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Protein

Zinc finger protein neuro-d4

Gene

Dpf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May have an important role in developing neurons by participating in regulation of cell survival, possibly as a neurospecific transcription factor. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri195 – 218C2H2-typePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri271 – 328PHD-type 1PROSITE-ProRule annotationAdd BLAST58
Zinc fingeri325 – 375PHD-type 2PROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

  • histone acetyltransferase activity Source: GO_Central
  • histone binding Source: GO_Central
  • nucleic acid binding Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Biological processNeurogenesis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein neuro-d4
Alternative name(s):
BRG1-associated factor 45B
Short name:
BAF45B
D4, zinc and double PHD fingers family 1
Gene namesi
Name:Dpf1
Synonyms:Baf45b, Neud4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1352748 Dpf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001681461 – 387Zinc finger protein neuro-d4Add BLAST387

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki106Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki129Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki133Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9QX66
PRIDEiQ9QX66

PTM databases

iPTMnetiQ9QX66
PhosphoSitePlusiQ9QX66

Expressioni

Tissue specificityi

At embryonic stages, predominant expression in the nervous system. Expressed specifically in post-mitotic neurons (at protein level).1 Publication

Developmental stagei

Low levels at embryonic day 9.5, and then sharp increase since embryonic day 12. In the developing forebrain and cerebellar primordium, strictly expressed in post-mitotic neurons.1 Publication

Gene expression databases

BgeeiENSMUSG00000030584 Expressed in 157 organ(s), highest expression level in brain
CleanExiMM_DPF1
ExpressionAtlasiQ9QX66 baseline and differential
GenevisibleiQ9QX66 MM

Interactioni

Subunit structurei

Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-1256 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant
CPX-1257 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant
CPX-1258 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant
CPX-1259 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant
STRINGi10090.ENSMUSP00000054385

Structurei

3D structure databases

ProteinModelPortaliQ9QX66
SMRiQ9QX66
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the requiem/DPF family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri195 – 218C2H2-typePROSITE-ProRule annotationAdd BLAST24
Zinc fingeri271 – 328PHD-type 1PROSITE-ProRule annotationAdd BLAST58
Zinc fingeri325 – 375PHD-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1244 Eukaryota
ENOG410YDAG LUCA
GeneTreeiENSGT00930000150839
HOGENOMiHOG000217918
HOVERGENiHBG004475
InParanoidiQ9QX66
KOiK22198
TreeFamiTF318971

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR038046 DPF1
IPR025750 Requiem/DPF_N_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10615:SF12 PTHR10615:SF12, 1 hit
PfamiView protein in Pfam
PF00628 PHD, 1 hit
PF14051 Requiem_N, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SM00355 ZnF_C2H2, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
SSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 2 hits
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Additional isoforms seem to exist.

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9QX66-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATVIPSPLS LGEDFYREAI EHCRSYNARL CAERSLRLPF LDSQTGVAQN
60 70 80 90 100
NCYIWMEKTH RGPGLAPGQI YTYPARCWRK KRRLNILEDP RLRPCEYKID
110 120 130 140 150
CEAPLKKEGG LPEGPVLEAL LCAETGEKKV ELKEEETIMD CQKQQLLEFP
160 170 180 190 200
HDLEVEDLEE DIPRRKNRAR GKAYGIGGLR KRQDTASLED RDKPYVCDIC
210 220 230 240 250
GKRYKNRPGL SYHYTHTHLA EEEGEEHTER HALPFHRKNN HKQFYKELAW
260 270 280 290 300
VPEAQRKHTA KKAPDGTVIP NGYCDFCLGG SKKTGCPEDL ISCADCGRSG
310 320 330 340 350
HPSCLQFTVN MTAAVRTYRW QCIECKSCSL CGTSENDDQL LFCDDCDRGY
360 370 380
HMYCLSPPMA EPPEGSWSCH LCLRHLKEKA SAYITLT
Length:387
Mass (Da):44,239
Last modified:April 27, 2001 - v2
Checksum:i9FC2F75EFB38CFF4
GO
Isoform 2 (identifier: Q9QX66-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MATVIPSPL → MATAIQNPLK

Show »
Length:388
Mass (Da):44,397
Checksum:iA729CA1367E12347
GO
Isoform 3 (identifier: Q9QX66-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.

Show »
Length:332
Mass (Da):37,950
Checksum:iE3E66134B170A99A
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YY44D3YY44_MOUSE
Zinc finger protein neuro-d4
Dpf1
398Annotation score:
F6YKQ9F6YKQ9_MOUSE
Zinc finger protein neuro-d4
Dpf1
146Annotation score:
F6UJZ6F6UJZ6_MOUSE
Zinc finger protein neuro-d4
Dpf1
143Annotation score:
D3YYG6D3YYG6_MOUSE
Zinc finger protein neuro-d4
Dpf1
353Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti314A → P. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0056091 – 55Missing in isoform 3. 1 PublicationAdd BLAST55
Alternative sequenceiVSP_0056081 – 9MATVIPSPL → MATAIQNPLK in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48238 mRNA Translation: AAF21455.1
U48239 mRNA Translation: AAF21456.1
AF108133 Genomic DNA Translation: AAF21305.1
CCDSiCCDS21071.1 [Q9QX66-2]
RefSeqiNP_038902.1, NM_013874.2 [Q9QX66-2]
UniGeneiMm.101885
Mm.453136
Mm.453137

Genome annotation databases

EnsembliENSMUST00000049977; ENSMUSP00000054385; ENSMUSG00000030584 [Q9QX66-2]
ENSMUST00000065181; ENSMUSP00000070539; ENSMUSG00000030584 [Q9QX66-1]
GeneIDi29861
KEGGimmu:29861

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48238 mRNA Translation: AAF21455.1
U48239 mRNA Translation: AAF21456.1
AF108133 Genomic DNA Translation: AAF21305.1
CCDSiCCDS21071.1 [Q9QX66-2]
RefSeqiNP_038902.1, NM_013874.2 [Q9QX66-2]
UniGeneiMm.101885
Mm.453136
Mm.453137

3D structure databases

ProteinModelPortaliQ9QX66
SMRiQ9QX66
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-1256 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant
CPX-1257 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant
CPX-1258 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant
CPX-1259 Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant
STRINGi10090.ENSMUSP00000054385

PTM databases

iPTMnetiQ9QX66
PhosphoSitePlusiQ9QX66

Proteomic databases

PaxDbiQ9QX66
PRIDEiQ9QX66

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049977; ENSMUSP00000054385; ENSMUSG00000030584 [Q9QX66-2]
ENSMUST00000065181; ENSMUSP00000070539; ENSMUSG00000030584 [Q9QX66-1]
GeneIDi29861
KEGGimmu:29861

Organism-specific databases

CTDi8193
MGIiMGI:1352748 Dpf1

Phylogenomic databases

eggNOGiKOG1244 Eukaryota
ENOG410YDAG LUCA
GeneTreeiENSGT00930000150839
HOGENOMiHOG000217918
HOVERGENiHBG004475
InParanoidiQ9QX66
KOiK22198
TreeFamiTF318971

Miscellaneous databases

PROiPR:Q9QX66
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030584 Expressed in 157 organ(s), highest expression level in brain
CleanExiMM_DPF1
ExpressionAtlasiQ9QX66 baseline and differential
GenevisibleiQ9QX66 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR038046 DPF1
IPR025750 Requiem/DPF_N_dom
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10615:SF12 PTHR10615:SF12, 1 hit
PfamiView protein in Pfam
PF00628 PHD, 1 hit
PF14051 Requiem_N, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SM00355 ZnF_C2H2, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
SSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 2 hits
PS00028 ZINC_FINGER_C2H2_1, 1 hit
PS50157 ZINC_FINGER_C2H2_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPF1_MOUSE
AccessioniPrimary (citable) accession number: Q9QX66
Secondary accession number(s): Q9QX65, Q9QYA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: November 7, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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