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Protein

SH2 domain-containing protein 2A

Gene

Sh2d2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could be a T-cell-specific adapter protein involved in the control of T-cell activation. May play a role in p56-LCK-mediated T-cell signaling. Could be involved in the regulation of responses to T-cell activation stimuli, specifically proliferation and lymphokine production. Interactions with ITK and TXK may provide important biochemical links of these two important kinases with other components in the T-cell activation machinery.1 Publication

Miscellaneous

Proliferation of SH2D2A-deficient T-cells in response to T-cell receptor (TCR)-mediated activation is significantly impaired. These activated T-cells are defective in the production of interleukin (IL)-2 and interferon gamma, but not IL-4.

GO - Molecular functioni

  • SH3/SH2 adaptor activity Source: MGI
  • SH3 domain binding Source: UniProtKB-KW

GO - Biological processi

  • cell proliferation Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-4420097 VEGFA-VEGFR2 Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
SH2 domain-containing protein 2A
Alternative name(s):
Lck-associated adapter protein
Short name:
Lad
Rlk/Itk-binding protein
Short name:
Ribp
Gene namesi
Name:Sh2d2a
Synonyms:Lad, Ribp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1351596 Sh2d2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000977271 – 374SH2 domain-containing protein 2AAdd BLAST374

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei237PhosphoserineBy similarity1
Modified residuei316PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues upon TCR-stimulation.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QXK9
PRIDEiQ9QXK9

PTM databases

iPTMnetiQ9QXK9
PhosphoSitePlusiQ9QXK9

Expressioni

Tissue specificityi

Expression limited to tissues of the immune system and, in particular, activated T-cells and natural killer cells. Expressed in the thymus, lymph node, and to a lesser extent, in the spleen and bone marrow. According to PubMed:10553045, also expressed in the lung.

Inductioni

Up-regulated substantially after T-cell activation.

Gene expression databases

BgeeiENSMUSG00000028071 Expressed in 51 organ(s), highest expression level in thymus
CleanExiMM_SH2D2A
ExpressionAtlasiQ9QXK9 baseline and differential
GenevisibleiQ9QXK9 MM

Interactioni

Subunit structurei

Interacts with KDR (By similarity). Interacts with p56-LCK, TXK and ITK.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LckP062403EBI-1644,EBI-1401

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9QXK9, 1 interactor
STRINGi10090.ENSMUSP00000103207

Structurei

3D structure databases

ProteinModelPortaliQ9QXK9
SMRiQ9QXK9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 207SH2PROSITE-ProRule annotationAdd BLAST92

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi267 – 273SH3-bindingSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi267 – 308Pro-richAdd BLAST42

Keywords - Domaini

SH2 domain, SH3-binding

Phylogenomic databases

eggNOGiENOG410IVEH Eukaryota
ENOG4111Y35 LUCA
GeneTreeiENSGT00570000079047
HOGENOMiHOG000008700
HOVERGENiHBG055180
InParanoidiQ9QXK9
KOiK08273
OMAiWFQKTQA
OrthoDBiEOG091G0FTD
PhylomeDBiQ9QXK9
TreeFamiTF336893

Family and domain databases

CDDicd10416 SH2_SH2D2A, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR035884 SH2D2A_SH2
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SUPFAMiSSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9QXK9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEFCLAQPCP QGNHEATSST FNTFQPMNLT QGRCQNLSCG SRPSMQVMKE
60 70 80 90 100
QGVQLSPRTN HTVVSASAPG TAWVLGNADR AEEVPGKGDL SLQAETRAWV
110 120 130 140 150
QKTQAHWLLL KTAPLWFHGF ITRREAERLL QPQPLGCYLV RFSESAVTFV
160 170 180 190 200
LSYRSQTCCR HFLLAQLGDG RHVVLGEDSA HAQLQDLLEH YTECPLSPYG
210 220 230 240 250
EILTQPLARQ TAEPAGLSLR ADSDSGSKRQ DPDTQLSLLL QQGQAQASGH
260 270 280 290 300
TEKVWASQQK ATSQASRPRP PIPAKPQLPP EVYTSPASRL HQAPPINPIY
310 320 330 340 350
QEPDEPIAFY AMGRGSPGDA PSNIYAEVEG PSGTAPIGHP ILRKCWSRPI
360 370
SRGQVREVQG KISSRSRAER GSPS
Length:374
Mass (Da):40,953
Last modified:May 1, 2000 - v1
Checksum:i6215FF96CE764359
GO
Isoform 2 (identifier: Q9QXK9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-261: Missing.

Show »
Length:366
Mass (Da):40,054
Checksum:i536EF3BBF6FDCAAA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14H → R in AAB58422 (PubMed:10553045).Curated1
Sequence conflicti70G → E in AAB58422 (PubMed:10553045).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003967254 – 261Missing in isoform 2. 2 Publications8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69490 mRNA Translation: AAB58422.1
AF203343 mRNA Translation: AAF19396.1
CCDSiCCDS17455.1 [Q9QXK9-2]
CCDS38476.1 [Q9QXK9-1]
RefSeqiNP_067284.1, NM_021309.3 [Q9QXK9-1]
UniGeneiMm.86361

Genome annotation databases

EnsembliENSMUST00000029709; ENSMUSP00000029709; ENSMUSG00000028071 [Q9QXK9-2]
ENSMUST00000107581; ENSMUSP00000103207; ENSMUSG00000028071 [Q9QXK9-1]
GeneIDi27371
KEGGimmu:27371
UCSCiuc008pta.1 mouse [Q9QXK9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69490 mRNA Translation: AAB58422.1
AF203343 mRNA Translation: AAF19396.1
CCDSiCCDS17455.1 [Q9QXK9-2]
CCDS38476.1 [Q9QXK9-1]
RefSeqiNP_067284.1, NM_021309.3 [Q9QXK9-1]
UniGeneiMm.86361

3D structure databases

ProteinModelPortaliQ9QXK9
SMRiQ9QXK9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QXK9, 1 interactor
STRINGi10090.ENSMUSP00000103207

PTM databases

iPTMnetiQ9QXK9
PhosphoSitePlusiQ9QXK9

Proteomic databases

PaxDbiQ9QXK9
PRIDEiQ9QXK9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029709; ENSMUSP00000029709; ENSMUSG00000028071 [Q9QXK9-2]
ENSMUST00000107581; ENSMUSP00000103207; ENSMUSG00000028071 [Q9QXK9-1]
GeneIDi27371
KEGGimmu:27371
UCSCiuc008pta.1 mouse [Q9QXK9-1]

Organism-specific databases

CTDi9047
MGIiMGI:1351596 Sh2d2a

Phylogenomic databases

eggNOGiENOG410IVEH Eukaryota
ENOG4111Y35 LUCA
GeneTreeiENSGT00570000079047
HOGENOMiHOG000008700
HOVERGENiHBG055180
InParanoidiQ9QXK9
KOiK08273
OMAiWFQKTQA
OrthoDBiEOG091G0FTD
PhylomeDBiQ9QXK9
TreeFamiTF336893

Enzyme and pathway databases

ReactomeiR-MMU-4420097 VEGFA-VEGFR2 Pathway

Miscellaneous databases

PROiPR:Q9QXK9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028071 Expressed in 51 organ(s), highest expression level in thymus
CleanExiMM_SH2D2A
ExpressionAtlasiQ9QXK9 baseline and differential
GenevisibleiQ9QXK9 MM

Family and domain databases

CDDicd10416 SH2_SH2D2A, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR035884 SH2D2A_SH2
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PRINTSiPR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SUPFAMiSSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSH22A_MOUSE
AccessioniPrimary (citable) accession number: Q9QXK9
Secondary accession number(s): Q9JHP6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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