UniProtKB - Q9QX73 (ARHG9_RAT)
Protein
Rho guanine nucleotide exchange factor 9
Gene
Arhgef9
Organism
Rattus norvegicus (Rat)
Status
Functioni
Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters.3 Publications
GO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
GO - Biological processi
- receptor clustering Source: RGD
- regulation of postsynaptic specialization assembly Source: RGD
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Enzyme and pathway databases
Reactomei | R-RNO-193648, NRAGE signals death through JNK R-RNO-194840, Rho GTPase cycle R-RNO-416482, G alpha (12/13) signalling events R-RNO-977443, GABA receptor activation |
Names & Taxonomyi
Protein namesi | Recommended name: Rho guanine nucleotide exchange factor 9Alternative name(s): Collybistin Rac/Cdc42 guanine nucleotide exchange factor 9 |
Gene namesi | Name:Arhgef9 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620719, Arhgef9 |
Subcellular locationi
Other locations
- Cytoplasm 3 Publications
- postsynaptic density By similarity
Plasma Membrane
- plasma membrane Source: GOC
Other locations
- cell cortex Source: RGD
- GABA-ergic synapse Source: SynGO
- glycinergic synapse Source: SynGO
- postsynaptic density Source: UniProtKB
- postsynaptic specialization Source: SynGO
Keywords - Cellular componenti
Cell junction, Cytoplasm, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 107 | R → A: No effect on formation of GPHN clusters; when associated with A-108 and A-117. 2 Publications | 1 | |
Mutagenesisi | 108 | D → A: No effect on GPHN clusters; when associated with A-107 and A-117. 2 Publications | 1 | |
Mutagenesisi | 111 | R → A: Loss of formation of GPHN clusters. 2 Publications | 1 | |
Mutagenesisi | 117 | E → A: No effect on GPHN clusters; when associated with A-107 and A-108. 2 Publications | 1 | |
Mutagenesisi | 297 | R → H: Defects in binding to phosphatidylinositol-3-phosphate and in formation of GPHN clusters. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000253898 | 1 – 493 | Rho guanine nucleotide exchange factor 9Add BLAST | 493 |
Proteomic databases
PaxDbi | Q9QX73 |
PRIDEi | Q9QX73 |
PTM databases
PhosphoSitePlusi | Q9QX73 |
Expressioni
Tissue specificityi
Detected in brain, throughout the gray matter. Detected at low levels in heart and skeletal muscle.1 Publication
Interactioni
Subunit structurei
Interacts with GPHN.
4 PublicationsGO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB-KW
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9QX73 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9QX73 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 15 – 74 | SH3PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 110 – 294 | DHPROSITE-ProRule annotationAdd BLAST | 185 | |
Domaini | 325 – 432 | PHPROSITE-ProRule annotationAdd BLAST | 108 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 107 – 117 | Interaction with GPHNAdd BLAST | 11 |
Keywords - Domaini
SH3 domainPhylogenomic databases
InParanoidi | Q9QX73 |
OrthoDBi | 428887at2759 |
PhylomeDBi | Q9QX73 |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit cd11975, SH3_ARHGEF9, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
IDEALi | IID50103 |
InterProi | View protein in InterPro IPR035728, ARHGEF9_SH3 IPR035899, DBL_dom_sf IPR000219, DH-domain IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketNote: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9QX73-1) [UniParc]FASTAAdd to basket
Also known as: Collybistin I
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MQWIRGGSGM LITGDSIVSA EAVWDHVTMA NRGVAFKAGD VIKVLDASNK
60 70 80 90 100
DWWWGQIDDE EGWFPASFVR LWVNQEDGVE EGPSDVQNGH LDPNSDCLCL
110 120 130 140 150
GRPLQNRDQM RANVINEIMS TERHYIKHLK DICEGYLKQC RKRRDMFSDE
160 170 180 190 200
QLKVIFGNIE DIYRFQMGFV RDLEKQYNND DPHLSEIGPC FLEHQDGFWI
210 220 230 240 250
YSEYCNNHLD ACMELSKLMK DSRYQHFFEA CRLLQQMIDI AIDGFLLTPV
260 270 280 290 300
QKICKYPLQL AELLKYTAQD HSDYRYVAAA LAVMRNVTQQ INERKRRLEN
310 320 330 340 350
IDKIAQWQAS VLDWEGDDIL DRSSELIYTG EMAWIYQPYG RNQQRVFFLF
360 370 380 390 400
DHQMVLCKKD LIRRDILYYK GRIDMDKYEV IDIEDGRDDD FNVSMKNAFK
410 420 430 440 450
LHNKETEEVH LFFAKKLEEK IRWLRAFREE RKMVQEDEKI GFEISENQKR
460 470 480 490
QAAMTVRKAS KQKGRVGEEE NQSLELKRAC EVLQRLWSPG KKS
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_021147 | 12 – 71 | Missing in isoform 2. 1 PublicationAdd BLAST | 60 | |
Alternative sequenceiVSP_021148 | 464 – 493 | GRVGE…PGKKS → VTQRKWHY in isoform 2. 1 PublicationAdd BLAST | 30 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ250425 mRNA Translation: CAB65966.1 AJ302676 mRNA Translation: CAC16410.1 |
RefSeqi | NP_076447.1, NM_023957.1 [Q9QX73-1] |
Genome annotation databases
GeneIDi | 66013 |
KEGGi | rno:66013 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ250425 mRNA Translation: CAB65966.1 AJ302676 mRNA Translation: CAC16410.1 |
RefSeqi | NP_076447.1, NM_023957.1 [Q9QX73-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DFK | X-ray | 2.15 | A/C | 71-463 | [»] | |
4MT6 | X-ray | 5.50 | A | 1-456 | [»] | |
4MT7 | X-ray | 3.50 | A | 10-493 | [»] | |
SMRi | Q9QX73 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
PTM databases
PhosphoSitePlusi | Q9QX73 |
Proteomic databases
PaxDbi | Q9QX73 |
PRIDEi | Q9QX73 |
Protocols and materials databases
ABCDi | Q9QX73, 2 sequenced antibodies |
Genome annotation databases
GeneIDi | 66013 |
KEGGi | rno:66013 |
Organism-specific databases
CTDi | 23229 |
RGDi | 620719, Arhgef9 |
Phylogenomic databases
InParanoidi | Q9QX73 |
OrthoDBi | 428887at2759 |
PhylomeDBi | Q9QX73 |
Enzyme and pathway databases
Reactomei | R-RNO-193648, NRAGE signals death through JNK R-RNO-194840, Rho GTPase cycle R-RNO-416482, G alpha (12/13) signalling events R-RNO-977443, GABA receptor activation |
Miscellaneous databases
EvolutionaryTracei | Q9QX73 |
PROi | PR:Q9QX73 |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit cd11975, SH3_ARHGEF9, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
IDEALi | IID50103 |
InterProi | View protein in InterPro IPR035728, ARHGEF9_SH3 IPR035899, DBL_dom_sf IPR000219, DH-domain IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR036028, SH3-like_dom_sf IPR001452, SH3_domain |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF00621, RhoGEF, 1 hit PF00018, SH3_1, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit SM00326, SH3, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit SSF50044, SSF50044, 1 hit |
PROSITEi | View protein in PROSITE PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit PS50002, SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARHG9_RAT | |
Accessioni | Q9QX73Primary (citable) accession number: Q9QX73 Secondary accession number(s): Q9ER22 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references