UniProtKB - Q9QXE2 (DPOLL_MOUSE)
DNA polymerase lambda
Poll
Functioni
DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
By similarityCatalytic activityi
- EC:2.7.7.7By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 310 | Schiff-base intermediate with DNABy similarity | 1 | |
Binding sitei | 384 | dCTPBy similarity | 1 | |
Metal bindingi | 425 | ManganeseBy similarity | 1 | |
Metal bindingi | 427 | ManganeseBy similarity | 1 | |
Metal bindingi | 488 | ManganeseBy similarity | 1 | |
Binding sitei | 511 | dCTPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 415 – 418 | dCTP bindingBy similarity | 4 | |
Nucleotide bindingi | 424 – 427 | dCTP bindingBy similarity | 4 |
GO - Molecular functioni
- 5'-deoxyribose-5-phosphate lyase activity Source: UniProtKB
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- base-excision repair, gap-filling Source: UniProtKB
- DNA biosynthetic process Source: MGI
- DNA replication Source: UniProtKB-KW
- double-strand break repair via homologous recombination Source: UniProtKB
- double-strand break repair via nonhomologous end joining Source: UniProtKB
- nucleotide-excision repair Source: UniProtKB
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Lyase, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication, DNA synthesis |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-5693571, Nonhomologous End-Joining (NHEJ) |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1889000, Poll |
VEuPathDBi | HostDB:ENSMUSG00000025218 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- site of double-strand break Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218785 | 1 – 573 | DNA polymerase lambdaAdd BLAST | 573 |
Proteomic databases
EPDi | Q9QXE2 |
MaxQBi | Q9QXE2 |
PaxDbi | Q9QXE2 |
PeptideAtlasi | Q9QXE2 |
PRIDEi | Q9QXE2 |
ProteomicsDBi | 277390 |
PTM databases
iPTMneti | Q9QXE2 |
PhosphoSitePlusi | Q9QXE2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000025218, Expressed in spermatocyte and 225 other tissues |
Genevisiblei | Q9QXE2, MM |
Interactioni
Subunit structurei
Interacts with PCNA.
Interacts with PAXX; promoting POLL recruitment to double-strand breaks (DSBs) and stimulation of the end-filling activity of POLL.
Interacts with XRCC4; promoting POLL recruitment to double-strand breaks (DSBs) and stimulation of the end-filling activity of POLL.
Interacts with NHEJ1/XLF; promoting POLL recruitment to double-strand breaks (DSBs) and stimulation of the end-filling activity of POLL.
By similarityProtein-protein interaction databases
BioGRIDi | 208108, 1 interactor |
STRINGi | 10090.ENSMUSP00000026239 |
Chemistry databases
BindingDBi | Q9QXE2 |
Miscellaneous databases
RNActi | Q9QXE2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 131 | BRCTPROSITE-ProRule annotationAdd BLAST | 97 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 126 – 204 | DisorderedSequence analysisAdd BLAST | 79 | |
Regioni | 214 – 233 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 263 – 277 | DNA-bindingBy similarityAdd BLAST | 15 | |
Regioni | 343 – 346 | DNA-bindingBy similarity | 4 | |
Regioni | 418 – 427 | Involved in primer bindingBy similarity | 10 | |
Regioni | 464 – 503 | DNA-bindingBy similarityAdd BLAST | 40 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 141 – 170 | Polar residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 180 – 204 | Polar residuesSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2534, Eukaryota |
GeneTreei | ENSGT00940000158515 |
HOGENOMi | CLU_008698_6_1_1 |
InParanoidi | Q9QXE2 |
OMAi | KWHGASA |
OrthoDBi | 1328151at2759 |
PhylomeDBi | Q9QXE2 |
TreeFami | TF103011 |
Family and domain databases
CDDi | cd00141, NT_POLXc, 1 hit |
Gene3Di | 1.10.150.110, 1 hit 3.30.210.10, 1 hit 3.30.460.10, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR002054, DNA-dir_DNA_pol_X IPR019843, DNA_pol-X_BS IPR010996, DNA_pol_b-like_N IPR028207, DNA_pol_B_palm_palm IPR018944, DNA_pol_lambd_fingers_domain IPR027421, DNA_pol_lamdba_lyase_dom_sf IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR002008, DNA_pol_X_beta-like IPR043519, NT_sf IPR029398, PolB_thumb |
PANTHERi | PTHR11276, PTHR11276, 1 hit |
Pfami | View protein in Pfam PF14792, DNA_pol_B_palm, 1 hit PF14791, DNA_pol_B_thumb, 1 hit PF10391, DNA_pol_lambd_f, 1 hit PF14716, HHH_8, 1 hit |
PRINTSi | PR00869, DNAPOLX PR00870, DNAPOLXBETA |
SMARTi | View protein in SMART SM00483, POLXc, 1 hit |
SUPFAMi | SSF47802, SSF47802, 1 hit SSF52113, SSF52113, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit PS00522, DNA_POLYMERASE_X, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDPQGIVKAF PKRKKSHADL SSKALAKIPK REVGEARGWL SSLRAHIMPA
60 70 80 90 100
GIGRARAELF EKQIIHHGGQ VCSAQAPGVT HIVVDEDMDY ERALRLLRLP
110 120 130 140 150
QLPPGAQLVK STWLSLCLQE GRLTDTEGFS LPMPKRSLDE PQPSKSGQDA
160 170 180 190 200
SAPGTQRDLP RTTLSLSPPH TRAVSPPPTA EKPSRTQAQL SSEDETSDGE
210 220 230 240 250
GPQVSSADLQ ALITGHYPTP PEEDGGPDPA PEALDKWVCA QPSSQKATNY
260 270 280 290 300
NLHITEKLEV LAKAYSVQGD KWRALGYAKA INALKSFHKP VSSYQEACSI
310 320 330 340 350
PGIGKRMAEK VMEILESGHL RKLDHISDSV PVLELFSNIW GAGTKTAQMW
360 370 380 390 400
YHQGFRNLED LQSLGSLTAQ QAIGLKHYDD FLDRMPREEA AEIEQTVRIS
410 420 430 440 450
AQAFNPGLLC VACGSYRRGK MTCGDVDVLI THPDGRSHRG IFSCLLDSLR
460 470 480 490 500
QQGFLTDDLV SQEENGQQQK YLGVCRLPGP GKRHRRLDII VVPYCEFACA
510 520 530 540 550
LLYFTGSAHF NRSMRALAKT KGMSLSEHAL SAAVVRNSQG VKVGPGQVLP
560 570
TPTEKDVFKH LGLPYREPAE RDW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ131889 mRNA Translation: CAB65241.1 AF176099 mRNA Translation: AAF27553.1 BC004767 mRNA Translation: AAH04767.1 AK003392 mRNA Translation: BAB22759.1 |
CCDSi | CCDS29861.1 |
RefSeqi | NP_001317435.1, NM_001330506.1 NP_001317436.1, NM_001330507.1 NP_064416.1, NM_020032.3 XP_011245603.1, XM_011247301.2 |
Genome annotation databases
Ensembli | ENSMUST00000026239; ENSMUSP00000026239; ENSMUSG00000025218 |
GeneIDi | 56626 |
KEGGi | mmu:56626 |
UCSCi | uc008hrc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ131889 mRNA Translation: CAB65241.1 AF176099 mRNA Translation: AAF27553.1 BC004767 mRNA Translation: AAH04767.1 AK003392 mRNA Translation: BAB22759.1 |
CCDSi | CCDS29861.1 |
RefSeqi | NP_001317435.1, NM_001330506.1 NP_001317436.1, NM_001330507.1 NP_064416.1, NM_020032.3 XP_011245603.1, XM_011247301.2 |
3D structure databases
AlphaFoldDBi | Q9QXE2 |
SMRi | Q9QXE2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208108, 1 interactor |
STRINGi | 10090.ENSMUSP00000026239 |
Chemistry databases
BindingDBi | Q9QXE2 |
ChEMBLi | CHEMBL3124737 |
PTM databases
iPTMneti | Q9QXE2 |
PhosphoSitePlusi | Q9QXE2 |
Proteomic databases
EPDi | Q9QXE2 |
MaxQBi | Q9QXE2 |
PaxDbi | Q9QXE2 |
PeptideAtlasi | Q9QXE2 |
PRIDEi | Q9QXE2 |
ProteomicsDBi | 277390 |
Protocols and materials databases
Antibodypediai | 31295, 245 antibodies from 32 providers |
DNASUi | 56626 |
Genome annotation databases
Ensembli | ENSMUST00000026239; ENSMUSP00000026239; ENSMUSG00000025218 |
GeneIDi | 56626 |
KEGGi | mmu:56626 |
UCSCi | uc008hrc.1, mouse |
Organism-specific databases
CTDi | 27343 |
MGIi | MGI:1889000, Poll |
VEuPathDBi | HostDB:ENSMUSG00000025218 |
Phylogenomic databases
eggNOGi | KOG2534, Eukaryota |
GeneTreei | ENSGT00940000158515 |
HOGENOMi | CLU_008698_6_1_1 |
InParanoidi | Q9QXE2 |
OMAi | KWHGASA |
OrthoDBi | 1328151at2759 |
PhylomeDBi | Q9QXE2 |
TreeFami | TF103011 |
Enzyme and pathway databases
Reactomei | R-MMU-5693571, Nonhomologous End-Joining (NHEJ) |
Miscellaneous databases
BioGRID-ORCSi | 56626, 9 hits in 106 CRISPR screens |
ChiTaRSi | Polk, mouse |
PROi | PR:Q9QXE2 |
RNActi | Q9QXE2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025218, Expressed in spermatocyte and 225 other tissues |
Genevisiblei | Q9QXE2, MM |
Family and domain databases
CDDi | cd00141, NT_POLXc, 1 hit |
Gene3Di | 1.10.150.110, 1 hit 3.30.210.10, 1 hit 3.30.460.10, 1 hit 3.40.50.10190, 1 hit |
InterProi | View protein in InterPro IPR001357, BRCT_dom IPR036420, BRCT_dom_sf IPR002054, DNA-dir_DNA_pol_X IPR019843, DNA_pol-X_BS IPR010996, DNA_pol_b-like_N IPR028207, DNA_pol_B_palm_palm IPR018944, DNA_pol_lambd_fingers_domain IPR027421, DNA_pol_lamdba_lyase_dom_sf IPR037160, DNA_Pol_thumb_sf IPR022312, DNA_pol_X IPR002008, DNA_pol_X_beta-like IPR043519, NT_sf IPR029398, PolB_thumb |
PANTHERi | PTHR11276, PTHR11276, 1 hit |
Pfami | View protein in Pfam PF14792, DNA_pol_B_palm, 1 hit PF14791, DNA_pol_B_thumb, 1 hit PF10391, DNA_pol_lambd_f, 1 hit PF14716, HHH_8, 1 hit |
PRINTSi | PR00869, DNAPOLX PR00870, DNAPOLXBETA |
SMARTi | View protein in SMART SM00483, POLXc, 1 hit |
SUPFAMi | SSF47802, SSF47802, 1 hit SSF52113, SSF52113, 1 hit SSF81301, SSF81301, 1 hit |
PROSITEi | View protein in PROSITE PS50172, BRCT, 1 hit PS00522, DNA_POLYMERASE_X, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOLL_MOUSE | |
Accessioni | Q9QXE2Primary (citable) accession number: Q9QXE2 Secondary accession number(s): Q9CTJ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families