UniProtKB - Q9QXG4 (ACSA_MOUSE)
Protein
Acetyl-coenzyme A synthetase, cytoplasmic
Gene
Acss2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:11150295, PubMed:16790548). Acetate is the preferred substrate but can also utilize propionate with a much lower affinity (PubMed:11150295).2 Publications
Catalytic activityi
- EC:6.2.1.12 PublicationsThis reaction proceeds in the forward2 Publications direction.
- EC:6.2.1.171 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Inhibited by acetylation at Lys-661 and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3.1 Publication
Kineticsi
- KM=0.11 mM for acetate1 Publication
- KM=3.4 mM for propionate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 363 | Coenzyme ABy similarity | 1 | |
Binding sitei | 552 | ATPBy similarity | 1 | |
Binding sitei | 567 | ATPBy similarity | 1 | |
Binding sitei | 575 | Coenzyme A; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 636 | Coenzyme ABy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 439 – 441 | ATPBy similarity | 3 | |
Nucleotide bindingi | 463 – 468 | ATPBy similarity | 6 |
GO - Molecular functioni
- acetate-CoA ligase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- propionate-CoA ligase activity Source: UniProtKB
GO - Biological processi
- acetate biosynthetic process Source: MGI
- acetyl-CoA biosynthetic process Source: MGI
- lipid biosynthetic process Source: MGI
- propionate biosynthetic process Source: MGI
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2151201, Transcriptional activation of mitochondrial biogenesis R-MMU-71384, Ethanol oxidation |
Names & Taxonomyi
Protein namesi | Recommended name: Acetyl-coenzyme A synthetase, cytoplasmic (EC:6.2.1.12 Publications)Alternative name(s): Acetate--CoA ligase Acetyl-CoA synthetase Short name: ACS Short name: AceCS Acetyl-CoA synthetase 12 Publications Short name: AceCS12 Publications Acyl-CoA synthetase short-chain family member 2 Acyl-activating enzyme Propionate--CoA ligase (EC:6.2.1.171 Publication) |
Gene namesi | Name:Acss2 Synonyms:Acas2, Acecs1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1890410, Acss2 |
Subcellular locationi
Cytosol
- cytosol 1 Publication
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Other locations
- cytoplasm Source: MGI
- intracellular membrane-bounded organelle Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000208424 | 1 – 701 | Acetyl-coenzyme A synthetase, cytoplasmicAdd BLAST | 701 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 30 | PhosphoserineCombined sources | 1 | |
Modified residuei | 263 | PhosphoserineCombined sources | 1 | |
Modified residuei | 265 | PhosphoserineCombined sources | 1 | |
Modified residuei | 267 | PhosphoserineCombined sources | 1 | |
Modified residuei | 418 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 661 | N6-acetyllysine1 Publication | 1 |
Post-translational modificationi
Reversibly acetylated at Lys-661 (PubMed:16790548). The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9QXG4 |
jPOSTi | Q9QXG4 |
MaxQBi | Q9QXG4 |
PaxDbi | Q9QXG4 |
PRIDEi | Q9QXG4 |
PTM databases
iPTMneti | Q9QXG4 |
PhosphoSitePlusi | Q9QXG4 |
SwissPalmi | Q9QXG4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027605, Expressed in brown adipose tissue and 309 other tissues |
ExpressionAtlasi | Q9QXG4, baseline and differential |
Genevisiblei | Q9QXG4, MM |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 208597, 2 interactors |
DIPi | DIP-61210N |
IntActi | Q9QXG4, 3 interactors |
STRINGi | 10090.ENSMUSP00000029135 |
Miscellaneous databases
RNActi | Q9QXG4, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 219 – 222 | Coenzyme A bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | KOG1175, Eukaryota |
GeneTreei | ENSGT00940000156358 |
HOGENOMi | CLU_000022_3_6_1 |
InParanoidi | Q9QXG4 |
OMAi | DHWWHDL |
OrthoDBi | 288915at2759 |
TreeFami | TF300417 |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR032387, ACAS_N IPR025110, AMP-bd_C IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF16177, ACAS_N, 1 hit PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9QXG4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLPEERRKS GSGSRAREET GAEGRVRGWS PPPEVRRSAH VPSLQRYREL
60 70 80 90 100
HRRSVEEPRE FWGNIAKEFY WKTACPGPFL QYNFDVTKGK IFTEWMKGAT
110 120 130 140 150
TNICYNVLDR NVHEKKLGDK VAFYWEGNEP GETTKITYRE LLVQVCQFSN
160 170 180 190 200
VLRKQGIQKG DRVAIYMPMI LELVVAMLAC ARLGALHSIV FAGFSAESLC
210 220 230 240 250
ERILDSSCCL LITTDAFYRG EKLVNLKELA DESLEKCREK GFPVRCCIVV
260 270 280 290 300
KHLGRAELGM NDSPSQSPPV KRPCPDVQIC WNEGVDLWWH ELMQQAGDEC
310 320 330 340 350
EPEWCDAEDP LFILYTSGST GKPKGVVHTI GGYMLYVATT FKYVFDFHPE
360 370 380 390 400
DVFWCTADIG WITGHSYVTY GPLANGATSV LFEGIPTYPD EGRLWSIVDK
410 420 430 440 450
YKVTKFYTAP TAIRMLMKFG DDPVTKHSRA SLQVLGTVGE PINPEAWLWY
460 470 480 490 500
HRVVGSQRCP IVDTFWQTET GGHMLTPLPG ATPMKPGSAS FPFFGVAPAI
510 520 530 540 550
LNESGEELEG EAEGYLVFKQ PWPGIMRTVY GNHTRFETTY FKKFPGYYVT
560 570 580 590 600
GDGCRRDQDG YYWITGRIDD MLNVSGHLLS TAEVESALVE HEAVAEAAVV
610 620 630 640 650
GHPHPVKGEC LYCFVTLCDG HTFSPTLTEE LKKQIREKIG PIATPDYIQN
660 670 680 690 700
APGLPKTRSG KIMRRVLRKI AQNDHDLGDT STVADPSVIN HLFSHRCLTT
Q
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AQN5 | A2AQN5_MOUSE | Propionate--CoA ligase | Acss2 | 706 | Annotation score: | ||
A2AQN4 | A2AQN4_MOUSE | Propionate--CoA ligase | Acss2 | 714 | Annotation score: | ||
F7CU63 | F7CU63_MOUSE | Acetyl-coenzyme A synthetase, cytop... | Acss2 | 293 | Annotation score: | ||
D6RHA7 | D6RHA7_MOUSE | Acetyl-coenzyme A synthetase, cytop... | Acss2 | 124 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 498 – 500 | PAI → LQS in AAF24510 (PubMed:12049778).Curated | 3 | |
Sequence conflicti | 655 – 657 | PKT → LKP in AAF24510 (PubMed:12049778).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF216873 mRNA Translation: AAF24510.1 AK053877 mRNA Translation: BAC35571.1 AL844852 Genomic DNA No translation available. BC051432 mRNA Translation: AAH51432.1 |
CCDSi | CCDS16950.1 |
RefSeqi | NP_062785.2, NM_019811.3 |
Genome annotation databases
Ensembli | ENSMUST00000029135; ENSMUSP00000029135; ENSMUSG00000027605 |
GeneIDi | 60525 |
KEGGi | mmu:60525 |
UCSCi | uc008nku.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF216873 mRNA Translation: AAF24510.1 AK053877 mRNA Translation: BAC35571.1 AL844852 Genomic DNA No translation available. BC051432 mRNA Translation: AAH51432.1 |
CCDSi | CCDS16950.1 |
RefSeqi | NP_062785.2, NM_019811.3 |
3D structure databases
SMRi | Q9QXG4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208597, 2 interactors |
DIPi | DIP-61210N |
IntActi | Q9QXG4, 3 interactors |
STRINGi | 10090.ENSMUSP00000029135 |
Chemistry databases
ChEMBLi | CHEMBL2924 |
PTM databases
iPTMneti | Q9QXG4 |
PhosphoSitePlusi | Q9QXG4 |
SwissPalmi | Q9QXG4 |
Proteomic databases
EPDi | Q9QXG4 |
jPOSTi | Q9QXG4 |
MaxQBi | Q9QXG4 |
PaxDbi | Q9QXG4 |
PRIDEi | Q9QXG4 |
Protocols and materials databases
Antibodypediai | 1332, 408 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000029135; ENSMUSP00000029135; ENSMUSG00000027605 |
GeneIDi | 60525 |
KEGGi | mmu:60525 |
UCSCi | uc008nku.1, mouse |
Organism-specific databases
CTDi | 55902 |
MGIi | MGI:1890410, Acss2 |
Phylogenomic databases
eggNOGi | KOG1175, Eukaryota |
GeneTreei | ENSGT00940000156358 |
HOGENOMi | CLU_000022_3_6_1 |
InParanoidi | Q9QXG4 |
OMAi | DHWWHDL |
OrthoDBi | 288915at2759 |
TreeFami | TF300417 |
Enzyme and pathway databases
Reactomei | R-MMU-2151201, Transcriptional activation of mitochondrial biogenesis R-MMU-71384, Ethanol oxidation |
Miscellaneous databases
BioGRID-ORCSi | 60525, 0 hits in 17 CRISPR screens |
ChiTaRSi | Acss2, mouse |
PROi | PR:Q9QXG4 |
RNActi | Q9QXG4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027605, Expressed in brown adipose tissue and 309 other tissues |
ExpressionAtlasi | Q9QXG4, baseline and differential |
Genevisiblei | Q9QXG4, MM |
Family and domain databases
Gene3Di | 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR032387, ACAS_N IPR025110, AMP-bd_C IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, AMP-dep_Synthh-like_sf |
Pfami | View protein in Pfam PF16177, ACAS_N, 1 hit PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACSA_MOUSE | |
Accessioni | Q9QXG4Primary (citable) accession number: Q9QXG4 Secondary accession number(s): Q8BK97 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2002 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families