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Protein

Fructose-1,6-bisphosphatase 1

Gene

Fbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.3 Publications

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Activity regulationi

Subject to complex allosteric regulation. The enzyme can assume an active R-state, or an inactive T-state. Intermediate conformations may exist. AMP acts as allosteric inhibitor. AMP binding affects the turnover of bound substrate and not the affinity for substrate. Fructose 2,6-bisphosphate acts as competitive inhibitor. Fructose 2,6-bisphosphate and AMP have synergistic effects (By similarity).By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Magnesium 1By similarity1
Metal bindingi98Magnesium 1By similarity1
Metal bindingi98Magnesium 2By similarity1
Metal bindingi119Magnesium 2By similarity1
Metal bindingi119Magnesium 3By similarity1
Metal bindingi121Magnesium 2; via carbonyl oxygenBy similarity1
Metal bindingi122Magnesium 3By similarity1
Binding sitei141AMPBy similarity1
Binding sitei265SubstrateBy similarity1
Metal bindingi281Magnesium 3By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 22AMPBy similarity5
Nucleotide bindingi28 – 32AMPBy similarity5
Nucleotide bindingi113 – 114AMPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Hydrolase
Biological processCarbohydrate metabolism, Gluconeogenesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis
UniPathwayi
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphatase 1 (EC:3.1.3.11By similarity)
Short name:
FBPase 1
Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase 1
Fructose-1,6-bisphosphatase isozyme 3
Short name:
FBPase 3
Liver FBPase
Gene namesi
Name:Fbp1
Synonyms:Fbp, Fbp3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:95492 Fbp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5360

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002004992 – 338Fructose-1,6-bisphosphatase 1Add BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei151N6-succinyllysineCombined sources1
Modified residuei216PhosphotyrosineCombined sources1
Modified residuei245PhosphotyrosineCombined sources1
Modified residuei265PhosphotyrosineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9QXD6
PaxDbiQ9QXD6
PeptideAtlasiQ9QXD6
PRIDEiQ9QXD6

2D gel databases

REPRODUCTION-2DPAGEiIPI00228630
Q9QXD6

PTM databases

iPTMnetiQ9QXD6
PhosphoSitePlusiQ9QXD6
SwissPalmiQ9QXD6

Expressioni

Tissue specificityi

Detected in pancreatic beta-cell lines MIN6 and beta-TC and in liver (at protein level). Preferentially expressed in liver, with lower levels detected in pancreatic islets and intestine, and very low levels in blood, muscle, brain and spleen.4 Publications

Inductioni

Up-regulated in pancreas by obesity and dietary fat intake and in diabetic animals.1 Publication

Gene expression databases

BgeeiENSMUSG00000069805 Expressed in 147 organ(s), highest expression level in adult mammalian kidney
CleanExiMM_FBP1
ExpressionAtlasiQ9QXD6 baseline and differential
GenevisibleiQ9QXD6 MM

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199610, 1 interactor
IntActiQ9QXD6, 18 interactors
MINTiQ9QXD6
STRINGi10090.ENSMUSP00000090564

Chemistry databases

BindingDBiQ9QXD6

Structurei

3D structure databases

ProteinModelPortaliQ9QXD6
SMRiQ9QXD6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 125Substrate bindingBy similarity4
Regioni213 – 216Substrate bindingBy similarity4
Regioni244 – 249Substrate bindingBy similarity6
Regioni275 – 277Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the FBPase class 1 family.Curated

Phylogenomic databases

eggNOGiKOG1458 Eukaryota
COG0158 LUCA
GeneTreeiENSGT00390000015513
HOGENOMiHOG000191265
HOVERGENiHBG005627
InParanoidiQ9QXD6
KOiK03841
OMAiQSGLVCR
OrthoDBiEOG091G0AZP
PhylomeDBiQ9QXD6
TreeFamiTF314824

Family and domain databases

CDDicd00354 FBPase, 1 hit
HAMAPiMF_01855 FBPase_class1, 1 hit
InterProiView protein in InterPro
IPR000146 FBPase_class-1
IPR033391 FBPase_N
IPR028343 FBPtase
IPR020548 Fructose_bisphosphatase_AS
PANTHERiPTHR11556 PTHR11556, 1 hit
PfamiView protein in Pfam
PF00316 FBPase, 1 hit
PIRSFiPIRSF500210 FBPtase, 1 hit
PIRSF000904 FBPtase_SBPase, 1 hit
PRINTSiPR00115 F16BPHPHTASE
PROSITEiView protein in PROSITE
PS00124 FBPASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9QXD6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MANHAPFETD ISTLTRFVME QGRKAQGTGE LTQLLNSLCT AIKAISSAVR
60 70 80 90 100
QAGIAQLYGI AGSTNVTGDQ VKKLDILSND LVINMLKSSY ATCVLVSEEN
110 120 130 140 150
TNAIIIEPEK RGKYVVCFDP LDGSSNIDCL VSIGTIFGIY RKKSTDEPSE
160 170 180 190 200
KDALQPGRDL VAAGYALYGS ATMLVLAMDC GVNCFMLDPS IGEFIMVDRD
210 220 230 240 250
VKMKKKGNIY SLNEGYAKDF DPAINEYLQR KKFPPDGSAP YGARYVGSMV
260 270 280 290 300
ADIHRTLVYG GIFLYPANKK SPSGKLRLLY ECNPIAYVME KAGGLATTGD
310 320 330
KDILDIVPTE IHQKAPVVMG SSEDVQEFLE IYRKHKAK
Length:338
Mass (Da):36,912
Last modified:January 23, 2007 - v3
Checksum:i50AE999F0D517E4F
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9Q0T7E9Q0T7_MOUSE
Fructose-1,6-bisphosphatase 1
Fbp1
57Annotation score:
F6YS63F6YS63_MOUSE
Fructose-1,6-bisphosphatase 1
Fbp1
133Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198D → E in CAA71946 (PubMed:9106734).Curated1
Sequence conflicti201V → G in CAA71946 (PubMed:9106734).Curated1
Sequence conflicti221D → N in CAA71946 (PubMed:9106734).Curated1
Sequence conflicti225N → L in CAA71946 (PubMed:9106734).Curated1
Sequence conflicti228L → H in CAA71946 (PubMed:9106734).Curated1
Sequence conflicti231K → Q in CAA71946 (PubMed:9106734).Curated1
Sequence conflicti234 – 235PP → HE in CAA71946 (PubMed:9106734).Curated2
Sequence conflicti238S → T in CAA71946 (PubMed:9106734).Curated1
Sequence conflicti243A → T in CAA71946 (PubMed:9106734).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132693 mRNA Translation: CAB65244.1
AK002216 mRNA Translation: BAB21941.1
AK149527 mRNA Translation: BAE28940.1
BC011480 mRNA Translation: AAH11480.1
BC051392 mRNA Translation: AAH51392.1
Y11067 mRNA Translation: CAA71946.1
CCDSiCCDS26590.1
RefSeqiNP_062268.1, NM_019395.3
UniGeneiMm.423078

Genome annotation databases

EnsembliENSMUST00000092888; ENSMUSP00000090564; ENSMUSG00000069805
GeneIDi14121
KEGGimmu:14121
UCSCiuc007qxg.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132693 mRNA Translation: CAB65244.1
AK002216 mRNA Translation: BAB21941.1
AK149527 mRNA Translation: BAE28940.1
BC011480 mRNA Translation: AAH11480.1
BC051392 mRNA Translation: AAH51392.1
Y11067 mRNA Translation: CAA71946.1
CCDSiCCDS26590.1
RefSeqiNP_062268.1, NM_019395.3
UniGeneiMm.423078

3D structure databases

ProteinModelPortaliQ9QXD6
SMRiQ9QXD6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199610, 1 interactor
IntActiQ9QXD6, 18 interactors
MINTiQ9QXD6
STRINGi10090.ENSMUSP00000090564

Chemistry databases

BindingDBiQ9QXD6
ChEMBLiCHEMBL5360

PTM databases

iPTMnetiQ9QXD6
PhosphoSitePlusiQ9QXD6
SwissPalmiQ9QXD6

2D gel databases

REPRODUCTION-2DPAGEiIPI00228630
Q9QXD6

Proteomic databases

MaxQBiQ9QXD6
PaxDbiQ9QXD6
PeptideAtlasiQ9QXD6
PRIDEiQ9QXD6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092888; ENSMUSP00000090564; ENSMUSG00000069805
GeneIDi14121
KEGGimmu:14121
UCSCiuc007qxg.2 mouse

Organism-specific databases

CTDi2203
MGIiMGI:95492 Fbp1

Phylogenomic databases

eggNOGiKOG1458 Eukaryota
COG0158 LUCA
GeneTreeiENSGT00390000015513
HOGENOMiHOG000191265
HOVERGENiHBG005627
InParanoidiQ9QXD6
KOiK03841
OMAiQSGLVCR
OrthoDBiEOG091G0AZP
PhylomeDBiQ9QXD6
TreeFamiTF314824

Enzyme and pathway databases

UniPathwayi
UPA00138

ReactomeiR-MMU-70263 Gluconeogenesis

Miscellaneous databases

ChiTaRSiFbp1 mouse
PROiPR:Q9QXD6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000069805 Expressed in 147 organ(s), highest expression level in adult mammalian kidney
CleanExiMM_FBP1
ExpressionAtlasiQ9QXD6 baseline and differential
GenevisibleiQ9QXD6 MM

Family and domain databases

CDDicd00354 FBPase, 1 hit
HAMAPiMF_01855 FBPase_class1, 1 hit
InterProiView protein in InterPro
IPR000146 FBPase_class-1
IPR033391 FBPase_N
IPR028343 FBPtase
IPR020548 Fructose_bisphosphatase_AS
PANTHERiPTHR11556 PTHR11556, 1 hit
PfamiView protein in Pfam
PF00316 FBPase, 1 hit
PIRSFiPIRSF500210 FBPtase, 1 hit
PIRSF000904 FBPtase_SBPase, 1 hit
PRINTSiPR00115 F16BPHPHTASE
PROSITEiView protein in PROSITE
PS00124 FBPASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiF16P1_MOUSE
AccessioniPrimary (citable) accession number: Q9QXD6
Secondary accession number(s): P97323, Q3UEH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 152 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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