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Protein

Amyloid-beta A4 precursor protein-binding family B member 1

Gene

Apbb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).2 Publications

GO - Molecular functioni

  • amyloid-beta binding Source: UniProtKB
  • chromatin binding Source: MGI
  • histone acetyltransferase binding Source: UniProtKB
  • histone binding Source: MGI
  • proline-rich region binding Source: MGI
  • protein-containing complex binding Source: MGI
  • tau protein binding Source: MGI
  • transcription factor binding Source: UniProtKB

GO - Biological processi

  • actin filament-based movement Source: UniProtKB
  • apoptotic process Source: UniProtKB-KW
  • axon guidance Source: MGI
  • cell cycle arrest Source: UniProtKB
  • cellular response to DNA damage stimulus Source: UniProtKB
  • double-strand break repair Source: UniProtKB
  • extracellular matrix organization Source: MGI
  • histone H4 acetylation Source: UniProtKB
  • negative regulation of cell growth Source: UniProtKB
  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of thymidylate synthase biosynthetic process Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • neuron migration Source: MGI
  • positive regulation of apoptotic process Source: UniProtKB
  • positive regulation of DNA repair Source: UniProtKB
  • positive regulation of neuron projection development Source: MGI
  • positive regulation of protein secretion Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • protein stabilization Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • visual learning Source: MGI

Keywordsi

Molecular functionActivator, Chromatin regulator, Repressor
Biological processApoptosis, DNA damage, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Amyloid-beta A4 precursor protein-binding family B member 1
Alternative name(s):
Protein Fe65
Gene namesi
Name:Apbb1
Synonyms:Fe65
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:107765 Apbb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

No phenotype in normal conditions. Displays an increased sensitivity to genotoxic stress and exposur to DNA damaging agents.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi280W → F: Abolishes ligand binding; when associated with A-283. 1 Publication1
Mutagenesisi283P → A: Abolishes ligand binding; when associated with F-280. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760501 – 710Amyloid-beta A4 precursor protein-binding family B member 1Add BLAST710

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135PhosphoserineCombined sources1
Modified residuei517PhosphoserineCombined sources1
Modified residuei547Phosphotyrosine; by ABL1By similarity1
Modified residuei610Phosphoserine; by SGK1By similarity1

Post-translational modificationi

Polyubiquitination by RNF157 leads to degradation by the proteasome (PubMed:25342469).1 Publication
Phosphorylated following nuclear translocation. Phosphorylation at Tyr-546 enhances the transcription activation activity and reduces the affinity with RASD1/DEXRAS1. Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9QXJ1
PRIDEiQ9QXJ1

PTM databases

iPTMnetiQ9QXJ1
PhosphoSitePlusiQ9QXJ1

Expressioni

Gene expression databases

BgeeiENSMUSG00000037032
ExpressionAtlasiQ9QXJ1 baseline and differential
GenevisibleiQ9QXJ1 MM

Interactioni

Subunit structurei

Interacts with SET. Found in a trimeric complex with HDAC1 and TSHZ3; the interaction between HDAC1 and APBB1 is mediated by TSHZ3 (By similarity). Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP. Interacts (via PID domain 2) with APP (with the intracellular domain of the amyloid-beta precursor protein). Interacts (via PID domain 2) with RASD1/DEXRAS1; impairs the transcription activation activity. Interacts (via PID domain 1) with KAT5/TIP60. Interacts (via the WW domain) with histone H2AX (when phosphorylated on 'Tyr-142'). Interacts with MAPK8 (By similarity). Interacts (via the WW domain) with proline-rich regions of APBB1IP and ENAH. Interacts (via PID domain 1) with TSHZ3 (via homeobox domain). Interacts with TSHZ1 and TSHZ2. Interacts (via WWW domain) with NEK6. Interacts (via WWW domain) with ABL1 (By similarity). Interacts with RNF157 (PubMed:25342469).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AppP120232EBI-81338,EBI-78814

GO - Molecular functioni

  • histone acetyltransferase binding Source: UniProtKB
  • histone binding Source: MGI
  • proline-rich region binding Source: MGI
  • tau protein binding Source: MGI
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi198141, 6 interactors
IntActiQ9QXJ1, 2 interactors
MINTiQ9QXJ1
STRINGi10090.ENSMUSP00000079932

Structurei

3D structure databases

ProteinModelPortaliQ9QXJ1
SMRiQ9QXJ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini253 – 285WWPROSITE-ProRule annotationAdd BLAST33
Domaini370 – 509PID 1PROSITE-ProRule annotationAdd BLAST140
Domaini542 – 699PID 2PROSITE-ProRule annotationAdd BLAST158

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi158 – 171Glu-richAdd BLAST14

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IEKA Eukaryota
ENOG410YEVS LUCA
GeneTreeiENSGT00390000000002
HOGENOMiHOG000033983
HOVERGENiHBG050524
InParanoidiQ9QXJ1
KOiK04529
OrthoDBiEOG091G04HV
TreeFamiTF314331

Family and domain databases

CDDicd00201 WW, 1 hit
Gene3Di2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR006020 PTB/PI_dom
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00640 PID, 2 hits
PF00397 WW, 1 hit
SMARTiView protein in SMART
SM00462 PTB, 2 hits
SM00456 WW, 1 hit
SUPFAMiSSF51045 SSF51045, 1 hit
PROSITEiView protein in PROSITE
PS01179 PID, 2 hits
PS01159 WW_DOMAIN_1, 1 hit
PS50020 WW_DOMAIN_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QXJ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVPSSLSQS AINANSHGGP ALSFPLPLHA AHNQLLNAKL QATAVVPKDL
60 70 80 90 100
RSAMGEGSVP EPGPANAKWL KEGQNQLRRA ATAHRDQNRN VTLTLAEEAS
110 120 130 140 150
QEAETAPLGP KGLMHLYSEL ELSAHNAANR GLHGSALIIN TQEQGPDEGE
160 170 180 190 200
EKAAGEAEED DEDEEEEEEE EDLSSPPGLP EPLENVEVPS GPQALTDGPR
210 220 230 240 250
EHSKSASLLF GMRNSAASDE DSSWATLSQG SPSYGSPEDT DSFWNPNAFE
260 270 280 290 300
TDSDLPAGWM RVQDTSGTYY WHIPTGTTQW EPPGRASPSQ GSSPQEESQL
310 320 330 340 350
TWTGFAHQEG FEEGEFWKDE PSEEAPMELG LKDPEEATLS FPAQSLSPEP
360 370 380 390 400
VPQEEEKLSQ RNANPGIKCF AVRSLGWVEM TEEELAPGRS SVAVNNCIRQ
410 420 430 440 450
LSYHKNNLHD PMAGGWGEGK DLLLQLEDET LKLVEPQNQT LLHAQPIVSI
460 470 480 490 500
RVWGVGRDSG SNRDFAYVAR DKLTQMLKCH VFRCEAPAKN IATSLHEICS
510 520 530 540 550
KIMSERRNAR CLVNGLSLDH SKLVDVPFQV EFPAPKNELV QKFQVYYLGN
560 570 580 590 600
VPVAKPVGVD VINGALESVL SSSSREQWTP SHVSVAPATL TILHQQTEAV
610 620 630 640 650
LGECRVRFLS FLAVGRDVHT FAFIMAAGPA SFCCHMFWCE PNAASLSEAV
660 670 680 690 700
QAACMLRYQK CLDARSQTST SCLPAPPAES VARRVGWTVR RGVQSLWGSL
710
KPKRLGSQTP
Length:710
Mass (Da):77,384
Last modified:July 27, 2011 - v3
Checksum:i9CFD571C50584979
GO
Isoform 2 (identifier: Q9QXJ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     462-463: Missing.

Show »
Length:708
Mass (Da):77,114
Checksum:i234B7C6D9383B148
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92T → A in AAF20141 (Ref. 1) Curated1
Sequence conflicti313E → D in AAF20141 (Ref. 1) Curated1
Sequence conflicti461 – 462SN → RE in BAC39033 (PubMed:16141072).Curated2
Sequence conflicti461 – 462SN → RE in BAE37645 (PubMed:16141072).Curated2
Sequence conflicti630A → S in BAC39033 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011659462 – 463Missing in isoform 2. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF206720 mRNA Translation: AAF20141.1
AK030748 mRNA Translation: BAC27116.1
AK083830 mRNA Translation: BAC39033.1
AK164140 mRNA Translation: BAE37645.1
AC125227 Genomic DNA No translation available.
L77865 mRNA Translation: AAB51603.1
CCDSiCCDS80775.1 [Q9QXJ1-2]
CCDS80776.1 [Q9QXJ1-1]
RefSeqiNP_001240814.1, NM_001253885.1
NP_001240815.1, NM_001253886.1
NP_033815.1, NM_009685.3
XP_006507293.1, XM_006507230.2
XP_006507294.1, XM_006507231.1
XP_017177435.1, XM_017321946.1
UniGeneiMm.38469
Mm.490092

Genome annotation databases

EnsembliENSMUST00000081165; ENSMUSP00000079932; ENSMUSG00000037032 [Q9QXJ1-1]
GeneIDi11785
KEGGimmu:11785
UCSCiuc009iyi.2 mouse [Q9QXJ1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAPBB1_MOUSE
AccessioniPrimary (citable) accession number: Q9QXJ1
Secondary accession number(s): E9QNW5
, O08642, Q3TPU0, Q8BNF4, Q8BSR9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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