UniProtKB - Q9QXJ1 (APBB1_MOUSE)
Amyloid beta precursor protein binding family B member 1
Apbb1
Functioni
Transcription coregulator that can have both coactivator and corepressor functions (PubMed:17121854, PubMed:25342469).
Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain (PubMed:17121854, PubMed:25342469).
Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs) (By similarity).
Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity (By similarity).
Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s) (By similarity).
Involved in hippocampal neurite branching and neuromuscular junction formation, as a result plays a role in spatial memory functioning (PubMed:27734846).
Plays a role in the maintenance of lens transparency (PubMed:25757569).
May play a role in muscle cell strength (PubMed:25757569, PubMed:27734846).
By similarity4 PublicationsGO - Molecular functioni
- amyloid-beta binding Source: MGI
- chromatin binding Source: MGI
- DNA-binding transcription factor binding Source: MGI
- histone binding Source: MGI
- proline-rich region binding Source: MGI
- protein-containing complex binding Source: MGI
- tau protein binding Source: MGI
- ubiquitin protein ligase binding Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- axon guidance Source: MGI
- cellular response to DNA damage stimulus Source: UniProtKB
- chromatin organization Source: UniProtKB-KW
- double-strand break repair Source: UniProtKB
- extracellular matrix organization Source: MGI
- histone H4 acetylation Source: UniProtKB
- negative regulation of cell cycle G1/S phase transition Source: UniProtKB
- negative regulation of neuron differentiation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- neuron migration Source: MGI
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of DNA repair Source: UniProtKB
- positive regulation of neuron projection development Source: MGI
- positive regulation of protein secretion Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- protein stabilization Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
- smooth muscle contraction Source: UniProtKB
- synapse organization Source: SynGO
Keywordsi
Molecular function | Activator, Chromatin regulator, Repressor |
Biological process | Apoptosis, DNA damage, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
Names & Taxonomyi
Protein namesi | Recommended name: Amyloid beta precursor protein binding family B member 1By similarityAlternative name(s): Amyloid-beta A4 precursor protein-binding family B member 1Imported Protein Fe651 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107765, Apbb1 |
VEuPathDBi | HostDB:ENSMUSG00000037032 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Nucleus
- Nucleus 1 Publication
- Nucleus speckle
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- growth cone 1 Publication
Note: Colocalizes with TSHZ3 in the nucleus and in axonal growth cone (PubMed:19343227). Colocalizes with TSHZ3 in axonal growth cone (By similarity). Colocalizes with TSHZ3 in the nucleus (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP (By similarity). Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus (By similarity). Nuclear translocation is under the regulation of APP (By similarity). Colocalizes with NEK6 at the nuclear speckles (By similarity). Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity).By similarity1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Nucleus
- nuclear speck Source: UniProtKB-SubCell
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
- postsynaptic membrane Source: MGI
- presynaptic membrane Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- dendritic spine Source: MGI
- glutamatergic synapse Source: SynGO
- growth cone Source: UniProtKB
- growth cone filopodium Source: MGI
- growth cone lamellipodium Source: MGI
- lamellipodium Source: UniProtKB
- main axon Source: MGI
- neuromuscular junction Source: SynGO
- neuronal cell body Source: MGI
- perinuclear region of cytoplasm Source: MGI
- presynapse Source: MGI
- protein-containing complex Source: MGI
- Schaffer collateral - CA1 synapse Source: SynGO
- synapse Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 280 | W → F: Abolishes ligand binding; when associated with A-283. 1 Publication | 1 | |
Mutagenesisi | 283 | P → A: Abolishes ligand binding; when associated with F-280. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076050 | 1 – 710 | Amyloid beta precursor protein binding family B member 1Add BLAST | 710 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 135 | PhosphoserineCombined sources | 1 | |
Modified residuei | 204 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 517 | PhosphoserineCombined sources | 1 | |
Modified residuei | 547 | Phosphotyrosine; by ABL1By similarity | 1 | |
Modified residuei | 610 | Phosphoserine; by SGK1By similarity | 1 | |
Modified residuei | 701 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q9QXJ1 |
MaxQBi | Q9QXJ1 |
PaxDbi | Q9QXJ1 |
PRIDEi | Q9QXJ1 |
ProteomicsDBi | 296328 [Q9QXJ1-1] 296329 [Q9QXJ1-2] |
PTM databases
iPTMneti | Q9QXJ1 |
PhosphoSitePlusi | Q9QXJ1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000037032, Expressed in primary motor cortex and 305 other tissues |
Genevisiblei | Q9QXJ1, MM |
Interactioni
Subunit structurei
Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP (By similarity).
Interacts (via PID domain 2) with APP (with the intracellular domain of the amyloid-beta precursor protein) (By similarity).
Interacts (via PID domain 2) with RASD1/DEXRAS1; impairs the transcription activation activity (By similarity).
Interacts (via PID domain 1) with KAT5/TIP60 (By similarity).
Interacts (via the WW domain) with the proline-rich region of APBB1IP (PubMed:9407065).
Interacts with TSHZ1 and TSHZ2 (PubMed:19343227).
Interacts (via the WW domain) with histone H2AX (when phosphorylated on 'Tyr-142') and the proline-rich region of ENAH (PubMed:9407065).
Interacts with MAPK8 (By similarity).
Interacts (via PID domain 1) with TSHZ3 (via homeobox domain) (By similarity).
Interacts with SET (By similarity).
Found in a trimeric complex with HDAC1 and TSHZ3; the interaction between HDAC1 and APBB1 is mediated by TSHZ3 (By similarity).
Interacts (via WWW domain) with NEK6.
Interacts (via WWW domain) with ABL1.
Interacts with RNF157 (By similarity).
By similarity2 PublicationsBinary interactionsi
Q9QXJ1
With | #Exp. | IntAct |
---|---|---|
App [P12023] | 2 | EBI-81338,EBI-78814 |
GO - Molecular functioni
- DNA-binding transcription factor binding Source: MGI
- histone binding Source: MGI
- proline-rich region binding Source: MGI
- tau protein binding Source: MGI
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 198141, 6 interactors |
CORUMi | Q9QXJ1 |
IntActi | Q9QXJ1, 2 interactors |
MINTi | Q9QXJ1 |
STRINGi | 10090.ENSMUSP00000140116 |
Miscellaneous databases
RNActi | Q9QXJ1, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 253 – 285 | WWPROSITE-ProRule annotationAdd BLAST | 33 | |
Domaini | 370 – 509 | PID 1PROSITE-ProRule annotationAdd BLAST | 140 | |
Domaini | 542 – 699 | PID 2PROSITE-ProRule annotationAdd BLAST | 158 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 143 – 256 | DisorderedSequence analysisAdd BLAST | 114 | |
Regioni | 276 – 300 | DisorderedSequence analysisAdd BLAST | 25 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 152 – 175 | Acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 208 – 249 | Polar residuesSequence analysisAdd BLAST | 42 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502QT08, Eukaryota |
GeneTreei | ENSGT00390000000002 |
InParanoidi | Q9QXJ1 |
OrthoDBi | 437627at2759 |
TreeFami | TF314331 |
Family and domain databases
CDDi | cd00201, WW, 1 hit |
Gene3Di | 2.30.29.30, 2 hits |
InterProi | View protein in InterPro IPR039576, Fe65 IPR011993, PH-like_dom_sf IPR006020, PTB/PI_dom IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR14058, PTHR14058, 1 hit |
Pfami | View protein in Pfam PF00640, PID, 2 hits PF00397, WW, 1 hit |
SMARTi | View protein in SMART SM00462, PTB, 2 hits SM00456, WW, 1 hit |
SUPFAMi | SSF51045, SSF51045, 1 hit |
PROSITEi | View protein in PROSITE PS01179, PID, 2 hits PS01159, WW_DOMAIN_1, 1 hit PS50020, WW_DOMAIN_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 16 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSVPSSLSQS AINANSHGGP ALSFPLPLHA AHNQLLNAKL QATAVVPKDL
60 70 80 90 100
RSAMGEGSVP EPGPANAKWL KEGQNQLRRA ATAHRDQNRN VTLTLAEEAS
110 120 130 140 150
QEAETAPLGP KGLMHLYSEL ELSAHNAANR GLHGSALIIN TQEQGPDEGE
160 170 180 190 200
EKAAGEAEED DEDEEEEEEE EDLSSPPGLP EPLENVEVPS GPQALTDGPR
210 220 230 240 250
EHSKSASLLF GMRNSAASDE DSSWATLSQG SPSYGSPEDT DSFWNPNAFE
260 270 280 290 300
TDSDLPAGWM RVQDTSGTYY WHIPTGTTQW EPPGRASPSQ GSSPQEESQL
310 320 330 340 350
TWTGFAHQEG FEEGEFWKDE PSEEAPMELG LKDPEEATLS FPAQSLSPEP
360 370 380 390 400
VPQEEEKLSQ RNANPGIKCF AVRSLGWVEM TEEELAPGRS SVAVNNCIRQ
410 420 430 440 450
LSYHKNNLHD PMAGGWGEGK DLLLQLEDET LKLVEPQNQT LLHAQPIVSI
460 470 480 490 500
RVWGVGRDSG SNRDFAYVAR DKLTQMLKCH VFRCEAPAKN IATSLHEICS
510 520 530 540 550
KIMSERRNAR CLVNGLSLDH SKLVDVPFQV EFPAPKNELV QKFQVYYLGN
560 570 580 590 600
VPVAKPVGVD VINGALESVL SSSSREQWTP SHVSVAPATL TILHQQTEAV
610 620 630 640 650
LGECRVRFLS FLAVGRDVHT FAFIMAAGPA SFCCHMFWCE PNAASLSEAV
660 670 680 690 700
QAACMLRYQK CLDARSQTST SCLPAPPAES VARRVGWTVR RGVQSLWGSL
710
KPKRLGSQTP
Computationally mapped potential isoform sequencesi
There are 16 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J2C1 | A0A0R4J2C1_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 710 | Annotation score: | ||
A0A087WR62 | A0A087WR62_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 451 | Annotation score: | ||
A0A087WRP1 | A0A087WRP1_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 223 | Annotation score: | ||
A0A0R4J2C6 | A0A0R4J2C6_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 708 | Annotation score: | ||
A0A087WNN4 | A0A087WNN4_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 158 | Annotation score: | ||
A0A087WPF4 | A0A087WPF4_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 120 | Annotation score: | ||
A0A1B0GSI3 | A0A1B0GSI3_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 122 | Annotation score: | ||
A0A087WPI2 | A0A087WPI2_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 487 | Annotation score: | ||
A0A087WPS7 | A0A087WPS7_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 485 | Annotation score: | ||
A0A087WQH0 | A0A087WQH0_MOUSE | Amyloid beta precursor protein-bind... | Apbb1 | 260 | Annotation score: | ||
There are more potential isoformsShow all |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 92 | T → A in AAF20141 (Ref. 1) Curated | 1 | |
Sequence conflicti | 313 | E → D in AAF20141 (Ref. 1) Curated | 1 | |
Sequence conflicti | 461 – 462 | SN → RE in BAC39033 (PubMed:16141072).Curated | 2 | |
Sequence conflicti | 461 – 462 | SN → RE in BAE37645 (PubMed:16141072).Curated | 2 | |
Sequence conflicti | 630 | A → S in BAC39033 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011659 | 462 – 463 | Missing in isoform 2. 2 Publications | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF206720 mRNA Translation: AAF20141.1 AK030748 mRNA Translation: BAC27116.1 AK083830 mRNA Translation: BAC39033.1 AK164140 mRNA Translation: BAE37645.1 AC125227 Genomic DNA No translation available. L77865 mRNA Translation: AAB51603.1 |
CCDSi | CCDS80775.1 [Q9QXJ1-2] CCDS80776.1 [Q9QXJ1-1] |
RefSeqi | NP_001240814.1, NM_001253885.1 NP_001240815.1, NM_001253886.1 NP_033815.1, NM_009685.3 XP_006507293.1, XM_006507230.2 XP_006507294.1, XM_006507231.1 XP_017177435.1, XM_017321946.1 |
Genome annotation databases
Ensembli | ENSMUST00000081165; ENSMUSP00000079932; ENSMUSG00000037032 [Q9QXJ1-1] |
GeneIDi | 11785 |
KEGGi | mmu:11785 |
UCSCi | uc009iyi.2, mouse [Q9QXJ1-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF206720 mRNA Translation: AAF20141.1 AK030748 mRNA Translation: BAC27116.1 AK083830 mRNA Translation: BAC39033.1 AK164140 mRNA Translation: BAE37645.1 AC125227 Genomic DNA No translation available. L77865 mRNA Translation: AAB51603.1 |
CCDSi | CCDS80775.1 [Q9QXJ1-2] CCDS80776.1 [Q9QXJ1-1] |
RefSeqi | NP_001240814.1, NM_001253885.1 NP_001240815.1, NM_001253886.1 NP_033815.1, NM_009685.3 XP_006507293.1, XM_006507230.2 XP_006507294.1, XM_006507231.1 XP_017177435.1, XM_017321946.1 |
3D structure databases
BMRBi | Q9QXJ1 |
SMRi | Q9QXJ1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198141, 6 interactors |
CORUMi | Q9QXJ1 |
IntActi | Q9QXJ1, 2 interactors |
MINTi | Q9QXJ1 |
STRINGi | 10090.ENSMUSP00000140116 |
PTM databases
iPTMneti | Q9QXJ1 |
PhosphoSitePlusi | Q9QXJ1 |
Proteomic databases
jPOSTi | Q9QXJ1 |
MaxQBi | Q9QXJ1 |
PaxDbi | Q9QXJ1 |
PRIDEi | Q9QXJ1 |
ProteomicsDBi | 296328 [Q9QXJ1-1] 296329 [Q9QXJ1-2] |
Protocols and materials databases
Antibodypediai | 23808, 401 antibodies from 39 providers |
DNASUi | 11785 |
Genome annotation databases
Ensembli | ENSMUST00000081165; ENSMUSP00000079932; ENSMUSG00000037032 [Q9QXJ1-1] |
GeneIDi | 11785 |
KEGGi | mmu:11785 |
UCSCi | uc009iyi.2, mouse [Q9QXJ1-2] |
Organism-specific databases
CTDi | 322 |
MGIi | MGI:107765, Apbb1 |
VEuPathDBi | HostDB:ENSMUSG00000037032 |
Phylogenomic databases
eggNOGi | ENOG502QT08, Eukaryota |
GeneTreei | ENSGT00390000000002 |
InParanoidi | Q9QXJ1 |
OrthoDBi | 437627at2759 |
TreeFami | TF314331 |
Enzyme and pathway databases
Reactomei | R-MMU-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks |
Miscellaneous databases
BioGRID-ORCSi | 11785, 1 hit in 94 CRISPR screens |
ChiTaRSi | Apbb1, mouse |
PROi | PR:Q9QXJ1 |
RNActi | Q9QXJ1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037032, Expressed in primary motor cortex and 305 other tissues |
Genevisiblei | Q9QXJ1, MM |
Family and domain databases
CDDi | cd00201, WW, 1 hit |
Gene3Di | 2.30.29.30, 2 hits |
InterProi | View protein in InterPro IPR039576, Fe65 IPR011993, PH-like_dom_sf IPR006020, PTB/PI_dom IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR14058, PTHR14058, 1 hit |
Pfami | View protein in Pfam PF00640, PID, 2 hits PF00397, WW, 1 hit |
SMARTi | View protein in SMART SM00462, PTB, 2 hits SM00456, WW, 1 hit |
SUPFAMi | SSF51045, SSF51045, 1 hit |
PROSITEi | View protein in PROSITE PS01179, PID, 2 hits PS01159, WW_DOMAIN_1, 1 hit PS50020, WW_DOMAIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | APBB1_MOUSE | |
Accessioni | Q9QXJ1Primary (citable) accession number: Q9QXJ1 Secondary accession number(s): E9QNW5 Q8BSR9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot