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Protein

Kinesin-like protein KIFC1

Gene

Kifc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Minus end-directed microtubule-dependent motor required for bipolar spindle formation (PubMed:16638812). May contribute to movement of early endocytic vesicles (PubMed:17360972). Regulates cilium formation and structure (PubMed:23807208).3 Publications

Miscellaneous

Purified early endocytic vesicles bind minus end-directed Kifc1 as well as plus end-directed Kif5b. Addition of anti-Kifc1 antibodies leads to a decrease in minus end-directed vesicle motility in vitro.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi411 – 418ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, minus-end-directed Source: MGI
  • microtubule binding Source: InterPro
  • microtubule motor activity Source: MGI

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • minus-end-directed vesicle transport along microtubule Source: MGI
  • mitotic metaphase plate congression Source: UniProtKB
  • mitotic spindle assembly Source: UniProtKB
  • negative regulation of centrosome duplication Source: MGI
  • vesicle transport along microtubule Source: MGI

Keywordsi

Molecular functionMotor protein
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIFC1
Gene namesi
Name:Kifc1Imported
Synonyms:Kifc41 Publication, Kifc5a1 Publication, Kifc5b1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:109596 Kifc1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Endosome, Microtubule, Nucleus

Pathology & Biotechi

Disruption phenotypei

Centrosome amplification as well as multipolar spindles. Cells overexpressing Kifc1 show a single microtubule aster and growth arrest in prometaphase.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003020891 – 674Kinesin-like protein KIFC1Add BLAST674

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineBy similarity1
Modified residuei33PhosphoserineBy similarity1
Modified residuei35PhosphoserineBy similarity1
Modified residuei360PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9QWT9
PaxDbiQ9QWT9
PeptideAtlasiQ9QWT9
PRIDEiQ9QWT9

PTM databases

iPTMnetiQ9QWT9
PhosphoSitePlusiQ9QWT9

Expressioni

Tissue specificityi

Highly expressed in E14 embryos, spleen and NIH3T3 cells. Also expressed in testis, brain, lung, kidney and cultured astrocytes. Very low levels in skeletal muscle and heart.2 Publications

Developmental stagei

Highly expressed in hippocampus of E13 embryos declining to low levels by E18 and to undetectable levels in juvenile and adult hippocampus.1 Publication

Gene expression databases

BgeeiENSMUSG00000079553
CleanExiMM_KIFC1
ExpressionAtlasiQ9QWT9 baseline and differential
GenevisibleiQ9QWT9 MM

Interactioni

Subunit structurei

Binds NUBP1 and NUBP2 (PubMed:16638812). Interacts with PPP1R42 (PubMed:18237440).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200952, 8 interactors
224976, 49 interactors
IntActiQ9QWT9, 9 interactors
STRINGi10090.ENSMUSP00000134572

Structurei

3D structure databases

ProteinModelPortaliQ9QWT9
SMRiQ9QWT9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini311 – 664Kinesin motorPROSITE-ProRule annotationAdd BLAST354

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili146 – 315Sequence analysisAdd BLAST170

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. NCD subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0239 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00920000149025
HOVERGENiHBG001080
InParanoidiQ9QWT9
KOiK10405
OMAiPVMSSKR
OrthoDBiEOG091G0DJX
PhylomeDBiQ9QWT9
TreeFamiTF105237

Family and domain databases

Gene3Di3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q9QWT9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDVQAQRPPL LEVKRNVELK AALVKSSSRV PLSASRLKRG PDQMEDALEP
60 70 80 90 100
AKKRTRVMGA VTKVDTSRPR GPLLSTVSQT QGHTAAQKGP KKTGPRGCSA
110 120 130 140 150
IGTVLRSQKP VPAAPAQKPG TSTAPVVVGK RAGKRPAWDL KGQLCDLNEE
160 170 180 190 200
LKRYREKTQT LELENRGLRE QLREVQEQAT TLGTERNTLE GELASVRSRA
210 220 230 240 250
EQDQQRLETL SARVLELEEC LGTRERLLQE LQGERLQLQE ERSTLSTQLE
260 270 280 290 300
EQERRFQATE AALSSSQEEV VCLRQKTEAQ VTLLAEQGDR LYGLEMERRR
310 320 330 340 350
LHNQLQELKG NIRVFCRVRP VLEGESTPSP GFLVFPPGPA GPSDPPTGLS
360 370 380 390 400
LSRSDDRRST LTGAPAPTVR HDFSFDRVFP PGSKQEEVFE EIAMLVQSAL
410 420 430 440 450
DGYPVCIFAY GQTGSGKTFT MEGGPRGDPQ LEGLIPRAMR HLFSVAQEMS
460 470 480 490 500
GQGWTYSFVA SYVEIYNETV RDLLATGPRK GQGGECEIRR ASPGSEELTV
510 520 530 540 550
TNARYVPVSC EKEVEALLHL AHQNRAVAHT AQNKRSSRSH SVFQLQISGE
560 570 580 590 600
HAARGLQCGA PLNLVDLAGS ERLDPGLHLG PGERDRLRET QAINSSLSTL
610 620 630 640 650
GLVIMALSNK ESHVPYRNSK LTYLLQNSLG GSAKMLMFVN ISPLEENVSE
660 670
SLNSLRFASK VNQCVIGTAQ ANKK
Length:674
Mass (Da):74,153
Last modified:September 11, 2007 - v2
Checksum:iA99B6FB6F9BC1B03
GO
Isoform 21 Publication (identifier: Q9QWT9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Show »
Length:631
Mass (Da):69,401
Checksum:iE74F26745611BCB4
GO
Isoform 31 Publication (identifier: Q9QWT9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.
     203-222: Missing.

Show »
Length:611
Mass (Da):67,117
Checksum:iBF5B0CA4DE84A5C3
GO

Sequence cautioni

The sequence BAA19676 differs from that shown. Reason: Frameshift at positions 223 and 244.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101I → V in BAA19676 (PubMed:9115736).Curated1
Sequence conflicti101I → V in AAF34646 (PubMed:10775188).Curated1
Sequence conflicti101I → V in CAJ19646 (PubMed:16638812).Curated1
Sequence conflicti101I → V in AAH03753 (PubMed:15489334).Curated1
Sequence conflicti101I → V in AAH57162 (PubMed:15489334).Curated1
Sequence conflicti101I → V in AAI00329 (PubMed:15489334).Curated1
Sequence conflicti111V → A in BAA19676 (PubMed:9115736).Curated1
Sequence conflicti111V → A in AAF34646 (PubMed:10775188).Curated1
Sequence conflicti111V → A in CAJ19646 (PubMed:16638812).Curated1
Sequence conflicti111V → A in AAH03753 (PubMed:15489334).Curated1
Sequence conflicti111V → A in AAH57162 (PubMed:15489334).Curated1
Sequence conflicti111V → A in AAI00329 (PubMed:15489334).Curated1
Sequence conflicti160T → M in BAA19676 (PubMed:9115736).Curated1
Sequence conflicti160T → M in AAF34646 (PubMed:10775188).Curated1
Sequence conflicti160T → M in CAJ19646 (PubMed:16638812).Curated1
Sequence conflicti253E → K in BAA19676 (PubMed:9115736).Curated1
Sequence conflicti253E → K in AAF34646 (PubMed:10775188).Curated1
Sequence conflicti253E → K in CAJ19646 (PubMed:16638812).Curated1
Sequence conflicti253E → K in AAH03753 (PubMed:15489334).Curated1
Sequence conflicti271V → L in AAH57162 (PubMed:15489334).Curated1
Sequence conflicti315F → L in CAJ19646 (PubMed:16638812).Curated1
Sequence conflicti323E → A in BAA19676 (PubMed:9115736).Curated1
Sequence conflicti323E → A in AAF34646 (PubMed:10775188).Curated1
Sequence conflicti323E → A in CAJ19646 (PubMed:16638812).Curated1
Sequence conflicti323E → A in AAH03753 (PubMed:15489334).Curated1
Sequence conflicti323E → A in AAI00329 (PubMed:15489334).Curated1
Sequence conflicti345P → L in AAI00329 (PubMed:15489334).Curated1
Sequence conflicti432E → A in BAC38230 (PubMed:16141072).Curated1
Sequence conflicti478P → L in BAA19676 (PubMed:9115736).Curated1
Sequence conflicti503A → V in BAA19676 (PubMed:9115736).Curated1
Sequence conflicti566D → G in AAF34646 (PubMed:10775188).Curated1
Sequence conflicti578H → P in BAC38230 (PubMed:16141072).Curated1
Sequence conflicti619S → R in BAA19676 (PubMed:9115736).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0525261 – 43Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST43
Alternative sequenceiVSP_052527203 – 222Missing in isoform 3. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49544 mRNA Translation: BAA19676.1 Frameshift.
AF221102 mRNA Translation: AAF34646.1
AM051187 mRNA Translation: CAJ19646.1
AF110520 Genomic DNA Translation: AAC97970.1
BC003753 mRNA Translation: AAH03753.1
BC057162 mRNA Translation: AAH57162.1
BC100328 mRNA Translation: AAI00329.1
AK081484 mRNA Translation: BAC38230.1
AF013117 mRNA Translation: AAC39966.1
CCDSiCCDS57065.1 [Q9QWT9-1]
RefSeqiNP_001182227.1, NM_001195298.1 [Q9QWT9-1]
NP_444403.2, NM_053173.2
UniGeneiMm.335713
Mm.491138

Genome annotation databases

EnsembliENSMUST00000114361; ENSMUSP00000110001; ENSMUSG00000079553 [Q9QWT9-3]
ENSMUST00000173492; ENSMUSP00000134572; ENSMUSG00000079553 [Q9QWT9-1]
GeneIDi100502766
16580
KEGGimmu:100502766
mmu:16580
UCSCiuc008bzw.2 mouse [Q9QWT9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKIFC1_MOUSE
AccessioniPrimary (citable) accession number: Q9QWT9
Secondary accession number(s): O08671
, O35230, Q497Y3, Q4A1N2, Q6PG90, Q8BV06, Q99L84, Q9JKZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: July 18, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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