Cpsf3

Functionⁱ

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3' endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.3 Publications

Cofactorⁱ

Zn²⁺By similarityNote: Binds 2 Zn²⁺ ions per subunit.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	71	Zinc 1By similarity	1
Metal bindingⁱ	73	Zinc 1By similarity	1
Metal bindingⁱ	75	Zinc 2By similarity	1
Metal bindingⁱ	76	Zinc 2By similarity	1
Metal bindingⁱ	158	Zinc 1By similarity	1
Metal bindingⁱ	179	Zinc 1By similarity	1
Metal bindingⁱ	179	Zinc 2By similarity	1
Active siteⁱ	396	Proton donorSequence analysis	1
Metal bindingⁱ	418	Zinc 2By similarity	1

GO - Molecular functionⁱ

5'-3' exonuclease activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18955505
endoribonuclease activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18955505
metal ion binding Source: UniProtKB-KW
RNA binding
Source: UniProtKB
Inferred from direct assayⁱ
- 18955505

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

mRNA 3'-end processing by stem-loop binding and cleavage
Source: UniProtKB
Inferred from direct assayⁱ
- 18955505

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Endonuclease, Hydrolase, Nuclease, Ribonucleoprotein, RNA-binding
Biological process	mRNA processing
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-MMU-109688 Cleavage of Growing Transcript in the Termination Region R-MMU-72163 mRNA Splicing - Major Pathway R-MMU-72187 mRNA 3'-end processing R-MMU-77595 Processing of Intronless Pre-mRNAs

Reactomeⁱ

R-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72187 mRNA 3'-end processing
R-MMU-77595 Processing of Intronless Pre-mRNAs

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cleavage and polyadenylation specificity factor subunit 3 (EC:3.1.27.-1 Publication) Alternative name(s): Cleavage and polyadenylation specificity factor 73 kDa subunit Short name: CPSF 73 kDa subunit Short name: mRNA 3'-end-processing endonuclease CPSF-73
Gene namesⁱ	Name:Cpsf3 Synonyms:Cpsf73
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 12

Organism-specific databases

MGIⁱ	MGI:1859328 Cpsf3

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000074401	2 – 684	Cleavage and polyadenylation specificity factor subunit 3Add BLAST		683

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylserineBy similarity	1
Cross-linkⁱ	462	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linkⁱ	465	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linkⁱ	545	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residueⁱ	659	PhosphoserineCombined sources	1
Modified residueⁱ	681	PhosphothreonineBy similarity	1

Post-translational modificationⁱ

Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3.By similarity

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q9QXK7
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9QXK7
PaxDbⁱ	Q9QXK7
PeptideAtlas More...PeptideAtlasⁱ	Q9QXK7
PRIDEⁱ	Q9QXK7

PTM databases

iPTMnetⁱ	Q9QXK7
PhosphoSitePlusⁱ	Q9QXK7

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000054309
CleanExⁱ	MM_CPSF3
ExpressionAtlasⁱ	Q9QXK7 baseline and differential
Genevisibleⁱ	Q9QXK7 MM

Interactionⁱ

Subunit structureⁱ

Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with CPSF2, CSTF2 and SYMPK. Interacts with TUT1; the interaction is direct and mediates the recruitment of the CPSF complex on the 3'UTR of pre-mRNAs. Interacts with WDR33 (By similarity). Interacts with ZC3H3 (PubMed:16115198).By similarity1 Publication

Protein-protein interaction databases

BioGridⁱ	207665, 2 interactors
IntActⁱ	Q9QXK7, 1 interactor
Molecular INTeraction database More...MINTⁱ	Q9QXK7
STRINGⁱ	10090.ENSMUSP00000068148

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9QXK7
SMRⁱ	Q9QXK7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG1137 Eukaryota COG1236 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000144162
HOGENOMⁱ	HOG000203394
HOVERGENⁱ	HBG051107
InParanoidⁱ	Q9QXK7
KEGG Orthology (KO) More...KOⁱ	K14403
OMAⁱ	QMLYTEA
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03UN
TreeFamⁱ	TF105643

Family and domain databases

Gene3Dⁱ	3.60.15.10, 2 hits
InterProⁱ	View protein in InterPro IPR022712 Beta_Casp IPR021718 CPSF73-100_C IPR001279 Metallo-B-lactamas IPR036866 RibonucZ/Hydroxyglut_hydro IPR011108 RMMBL
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10996 Beta-Casp, 1 hit PF11718 CPSF73-100_C, 1 hit PF00753 Lactamase_B, 1 hit PF07521 RMMBL, 1 hit
SMARTⁱ	View protein in SMART SM01027 Beta-Casp, 1 hit SM01098 CPSF73-100_C, 1 hit SM00849 Lactamase_B, 1 hit
SUPFAMⁱ	SSF56281 SSF56281, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9QXK7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSAIPAEESD QLLIRPLGAG QEVGRSCIIL EFKGRKIMLD CGIHPGLEGM 
        60         70         80         90        100
DALPYIDLID PAEIDLLLIS HFHLDHCGAL PWFLQKTSFK GRTFMTHATK 
       110        120        130        140        150
AIYRWLLSDY VKVSNISADD MLYTETDLEE SMDKIETINF HEVKEVAGIK 
       160        170        180        190        200
FWCYHAGHVL GAAMFMIEIA GVKLLYTGDF SRQEDRHLMA AEIPNIKPDI 
       210        220        230        240        250
LIIESTYGTH IHEKREEREA RFCNTVHDIV NRGGRGLIPV FALGRAQELL 
       260        270        280        290        300
LILDEYWQNH PELHDIPIYY ASSLAKKCMA VYQTYVNAMN DKIRKQININ 
       310        320        330        340        350
NPFVFKHISN LKSMDHFDDI GPSVVMASPG MIQNGLSREL FESWCTDKRN 
       360        370        380        390        400
GVIIAGYCVE GTLAKHIMSE PEEITTMSGQ KLPLKMSVDY ISFSAHTDYQ 
       410        420        430        440        450
QTSEFIRALK PPHVILVHGE QNEMARLKAA LIREYEDNDE VHIEVHNPRN 
       460        470        480        490        500
TEAVTLNFRG EKLAKVMGFL ADKKPEQGQR VSGILVKRNF NYHILSPCDL 
       510        520        530        540        550
SNYTDLAMST VKQTQAIPYT GPFYLLYYQL QKLTGDVEEL EIQEKPALKV 
       560        570        580        590        600
FKSITVVQEP GMVVLEWLAN PSNDMYADTV TTVILEVQSN PKIRKGAVQK 
       610        620        630        640        650
VSKKLEMHVY SKRLEVMLQD IFGEDCVSVK DDSVLSVTVD GKTANINLET 
       660        670        680 
RAVECEEGSE DDESLREMVE LAAQRLYEAL TPVH

Length:684

Mass (Da):77,505

Last modified:July 27, 2011 - v2

Checksum:ⁱ043D09F54284B423

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	223 – 224	CN → WH in AAF19420 (Ref. 1) Curated	2
Sequence conflictⁱ	541	E → D in AAF19420 (Ref. 1) Curated	1
Sequence conflictⁱ	564 – 565	VL → GS in AAF19420 (Ref. 1) Curated	2

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF203969 mRNA Translation: AAF19420.1 BC023297 mRNA Translation: AAH23297.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS25834.1
NCBI Reference Sequences More...RefSeqⁱ	NP_061283.2, NM_018813.3
UniGeneⁱ	Mm.356778

Genome annotation databases

Ensemblⁱ	ENSMUST00000067284; ENSMUSP00000068148; ENSMUSG00000054309
GeneIDⁱ	54451
KEGGⁱ	mmu:54451
UCSC genome browser More...UCSCⁱ	uc007ndp.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q9QXK7	UPI0003D76E8D		MOUSE		647
Uncharacterized protein (Fragment)		MOUSE		206
UPI0007EDF2E2		MOUSE		564
Uncharacterized protein		MOUSE		406

Protein	Similar proteins		Species	Score	Length
Q9QXK7	Cleavage and polyadenylation specificity factor subunit 3	)	HUMAN		684
UPI0004C020DB		CALAN		735
UPI000A3CC388		COLLI		724
Cleavage and polyadenylation specific factor 3, 73kDa		ZONAL		721
UPI0004D06C24		GALVR		717
+268

Protein	Similar proteins		Species	Score	Length
Q9QXK7	Cleavage and polyadenylation specificity factor subunit 3	)	HUMAN		684
UPI0004C020DB		CALAN		735
UPI000A3CC388		COLLI		724
Cleavage and polyadenylation specific factor 3, 73kDa		ZONAL		721
UPI0004D06C24		GALVR		717
+498

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF203969 mRNA Translation: AAF19420.1 BC023297 mRNA Translation: AAH23297.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS25834.1
NCBI Reference Sequences More...RefSeqⁱ	NP_061283.2, NM_018813.3
UniGeneⁱ	Mm.356778

3D structure databases

ProteinModelPortalⁱ	Q9QXK7
SMRⁱ	Q9QXK7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	207665, 2 interactors
IntActⁱ	Q9QXK7, 1 interactor
Molecular INTeraction database More...MINTⁱ	Q9QXK7
STRINGⁱ	10090.ENSMUSP00000068148

PTM databases

iPTMnetⁱ	Q9QXK7
PhosphoSitePlusⁱ	Q9QXK7

Proteomic databases

EPDⁱ	Q9QXK7
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9QXK7
PaxDbⁱ	Q9QXK7
PeptideAtlas More...PeptideAtlasⁱ	Q9QXK7
PRIDEⁱ	Q9QXK7

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000067284; ENSMUSP00000068148; ENSMUSG00000054309
GeneIDⁱ	54451
KEGGⁱ	mmu:54451
UCSC genome browser More...UCSCⁱ	uc007ndp.2 mouse

Organism-specific databases

CTDⁱ	51692
MGIⁱ	MGI:1859328 Cpsf3

Phylogenomic databases

eggNOGⁱ	KOG1137 Eukaryota COG1236 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000144162
HOGENOMⁱ	HOG000203394
HOVERGENⁱ	HBG051107
InParanoidⁱ	Q9QXK7
KEGG Orthology (KO) More...KOⁱ	K14403
OMAⁱ	QMLYTEA
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G03UN
TreeFamⁱ	TF105643

Enzyme and pathway databases

Reactomeⁱ	R-MMU-109688 Cleavage of Growing Transcript in the Termination Region R-MMU-72163 mRNA Splicing - Major Pathway R-MMU-72187 mRNA 3'-end processing R-MMU-77595 Processing of Intronless Pre-mRNAs

Reactomeⁱ

R-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72187 mRNA 3'-end processing
R-MMU-77595 Processing of Intronless Pre-mRNAs

Miscellaneous databases

ChiTaRSⁱ	Cpsf3 mouse
Protein Ontology More...PROⁱ	PR:Q9QXK7
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000054309
CleanExⁱ	MM_CPSF3
ExpressionAtlasⁱ	Q9QXK7 baseline and differential
Genevisibleⁱ	Q9QXK7 MM

Family and domain databases

Gene3Dⁱ	3.60.15.10, 2 hits
InterProⁱ	View protein in InterPro IPR022712 Beta_Casp IPR021718 CPSF73-100_C IPR001279 Metallo-B-lactamas IPR036866 RibonucZ/Hydroxyglut_hydro IPR011108 RMMBL
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10996 Beta-Casp, 1 hit PF11718 CPSF73-100_C, 1 hit PF00753 Lactamase_B, 1 hit PF07521 RMMBL, 1 hit
SMARTⁱ	View protein in SMART SM01027 Beta-Casp, 1 hit SM01098 CPSF73-100_C, 1 hit SM00849 Lactamase_B, 1 hit
SUPFAMⁱ	SSF56281 SSF56281, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CPSF3_MOUSE
Accessionⁱ	Q9QXK7Primary (citable) accession number: Q9QXK7 Secondary accession number(s): Q8CIM0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
	Last sequence update:	July 27, 2011
	Last modified:	May 23, 2018
	This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

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Annotation systems

Supporting data

UniProtKB - Q9QXK7 (CPSF3_MOUSE)

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Cleavage and polyadenylation specificity factor subunit 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ