UniProtKB - Q9QX74 (SHAN2_RAT)
SH3 and multiple ankyrin repeat domains protein 2
Shank2
Functioni
GO - Molecular functioni
- ionotropic glutamate receptor binding Source: BHF-UCL
- protein C-terminus binding Source: RGD
- SH3 domain binding Source: RGD
- signaling receptor complex adaptor activity Source: GO_Central
- structural constituent of postsynaptic density Source: SynGO
- synaptic receptor adaptor activity Source: GO_Central
GO - Biological processi
- adult behavior Source: BHF-UCL
- brain development Source: RGD
- brain morphogenesis Source: GO_Central
- dendritic spine morphogenesis Source: GO_Central
- exploration behavior Source: BHF-UCL
- learning Source: BHF-UCL
- long-term synaptic depression Source: BHF-UCL
- long-term synaptic potentiation Source: BHF-UCL
- maintenance of postsynaptic density structure Source: RGD
- memory Source: BHF-UCL
- positive regulation of dendritic spine development Source: GO_Central
- positive regulation of excitatory postsynaptic potential Source: GO_Central
- positive regulation of synaptic transmission, glutamatergic Source: RGD
- postsynaptic density assembly Source: GO_Central
- regulation of AMPA receptor activity Source: GO_Central
- regulation of synapse organization Source: SynGO
- response to nutrient Source: RGD
- social behavior Source: BHF-UCL
- synapse assembly Source: BHF-UCL
- synaptic growth at neuromuscular junction Source: GO_Central
- vocalization behavior Source: BHF-UCL
Enzyme and pathway databases
Reactomei | R-RNO-6794361, Neurexins and neuroligins |
Names & Taxonomyi
Protein namesi | Recommended name: SH3 and multiple ankyrin repeat domains protein 2Short name: Shank2 Alternative name(s): Cortactin-binding protein 1 Short name: CortBP1 GKAP/SAPAP-interacting protein Proline-rich synapse-associated protein 1 Short name: ProSAP1 SPANK-3 |
Gene namesi | Name:Shank2 Synonyms:Cortbp1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628772, Shank2 |
Subcellular locationi
Plasma membrane
- Apical cell membrane 1 Publication
Other locations
- Cytoplasm 1 Publication
- synapse 1 Publication
- postsynaptic density 1 Publication
- growth cone 1 Publication
- dendritic spine By similarity
Note: Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Present in the dendritic spines of cerebellar Purkinje cells (By similarity). Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells.By similarity
Cytoskeleton
- neurofilament Source: RGD
Plasma Membrane
- apical plasma membrane Source: UniProtKB
- brush border membrane Source: RGD
- ciliary membrane Source: BHF-UCL
- plasma membrane Source: BHF-UCL
Other locations
- dendrite Source: GO_Central
- dendritic spine Source: BHF-UCL
- glutamatergic synapse Source: SynGO
- growth cone Source: RGD
- neuron projection Source: BHF-UCL
- neuron spine Source: GO_Central
- neuronal cell body Source: RGD
- photoreceptor inner segment Source: BHF-UCL
- photoreceptor outer segment Source: BHF-UCL
- postsynaptic density Source: SynGO
- presynaptic active zone Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174674 | 1 – 1474 | SH3 and multiple ankyrin repeat domains protein 2Add BLAST | 1474 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 457 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 486 | PhosphothreonineBy similarity | 1 | ||
Modified residuei | 586 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 724 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 903 | PhosphothreonineBy similarity | 1 | ||
Glycosylationi | 1292 | O-linked (GlcNAc) threonineBy similarity | 1 | ||
Modified residuei | 1334 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 1338 | PhosphoserineBy similarity | 1 | ||
Isoform 4 (identifier: Q9QX74-4) | |||||
Modified residuei | 162 | PhosphoserineCombined sources | 1 | ||
Isoform 5 (identifier: Q9QX74-5) | |||||
Modified residuei | 162 | PhosphoserineCombined sources | 1 | ||
Isoform 6 (identifier: Q9QX74-6) | |||||
Modified residuei | 162 | PhosphoserineCombined sources | 1 | ||
Isoform 2 (identifier: Q9QX74-2) | |||||
Modified residuei | 373 | PhosphoserineCombined sources | 1 | ||
Isoform 3 (identifier: Q9QX74-3) | |||||
Modified residuei | 373 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q9QX74 |
PaxDbi | Q9QX74 |
PRIDEi | Q9QX74 |
PTM databases
GlyGeni | Q9QX74, 1 site |
iPTMneti | Q9QX74 |
PhosphoSitePlusi | Q9QX74 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSRNOG00000050206, Expressed in frontal cortex and 18 other tissues |
ExpressionAtlasi | Q9QX74, baseline and differential |
Genevisiblei | Q9QX74, RN |
Interactioni
Subunit structurei
Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP.
Interacts with CTTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1.
Interacts with DNM2, DBNL, GRID2, BAIAP2, SLC9A3, PLCB3 and CFTR.
Interacts with ABI1 (via SH3 domain).
Interacts (via proline-rich region) with PDE4D isoform 5 (via N-terminal region).
Interacts with PDE4D isoform 33, isoform 4, isoform 7, isoform 8 and isoform 9 but not isoform 32 and isoform 6.
Interacts weakly with PDE4D isoform 31.
Interacts with ABI1.
9 PublicationsBinary interactionsi
Hide detailsQ9QX74
Isoform 7 [Q9QX74-7]
With | #Exp. | IntAct |
---|---|---|
Cacna1d [P27732] | 2 | EBI-20939146,EBI-8072674 |
GO - Molecular functioni
- ionotropic glutamate receptor binding Source: BHF-UCL
- protein C-terminus binding Source: RGD
- SH3 domain binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 251104, 4 interactors |
CORUMi | Q9QX74 |
IntActi | Q9QX74, 12 interactors |
MINTi | Q9QX74 |
STRINGi | 10116.ENSRNOP00000067841 |
Structurei
Secondary structure
3D structure databases
SMRi | Q9QX74 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 148 – 207 | SH3PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 248 – 342 | PDZPROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 1411 – 1474 | SAMPROSITE-ProRule annotationAdd BLAST | 64 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1169 – 1175 | SH3-bindingSequence analysis | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 24 – 27 | Poly-Arg | 4 | |
Compositional biasi | 521 – 529 | Poly-Pro | 9 | |
Compositional biasi | 1203 – 1211 | Poly-Pro | 9 |
Domaini
Sequence similaritiesi
Keywords - Domaini
SH3 domain, SH3-bindingPhylogenomic databases
eggNOGi | KOG0504, Eukaryota KOG4375, Eukaryota |
GeneTreei | ENSGT00940000153561 |
InParanoidi | Q9QX74 |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR001478, PDZ IPR041489, PDZ_6 IPR036034, PDZ_sf IPR001660, SAM IPR013761, SAM/pointed_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF17820, PDZ_6, 1 hit PF00536, SAM_1, 1 hit PF07653, SH3_2, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit SM00454, SAM, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50106, PDZ, 1 hit PS50105, SAM_DOMAIN, 1 hit PS50002, SH3, 1 hit |
s (7+)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 7 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKSLLNAFTK KEVPFREAPA YSNRRRRPPN TLAAPRVLLR SNSDNNLNAG
60 70 80 90 100
APEWAVCSAA TSHRSLSPQL LQQTPSKPDG ATKSLGSYAP GPRSRSPSLN
110 120 130 140 150
RLGGAGEDGK RPQPPHWHVG SPFTPGANKD SLSTFEYPGP RRKLYSAVPG
160 170 180 190 200
RLFVAIKPYQ PQVDGEIPLH RGDRVKVLSI GEGGFWEGSA RGHIGWFPAE
210 220 230 240 250
CVEEVQCKPR DSQAETRADR SKKLFRHYTV GSYDSFDAAS DCIIEDKTVV
260 270 280 290 300
LQKKDNEGFG FVLRGAKADT PIEEFTPTPA FPALQYLESV DEGGVAWQAG
310 320 330 340 350
LRTGDFLIEV NNENVVKVGH RQVVNMIRQG GNHLVLKVVT VTRNLDPDDT
360 370 380 390 400
ARKKAPPPPK RAPTTALTLR SKSMTAELEE LGLSLVDKAS VRKKKDKPEE
410 420 430 440 450
IVPASKPSRT AENVAIESRV ATIKQRPTSR CFPAASDVNS VYERQGIAVM
460 470 480 490 500
TPTVPGSPKG PFLGLPRGTM RRQKSIDSRI FLSGITEEER QFLAPPMLKF
510 520 530 540 550
TRSLSMPDTS EDIPPPPQSV PPSPPPPSPT TYNCPRSPTP RVYGTIKPAF
560 570 580 590 600
NQNPVAKVPP ATRSDTVATM MREKGMFYRR ELDRFSLDSE DVYSRSPAPQ
610 620 630 640 650
AAFRTKRGQM PENPYSEVGK IASKAVYVPA KPARRKGMLV KQSNVEDSPE
660 670 680 690 700
KTCSIPIPTI IVKEPSTSSS GKSSQGSSME IDPQATEPGQ LRPDDSLTVS
710 720 730 740 750
SPFAAAIAGA VRDREKRLEA RRNSPAFLST DLGDEDVGLG PPAPRMQPSK
760 770 780 790 800
FPEEGGFGDE DETEQPLLPT PGAAPRELEN HFLGGGEAGA QGEAGGPLSS
810 820 830 840 850
TSKAKGPESG PAAALKSSSP ASPENYVHPL TGRLLDPSSP LALALSARDR
860 870 880 890 900
AMQESQQGHK GEAPKADLNK PLYIDTKMRP SVESGFPPVT RQNTRGPLRR
910 920 930 940 950
QETENKYETD LSKDRRADDK KNMLINIVDT AQQKSAGLLM VHTVDIPVAG
960 970 980 990 1000
PPLEEEEDRE DGDTKPDHSP STVPEGVPKT EGALQISAAP EPAAAPGRTI
1010 1020 1030 1040 1050
VAAGSVEEAV ILPFRIPPPP LASVDLDEDF LFTEPLPPPL EFANSFDIPD
1060 1070 1080 1090 1100
DRAASVPALA DLVKQKKSDT PQPPSLNSSQ PANSTDSKKP AGISNCLPSS
1110 1120 1130 1140 1150
FLPPPESFDA VTDSGIEEVD SRSSSDHHLE TTSTISTVSS ISTLSSEGGE
1160 1170 1180 1190 1200
SMDTCTVYAD GQAFVVDKPP VPPKPKMKPI VHKSNALYQD TLPEEDTDGF
1210 1220 1230 1240 1250
VIPPPAPPPP PGSAQAGVAK VIQPRTSKLW GDVTEVKSPI LSGPKANVIS
1260 1270 1280 1290 1300
ELNSILQQMN RGKSVKPGEG LELPVGAKSA NLAPRSPEVM STVSGTRSTT
1310 1320 1330 1340 1350
VTFTVRPGTS QPITLQSRPP DYESRTSGPR RAPSPVVSPT ELSKEILPTP
1360 1370 1380 1390 1400
PSAAAASPSP TLSDVFSLPS QSPAGDLFGL NPAGRSRSPS PSILQQPISN
1410 1420 1430 1440 1450
KPFTTKPVHL WTKPDVADWL ESLNLGEHKE TFMDNEIDGS HLPNLQKEDL
1460 1470
IDLGVTRVGH RMNIERALKQ LLDR
The sequence of this isoform differs from the canonical sequence as follows:
1-12: MKSLLNAFTKKE → MPRSPTSSED...YIKNHKETDI
382-395: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0R5T5 | M0R5T5_RAT | SH3 and multiple ankyrin repeat dom... | Shank2 | 1,842 | Annotation score: | ||
A0A0U1RRR2 | A0A0U1RRR2_RAT | SH3 and multiple ankyrin repeat dom... | Shank2 | 353 | Annotation score: | ||
A0A0U1RS17 | A0A0U1RS17_RAT | SH3 and multiple ankyrin repeat dom... | Shank2 | 589 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 – 41 | PRVLLRS → QKNLGAA (PubMed:10506216).Curated | 7 | |
Sequence conflicti | 152 | L → F in AAF02497 (PubMed:14977424).Curated | 1 | |
Sequence conflicti | 369 | L → V in AAC62226 (PubMed:9742101).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006081 | 1 – 211 | Missing in isoform 4, isoform 5 and isoform 6. 2 PublicationsAdd BLAST | 211 | |
Alternative sequenceiVSP_020049 | 1 – 12 | MKSLL…FTKKE → MPRSPTSSEDEMAQSFSDYS VGSESDSSKEETIYDTIRAT TEKPGGVKMEDLQGNTLVIR VVIQDLQQTKCIRFNPDATV WVAKQRILCTLNQGLKDVLN YGLFQPASNGRDGKFLDEER LLREYPQPMGQGVPSLEFRY KKRVYKQSNLDEKQLARLHT KTNLKKFMDHTQHRSVEKLV KLLDRGLDPNFHDLETGETP LTLAAQLDGSMEVIKALRNG GAHLDFRSRDGMTALHKAAR MRNQVALKTLLELGASPDYK DSYGLTPLYHTAIVGGDPYC CELLLHEHASVCCKDENGWH EIHQACRYGHVQHLEHLLFY GADMSAQNASGNTALHICAL YNQDSCARVLLFRGGDKELK NYNSQTPFQVAIIAGNFELA EYIKNHKETDI in isoform 7. 1 PublicationAdd BLAST | 12 | |
Alternative sequenceiVSP_006082 | 212 – 239 | SQAET…SFDAA → MMSVPGGGAATVMMTGYNNG RYPRNSLY in isoform 4, isoform 5 and isoform 6. 2 PublicationsAdd BLAST | 28 | |
Alternative sequenceiVSP_020050 | 382 – 395 | Missing in isoform 7. 1 PublicationAdd BLAST | 14 | |
Alternative sequenceiVSP_006083 | 382 – 385 | Missing in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6. 3 Publications | 4 | |
Alternative sequenceiVSP_006084 | 388 – 394 | Missing in isoform 3 and isoform 5. 3 Publications | 7 | |
Alternative sequenceiVSP_006086 | 468 – 483 | Missing in isoform 3. 1 PublicationAdd BLAST | 16 | |
Alternative sequenceiVSP_006085 | 468 – 476 | Missing in isoform 6. 1 Publication | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060116 mRNA Translation: AAC62226.1 AJ249562 mRNA Translation: CAB56522.1 AJ131899 mRNA Translation: CAB44314.1 AJ131899 mRNA Translation: CAB44312.1 AJ131899 mRNA Translation: CAB44313.1 AY298755 mRNA Translation: AAP85236.1 AF141903 mRNA Translation: AAF02497.1 AF159048 mRNA Translation: AAD42977.1 Frameshift. |
PIRi | T14272 |
RefSeqi | NP_001004133.1, NM_001004133.1 NP_597684.1, NM_133440.1 [Q9QX74-4] NP_597685.1, NM_133441.1 [Q9QX74-2] NP_958738.1, NM_201350.1 [Q9QX74-7] XP_017444213.1, XM_017588724.1 [Q9QX74-7] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060116 mRNA Translation: AAC62226.1 AJ249562 mRNA Translation: CAB56522.1 AJ131899 mRNA Translation: CAB44314.1 AJ131899 mRNA Translation: CAB44312.1 AJ131899 mRNA Translation: CAB44313.1 AY298755 mRNA Translation: AAP85236.1 AF141903 mRNA Translation: AAF02497.1 AF159048 mRNA Translation: AAD42977.1 Frameshift. |
PIRi | T14272 |
RefSeqi | NP_001004133.1, NM_001004133.1 NP_597684.1, NM_133440.1 [Q9QX74-4] NP_597685.1, NM_133441.1 [Q9QX74-2] NP_958738.1, NM_201350.1 [Q9QX74-7] XP_017444213.1, XM_017588724.1 [Q9QX74-7] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6CPJ | NMR | - | A | 148-206 | [»] | |
SMRi | Q9QX74 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 251104, 4 interactors |
CORUMi | Q9QX74 |
IntActi | Q9QX74, 12 interactors |
MINTi | Q9QX74 |
STRINGi | 10116.ENSRNOP00000067841 |
PTM databases
GlyGeni | Q9QX74, 1 site |
iPTMneti | Q9QX74 |
PhosphoSitePlusi | Q9QX74 |
Proteomic databases
jPOSTi | Q9QX74 |
PaxDbi | Q9QX74 |
PRIDEi | Q9QX74 |
Protocols and materials databases
ABCDi | Q9QX74, 2 sequenced antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 22941 |
RGDi | 628772, Shank2 |
Phylogenomic databases
eggNOGi | KOG0504, Eukaryota KOG4375, Eukaryota |
GeneTreei | ENSGT00940000153561 |
InParanoidi | Q9QX74 |
Enzyme and pathway databases
Reactomei | R-RNO-6794361, Neurexins and neuroligins |
Miscellaneous databases
PROi | PR:Q9QX74 |
Gene expression databases
Bgeei | ENSRNOG00000050206, Expressed in frontal cortex and 18 other tissues |
ExpressionAtlasi | Q9QX74, baseline and differential |
Genevisiblei | Q9QX74, RN |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR001478, PDZ IPR041489, PDZ_6 IPR036034, PDZ_sf IPR001660, SAM IPR013761, SAM/pointed_sf IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF17820, PDZ_6, 1 hit PF00536, SAM_1, 1 hit PF07653, SH3_2, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit SM00454, SAM, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF50044, SSF50044, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50106, PDZ, 1 hit PS50105, SAM_DOMAIN, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SHAN2_RAT | |
Accessioni | Q9QX74Primary (citable) accession number: Q9QX74 Secondary accession number(s): O70470 Q9WV46 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 26, 2002 |
Last sequence update: | July 26, 2002 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families