UniProtKB - Q9QWH1 (PHC2_MOUSE)
Polyhomeotic-like protein 2
Phc2
Functioni
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 634 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 637 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 653 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 657 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 625 – 659 | FCS-typePROSITE-ProRule annotationAdd BLAST | 35 |
GO - Molecular functioni
- chromatin binding Source: GO_Central
- DNA binding Source: UniProtKB-KW
- histone binding Source: GO_Central
- identical protein binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: GO_Central
- spermatogenesis Source: MGI
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-2559580, Oxidative Stress Induced Senescence R-MMU-3108214, SUMOylation of DNA damage response and repair proteins R-MMU-3899300, SUMOylation of transcription cofactors R-MMU-4551638, SUMOylation of chromatin organization proteins R-MMU-4570464, SUMOylation of RNA binding proteins R-MMU-8939243, RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known R-MMU-8943724, Regulation of PTEN gene transcription |
Names & Taxonomyi
Protein namesi | Recommended name: Polyhomeotic-like protein 2Short name: mPH2 Alternative name(s): Early development regulatory protein 2 p36 |
Gene namesi | Name:Phc2 Synonyms:Edr2, Ph2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1860454, Phc2 |
VEuPathDBi | HostDB:ENSMUSG00000028796 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
- PcG protein complex Source: MGI
- PRC1 complex Source: MGI
Other locations
- heterochromatin Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076287 | 1 – 850 | Polyhomeotic-like protein 2Add BLAST | 850 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 590 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 592 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 611 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 613 | PhosphoserineBy similarity | 1 | |
Cross-linki | 624 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 694 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 743 | PhosphoserineBy similarity | 1 | |
Cross-linki | 839 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9QWH1 |
MaxQBi | Q9QWH1 |
PaxDbi | Q9QWH1 |
PeptideAtlasi | Q9QWH1 |
PRIDEi | Q9QWH1 |
ProteomicsDBi | 289489 [Q9QWH1-1] 289490 [Q9QWH1-2] |
PTM databases
iPTMneti | Q9QWH1 |
PhosphoSitePlusi | Q9QWH1 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000028796, Expressed in neocortex and 322 other tissues |
ExpressionAtlasi | Q9QWH1, baseline and differential |
Genevisiblei | Q9QWH1, MM |
Interactioni
Subunit structurei
Component of a PRC1-like complex.
Interacts with CBX4 (By similarity).
Interacts with BMI1, PCGF2, PHC1 and RNF2 (PubMed:9627119, PubMed:16024804, PubMed:27827373).
Interacts with CHTOP (PubMed:22872859).
Interacts with the N-terminal region of the SP1 transcription factor and with MAPKAPK2 (By similarity).
By similarity4 PublicationsBinary interactionsi
Q9QWH1
GO - Molecular functioni
- histone binding Source: GO_Central
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207636, 16 interactors |
IntActi | Q9QWH1, 8 interactors |
MINTi | Q9QWH1 |
STRINGi | 10090.ENSMUSP00000101690 |
Miscellaneous databases
RNActi | Q9QWH1, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9QWH1 |
SMRi | Q9QWH1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 786 – 850 | SAMPROSITE-ProRule annotationAdd BLAST | 65 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 79 | Disordered1 PublicationAdd BLAST | 79 | |
Regioni | 33 – 56 | Interaction with BMI11 PublicationAdd BLAST | 24 | |
Regioni | 233 – 314 | DisorderedSequence analysisAdd BLAST | 82 | |
Regioni | 335 – 386 | DisorderedSequence analysisAdd BLAST | 52 | |
Regioni | 402 – 436 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 528 – 553 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 597 – 624 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 676 – 712 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 725 – 764 | DisorderedSequence analysisAdd BLAST | 40 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 550 – 579 | HD1Add BLAST | 30 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 38 | Polar residuesSequence analysisAdd BLAST | 38 | |
Compositional biasi | 338 – 362 | Pro residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 363 – 379 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 528 – 549 | Polar residuesSequence analysisAdd BLAST | 22 | |
Compositional biasi | 610 – 624 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 689 – 712 | Polar residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 725 – 754 | Polar residuesSequence analysisAdd BLAST | 30 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 625 – 659 | FCS-typePROSITE-ProRule annotationAdd BLAST | 35 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QS5Q, Eukaryota |
GeneTreei | ENSGT00940000160840 |
HOGENOMi | CLU_012048_0_0_1 |
InParanoidi | Q9QWH1 |
OMAi | PTPNQVQ |
OrthoDBi | 298184at2759 |
PhylomeDBi | Q9QWH1 |
TreeFami | TF331299 |
Family and domain databases
DisProti | DP01315 |
Gene3Di | 1.10.150.50, 1 hit 3.30.60.160, 1 hit |
InterProi | View protein in InterPro IPR001660, SAM IPR013761, SAM/pointed_sf IPR012313, Znf_FCS IPR038603, Znf_FCS_sf |
Pfami | View protein in Pfam PF00536, SAM_1, 1 hit |
SMARTi | View protein in SMART SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS50105, SAM_DOMAIN, 1 hit PS51024, ZF_FCS, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MENELPVPHT SNRASVTTNT SGTNSSSGCI SSSGGGGGSG GRPTAPQISV
60 70 80 90 100
YSGIPDRQTV QVIQQALHRQ PSTAAQYLQQ MYAAQQQHLM LQTAALQQQH
110 120 130 140 150
LSSAQLQSLA AVQQASLVAN RQGSTPGSSV SSQAPAQSSS LNLAASPAAA
160 170 180 190 200
QLINRAQSVN SAAASGLAQQ AVLLGNTSSP ALTASQAQMY LRAQMLIFTP
210 220 230 240 250
TATVATVQPE LCTGSPARPP TPAQVQNLTL RTQQTPAAAA SGPPPTQPVL
260 270 280 290 300
PSLALKPTPS SSQPLPAPPQ GRTMAQGSPA GAKPSGTDNA PETLKAGDGN
310 320 330 340 350
CNMEGRPGPG RAVPAVATHP LIAPAYAHLQ SHQLLPQPPA KHPQPQFVAQ
360 370 380 390 400
QQPQPPRPAP QVQSQPQLAS VSPSLALQSS PEDHALPLGS VTQALPLQCS
410 420 430 440 450
TTHVHKPGNS QQCHLPTLDT GSQNGHPEGG SHPPQRRFQH TSAVILQVQP
460 470 480 490 500
ASPVTPQQCA PDDWKEVVPA EKSVPVARPG PSPHQQAIIP AIPGGLPGPK
510 520 530 540 550
SPNIQQCPAH ETGQGIVHAL TDLSSPGMTS GNGNSASSIA GTAPQNGENK
560 570 580 590 600
PPQAIVKPQI LTHVIEGFVI QEGAEPFPVG RSSLLVGNLK KKYAQGFLPE
610 620 630 640 650
KPPQQDHTTT TDSEMEEPYL QESKEEGTPL KLKCELCGRV DFAYKFKRSK
660 670 680 690 700
RFCSMACAKR YNVGCTKRVG LFHSDRSKLQ KAGTTTHNRR RASKASLPTL
710 720 730 740 750
TKDTKKQPSG TVPLSVTAAL QLAHSQEDSS RCSDNSSYEE PLSPISASSS
760 770 780 790 800
TSRRRQGQRD LDLPDMHMRD LVGVGHHFLP SEPTKWNVED VYEFIRSLPG
810 820 830 840 850
CQEIAEEFRA QEIDGQALLL LKEDHLMSAM NIKLGPALKI YARISMLKDS
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB1ASA3 | B1ASA3_MOUSE | Polyhomeotic-like 2 (Drosophila) | Phc2 RP23-467J23.1-008 | 202 | Annotation score: | ||
B1ASA2 | B1ASA2_MOUSE | Polyhomeotic-like 2 (Drosophila) | Phc2 RP23-467J23.1-009 | 143 | Annotation score: | ||
B1AS97 | B1AS97_MOUSE | Polyhomeotic-like 2 (Drosophila) | Phc2 RP23-467J23.1-002 | 269 | Annotation score: | ||
B1ASA1 | B1ASA1_MOUSE | Polyhomeotic-like protein 2 | Phc2 | 53 | Annotation score: | ||
D6REH8 | D6REH8_MOUSE | Polyhomeotic-like protein 2 | Phc2 | 40 | Annotation score: | ||
B1ASA0 | B1ASA0_MOUSE | Polyhomeotic-like 2 (Drosophila) | Phc2 RP23-467J23.1-011 | 22 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016919 | 1 – 527 | Missing in isoform 2. 2 PublicationsAdd BLAST | 527 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB062362 mRNA Translation: BAB93527.1 U81491 mRNA Translation: AAD00519.1 AL611969 Genomic DNA No translation available. AL611983 Genomic DNA No translation available. BC057571 mRNA Translation: AAH57571.1 BC071246 mRNA Translation: AAH71246.1 AF060076 mRNA Translation: AAC23570.1 |
CCDSi | CCDS18674.1 [Q9QWH1-1] CCDS57297.1 [Q9QWH1-2] |
RefSeqi | NP_001182012.1, NM_001195083.1 [Q9QWH1-2] NP_001182059.1, NM_001195130.1 [Q9QWH1-1] NP_061244.1, NM_018774.4 [Q9QWH1-1] XP_006503300.1, XM_006503237.3 [Q9QWH1-2] XP_006503301.1, XM_006503238.1 [Q9QWH1-2] XP_017175809.1, XM_017320320.1 [Q9QWH1-1] |
Genome annotation databases
Ensembli | ENSMUST00000030588; ENSMUSP00000030588; ENSMUSG00000028796 [Q9QWH1-1] ENSMUST00000106079; ENSMUSP00000101689; ENSMUSG00000028796 [Q9QWH1-2] ENSMUST00000106080; ENSMUSP00000101690; ENSMUSG00000028796 [Q9QWH1-1] |
GeneIDi | 54383 |
KEGGi | mmu:54383 |
UCSCi | uc008uvh.2, mouse [Q9QWH1-1] uc008uvk.2, mouse [Q9QWH1-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB062362 mRNA Translation: BAB93527.1 U81491 mRNA Translation: AAD00519.1 AL611969 Genomic DNA No translation available. AL611983 Genomic DNA No translation available. BC057571 mRNA Translation: AAH57571.1 BC071246 mRNA Translation: AAH71246.1 AF060076 mRNA Translation: AAC23570.1 |
CCDSi | CCDS18674.1 [Q9QWH1-1] CCDS57297.1 [Q9QWH1-2] |
RefSeqi | NP_001182012.1, NM_001195083.1 [Q9QWH1-2] NP_001182059.1, NM_001195130.1 [Q9QWH1-1] NP_061244.1, NM_018774.4 [Q9QWH1-1] XP_006503300.1, XM_006503237.3 [Q9QWH1-2] XP_006503301.1, XM_006503238.1 [Q9QWH1-2] XP_017175809.1, XM_017320320.1 [Q9QWH1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2NA1 | NMR | - | A | 557-590 | [»] | |
AlphaFoldDBi | Q9QWH1 | |||||
SMRi | Q9QWH1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 207636, 16 interactors |
IntActi | Q9QWH1, 8 interactors |
MINTi | Q9QWH1 |
STRINGi | 10090.ENSMUSP00000101690 |
PTM databases
iPTMneti | Q9QWH1 |
PhosphoSitePlusi | Q9QWH1 |
Proteomic databases
EPDi | Q9QWH1 |
MaxQBi | Q9QWH1 |
PaxDbi | Q9QWH1 |
PeptideAtlasi | Q9QWH1 |
PRIDEi | Q9QWH1 |
ProteomicsDBi | 289489 [Q9QWH1-1] 289490 [Q9QWH1-2] |
Protocols and materials databases
Antibodypediai | 31416, 111 antibodies from 22 providers |
DNASUi | 54383 |
Genome annotation databases
Ensembli | ENSMUST00000030588; ENSMUSP00000030588; ENSMUSG00000028796 [Q9QWH1-1] ENSMUST00000106079; ENSMUSP00000101689; ENSMUSG00000028796 [Q9QWH1-2] ENSMUST00000106080; ENSMUSP00000101690; ENSMUSG00000028796 [Q9QWH1-1] |
GeneIDi | 54383 |
KEGGi | mmu:54383 |
UCSCi | uc008uvh.2, mouse [Q9QWH1-1] uc008uvk.2, mouse [Q9QWH1-2] |
Organism-specific databases
CTDi | 1912 |
MGIi | MGI:1860454, Phc2 |
VEuPathDBi | HostDB:ENSMUSG00000028796 |
Phylogenomic databases
eggNOGi | ENOG502QS5Q, Eukaryota |
GeneTreei | ENSGT00940000160840 |
HOGENOMi | CLU_012048_0_0_1 |
InParanoidi | Q9QWH1 |
OMAi | PTPNQVQ |
OrthoDBi | 298184at2759 |
PhylomeDBi | Q9QWH1 |
TreeFami | TF331299 |
Enzyme and pathway databases
Reactomei | R-MMU-2559580, Oxidative Stress Induced Senescence R-MMU-3108214, SUMOylation of DNA damage response and repair proteins R-MMU-3899300, SUMOylation of transcription cofactors R-MMU-4551638, SUMOylation of chromatin organization proteins R-MMU-4570464, SUMOylation of RNA binding proteins R-MMU-8939243, RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known R-MMU-8943724, Regulation of PTEN gene transcription |
Miscellaneous databases
BioGRID-ORCSi | 54383, 1 hit in 70 CRISPR screens |
ChiTaRSi | Phc2, mouse |
PROi | PR:Q9QWH1 |
RNActi | Q9QWH1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028796, Expressed in neocortex and 322 other tissues |
ExpressionAtlasi | Q9QWH1, baseline and differential |
Genevisiblei | Q9QWH1, MM |
Family and domain databases
DisProti | DP01315 |
Gene3Di | 1.10.150.50, 1 hit 3.30.60.160, 1 hit |
InterProi | View protein in InterPro IPR001660, SAM IPR013761, SAM/pointed_sf IPR012313, Znf_FCS IPR038603, Znf_FCS_sf |
Pfami | View protein in Pfam PF00536, SAM_1, 1 hit |
SMARTi | View protein in SMART SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit |
PROSITEi | View protein in PROSITE PS50105, SAM_DOMAIN, 1 hit PS51024, ZF_FCS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHC2_MOUSE | |
Accessioni | Q9QWH1Primary (citable) accession number: Q9QWH1 Secondary accession number(s): B1AS98 Q8K5D9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2006 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references