UniProtKB - Q9QWF0 (CAF1A_MOUSE)
Protein
Chromatin assembly factor 1 subunit A
Gene
Chaf1a
Organism
Mus musculus (Mouse)
Status
Functioni
Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.By similarity
GO - Molecular functioni
- chromo shadow domain binding Source: MGI
- identical protein binding Source: MGI
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- chromatin assembly Source: UniProtKB
- DNA repair Source: UniProtKB-KW
- DNA replication Source: UniProtKB-KW
- DNA replication-dependent nucleosome assembly Source: MGI
- nucleosome assembly Source: GO_Central
Keywordsi
Molecular function | Chaperone |
Biological process | Cell cycle, DNA damage, DNA repair, DNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: Chromatin assembly factor 1 subunit ACuratedShort name: CAF-1 subunit A Alternative name(s): Chromatin assembly factor I p150 subunit Short name: CAF-I 150 kDa subunit Short name: CAF-I p150 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351331, Chaf1a |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: DNA replication foci.By similarity
Nucleus
- nuclear chromatin Source: MGI
- nucleus Source: GO_Central
Other locations
- CAF-1 complex Source: UniProtKB
- protein-containing complex Source: MGI
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 224 | V → N or E: Prevents binding to CBX1 and CBX5. 1 Publication | 1 | |
Mutagenesisi | 224 | V → S or D: Prevents binding to CBX1 and CBX5. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000089277 | 1 – 911 | Chromatin assembly factor 1 subunit AAdd BLAST | 911 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 190 | PhosphoserineBy similarity | 1 | |
Modified residuei | 208 | PhosphoserineBy similarity | 1 | |
Modified residuei | 293 | PhosphoserineBy similarity | 1 | |
Modified residuei | 776 | PhosphoserineBy similarity | 1 | |
Modified residuei | 838 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9QWF0 |
jPOSTi | Q9QWF0 |
MaxQBi | Q9QWF0 |
PaxDbi | Q9QWF0 |
PeptideAtlasi | Q9QWF0 |
PRIDEi | Q9QWF0 |
PTM databases
iPTMneti | Q9QWF0 |
PhosphoSitePlusi | Q9QWF0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000002835, Expressed in primary oocyte and 82 other tissues |
ExpressionAtlasi | Q9QWF0, baseline and differential |
Interactioni
Subunit structurei
Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. CHAF1A binds directly to PCNA and to CBX1.
Interacts with MBD1.
Interacts directly with CBX5 via the PxVxL motif.
Interacts with CBX5.
By similarityBinary interactionsi
Hide detailsGO - Molecular functioni
- chromo shadow domain binding Source: MGI
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 205142, 163 interactors |
ComplexPortali | CPX-570, Chromatin assembly factor 1 complex |
ELMi | Q9QWF0 |
IntActi | Q9QWF0, 17 interactors |
STRINGi | 10090.ENSMUSP00000002914 |
Miscellaneous databases
RNActi | Q9QWF0, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9QWF0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9QWF0 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 31 | Binds PCNABy similarityAdd BLAST | 31 | |
Regioni | 176 – 327 | Binds CBX1 and CBX3 chromo shadow domainsAdd BLAST | 152 | |
Regioni | 621 – 657 | Necessary for homodimerization and competence for chromatin assemblyBy similarityAdd BLAST | 37 | |
Regioni | 639 – 911 | Binds to p60By similarityAdd BLAST | 273 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 217 – 230 | PxVxL motifAdd BLAST | 14 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 311 – 445 | Arg/Glu/Lys-richAdd BLAST | 135 | |
Compositional biasi | 546 – 641 | Asp/Glu-rich (acidic)Add BLAST | 96 | |
Compositional biasi | 582 – 587 | Poly-Glu | 6 |
Domaini
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
Sequence similaritiesi
Belongs to the CHAF1A family.Curated
Phylogenomic databases
eggNOGi | KOG4364, Eukaryota |
GeneTreei | ENSGT00440000034888 |
HOGENOMi | CLU_014846_0_0_1 |
InParanoidi | Q9QWF0 |
OMAi | CRKPRKT |
OrthoDBi | 1362011at2759 |
PhylomeDBi | Q9QWF0 |
TreeFami | TF350377 |
Family and domain databases
IDEALi | IID50294 |
InterProi | View protein in InterPro IPR029105, CAF1-p150_C2 IPR029091, CAF1_p150_N IPR022043, CAF1A |
Pfami | View protein in Pfam PF15539, CAF1-p150_C2, 1 hit PF15557, CAF1-p150_N, 1 hit PF12253, CAF1A, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9QWF0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLEEPEAATR TAAAVDCKDR PGFPVKRLIQ ARLPFKRLNL VPKEKVEEDT
60 70 80 90 100
SPKAAVESKV PDLQLSLGTF ESQCHTGSHV GLSTKLVGGQ GPIDSFLRAT
110 120 130 140 150
IKPVPSVVII DLTENCSDIP DSPEGHSELS PDTAGVVTTV EGAAKQQEHS
160 170 180 190 200
AAELCLLETP SDITCHMEEE PGSPGDPKRT GDCQAGSLQS CPELTPGSRT
210 220 230 240 250
CPTKELSSWS KAGDLLFIEK VPVVVLEDIL ATKPSIASLP MMSLDRSVTS
260 270 280 290 300
ESEILESCPE DDSILSHSST NSSSPTSSPE GPSTPPEHRG GRSSPSTPAC
310 320 330 340 350
RVAKNFVKGS TEKGRSKLHR DREQQREEKE KLREEIRRAK EEARKKKEEE
360 370 380 390 400
KELKEKERRE KREKDEKEKA EKQRLKEEKR KERQEALEAK LEEKRKKEEE
410 420 430 440 450
KRLREEEKRL REEEKRIKAE KAEITRFFQK PKTPQAPKTL AGSCGKFAPF
460 470 480 490 500
EIKEHMVLAP RCRAALDQDL CDQLDQLLQQ QSVASTFLSD LKSRLPLRSG
510 520 530 540 550
PTRVCGHDTD IMNRDVVIVE SSKVDGVSER KKFGRMKLLQ FSENHRPAYW
560 570 580 590 600
GTWNKKTAII RPRNPWAQDK DLLDYEVDSD DEWEEEEPGE SLSHSEGDED
610 620 630 640 650
DDVGEDEDED DGFFVPHGYL SEDEGVTEEC ADPENHKVHQ KLKAKEWDEL
660 670 680 690 700
LAKGKRFRVL QPVHVGCVWA SEAANCTSSD LKLLQQFTAC LLDVASPDEP
710 720 730 740 750
EPGASRREKR DQHILAQLLP LLHGNVNGSK VIIHEFQEQC RRGLLTLPSP
760 770 780 790 800
TPHLQMPNLE DAVAVPSKAR LKRLISENSA YEKRPNFRMC WYVHPEVLKS
810 820 830 840 850
FGQECLPVPC QWTYITTMPS APREDSGSAS TEGPGQSTPM LLKRKPAATM
860 870 880 890 900
CITQFMKKRR YDGQVGSGDM DGFQADTEED EEDDTDCMII DVPDVGSDVS
910
EAPIPAPTLC K
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A3B2WD19 | A0A3B2WD19_MOUSE | Chromatin assembly factor 1 subunit... | Chaf1a | 226 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ132771 mRNA Translation: CAB55497.2 AK034839 mRNA Translation: BAC28848.1 AK161016 mRNA Translation: BAE36149.1 CH466559 Genomic DNA Translation: EDL23708.1 BC053740 mRNA Translation: AAH53740.1 |
CCDSi | CCDS28892.1 |
RefSeqi | NP_038761.1, NM_013733.3 |
Genome annotation databases
Ensembli | ENSMUST00000002914; ENSMUSP00000002914; ENSMUSG00000002835 |
GeneIDi | 27221 |
KEGGi | mmu:27221 |
UCSCi | uc008dar.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ132771 mRNA Translation: CAB55497.2 AK034839 mRNA Translation: BAC28848.1 AK161016 mRNA Translation: BAE36149.1 CH466559 Genomic DNA Translation: EDL23708.1 BC053740 mRNA Translation: AAH53740.1 |
CCDSi | CCDS28892.1 |
RefSeqi | NP_038761.1, NM_013733.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1S4Z | NMR | - | C | 210-238 | [»] | |
SMRi | Q9QWF0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 205142, 163 interactors |
ComplexPortali | CPX-570, Chromatin assembly factor 1 complex |
ELMi | Q9QWF0 |
IntActi | Q9QWF0, 17 interactors |
STRINGi | 10090.ENSMUSP00000002914 |
PTM databases
iPTMneti | Q9QWF0 |
PhosphoSitePlusi | Q9QWF0 |
Proteomic databases
EPDi | Q9QWF0 |
jPOSTi | Q9QWF0 |
MaxQBi | Q9QWF0 |
PaxDbi | Q9QWF0 |
PeptideAtlasi | Q9QWF0 |
PRIDEi | Q9QWF0 |
Protocols and materials databases
Antibodypediai | 4198, 218 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000002914; ENSMUSP00000002914; ENSMUSG00000002835 |
GeneIDi | 27221 |
KEGGi | mmu:27221 |
UCSCi | uc008dar.1, mouse |
Organism-specific databases
CTDi | 10036 |
MGIi | MGI:1351331, Chaf1a |
Phylogenomic databases
eggNOGi | KOG4364, Eukaryota |
GeneTreei | ENSGT00440000034888 |
HOGENOMi | CLU_014846_0_0_1 |
InParanoidi | Q9QWF0 |
OMAi | CRKPRKT |
OrthoDBi | 1362011at2759 |
PhylomeDBi | Q9QWF0 |
TreeFami | TF350377 |
Miscellaneous databases
BioGRID-ORCSi | 27221, 7 hits in 19 CRISPR screens |
ChiTaRSi | Chaf1a, mouse |
EvolutionaryTracei | Q9QWF0 |
PROi | PR:Q9QWF0 |
RNActi | Q9QWF0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000002835, Expressed in primary oocyte and 82 other tissues |
ExpressionAtlasi | Q9QWF0, baseline and differential |
Family and domain databases
IDEALi | IID50294 |
InterProi | View protein in InterPro IPR029105, CAF1-p150_C2 IPR029091, CAF1_p150_N IPR022043, CAF1A |
Pfami | View protein in Pfam PF15539, CAF1-p150_C2, 1 hit PF15557, CAF1-p150_N, 1 hit PF12253, CAF1A, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAF1A_MOUSE | |
Accessioni | Q9QWF0Primary (citable) accession number: Q9QWF0 Secondary accession number(s): Q3TU22, Q544M2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families