM_SHROOM3

Interactioni

Subunit structurei

Interacts with F-actin.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205229, 5 interactors
IntActiQ9QXN0, 1 interactor
STRINGi10090.ENSMUSP00000108678

Structurei

3D structure databases

ProteinModelPortaliQ9QXN0
SMRiQ9QXN0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 109PDZPROSITE-ProRule annotationAdd BLAST86
Domaini927 – 1023ASD1PROSITE-ProRule annotationAdd BLAST97
Domaini1659 – 1947ASD2PROSITE-ProRule annotationAdd BLAST289

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1844 – 1890Sequence analysisAdd BLAST47

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi191 – 268Ser-richAdd BLAST78
Compositional biasi1351 – 1540Pro-richAdd BLAST190

Domaini

The ASD1 domain mediates F-actin binding.
The ASD2 domain is required for apical constriction induction.

Sequence similaritiesi

Belongs to the shroom family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410II86 Eukaryota
ENOG41113GY LUCA
HOGENOMiHOG000169233
HOVERGENiHBG108489
InParanoidiQ9QXN0
KOiK18625
PhylomeDBiQ9QXN0

Family and domain databases

InterProiView protein in InterPro
IPR014800 ASD1_dom
IPR014799 ASD2_dom
IPR001478 PDZ
IPR036034 PDZ_sf
IPR030494 Shrm3
IPR027685 Shroom_fam
PANTHERiPTHR15012 PTHR15012, 1 hit
PTHR15012:SF33 PTHR15012:SF33, 1 hit
PfamiView protein in Pfam
PF08688 ASD1, 1 hit
PF08687 ASD2, 1 hit
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS51306 ASD1, 1 hit
PS51307 ASD2, 1 hit
PS50106 PDZ, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9QXN0-1) [UniParc]FASTAAdd to basket
Also known as: L,a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKTPENLEEP SATPNPSRTP TERFVYLEAL LEGGAPWGFT LKGGLERGEP
60 70 80 90 100
LIISKIEEGG KADSVSSGLQ AGDEVIHINE VALSSPRREA VSLVKGSYKT
110 120 130 140 150
LRLVVRRDVC AAPGHADPGT SKSLSSELLT CSPQHRKATW SGGVKLRLKQ
160 170 180 190 200
RCSEPATRPH SWHTTKFGET QPDVSMMQIS QGTMGPPWHQ SYHSSSSTSD
210 220 230 240 250
LSNYDHAYLR RSPDQCSSQG SMESLEPSGG YPPCHLLSPA KSTSSIDQLG
260 270 280 290 300
HLHNKRDSAY SSFSTSSSIF EYPPPGGSAR ERSGSMDVIS ARGGLLEGMR
310 320 330 340 350
QADIRYVKTV YDTRRGVSSE YEVNPSALLL QGRDAHASAD SQGCAKWHSI
360 370 380 390 400
PRGKGTPSPS WSQQCSGSLE TATDNLPQKA GAPLPPTRSD SYAAFRHRER
410 420 430 440 450
PSSWSSLDQK RFCRPQTNSS GSQKTPFAED QLHTVPERSP ENSPPVKSKH
460 470 480 490 500
NYTQKAQPGQ PLLPTGIYPV PSPEPHFAQV PQPSVSSNGT VYPALVKESG
510 520 530 540 550
YTAAQGTCNK MATLDENGNQ NEASRPGFAF CQPLEHNSVT PVEKRPEPTA
560 570 580 590 600
KYIYKVHFSS VPENEDSSLK RHITPPHGHS PYPSERKNIH GGSRACSNHH
610 620 630 640 650
SLSSPQAQAL HVGDDRRPSR LSQPWEGDFQ EDHNANLRQK VEREGQGQGL
660 670 680 690 700
SGNSGRTRSA FSSLQNIPES LRRQSNVELG EAQEVHPGGR SKVEDPGRKA
710 720 730 740 750
GASDIRGYLD RSVSYPRPEG KMNAVDSVHS ADSRYEESPA PALPQTSGAS
760 770 780 790 800
QRRLSSSSSA APQYRKPHCS VLEKVSRIEE REQGRHRPLS VGSSAYGPGY
810 820 830 840 850
RPGRTGPTPS TSSSDLDDPK AGSVHFSEST EHLRNGEQNP PNGEAKQEEA
860 870 880 890 900
SRPQCSHLIR RAPADGRGPP ARGGEPSRPE ARLLRSQSTF QLYSEAEREA
910 920 930 940 950
SWSEDRPGTP ESPLLDAPFS RAYRNSIKDA QSRVLGATSF RRRDLEPGTP
960 970 980 990 1000
ATSRPWRPRP ASAHVGMRSP EAAVPSSSPH TPRERHSVTP AAPQAARRGP
1010 1020 1030 1040 1050
RRRLTVEQKK RSYSEPEKMN EVGVSEEAEP TPCGPPRPAQ PRFSESTVAD
1060 1070 1080 1090 1100
RRRIFERDGK ACSTLSLSGP ELKQFQQSAL ADYIQRKTGK RPTGAACTPE
1110 1120 1130 1140 1150
AGLRERAQSA YLQAGPAAPD GPGLASACSL SSLREPEALP RKEHTHPSAA
1160 1170 1180 1190 1200
DGPQAPRDRS SSFASGRLVG ERRRWDPQVP RQLLSGANCE PRGVQRMDGA
1210 1220 1230 1240 1250
PGGPPSWGMV AGKAGKSKSA EDLLERSDTL AVPVHVRSRS SPTSDKKGQD
1260 1270 1280 1290 1300
VLLREGSNFG FVKDPCCLAG PGPRSLSCSD KGQNELALPL HHPTPCWNGS
1310 1320 1330 1340 1350
GCKATVASSA PPESSGAADH LKQRRAPGPR PLSAGMHGHF PDARAASLSS
1360 1370 1380 1390 1400
PLPSPVPSAS PVPSSYRSQL AMDQQTGQQP PSSPASAVTQ PTSPRSPELS
1410 1420 1430 1440 1450
SPAYGLGEGM WKRTSLPQRP PPPWVKWAHA VREDGLAEDT LAPEFANLKH
1460 1470 1480 1490 1500
YRNQPSRPSS CSTSDPDTPG RISLRISESA LQPSPPPRGD YDDEVFMKDL
1510 1520 1530 1540 1550
HPKVTSSPTF EALPPPPPPS PPSEEPLVNG TDDFPPPPPP QALCEVLLDG
1560 1570 1580 1590 1600
EASTEAGSGP CRIPRVMVTR EGHVPGAAHS EGSQIMTATP PQTSAKGSEA
1610 1620 1630 1640 1650
ESNTPSSASA QPQLNGSPGK QLCPSQTRNL TYEPVERTQD LGKKTHAEPQ
1660 1670 1680 1690 1700
KTSEDIRTEA LAKEIVHQDK SLADILDPDS RMKTTMDLME GLFPGDASVL
1710 1720 1730 1740 1750
MDSGAKRKAL DITARRAGCE AKASDHKEAV SVLVNCPAYY SVSAAKAELL
1760 1770 1780 1790 1800
NKIKDMPEEL QEEEGQEDVN EKKAELIGSL THKLESLQEA KGSLLTDIKL
1810 1820 1830 1840 1850
NNALGEEVEA LISELCKPNE FDKYKMFIGD LDKVVNLLLS LSGRLARVEN
1860 1870 1880 1890 1900
VLRGLGEDAS KEERSSLNEK RKVLAGQHED ARELKENLDR RERVVLDILA
1910 1920 1930 1940 1950
NYLSAEQLQD YQHFVKMKST LLIEQRKLDD KIKLGQEQVR CLLESLPSDF
1960 1970 1980
RPKAGAISLP PALTGHATPG GTSVFGGVFP TLTSPL
Length:1,986
Mass (Da):214,888
Last modified:May 1, 2007 - v2
Checksum:i52C7A4AF6390D547
GO
Isoform 2 (identifier: Q9QXN0-2) [UniParc]FASTAAdd to basket
Also known as: S,b

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.

Show »
Length:1,811
Mass (Da):195,981
Checksum:iDE2A33086EE355F8
GO
Isoform 3 (identifier: Q9QXN0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1354-1359: Missing.

Show »
Length:1,980
Mass (Da):214,349
Checksum:i18B675C987AAC60B
GO
Isoform 4 (identifier: Q9QXN0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.
     1354-1359: Missing.

Show »
Length:1,805
Mass (Da):195,442
Checksum:iB8099FC19E506415
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9QMY5E9QMY5_MOUSE
Protein Shroom3
Shroom3
1,980Annotation score:
E9Q6I3E9Q6I3_MOUSE
Protein Shroom3
Shroom3
1,849Annotation score:
A0A286YDQ8A0A286YDQ8_MOUSE
Protein Shroom3
Shroom3
1,899Annotation score:
A0A0R4J1H8A0A0R4J1H8_MOUSE
Protein Shroom3
Shroom3
1,805Annotation score:
G3UXH3G3UXH3_MOUSE
Protein Shroom3
Shroom3
179Annotation score:

Sequence cautioni

The sequence AAF13269 differs from that shown. Reason: Frameshift at positions 1097 and 1124.Curated
The sequence AAF13270 differs from that shown. Reason: Frameshift at positions 1097 and 1124.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti409Q → H in AAF13270 (PubMed:10589677).Curated1
Sequence conflicti409Q → H in AAF13269 (PubMed:10589677).Curated1
Sequence conflicti617R → K in AAF13270 (PubMed:10589677).Curated1
Sequence conflicti617R → K in AAF13269 (PubMed:10589677).Curated1
Sequence conflicti683Q → P in BAC98181 (PubMed:14621295).Curated1
Sequence conflicti800Y → N in AAF13270 (PubMed:10589677).Curated1
Sequence conflicti800Y → N in AAF13269 (PubMed:10589677).Curated1
Sequence conflicti1078S → N in AAF13270 (PubMed:10589677).Curated1
Sequence conflicti1078S → N in AAF13269 (PubMed:10589677).Curated1
Sequence conflicti1239R → K in AAF13270 (PubMed:10589677).Curated1
Sequence conflicti1239R → K in AAF13269 (PubMed:10589677).Curated1
Sequence conflicti1293P → H in AAF13270 (PubMed:10589677).Curated1
Sequence conflicti1293P → H in AAF13269 (PubMed:10589677).Curated1
Sequence conflicti1318A → S in BAC98181 (PubMed:14621295).Curated1
Sequence conflicti1397P → L in AAF13270 (PubMed:10589677).Curated1
Sequence conflicti1397P → L in AAF13269 (PubMed:10589677).Curated1
Sequence conflicti1407 – 1408GE → EK in BAC98181 (PubMed:14621295).Curated2
Sequence conflicti1419R → Q in BAC98181 (PubMed:14621295).Curated1
Sequence conflicti1423P → L in BAC98181 (PubMed:14621295).Curated1
Sequence conflicti1457R → L in BAC98181 (PubMed:14621295).Curated1
Sequence conflicti1770N → Y in AAF13270 (PubMed:10589677).Curated1
Sequence conflicti1770N → Y in AAF13269 (PubMed:10589677).Curated1
Sequence conflicti1967A → G in BAE27948 (PubMed:16141072).Curated1
Sequence conflicti1967A → G in BAE37966 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0249691 – 175Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST175
Alternative sequenceiVSP_0249701354 – 1359Missing in isoform 3 and isoform 4. 2 Publications6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199421 mRNA Translation: AAF13269.1 Frameshift.
AF199422 mRNA Translation: AAF13270.1 Frameshift.
AK147493 mRNA Translation: BAE27948.1
AK164918 mRNA Translation: BAE37966.1
AK129371 mRNA Translation: BAC98181.1
BC003909 mRNA Translation: AAH03909.1
CCDSiCCDS39154.1 [Q9QXN0-3]
CCDS39155.1 [Q9QXN0-4]
RefSeqiNP_001071063.1, NM_001077595.2
NP_001071064.1, NM_001077596.2
NP_056571.2, NM_015756.2
XP_006535031.1, XM_006534968.2
UniGeneiMm.46014

Genome annotation databases

GeneIDi27428
KEGGimmu:27428

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199421 mRNA Translation: AAF13269.1 Frameshift.
AF199422 mRNA Translation: AAF13270.1 Frameshift.
AK147493 mRNA Translation: BAE27948.1
AK164918 mRNA Translation: BAE37966.1
AK129371 mRNA Translation: BAC98181.1
BC003909 mRNA Translation: AAH03909.1
CCDSiCCDS39154.1 [Q9QXN0-3]
CCDS39155.1 [Q9QXN0-4]
RefSeqiNP_001071063.1, NM_001077595.2
NP_001071064.1, NM_001077596.2
NP_056571.2, NM_015756.2
XP_006535031.1, XM_006534968.2
UniGeneiMm.46014

3D structure databases

ProteinModelPortaliQ9QXN0
SMRiQ9QXN0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205229, 5 interactors
IntActiQ9QXN0, 1 interactor
STRINGi10090.ENSMUSP00000108678

PTM databases

iPTMnetiQ9QXN0
PhosphoSitePlusiQ9QXN0

Proteomic databases

PaxDbiQ9QXN0
PeptideAtlasiQ9QXN0
PRIDEiQ9QXN0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi27428
KEGGimmu:27428

Organism-specific databases

CTDi57619
MGIiMGI:1351655 Shroom3
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410II86 Eukaryota
ENOG41113GY LUCA
HOGENOMiHOG000169233
HOVERGENiHBG108489
InParanoidiQ9QXN0
KOiK18625
PhylomeDBiQ9QXN0

Miscellaneous databases

ChiTaRSiShroom3 mouse
PROiPR:Q9QXN0
SOURCEiSearch...

Gene expression databases

CleanExiMM_SHROOM3

Family and domain databases

InterProiView protein in InterPro
IPR014800 ASD1_dom
IPR014799 ASD2_dom
IPR001478 PDZ
IPR036034 PDZ_sf
IPR030494 Shrm3
IPR027685 Shroom_fam
PANTHERiPTHR15012 PTHR15012, 1 hit
PTHR15012:SF33 PTHR15012:SF33, 1 hit
PfamiView protein in Pfam
PF08688 ASD1, 1 hit
PF08687 ASD2, 1 hit
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS51306 ASD1, 1 hit
PS51307 ASD2, 1 hit
PS50106 PDZ, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSHRM3_MOUSE
AccessioniPrimary (citable) accession number: Q9QXN0
Secondary accession number(s): Q3TNX1
, Q6ZPP9, Q99L16, Q9QXM9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: November 7, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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