Cd5l

Functionⁱ

Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflammed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis (PubMed:26048980). Able to inhibit lipid droplet size in adipocytes (PubMed:20519120, PubMed:22579686). Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA) (PubMed:20519120). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease (PubMed:21730133). Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC) (PubMed:22579686, PubMed:26607793). Acts as a key regulator of metabolic switch in T-helper Th17 cells (PubMed:26607794, PubMed:26607793). Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation (PubMed:26607793). CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes (PubMed:26607793). Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies (PubMed:23562157). Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis (PubMed:9892623, PubMed:16054063). Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy (By similarity).1 PublicationBy similarity8 Publications

GO - Molecular functionⁱ

scavenger receptor activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic process Source: UniProtKB-KW
immune system process Source: UniProtKB-KW
inflammatory response Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Apoptosis, Immunity, Inflammatory response

Biological process

Apoptosis, Immunity, Inflammatory response

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: CD5 antigen-like Alternative name(s): Apoptosis inhibitor expressed by macrophages1 Publication Short name: mAIM1 Publication Apoptosis inhibitory 6 SP-alpha1 Publication
Gene namesⁱ	Name:Cd5l Synonyms:Aim1 Publication, Api6
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 3

Organism-specific databases

MGIⁱ	MGI:1334419 Cd5l

Keywords - Cellular componentⁱ

Cytoplasm, Secreted

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Mice are apparently healthy under specific pathogen-free conditions. However, thymus of mice display much fewer thymocytes and CD4/CD8 double-positive (DP) thymocytes are more susceptible to apoptosis (PubMed:9892623). Increased adipocyte size and adipose tissue mass (PubMed:20519120). Higher level of free cholesterol in Th17 cells (PubMed:26607793).3 Publications

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	99	N → Q: Decreased glycosylation. 1 Publication	1
Mutagenesisⁱ	229	N → Q: Decreased glycosylation. 1 Publication	1
Mutagenesisⁱ	316	N → Q: Does not affect glycosylation. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 21	Sequence analysisAdd BLAST		21
ChainⁱPRO_0000033226	22 – 352	CD5 antigen-likeAdd BLAST		331

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	36 ↔ 70	PROSITE-ProRule annotation
Disulfide bondⁱ	52 ↔ 117	PROSITE-ProRule annotation
Disulfide bondⁱ	65 ↔ 127	PROSITE-ProRule annotation
Disulfide bondⁱ	98 ↔ 108	PROSITE-ProRule annotation
Glycosylationⁱ	99	N-linked (GlcNAc...) asparagine1 Publication	1
Disulfide bondⁱ	166 ↔ 230	PROSITE-ProRule annotation
Disulfide bondⁱ	179 ↔ 240	PROSITE-ProRule annotation
Disulfide bondⁱ	211 ↔ 221	PROSITE-ProRule annotation
Glycosylationⁱ	229	N-linked (GlcNAc...) asparagine1 Publication	1
Disulfide bondⁱ	255 ↔ 289	PROSITE-ProRule annotation
Disulfide bondⁱ	271 ↔ 337	PROSITE-ProRule annotation
Disulfide bondⁱ	284 ↔ 347	PROSITE-ProRule annotation
Disulfide bondⁱ	317 ↔ 327	PROSITE-ProRule annotation

Post-translational modificationⁱ

N-glycosylated (PubMed:10651944, PubMed:23236605). N-glycan at Asn-99 possesses only alpha2,6-sialylated terminals, while Asn-229 possesses both alpha2,6-sialylated and non-sialylated terminals (PubMed:23236605). N-glycosylation increases secretion.2 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	316	Not glycosylated1 Publication		1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9QWK4
PaxDbⁱ	Q9QWK4
PeptideAtlas More...PeptideAtlasⁱ	Q9QWK4
PRIDEⁱ	Q9QWK4

PTM databases

iPTMnetⁱ	Q9QWK4
PhosphoSitePlusⁱ	Q9QWK4

Expressionⁱ

Tissue specificityⁱ

Specifically expressed in tissue macrophages (PubMed:9892623). Expressed in thymus, liver, spleen and lymph nodes (PubMed:10651944). Present in Th17 cells; mainly present in non-pathogenic Th17 cells (PubMed:26607793).3 Publications

Inductionⁱ

Transcription is activated by nuclear receptor liver X /retinoid X (RXR/LXR).

Gene expression databases

Bgeeⁱ	ENSMUSG00000015854 Expressed in 75 organ(s), highest expression level in liver
CleanExⁱ	MM_CD5L
Genevisibleⁱ	Q9QWK4 MM

Interactionⁱ

Subunit structureⁱ

Interacts with FASN; the interaction is direct (PubMed:20519120). Interacts with IgM; protecting CD5L from renal excretion and leading to increased CD5L levels in circulating blood (PubMed:23562157).2 Publications

Protein-protein interaction databases

BioGridⁱ	198152, 1 interactor
IntActⁱ	Q9QWK4, 1 interactor
Molecular INTeraction database More...MINTⁱ	Q9QWK4
STRINGⁱ	10090.ENSMUSP00000015998

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9QWK4
SMRⁱ	Q9QWK4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	27 – 128	SRCR 1PROSITE-ProRule annotationAdd BLAST	102
Domainⁱ	141 – 241	SRCR 2PROSITE-ProRule annotationAdd BLAST	101
Domainⁱ	246 – 348	SRCR 3PROSITE-ProRule annotationAdd BLAST	103

Keywords - Domainⁱ

Repeat, Signal

Phylogenomic databases

eggNOGⁱ	ENOG410IKHN Eukaryota ENOG4110209 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140803
HOGENOMⁱ	HOG000290652
HOVERGENⁱ	HBG031373
InParanoidⁱ	Q9QWK4
OMAⁱ	YHGEDAS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0DF7
TreeFamⁱ	TF329295

Family and domain databases

Gene3Dⁱ	3.10.250.10, 3 hits
InterProⁱ	View protein in InterPro IPR001190 SRCR IPR017448 SRCR-like_dom IPR036772 SRCR-like_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00530 SRCR, 3 hits
PRINTSⁱ	PR00258 SPERACTRCPTR
SMARTⁱ	View protein in SMART SM00202 SR, 3 hits
SUPFAMⁱ	SSF56487 SSF56487, 3 hits
PROSITEⁱ	View protein in PROSITE PS00420 SRCR_1, 1 hit PS50287 SRCR_2, 3 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9QWK4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAPLFNLMLA ILSIFVGSCF SESPTKVQLV GGAHRCEGRV EVEHNGQWGT 
        60         70         80         90        100
VCDDGWDRRD VAVVCRELNC GAVIQTPRGA SYQPPASEQR VLIQGVDCNG 
       110        120        130        140        150
TEDTLAQCEL NYDVFDCSHE EDAGAQCENP DSDLLFIPED VRLVDGPGHC 
       160        170        180        190        200
QGRVEVLHQS QWSTVCKAGW NLQVSKVVCR QLGCGRALLT YGSCNKNTQG 
       210        220        230        240        250
KGPIWMGKMS CSGQEANLRS CLLSRLENNC THGEDTWMEC EDPFELKLVG 
       260        270        280        290        300
GDTPCSGRLE VLHKGSWGSV CDDNWGEKED QVVCKQLGCG KSLHPSPKTR 
       310        320        330        340        350
KIYGPGAGRI WLDDVNCSGK EQSLEFCRHR LWGYHDCTHK EDVEVICTDF 

DV

Length:352

Mass (Da):38,863

Last modified:July 27, 2011 - v3

Checksum:ⁱ41596AA8012E1AEE

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	13	S → N in AAB70571 (PubMed:10651944).Curated		1
Sequence conflictⁱ	113	D → Y in AAD01445 (PubMed:9892623).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	61	V → M1 Publication	1
Natural variantⁱ	197	N → S2 Publications	1
Natural variantⁱ	205	W → R1 Publication	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF018268 mRNA Translation: AAB70571.1 AF018269 mRNA Translation: AAB70572.1 AF011428 mRNA Translation: AAD01445.1 AK159132 mRNA Translation: BAE34844.1 AK159181 mRNA Translation: BAE34880.1 AK159823 mRNA Translation: BAE35403.1 BC006799 mRNA Translation: AAH06799.1 BC094459 mRNA Translation: AAH94459.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS17451.1
NCBI Reference Sequences More...RefSeqⁱ	NP_033820.2, NM_009690.2
UniGeneⁱ	Mm.6676

Genome annotation databases

Ensemblⁱ	ENSMUST00000015998; ENSMUSP00000015998; ENSMUSG00000015854
GeneIDⁱ	11801
KEGGⁱ	mmu:11801
UCSC genome browser More...UCSCⁱ	uc008psa.2 mouse

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9QWK4	CD5 antigen-like		DIPOR		338	UniRef50_Q9QWK4
CD5 antigen-like isoform X2		MESAU		337
CD5 molecule like		LOXAF		354
Uncharacterized protein		SARHA		341
Uncharacterized protein		MONDO		339
+16

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF018268 mRNA Translation: AAB70571.1 AF018269 mRNA Translation: AAB70572.1 AF011428 mRNA Translation: AAD01445.1 AK159132 mRNA Translation: BAE34844.1 AK159181 mRNA Translation: BAE34880.1 AK159823 mRNA Translation: BAE35403.1 BC006799 mRNA Translation: AAH06799.1 BC094459 mRNA Translation: AAH94459.1
CCDSⁱ	CCDS17451.1
RefSeqⁱ	NP_033820.2, NM_009690.2
UniGeneⁱ	Mm.6676

3D structure databases

ProteinModelPortalⁱ	Q9QWK4
SMRⁱ	Q9QWK4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	198152, 1 interactor
IntActⁱ	Q9QWK4, 1 interactor
MINTⁱ	Q9QWK4
STRINGⁱ	10090.ENSMUSP00000015998

PTM databases

iPTMnetⁱ	Q9QWK4
PhosphoSitePlusⁱ	Q9QWK4

Proteomic databases

MaxQBⁱ	Q9QWK4
PaxDbⁱ	Q9QWK4
PeptideAtlasⁱ	Q9QWK4
PRIDEⁱ	Q9QWK4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000015998; ENSMUSP00000015998; ENSMUSG00000015854
GeneIDⁱ	11801
KEGGⁱ	mmu:11801
UCSCⁱ	uc008psa.2 mouse

Organism-specific databases

CTDⁱ	922
MGIⁱ	MGI:1334419 Cd5l

Phylogenomic databases

eggNOGⁱ	ENOG410IKHN Eukaryota ENOG4110209 LUCA
GeneTreeⁱ	ENSGT00900000140803
HOGENOMⁱ	HOG000290652
HOVERGENⁱ	HBG031373
InParanoidⁱ	Q9QWK4
OMAⁱ	YHGEDAS
OrthoDBⁱ	EOG091G0DF7
TreeFamⁱ	TF329295

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9QWK4
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000015854 Expressed in 75 organ(s), highest expression level in liver
CleanExⁱ	MM_CD5L
Genevisibleⁱ	Q9QWK4 MM

Family and domain databases

Gene3Dⁱ	3.10.250.10, 3 hits
InterProⁱ	View protein in InterPro IPR001190 SRCR IPR017448 SRCR-like_dom IPR036772 SRCR-like_dom_sf
Pfamⁱ	View protein in Pfam PF00530 SRCR, 3 hits
PRINTSⁱ	PR00258 SPERACTRCPTR
SMARTⁱ	View protein in SMART SM00202 SR, 3 hits
SUPFAMⁱ	SSF56487 SSF56487, 3 hits
PROSITEⁱ	View protein in PROSITE PS00420 SRCR_1, 1 hit PS50287 SRCR_2, 3 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CD5L_MOUSE
Accessionⁱ	Q9QWK4Primary (citable) accession number: Q9QWK4 Secondary accession number(s): O35300 , O35301, Q3TXN5, Q505P6, Q91W05
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
	Last sequence update:	July 27, 2011
	Last modified:	October 10, 2018
	This is version 135 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9QWK4 (CD5L_MOUSE)

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CD5 antigen-like

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Other locations

Extracellular region or secreted

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ