UniProtKB - Q9QX11 (CYH1_MOUSE)
Cytohesin-1
Cyth1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 281 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 | |
Binding sitei | 292 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 | |
Binding sitei | 302 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 | |
Binding sitei | 351 | Phosphatidylinositol 3,4,5-trisphosphateBy similarity | 1 |
GO - Molecular functioni
- ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
- lipid binding Source: UniProtKB-KW
GO - Biological processi
- establishment of epithelial cell polarity Source: MGI
- regulation of ARF protein signal transduction Source: InterPro
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Ligand | Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-6811438, Intra-Golgi traffic |
Names & Taxonomyi
Protein namesi | Recommended name: Cytohesin-1Alternative name(s): PH, SEC7 and coiled-coil domain-containing protein 1 Short name: CLM1 SEC7 homolog A Short name: mSec7-1 |
Gene namesi | Name:Cyth1 Synonyms:Pscd1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1334257, Cyth1 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Peripheral membrane protein 1 Publication
Cytosol
- cytosol 2 Publications
Other locations
- tight junction 1 Publication
- adherens junction 1 Publication
Note: Colocalized with TJP1 during epithelial polarization (PubMed:20080746).1 Publication
Cytosol
- cytosol Source: UniProtKB
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- adherens junction Source: UniProtKB-SubCell
- bicellular tight junction Source: MGI
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Membrane, Tight junctionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 157 | E → K: Impairs epithelium polarity. 1 Publication | 1 | |
Mutagenesisi | 385 | L → A: Impairs autoinhibition; when associated with A-389. Impairs translocation to the cell membrane. 1 Publication | 1 | |
Mutagenesisi | 389 | K → A: Impairs autoinhibition; when associated with A-385. Impairs translocation to the cell membrane. 1 Publication | 1 | |
Mutagenesisi | 394 | S → E: Increases guanine exchange factor activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120195 | 1 – 398 | Cytohesin-1Add BLAST | 398 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
PaxDbi | Q9QX11 |
PRIDEi | Q9QX11 |
PTM databases
iPTMneti | Q9QX11 |
PhosphoSitePlusi | Q9QX11 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000017132, Expressed in bone marrow and 281 other tissues |
ExpressionAtlasi | Q9QX11, baseline and differential |
Genevisiblei | Q9QX11, MM |
Interactioni
Subunit structurei
Interacts with TRIM23 and CYTIP (By similarity).
Interacts (via coiled-coil domain) with FRMD4A (via coiled-coil domain)(PubMed:20080746).
Interacts with FRMD4B (PubMed:20080746).
Found in a complex with PARD3, CYTH1 and FRMD4A (PubMed:20080746).
Interacts (via N-terminal domain) with INAVA (via N-terminal domain) (By similarity).
By similarity1 PublicationGO - Molecular functioni
- ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 202411, 4 interactors |
IntActi | Q9QX11, 2 interactors |
STRINGi | 10090.ENSMUSP00000017276 |
Miscellaneous databases
RNActi | Q9QX11, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 73 – 202 | SEC7PROSITE-ProRule annotationAdd BLAST | 130 | |
Domaini | 260 – 377 | PHPROSITE-ProRule annotationAdd BLAST | 118 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 60 | Necessary for localization at adherens junction1 PublicationAdd BLAST | 60 | |
Regioni | 269 – 277 | Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity | 9 | |
Regioni | 388 – 396 | C-terminal autoinhibitory regionBy similarity | 9 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 10 – 67 | Sequence analysisAdd BLAST | 58 |
Domaini
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0930, Eukaryota |
GeneTreei | ENSGT00940000157519 |
HOGENOMi | CLU_032820_3_0_1 |
InParanoidi | Q9QX11 |
OMAi | GGRYKNW |
TreeFami | TF352091 |
Family and domain databases
CDDi | cd00171, Sec7, 1 hit |
Gene3Di | 1.10.1000.11, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR023394, Sec7_C_sf IPR000904, Sec7_dom IPR035999, Sec7_dom_sf |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF01369, Sec7, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00222, Sec7, 1 hit |
SUPFAMi | SSF48425, SSF48425, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50190, SEC7, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEDDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKEE IAEVANEIES
60 70 80 90 100
LGSTEERKNM QRNKQVAMGR KKFNMDPKKG IQFLIENGLL KNTCEDIAQF
110 120 130 140 150
LYKGEGLNKT AIGDYLGERD EFSIQVLHAF VELHEFTDLN LVQALRQFLW
160 170 180 190 200
SFRLPGEAQK IDRMMEAFAQ RYCQCNTGVF QSTDTCYVLS FAIIMLNTSL
210 220 230 240 250
HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK NEPFKIPEDD
260 270 280 290 300
GNDLTHTFFN PDREGWLLKL GGGRVKTWKR RWFILTDNCL YYFEYTTDKE
310 320 330 340 350
PRGIIPLENL SIREVEDSKK PNCFELYIPD NKDQVIKACK TEADGRVVEG
360 370 380 390
NHTVYRISAP TPEEKEDWIK CIKAAISRDP FYEMLAARKK KVSSTKRH
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3TZ02 | Q3TZ02_MOUSE | Cytohesin-1 | Cyth1 Pscd1 | 400 | Annotation score: | ||
B1AQE4 | B1AQE4_MOUSE | Cytohesin-1 | Cyth1 | 99 | Annotation score: | ||
A2A517 | A2A517_MOUSE | Cytohesin-1 | Cyth1 | 460 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | D → E in AAC71694 (PubMed:9745014).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_006035 | 273 | Missing in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB013464 mRNA Translation: BAA33428.1 AF051337 mRNA Translation: AAC71694.1 AB035538 mRNA Translation: BAB13509.1 |
CCDSi | CCDS48997.1 [Q9QX11-2] |
RefSeqi | NP_001106169.1, NM_001112699.1 [Q9QX11-2] NP_035310.2, NM_011180.3 [Q9QX11-1] |
Genome annotation databases
Ensembli | ENSMUST00000017276; ENSMUSP00000017276; ENSMUSG00000017132 [Q9QX11-2] ENSMUST00000106305; ENSMUSP00000101912; ENSMUSG00000017132 [Q9QX11-1] |
GeneIDi | 19157 |
KEGGi | mmu:19157 |
UCSCi | uc011yil.1, mouse [Q9QX11-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB013464 mRNA Translation: BAA33428.1 AF051337 mRNA Translation: AAC71694.1 AB035538 mRNA Translation: BAB13509.1 |
CCDSi | CCDS48997.1 [Q9QX11-2] |
RefSeqi | NP_001106169.1, NM_001112699.1 [Q9QX11-2] NP_035310.2, NM_011180.3 [Q9QX11-1] |
3D structure databases
SMRi | Q9QX11 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202411, 4 interactors |
IntActi | Q9QX11, 2 interactors |
STRINGi | 10090.ENSMUSP00000017276 |
PTM databases
iPTMneti | Q9QX11 |
PhosphoSitePlusi | Q9QX11 |
Proteomic databases
PaxDbi | Q9QX11 |
PRIDEi | Q9QX11 |
Protocols and materials databases
Antibodypediai | 19745, 248 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000017276; ENSMUSP00000017276; ENSMUSG00000017132 [Q9QX11-2] ENSMUST00000106305; ENSMUSP00000101912; ENSMUSG00000017132 [Q9QX11-1] |
GeneIDi | 19157 |
KEGGi | mmu:19157 |
UCSCi | uc011yil.1, mouse [Q9QX11-1] |
Organism-specific databases
CTDi | 9267 |
MGIi | MGI:1334257, Cyth1 |
Phylogenomic databases
eggNOGi | KOG0930, Eukaryota |
GeneTreei | ENSGT00940000157519 |
HOGENOMi | CLU_032820_3_0_1 |
InParanoidi | Q9QX11 |
OMAi | GGRYKNW |
TreeFami | TF352091 |
Enzyme and pathway databases
Reactomei | R-MMU-6811438, Intra-Golgi traffic |
Miscellaneous databases
BioGRID-ORCSi | 19157, 1 hit in 17 CRISPR screens |
ChiTaRSi | Cyth1, mouse |
PROi | PR:Q9QX11 |
RNActi | Q9QX11, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000017132, Expressed in bone marrow and 281 other tissues |
ExpressionAtlasi | Q9QX11, baseline and differential |
Genevisiblei | Q9QX11, MM |
Family and domain databases
CDDi | cd00171, Sec7, 1 hit |
Gene3Di | 1.10.1000.11, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR011993, PH-like_dom_sf IPR001849, PH_domain IPR023394, Sec7_C_sf IPR000904, Sec7_dom IPR035999, Sec7_dom_sf |
Pfami | View protein in Pfam PF00169, PH, 1 hit PF01369, Sec7, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00222, Sec7, 1 hit |
SUPFAMi | SSF48425, SSF48425, 1 hit |
PROSITEi | View protein in PROSITE PS50003, PH_DOMAIN, 1 hit PS50190, SEC7, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYH1_MOUSE | |
Accessioni | Q9QX11Primary (citable) accession number: Q9QX11 Secondary accession number(s): O88817, Q76MU4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 10, 2021 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot