UniProtKB - Q9QWJ9 (NRP1_RAT)
Neuropilin-1
Nrp1
Functioni
Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (By similarity).
Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).
Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 195 | CalciumBy similarity | 1 | |
Metal bindingi | 209 | CalciumBy similarity | 1 | |
Metal bindingi | 250 | CalciumBy similarity | 1 |
GO - Molecular functioni
- growth factor binding Source: RGD
- GTPase activator activity Source: RGD
- heparin binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein kinase binding Source: RGD
- semaphorin receptor activity Source: RGD
- vascular endothelial growth factor-activated receptor activity Source: RGD
- vascular endothelial growth factor binding Source: RGD
GO - Biological processi
- actin cytoskeleton reorganization Source: RGD
- angiogenesis Source: RGD
- angiogenesis involved in coronary vascular morphogenesis Source: RGD
- artery morphogenesis Source: RGD
- axonal fasciculation Source: RGD
- axon extension involved in axon guidance Source: RGD
- axon guidance Source: RGD
- axonogenesis involved in innervation Source: RGD
- basal dendrite arborization Source: RGD
- basal dendrite development Source: RGD
- branching involved in blood vessel morphogenesis Source: RGD
- branchiomotor neuron axon guidance Source: RGD
- cell migration Source: RGD
- cell migration involved in sprouting angiogenesis Source: RGD
- cellular response to hepatocyte growth factor stimulus Source: RGD
- cellular response to vascular endothelial growth factor stimulus Source: RGD
- commissural neuron axon guidance Source: RGD
- coronary artery morphogenesis Source: RGD
- dendrite development Source: RGD
- dichotomous subdivision of terminal units involved in salivary gland branching Source: RGD
- dorsal root ganglion morphogenesis Source: RGD
- endothelial cell chemotaxis Source: RGD
- endothelial cell migration Source: RGD
- facial nerve structural organization Source: RGD
- facioacoustic ganglion development Source: RGD
- gonadotrophin-releasing hormone neuronal migration to the hypothalamus Source: RGD
- heart development Source: RGD
- hepatocyte growth factor receptor signaling pathway Source: RGD
- integrin-mediated signaling pathway Source: RGD
- motor neuron axon guidance Source: RGD
- motor neuron migration Source: UniProtKB
- negative regulation of axon extension Source: RGD
- negative regulation of axon extension involved in axon guidance Source: RGD
- negative regulation of extrinsic apoptotic signaling pathway Source: RGD
- negative regulation of neuron apoptotic process Source: RGD
- nerve development Source: RGD
- nervous system development Source: RGD
- neural crest cell migration involved in autonomic nervous system development Source: RGD
- neuron development Source: RGD
- neuron migration Source: RGD
- neuropilin signaling pathway Source: RGD
- otic placode development Source: RGD
- outflow tract septum morphogenesis Source: RGD
- platelet-derived growth factor receptor signaling pathway Source: RGD
- positive chemotaxis Source: RGD
- positive regulation of actin cytoskeleton reorganization Source: RGD
- positive regulation of angiogenesis Source: RGD
- positive regulation of axon extension involved in axon guidance Source: RGD
- positive regulation of cell migration involved in sprouting angiogenesis Source: RGD
- positive regulation of endothelial cell migration Source: RGD
- positive regulation of ERK1 and ERK2 cascade Source: RGD
- positive regulation of filopodium assembly Source: RGD
- positive regulation of focal adhesion assembly Source: RGD
- positive regulation of peptidyl-tyrosine phosphorylation Source: RGD
- positive regulation of phosphorylation Source: RGD
- positive regulation of retinal ganglion cell axon guidance Source: RGD
- positive regulation of stress fiber assembly Source: RGD
- positive regulation of substrate adhesion-dependent cell spreading Source: RGD
- postsynapse organization Source: RGD
- protein localization to early endosome Source: RGD
- regulation of axon extension involved in axon guidance Source: RGD
- regulation of Cdc42 protein signal transduction Source: RGD
- regulation of retinal ganglion cell axon guidance Source: RGD
- renal artery morphogenesis Source: RGD
- response to wounding Source: RGD
- retinal ganglion cell axon guidance Source: RGD
- retina vasculature morphogenesis in camera-type eye Source: RGD
- semaphorin-plexin signaling pathway Source: RGD
- semaphorin-plexin signaling pathway involved in axon guidance Source: RGD
- semaphorin-plexin signaling pathway involved in neuron projection guidance Source: RGD
- sensory neuron axon guidance Source: RGD
- sprouting angiogenesis Source: RGD
- substrate adhesion-dependent cell spreading Source: RGD
- substrate-dependent cell migration, cell extension Source: RGD
- sympathetic ganglion development Source: RGD
- sympathetic nervous system development Source: RGD
- sympathetic neuron projection extension Source: RGD
- sympathetic neuron projection guidance Source: RGD
- toxin transport Source: RGD
- trigeminal ganglion development Source: RGD
- trigeminal nerve morphogenesis Source: RGD
- trigeminal nerve structural organization Source: RGD
- vascular endothelial growth factor receptor signaling pathway Source: RGD
- VEGF-activated neuropilin signaling pathway Source: RGD
- VEGF-activated neuropilin signaling pathway involved in axon guidance Source: RGD
- ventral trunk neural crest cell migration Source: RGD
- vestibulocochlear nerve structural organization Source: RGD
- viral entry into host cell Source: RGD
Keywordsi
Molecular function | Developmental protein, Heparin-binding, Receptor |
Biological process | Angiogenesis, Differentiation, Neurogenesis |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-194306, Neurophilin interactions with VEGF and VEGFR R-RNO-399954, Sema3A PAK dependent Axon repulsion R-RNO-399955, SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion R-RNO-399956, CRMPs in Sema3A signaling R-RNO-445144, Signal transduction by L1 |
Names & Taxonomyi
Protein namesi | Recommended name: Neuropilin-1Alternative name(s): Vascular endothelial cell growth factor 165 receptor CD_antigen: CD304 |
Gene namesi | Name:Nrp1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621588, Nrp1 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Mitochondrion
- Mitochondrion membrane By similarity; Single-pass type I membrane protein Sequence analysis
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Cytoskeleton
- neurofilament Source: RGD
Cytosol
- cytosol Source: RGD
Endosome
- early endosome Source: RGD
- sorting endosome Source: RGD
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
Plasma Membrane
- integral component of postsynaptic membrane Source: RGD
- plasma membrane Source: RGD
Other locations
- axon Source: RGD
- cell surface Source: RGD
- focal adhesion Source: RGD
- glutamatergic synapse Source: RGD
- growth cone Source: RGD
- integral component of membrane Source: RGD
- neuron projection Source: RGD
- neuronal cell body Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 22 – 855 | ExtracellularSequence analysisAdd BLAST | 834 | |
Transmembranei | 856 – 880 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 881 – 922 | CytoplasmicSequence analysisAdd BLAST | 42 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
ChainiPRO_0000021861 | 22 – 922 | Neuropilin-1Add BLAST | 901 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 27 ↔ 54 | 1 Publication | ||
Disulfide bondi | 82 ↔ 104 | 1 Publication | ||
Disulfide bondi | 147 ↔ 173 | By similarity | ||
Glycosylationi | 150 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 206 ↔ 228 | By similarity | ||
Glycosylationi | 261 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 275 ↔ 424 | 1 Publication | ||
Glycosylationi | 300 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 431 ↔ 583 | 1 Publication | ||
Glycosylationi | 522 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 612 | O-linked (Xyl...) (chondroitin sulfate) serine; alternateBy similarity | 1 | |
Glycosylationi | 612 | O-linked (Xyl...) (heparan sulfate) serine; alternateBy similarity | 1 | |
Glycosylationi | 841 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 893 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Heparan sulfate, Phosphoprotein, ProteoglycanProteomic databases
PaxDbi | Q9QWJ9 |
PRIDEi | Q9QWJ9 |
PTM databases
GlyGeni | Q9QWJ9, 6 sites |
iPTMneti | Q9QWJ9 |
PhosphoSitePlusi | Q9QWJ9 |
SwissPalmi | Q9QWJ9 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSRNOG00000010744, Expressed in lung and 20 other tissues |
Genevisiblei | Q9QWJ9, RN |
Interactioni
Subunit structurei
Homodimer, and heterodimer with NRP2. Binds PLXNB1 (By similarity).
Interacts with FER.
Interacts with VEGFA (By similarity).
Interacts with ABCB8/MITOSUR in mitochondria (By similarity).
By similarityGO - Molecular functioni
- growth factor binding Source: RGD
- protein kinase binding Source: RGD
- vascular endothelial growth factor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 251596, 4 interactors |
DIPi | DIP-44935N |
IntActi | Q9QWJ9, 2 interactors |
STRINGi | 10116.ENSRNOP00000014492 |
Chemistry databases
BindingDBi | Q9QWJ9 |
Structurei
Secondary structure
3D structure databases
SMRi | Q9QWJ9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9QWJ9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 141 | CUB 1PROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 147 – 265 | CUB 2PROSITE-ProRule annotationAdd BLAST | 119 | |
Domaini | 275 – 424 | F5/8 type C 1PROSITE-ProRule annotationAdd BLAST | 150 | |
Domaini | 431 – 583 | F5/8 type C 2PROSITE-ProRule annotationAdd BLAST | 153 | |
Domaini | 645 – 811 | MAMPROSITE-ProRule annotationAdd BLAST | 167 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QUEH, Eukaryota |
GeneTreei | ENSGT00940000157169 |
HOGENOMi | CLU_015228_6_1_1 |
InParanoidi | Q9QWJ9 |
OMAi | HPYTNEW |
OrthoDBi | 124611at2759 |
PhylomeDBi | Q9QWJ9 |
TreeFami | TF316506 |
Family and domain databases
CDDi | cd00041, CUB, 2 hits cd00057, FA58C, 2 hits cd06263, MAM, 1 hit |
Gene3Di | 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR013320, ConA-like_dom_sf IPR000859, CUB_dom IPR000421, FA58C IPR008979, Galactose-bd-like_sf IPR000998, MAM_dom IPR014648, Neuropilin IPR022579, Neuropilin_C IPR027146, NRP1 IPR035914, Sperma_CUB_dom_sf |
PANTHERi | PTHR46806:SF4, PTHR46806:SF4, 1 hit |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF11980, DUF3481, 1 hit PF00754, F5_F8_type_C, 2 hits PF00629, MAM, 1 hit |
PIRSFi | PIRSF036960, Neuropilin, 1 hit |
PRINTSi | PR00020, MAMDOMAIN |
SMARTi | View protein in SMART SM00042, CUB, 2 hits SM00231, FA58C, 2 hits SM00137, MAM, 1 hit |
SUPFAMi | SSF49785, SSF49785, 2 hits SSF49854, SSF49854, 2 hits SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS01180, CUB, 2 hits PS01285, FA58C_1, 2 hits PS01286, FA58C_2, 2 hits PS50022, FA58C_3, 2 hits PS00740, MAM_1, 1 hit PS50060, MAM_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MERGLPLLCA TLALALALAG AFRSDKCGGT IKIENPGYLT SPGYPHSYHP
60 70 80 90 100
SEKCEWLIQA PEPYQRIMIN FNPHFDLEDR DCKYDYVEVI DGENEGGRLW
110 120 130 140 150
GKFCGKIAPS PVVSSGPFLF IKFVSDYETH GAGFSIRYEI FKRGPECSQN
160 170 180 190 200
YTAPTGVIKS PGFPEKYPNS LECTYIIFAP KMSEIILEFE SFDLEQDSNP
210 220 230 240 250
PGGVFCRYDR LEIWDGFPEV GPHIGRYCGQ KTPGRIRSSS GILSMVFYTD
260 270 280 290 300
SAIAKEGFSA NYSVLQSSIS EDFKCMEALG MESGEIHSDQ ITASSQYGTN
310 320 330 340 350
WSVERSRLNY PENGWTPGED SYREWIQVDL GLLRFVTAVG TQGAISKETK
360 370 380 390 400
KKYYVKTYRV DISSNGEDWI TLKEGNKAII FQGNTNPTDV VFGVFPKPLI
410 420 430 440 450
TRFVRIKPAS WETGISMRFE VYGCKITDYP CSGMLGMVSG LISDSQITAS
460 470 480 490 500
NQGDRNWMPE NIRLVTSRTG WALPPSPHPY INEWLQVDLG DEKIVRGVII
510 520 530 540 550
QGGKHRENKV FMRKFKIAYS NNGSDWKMIM DDSKRKAKSF EGNNNYDTPE
560 570 580 590 600
LRAFTPLSTR FIRIYPERAT HSGLGLRMEL LGCEVEVPTA GPTTPNGNPV
610 620 630 640 650
DECDDDQANC HSGTGDDFQL TGGTTVLATE KPTIIDSTIQ SEFPTYGFNC
660 670 680 690 700
EFGWGSHKTF CHWEHDSHAQ LRWRVLTSKT GPIQDHTGDG NFIYSQADEN
710 720 730 740 750
QKGKVARLVS PVVYSQSSAH CMTFWYHMSG SHVGTLRVKL HYQKPEEYDQ
760 770 780 790 800
LVWMVVGHQG DHWKEGRVLL HKSLKLYQVI FEGEIGKGNL GGIAVDDISI
810 820 830 840 850
NNHIPQEDCA KPTDLDKKNT EIKIDETGST PGYEEGKGDK NISRKPGNVL
860 870 880 890 900
KTLDPILITI IAMSALGVLL GAVCGVVLYC ACWHNGMSER NLSALENYNF
910 920
ELVDGVKLKK DKLNPQSNYS EA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF016296 mRNA Translation: AAC53337.1 BC085689 mRNA Translation: AAH85689.1 |
RefSeqi | NP_659566.1, NM_145098.2 XP_006255887.1, XM_006255825.3 |
Genome annotation databases
Ensembli | ENSRNOT00000014492; ENSRNOP00000014492; ENSRNOG00000010744 ENSRNOT00000080465; ENSRNOP00000075034; ENSRNOG00000010744 |
GeneIDi | 246331 |
KEGGi | rno:246331 |
UCSCi | RGD:621588, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF016296 mRNA Translation: AAC53337.1 BC085689 mRNA Translation: AAH85689.1 |
RefSeqi | NP_659566.1, NM_145098.2 XP_006255887.1, XM_006255825.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ORX | X-ray | 2.40 | A | 273-586 | [»] | |
2ORZ | X-ray | 2.15 | A | 273-586 | [»] | |
SMRi | Q9QWJ9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 251596, 4 interactors |
DIPi | DIP-44935N |
IntActi | Q9QWJ9, 2 interactors |
STRINGi | 10116.ENSRNOP00000014492 |
Chemistry databases
BindingDBi | Q9QWJ9 |
ChEMBLi | CHEMBL3309098 |
PTM databases
GlyGeni | Q9QWJ9, 6 sites |
iPTMneti | Q9QWJ9 |
PhosphoSitePlusi | Q9QWJ9 |
SwissPalmi | Q9QWJ9 |
Proteomic databases
PaxDbi | Q9QWJ9 |
PRIDEi | Q9QWJ9 |
Genome annotation databases
Ensembli | ENSRNOT00000014492; ENSRNOP00000014492; ENSRNOG00000010744 ENSRNOT00000080465; ENSRNOP00000075034; ENSRNOG00000010744 |
GeneIDi | 246331 |
KEGGi | rno:246331 |
UCSCi | RGD:621588, rat |
Organism-specific databases
CTDi | 8829 |
RGDi | 621588, Nrp1 |
Phylogenomic databases
eggNOGi | ENOG502QUEH, Eukaryota |
GeneTreei | ENSGT00940000157169 |
HOGENOMi | CLU_015228_6_1_1 |
InParanoidi | Q9QWJ9 |
OMAi | HPYTNEW |
OrthoDBi | 124611at2759 |
PhylomeDBi | Q9QWJ9 |
TreeFami | TF316506 |
Enzyme and pathway databases
Reactomei | R-RNO-194306, Neurophilin interactions with VEGF and VEGFR R-RNO-399954, Sema3A PAK dependent Axon repulsion R-RNO-399955, SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion R-RNO-399956, CRMPs in Sema3A signaling R-RNO-445144, Signal transduction by L1 |
Miscellaneous databases
EvolutionaryTracei | Q9QWJ9 |
PROi | PR:Q9QWJ9 |
Gene expression databases
Bgeei | ENSRNOG00000010744, Expressed in lung and 20 other tissues |
Genevisiblei | Q9QWJ9, RN |
Family and domain databases
CDDi | cd00041, CUB, 2 hits cd00057, FA58C, 2 hits cd06263, MAM, 1 hit |
Gene3Di | 2.60.120.290, 2 hits |
InterProi | View protein in InterPro IPR013320, ConA-like_dom_sf IPR000859, CUB_dom IPR000421, FA58C IPR008979, Galactose-bd-like_sf IPR000998, MAM_dom IPR014648, Neuropilin IPR022579, Neuropilin_C IPR027146, NRP1 IPR035914, Sperma_CUB_dom_sf |
PANTHERi | PTHR46806:SF4, PTHR46806:SF4, 1 hit |
Pfami | View protein in Pfam PF00431, CUB, 2 hits PF11980, DUF3481, 1 hit PF00754, F5_F8_type_C, 2 hits PF00629, MAM, 1 hit |
PIRSFi | PIRSF036960, Neuropilin, 1 hit |
PRINTSi | PR00020, MAMDOMAIN |
SMARTi | View protein in SMART SM00042, CUB, 2 hits SM00231, FA58C, 2 hits SM00137, MAM, 1 hit |
SUPFAMi | SSF49785, SSF49785, 2 hits SSF49854, SSF49854, 2 hits SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS01180, CUB, 2 hits PS01285, FA58C_1, 2 hits PS01286, FA58C_2, 2 hits PS50022, FA58C_3, 2 hits PS00740, MAM_1, 1 hit PS50060, MAM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NRP1_RAT | |
Accessioni | Q9QWJ9Primary (citable) accession number: Q9QWJ9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | May 1, 2000 | |
Last modified: | September 29, 2021 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families